SP0M - BACSU - GREMLIN Contacts

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SP0M - Sporulation-control protein spo0M

UniProt:	P71088	Sequences: MSFFKKLAASAGIGAAKVDTILEKDAYFPGEEVQGTVHVKGGKIAQDIRYIDLQLSTRYVIVKDDEEHRKYATIHSFRVTGSFTIQPGEEHQFPFTFTLPLDTPITVGKVEVAVVTDLDIQGGIDKSDHDRIFVEAHPWIENVLEAIENLGFRLNEADCEQAPYFQRRLPFVQEFEFVPTSGYYRQMLDELELIFLLDEDGLEIIFEVDRRARGLRGWLEEMYNDGEQLVRVRFSQSELEDTEELEEVLEEILDQYAE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	258 (245)
Sequences:	644 (439)
Seq/√Len:	28.0

BACSU - SP0M

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
147_I	191_L	5.432	1.00
36_T	92_Q	5.328	1.00
45_A	87_P	4.981	1.00
45_A	86_Q	3.502	1.00
16_A	46_Q	3.444	1.00
63_K	68_H	3.384	1.00
155_N	178_V	3.326	1.00
35_G	95_F	3.265	1.00
23_E	34_Q	3.238	1.00
40_K	90_E	3.224	1.00
28_F	31_E	3.061	1.00
49_R	119_D	2.985	1.00
83_F	91_H	2.617	0.99
106_T	112_V	2.605	0.99
123_G	126_K	2.478	0.99
140_I	195_F	2.437	0.99
246_E	250_E	2.219	0.98
9_A	44_I	2.215	0.98
27_Y	31_E	2.146	0.98
23_E	36_T	2.135	0.98
9_A	46_Q	2.109	0.97
62_V	67_E	2.090	0.97
21_I	38_H	2.088	0.97
9_A	16_A	2.008	0.96
47_D	84_T	1.955	0.96
141_E	145_E	1.913	0.95
3_F	7_L	1.862	0.95
137_H	197_L	1.846	0.94
24_K	31_E	1.809	0.94
39_V	85_I	1.806	0.94
192_E	209_D	1.799	0.93
247_E	250_E	1.799	0.93
162_A	172_V	1.791	0.93
124_I	177_F	1.746	0.92
39_V	93_F	1.685	0.91
103_T	157_A	1.683	0.91
102_D	168_R	1.658	0.90
116_T	130_D	1.623	0.89
10_S	46_Q	1.611	0.89
190_E	209_D	1.605	0.89
207_E	229_L	1.595	0.88
17_K	40_K	1.591	0.88
29_P	135_E	1.571	0.88
189_D	221_E	1.560	0.87
26_A	135_E	1.522	0.86
6_K	93_F	1.505	0.85
103_T	134_V	1.475	0.84
60_V	158_D	1.456	0.83
24_K	27_Y	1.452	0.83
57_T	159_C	1.447	0.83
33_V	187_M	1.443	0.83
234_F	238_E	1.443	0.83
73_T	76_S	1.441	0.83
39_V	118_L	1.439	0.83
20_T	114_V	1.433	0.82
34_Q	96_T	1.429	0.82
4_F	149_N	1.373	0.79
213_R	217_G	1.366	0.79
55_L	134_V	1.365	0.79
114_V	171_F	1.363	0.79
188_L	191_L	1.354	0.78
38_H	90_E	1.330	0.77
240_E	247_E	1.323	0.77
164_Y	172_V	1.317	0.76
55_L	99_L	1.313	0.76
176_E	192_E	1.311	0.76
57_T	112_V	1.311	0.76
172_V	194_I	1.307	0.76
113_A	210_R	1.307	0.76
193_L	230_V	1.300	0.76
60_V	67_E	1.299	0.75
20_T	158_D	1.291	0.75
96_T	106_T	1.281	0.74
147_I	193_L	1.266	0.74
49_R	126_K	1.265	0.74
59_Y	159_C	1.259	0.73
59_Y	171_F	1.256	0.73
51_I	71_Y	1.236	0.72
62_V	65_D	1.235	0.72
42_G	120_I	1.229	0.71
86_Q	89_E	1.226	0.71
82_S	91_H	1.213	0.70
62_V	174_E	1.208	0.70
212_A	221_E	1.208	0.70
119_D	217_G	1.204	0.70
134_V	189_D	1.192	0.69
51_I	82_S	1.185	0.68
85_I	91_H	1.170	0.67
99_L	132_I	1.156	0.66
160_E	177_F	1.155	0.66
63_K	66_E	1.151	0.66
48_I	120_I	1.139	0.65
19_D	89_E	1.124	0.64
202_L	227_E	1.118	0.64
56_S	115_V	1.108	0.63
35_G	94_P	1.101	0.63
