GREMLIN Database
ROCA2 - 1-pyrroline-5-carboxylate dehydrogenase 2
UniProt: P94391 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 515 (462)
Sequences: 36068 (21791)
Seq/√Len: 1013.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
330_E428_S5.2811.00
330_E426_K3.2931.00
268_E278_Q3.2801.00
302_E339_R3.1901.00
407_D410_A2.8891.00
333_Y426_K2.8831.00
297_E329_H2.8001.00
389_T399_K2.7731.00
59_D62_E2.7431.00
83_A250_K2.7221.00
365_Q369_D2.6861.00
53_V66_R2.6601.00
337_L424_F2.6031.00
109_R142_E2.5661.00
306_Q343_I2.5201.00
377_I411_R2.5011.00
375_I398_I2.4561.00
376_E380_Q2.4221.00
216_K219_E2.4131.00
297_E331_K2.4031.00
83_A252_S2.3941.00
111_R114_E2.3721.00
42_N84_K2.2921.00
383_R405_D2.2141.00
71_S74_H2.1981.00
450_T456_I2.1851.00
362_V371_I2.1771.00
39_L70_A2.1581.00
104_A221_L2.1451.00
53_V63_V2.1431.00
372_M390_G2.0541.00
430_F455_H2.0521.00
372_M376_E2.0441.00
377_I381_E2.0421.00
112_K135_A2.0411.00
222_E228_K2.0271.00
170_T509_K2.0111.00
341_I345_E1.9551.00
100_V226_L1.9421.00
209_A364_D1.9381.00
107_K223_E1.9331.00
243_D247_D1.9161.00
327_V433_A1.8921.00
403_F412_L1.8881.00
293_V308_I1.8781.00
372_M398_I1.8741.00
430_F458_R1.8671.00
458_R462_E1.8621.00
441_E489_M1.8621.00
247_D269_R1.8551.00
298_D455_H1.8221.00
335_Q339_R1.8171.00
169_Y506_M1.8151.00
114_E216_K1.8121.00
113_H131_D1.8111.00
40_V222_E1.8081.00
107_K224_S1.8011.00
169_Y503_A1.7991.00
385_V404_A1.7951.00
464_H488_K1.7811.00
327_V436_V1.7781.00
329_H427_V1.7651.00
379_K384_L1.7651.00
80_Q250_K1.7411.00
107_K220_V1.7301.00
46_V77_Q1.7291.00
76_E244_Y1.7241.00
344_T402_I1.7171.00
265_R268_E1.7011.00
408_P436_V1.7001.00
264_T489_M1.6801.00
107_K110_R1.6761.00
308_I336_V1.6681.00
111_R223_E1.6601.00
86_F90_R1.6551.00
411_R415_E1.6471.00
326_A422_V1.6291.00
413_M425_C1.6281.00
218_V233_F1.6241.00
76_E80_Q1.6171.00
341_I385_V1.6161.00
384_L403_F1.6091.00
201_T230_V1.6031.00
301_I339_R1.5921.00
118_L216_K1.5841.00
432_E435_E1.5821.00
102_F142_E1.5761.00
106_A142_E1.5651.00
338_E342_E1.5621.00
313_F344_T1.5601.00
334_D426_K1.5601.00
333_Y337_L1.5591.00
237_S240_E1.5561.00
141_M193_V1.5531.00
431_D462_E1.5361.00
106_A110_R1.5321.00
297_E455_H1.5251.00
76_E248_H1.5231.00
383_R404_A1.5031.00
428_S432_E1.4781.00
373_S411_R1.4731.00
446_G465_V1.4681.00
93_S96_E1.4601.00
109_R138_I1.4491.00
102_F149_I1.4281.00
365_Q396_Y1.4231.00
63_V66_R1.4171.00
155_K499_P1.4101.00
305_A339_R1.4081.00
376_E379_K1.4061.00
339_R342_E1.4061.00
120_V130_A1.4011.00
327_V427_V1.3981.00
96_E99_A1.3981.00
307_S449_I1.3911.00
219_E223_E1.3911.00
308_I326_A1.3871.00
113_H117_A1.3851.00
147_Q150_E1.3831.00
335_Q338_E1.3821.00
62_E394_K1.3781.00
374_Y417_I1.3761.00
44_E228_K1.3691.00
296_D455_H1.3671.00
103_R106_A1.3651.00
296_D427_V1.3651.00
177_V202_V1.3601.00
141_M189_A1.3581.00
328_V336_V1.3581.00
105_A142_E1.3531.00
60_K394_K1.3491.00
74_H77_Q1.3491.00
333_Y404_A1.3491.00
343_I346_S1.3381.00
86_F201_T1.3371.00
381_E411_R1.3271.00
117_A121_K1.3181.00
91_Y462_E1.3141.00
218_V231_V1.3101.00
117_A353_D1.3081.00
51_K66_R1.3041.00
79_I248_H1.2931.00
56_N59_D1.2881.00
267_F492_T1.2881.00
111_R216_K1.2861.00
429_D432_E1.2831.00
434_L459_A1.2781.00
168_V461_Q1.2751.00
368_Y398_I1.2741.00
371_I419_G1.2711.00
342_E345_E1.2711.00
432_E436_V1.2701.00
221_L231_V1.2661.00
345_E386_S1.2651.00
115_F216_K1.2631.00
348_V356_D1.2591.00
253_L284_I1.2461.00
378_G403_F1.2421.00
427_V436_V1.2381.00
369_D373_S1.2291.00
188_M204_L1.2281.00
40_V45_R1.2261.00
296_D459_A1.2241.00
92_T96_E1.2211.00
55_I63_V1.2041.00
105_A109_R1.2031.00
347_K359_M1.2001.00
127_W131_D1.1971.00
208_S237_S1.1971.00
368_Y372_M1.1961.00
368_Y390_G1.1951.00
334_D338_E1.1891.00
337_L341_I1.1871.00
329_H332_V1.1841.00
414_Q439_N1.1811.00
104_A220_V1.1771.00
155_K500_D1.1711.00
329_H429_D1.1601.00
166_Q457_E1.1581.00
192_T231_V1.1571.00
75_A241_V1.1551.00
104_A226_L1.1491.00
104_A141_M1.1481.00
79_I250_K1.1471.00
169_Y508_A1.1421.00
500_D503_A1.1411.00
350_A357_V1.1401.00
365_Q368_Y1.1381.00
340_V422_V1.1381.00
351_K354_S1.1341.00
243_D269_R1.1311.00
336_V340_V1.1311.00
294_V433_A1.1301.00
446_G468_L1.1291.00
103_R107_K1.1241.00
381_E410_A1.1231.00
408_P439_N1.1231.00
208_S239_A1.1221.00
111_R220_V1.1181.00
120_V352_P1.1181.00
342_E346_S1.1181.00
414_Q441_E1.1131.00
176_V245_L1.1111.00
69_K237_S1.1111.00
56_N351_K1.1111.00
177_V255_T1.1081.00
409_K414_Q1.1051.00
348_V358_Y1.1031.00
80_Q84_K1.1021.00
456_I470_F1.1021.00
243_D265_R1.1011.00
117_A127_W1.1001.00
262_V265_R1.0991.00
294_V459_A1.0971.00
499_P503_A1.0951.00
460_K511_I1.0921.00
332_V335_Q1.0891.00
244_Y248_H1.0881.00
337_L385_V1.0851.00
371_I421_V1.0781.00
58_A352_P1.0771.00
92_T100_V1.0761.00
290_K324_S1.0631.00
110_R223_E1.0631.00
115_F220_V1.0621.00
434_L462_E1.0561.00
379_K403_F1.0461.00
65_G212_V1.0431.00
183_F319_K1.0421.00
500_D508_A1.0421.00
341_I386_S1.0401.00
431_D458_R1.0401.00
373_S377_I1.0381.00
391_D399_K1.0381.00
345_E399_K1.0371.00
292_T446_G1.0371.00
430_F459_A1.0361.00
79_I251_T1.0361.00
267_F283_V1.0311.00
293_V323_G1.0271.00
265_R269_R1.0271.00
300_D451_N1.0231.00
215_A233_F1.0221.00
337_L404_A1.0221.00
229_G232_N1.0211.00
387_G401_T1.0201.00
264_T280_L1.0171.00
387_G399_K1.0151.00
85_A201_T1.0151.00
102_F146_R1.0141.00
294_V437_A1.0091.00
87_E435_E1.0061.00
72_Q244_Y1.0051.00
339_R343_I1.0041.00
112_K131_D1.0011.00
52_I69_K1.0011.00
176_V251_T0.9981.00
172_T253_L0.9981.00
60_K396_Y0.9931.00
368_Y396_Y0.9911.00
215_A235_P0.9901.00
87_E91_Y0.9901.00
261_E265_R0.9901.00
192_T202_V0.9891.00
452_N455_H0.9881.00
334_D337_L0.9841.00
137_A189_A0.9831.00
431_D435_E0.9711.00
174_V251_T0.9591.00
87_E464_H0.9561.00
40_V218_V0.9511.00
168_V511_I0.9511.00
42_N81_A0.9501.00
259_S262_V0.9501.00
56_N121_K0.9491.00
252_S464_H0.9451.00
406_L410_A0.9421.00
81_A232_N0.9381.00
44_E77_Q0.9341.00
427_V433_A0.9321.00
374_Y377_I0.9311.00
485_G494_S0.9291.00
267_F271_A0.9271.00
300_D303_L0.9251.00
447_A473_N0.9251.00
388_G401_T0.9221.00
69_K240_E0.9221.00
433_A436_V0.9211.00
305_A340_V0.9211.00
170_T461_Q0.9171.00
494_S507_Q0.9161.00
296_D430_F0.9141.00
377_I380_Q0.9111.00
302_E335_Q0.9101.00
247_D278_Q0.9101.00
111_R219_E0.9091.00
172_T282_R0.9081.00
125_K133_D0.9061.00
148_M197_V0.9051.00
98_A149_I0.9021.00
487_F507_Q0.9001.00
374_Y411_R0.8991.00
311_S323_G0.8991.00
408_P425_C0.8961.00
295_V336_V0.8931.00
448_V459_A0.8891.00
56_N62_E0.8891.00
98_A145_A0.8891.00
312_A324_S0.8881.00
378_G417_I0.8871.00
425_C436_V0.8871.00
90_R464_H0.8861.00
305_A343_I0.8841.00
121_K353_D0.8831.00
108_V137_A0.8821.00
226_L231_V0.8791.00
282_R507_Q0.8791.00
174_V201_T0.8791.00
304_A308_I0.8771.00
333_Y424_F0.8761.00
172_T200_N0.8751.00
427_V432_E0.8741.00
83_A87_E0.8721.00
219_E222_E0.8671.00
254_I266_I0.8671.00
105_A145_A0.8661.00
39_L235_P0.8661.00
305_A336_V0.8621.00
165_N482_H0.8621.00
75_A176_V0.8591.00
72_Q240_E0.8581.00
305_A309_F0.8541.00
50_A71_S0.8541.00
114_E117_A0.8521.00
301_I305_A0.8501.00
85_A230_V0.8471.00
369_D372_M0.8431.00
371_I417_I0.8431.00
435_E439_N0.8431.00
102_F145_A0.8421.00
123_A210_T0.8411.00
175_T253_L0.8391.00
49_E77_Q0.8381.00
94_P98_A0.8371.00
170_T487_F0.8331.00
333_Y405_D0.8301.00
108_V138_I0.8261.00
382_G410_A0.8261.00
309_F343_I0.8261.00
176_V254_I0.8251.00
293_V449_I0.8251.00
204_L214_A0.8221.00
375_I421_V0.8221.00
446_G463_F0.8211.00
296_D450_T0.8211.00
304_A336_V0.8201.00
267_F280_L0.8191.00
303_L307_S0.8181.00
98_A102_F0.8171.00
239_A243_D0.8171.00
172_T507_Q0.8141.00
72_Q76_E0.8131.00
101_L197_V0.8101.00
99_A103_R0.8101.00
254_I270_A0.8101.00
434_L438_N0.8091.00
137_A185_F0.8081.00
291_D324_S0.8071.00
309_F340_V0.8061.00
103_R224_S0.8041.00
209_A366_G0.8021.00
55_I396_Y0.8021.00
48_T74_H0.8001.00
405_D426_K0.7971.00
373_S376_E0.7961.00
39_L68_S0.7961.00
52_I237_S0.7951.00
482_H510_T0.7951.00
107_K111_R0.7941.00
403_F406_L0.7941.00
73_E76_E0.7931.00
49_E71_S0.7901.00
84_K87_E0.7891.00
246_V266_I0.7881.00
91_Y461_Q0.7861.00
267_F270_A0.7851.00
188_M192_T0.7851.00
371_I398_I0.7811.00
453_R456_I0.7781.00
349_T391_D0.7781.00
292_T437_A0.7731.00
263_G287_M0.7721.00
264_T268_E0.7681.00
58_A397_F0.7661.00
296_D329_H0.7631.00
48_T51_K0.7621.00
457_E461_Q0.7591.00
256_F262_V0.7571.00
120_V127_W0.7551.00
42_N228_K0.7521.00
89_W230_V0.7511.00
77_Q81_A0.7511.00
177_V191_T0.7491.00
347_K358_Y0.7481.00
123_A363_I0.7471.00
189_A217_F0.7471.00
116_S131_D0.7461.00
429_D435_E0.7441.00
45_R222_E0.7441.00
73_E77_Q0.7431.00
263_G285_A0.7411.00
116_S127_W0.7391.00
375_I403_F0.7391.00
200_N506_M0.7391.00
116_S120_V0.7391.00
115_F185_F0.7361.00
194_A501_Y0.7351.00
340_V402_I0.7351.00
81_A229_G0.7341.00
246_V269_R0.7341.00
82_A232_N0.7321.00
49_E73_E0.7311.00
128_N358_Y0.7301.00
102_F106_A0.7291.00
368_Y391_D0.7281.00
374_Y415_E0.7261.00
127_W353_D0.7251.00
338_E341_I0.7211.00
198_T506_M0.7201.00
46_V74_H0.7201.00
379_K389_T0.7201.00
119_L122_E0.7201.00
87_E90_R0.7181.00
465_V468_L0.7181.00
95_E149_I0.7161.00
176_V242_G0.7141.00
329_H430_F0.7131.00
125_K129_E0.7121.00
341_I344_T0.7121.00
70_A74_H0.7111.00
203_V232_N0.7101.00
75_A79_I0.7101.00
365_Q390_G0.7091.00
165_N510_T0.7091.00
206_P235_P0.7081.00
351_K357_V0.7071.00
236_G241_V0.7071.00
386_S402_I0.7071.00
236_G242_G0.7051.00
159_S165_N0.7041.00
77_Q80_Q0.7031.00
201_T232_N0.7021.00
188_M214_A0.7001.00
185_F213_I0.6941.00
328_V332_V0.6941.00
195_P202_V0.6941.00
84_K88_E0.6941.00
131_D135_A0.6921.00
191_T495_K0.6911.00
270_A283_V0.6901.00
48_T68_S0.6881.00
88_E92_T0.6881.00
430_F434_L0.6881.00
75_A244_Y0.6881.00
302_E343_I0.6871.00
49_E74_H0.6871.00
264_T278_Q0.6851.00
56_N353_D0.6851.00
64_V121_K0.6821.00
226_L230_V0.6801.00
246_V254_I0.6801.00
301_I332_V0.6791.00
76_E250_K0.6781.00
70_A234_V0.6781.00
239_A265_R0.6771.00
327_V408_P0.6761.00
215_A219_E0.6761.00
344_T347_K0.6751.00
60_K365_Q0.6741.00
354_S357_V0.6731.00
460_K470_F0.6711.00
118_L212_V0.6711.00
55_I60_K0.6711.00
406_L413_M0.6681.00
349_T359_M0.6681.00
70_A78_A0.6671.00
408_P413_M0.6661.00
298_D450_T0.6661.00
344_T386_S0.6651.00
260_R489_M0.6641.00
109_R135_A0.6631.00
89_W100_V0.6631.00
218_V222_E0.6631.00
204_L231_V0.6621.00
268_E271_A0.6621.00
100_V224_S0.6611.00
340_V424_F0.6591.00
282_R504_L0.6581.00
388_G399_K0.6551.00
326_A336_V0.6551.00
372_M375_I0.6551.00
208_S370_K0.6531.00
366_G369_D0.6501.00
240_E243_D0.6501.00
398_I401_T0.6501.00
209_A363_I0.6491.00
341_I402_I0.6461.00
327_V413_M0.6461.00
308_I340_V0.6451.00
94_P148_M0.6451.00
109_R112_K0.6421.00
436_V439_N0.6421.00
42_N232_N0.6421.00
67_V211_P0.6421.00
221_L226_L0.6401.00
381_E409_K0.6401.00
309_F347_K0.6401.00
90_R170_T0.6391.00
451_N454_K0.6381.00
364_D367_S0.6371.00
83_A86_F0.6371.00
90_R462_E0.6371.00
367_S370_K0.6331.00
125_K183_F0.6331.00
450_T455_H0.6331.00
121_K212_V0.6321.00
100_V225_G0.6321.00
316_A359_M0.6311.00
172_T252_S0.6261.00
88_E91_Y0.6251.00
284_I504_L0.6221.00
106_A109_R0.6201.00
68_S235_P0.6201.00
164_K457_E0.6191.00
42_N77_Q0.6191.00
216_K220_V0.6181.00
366_G370_K0.6181.00
107_K225_G0.6171.00
372_M379_K0.6161.00
417_I421_V0.6161.00
384_L401_T0.6151.00
80_Q83_A0.6151.00
453_R457_E0.6141.00
242_G246_V0.6101.00
295_V304_A0.6091.00
246_V279_H0.6091.00
166_Q511_I0.6081.00
188_M217_F0.6071.00
108_V217_F0.6061.00
456_I460_K0.6061.00
113_H127_W0.6061.00
267_F285_A0.6041.00
94_P197_V0.6041.00
362_V367_S0.6041.00
131_D355_A0.6031.00
134_T138_I0.6031.00
120_V353_D0.6021.00
503_A506_M0.6011.00
192_T204_L0.6011.00
293_V326_A0.6001.00
464_H487_F0.5991.00
406_L412_L0.5981.00
175_T200_N0.5971.00
448_V456_I0.5971.00
390_G399_K0.5961.00
185_F217_F0.5951.00
208_S265_R0.5911.00
100_V104_A0.5911.00
119_L133_D0.5911.00
82_A201_T0.5901.00
105_A138_I0.5901.00
271_A278_Q0.5901.00
165_N512_S0.5891.00
87_E462_E0.5891.00
90_R171_P0.5891.00
179_P188_M0.5881.00
108_V115_F0.5871.00
151_L506_M0.5871.00
503_A508_A0.5861.00
134_T185_F0.5861.00
89_W196_I0.5861.00
330_E333_Y0.5861.00
349_T358_Y0.5851.00
108_V185_F0.5851.00
114_E219_E0.5841.00
292_T465_V0.5841.00
104_A107_K0.5831.00
434_L458_R0.5831.00
41_I78_A0.5811.00
176_V203_V0.5791.00
286_E444_L0.5781.00
434_L463_F0.5781.00
191_T255_T0.5761.00
328_V424_F0.5761.00
133_D183_F0.5761.00
119_L213_I0.5761.00
299_A304_A0.5751.00
301_I335_Q0.5731.00
468_L509_K0.5721.00
253_L504_L0.5711.00
54_S65_G0.5711.00
117_A120_V0.5701.00
54_S211_P0.5701.00
362_V398_I0.5681.00
297_E427_V0.5681.00
54_S122_E0.5671.00
370_K373_S0.5661.00
43_G222_E0.5641.00
105_A141_M0.5641.00
115_F134_T0.5641.00
151_L502_L0.5641.00
375_I401_T0.5641.00
146_R149_I0.5631.00
119_L130_A0.5611.00
85_A88_E0.5611.00
168_V509_K0.5591.00
332_V427_V0.5591.00
413_M436_V0.5591.00
298_D451_N0.5571.00
381_E407_D0.5571.00
108_V141_M0.5561.00
319_K322_A0.5541.00
404_A426_K0.5531.00
177_V192_T0.5531.00
78_A234_V0.5531.00
45_R219_E0.5531.00
298_D452_N0.5521.00
72_Q247_D0.5511.00
454_K458_R0.5491.00
303_L451_N0.5491.00
384_L387_G0.5481.00
96_E100_V0.5481.00
293_V447_A0.5451.00
198_T502_L0.5411.00
463_F468_L0.5411.00
303_L306_Q0.5391.00
244_Y247_D0.5381.00
237_S241_V0.5371.00
40_V43_G0.5351.00
39_L48_T0.5341.00
125_K130_A0.5341.00
261_E366_G0.5331.00
64_V212_V0.5331.00
136_E186_A0.5321.00
112_K116_S0.5311.00
326_A424_F0.5311.00
186_A190_G0.5311.00
51_K68_S0.5301.00
81_A85_A0.5291.00
464_H507_Q0.5291.00
323_G447_A0.5281.00
144_Y194_A0.5281.00
208_S261_E0.5281.00
175_T202_V0.5271.00
313_F347_K0.5271.00
217_F221_L0.5271.00
321_S477_A0.5261.00
83_A174_V0.5251.00
454_K457_E0.5251.00
291_D445_T0.5241.00
311_S473_N0.5231.00
103_R225_G0.5231.00
337_L402_I0.5221.00
154_G168_V0.5221.00
174_V252_S0.5221.00
305_A332_V0.5221.00
67_V212_V0.5221.00
143_Y147_Q0.5221.00
82_A203_V0.5211.00
445_T469_Y0.5201.00
222_E225_G0.5201.00
175_T255_T0.5191.00
384_L388_G0.5171.00
312_A422_V0.5171.00
203_V234_V0.5131.00
412_L417_I0.5131.00
350_A354_S0.5121.00
135_A138_I0.5111.00
469_Y472_R0.5091.00
379_K382_G0.5081.00
123_A315_F0.5081.00
79_I244_Y0.5081.00
116_S130_A0.5061.00
112_K138_I0.5061.00
41_I46_V0.5051.00
260_R264_T0.5051.00
487_F494_S0.5041.00
86_F230_V0.5041.00
448_V470_F0.5041.00
374_Y412_L0.5031.00
430_F462_E0.5031.00
81_A228_K0.5011.00
338_E345_E0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4nm9A 2 0.9981 100 0.067 Contact Map
1uzbA 5 0.9981 100 0.073 Contact Map
3qanA 2 0.9845 100 0.073 Contact Map
4jdcA 5 0.9786 100 0.093 Contact Map
4pz2A 4 0.9165 100 0.093 Contact Map
4oe6A 5 0.9029 100 0.096 Contact Map
4o5hA 4 0.967 100 0.097 Contact Map
4yweA 5 0.9068 100 0.097 Contact Map
4pxlA 4 0.9184 100 0.099 Contact Map
4lihA 5 0.9398 100 0.099 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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