16_A	39_V	1.097	0.62
144_L	177_F	1.094	0.62
29_P	138_P	1.084	0.61
144_L	157_A	1.082	0.61
14_G	19_D	1.079	0.61
198_D	251_E	1.069	0.60
221_E	226_G	1.062	0.60
4_F	106_T	1.053	0.59
128_D	174_E	1.048	0.59
227_E	234_F	1.048	0.59
18_V	100_P	1.046	0.59
228_Q	231_R	1.045	0.59
52_D	117_D	1.023	0.57
113_A	129_H	1.018	0.57
212_A	215_L	1.017	0.56
61_I	70_K	1.006	0.56
27_Y	99_L	0.991	0.54
188_L	212_A	0.985	0.54
174_E	209_D	0.979	0.54
193_L	206_F	0.978	0.53
24_K	33_V	0.974	0.53
26_A	133_F	0.971	0.53
159_C	170_P	0.964	0.52
127_S	163_P	0.950	0.51
154_L	157_A	0.948	0.51
77_F	105_I	0.948	0.51
139_W	202_L	0.938	0.50
206_F	232_V	0.936	0.50
16_A	42_G	0.933	0.50
231_R	234_F	0.924	0.49
31_E	89_E	0.922	0.49
159_C	171_F	0.921	0.49
29_P	140_I	0.921	0.49
146_A	176_E	0.911	0.48
40_K	47_D	0.909	0.48
47_D	82_S	0.908	0.48
79_V	144_L	0.907	0.48
48_I	145_E	0.906	0.48
156_E	207_E	0.905	0.48
54_Q	73_T	0.903	0.48
115_V	127_S	0.902	0.48
153_R	178_V	0.898	0.47
100_P	171_F	0.897	0.47
16_A	44_I	0.893	0.47
37_V	43_K	0.893	0.47
62_V	66_E	0.887	0.46
46_Q	120_I	0.886	0.46
223_Y	226_G	0.884	0.46
174_E	207_E	0.883	0.46
166_Q	203_E	0.880	0.46
40_K	201_G	0.877	0.46
55_L	169_L	0.872	0.45
222_M	231_R	0.870	0.45
33_V	138_P	0.858	0.44
59_Y	170_P	0.858	0.44
140_I	197_L	0.858	0.44
180_T	193_L	0.855	0.44
28_F	141_E	0.853	0.44
134_V	206_F	0.852	0.44
38_H	92_Q	0.851	0.44
160_E	207_E	0.850	0.44
103_T	114_V	0.850	0.44
28_F	134_V	0.849	0.44
175_F	217_G	0.849	0.44
34_Q	99_L	0.848	0.44
209_D	214_G	0.846	0.43
144_L	196_L	0.840	0.43
122_G	148_E	0.838	0.43
17_K	180_T	0.836	0.43
110_V	159_C	0.836	0.43
51_I	115_V	0.833	0.42
32_E	96_T	0.828	0.42
45_A	92_Q	0.825	0.42
49_R	123_G	0.822	0.42
214_G	217_G	0.817	0.41
177_F	186_Q	0.817	0.41
52_D	78_R	0.813	0.41
110_V	154_L	0.812	0.41
195_F	204_I	0.811	0.41
45_A	78_R	0.804	0.40
204_I	232_V	0.803	0.40
179_P	212_A	0.803	0.40
7_L	206_F	0.801	0.40
143_V	202_L	0.798	0.40
25_D	168_R	0.797	0.40
164_Y	167_R	0.795	0.40
236_Q	239_L	0.791	0.39
22_L	39_V	0.785	0.39
129_H	183_Y	0.783	0.39
19_D	190_E	0.779	0.38
5_K	207_E	0.775	0.38
189_D	226_G	0.772	0.38
45_A	84_T	0.769	0.38
120_I	152_F	0.769	0.38
19_D	130_D	0.764	0.37
5_K	25_D	0.763	0.37
118_L	186_Q	0.762	0.37
103_T	112_V	0.762	0.37
4_F	93_F	0.757	0.37
16_A	53_L	0.757	0.37
198_D	201_G	0.751	0.36
44_I	75_H	0.749	0.36
189_D	211_R	0.746	0.36
77_F	97_F	0.744	0.36
25_D	81_G	0.741	0.36
57_T	177_F	0.739	0.36
21_I	36_T	0.737	0.35
175_F	193_L	0.736	0.35
43_K	87_P	0.735	0.35
64_D	93_F	0.728	0.35
181_S	206_F	0.726	0.35
107_V	147_I	0.722	0.34
167_R	248_V	0.718	0.34
212_A	217_G	0.717	0.34
29_P	137_H	0.714	0.34
78_R	111_E	0.713	0.34
204_I	225_D	0.708	0.33
70_K	191_L	0.708	0.33
81_G	123_G	0.706	0.33
155_N	203_E	0.702	0.33
44_I	120_I	0.702	0.33
225_D	233_R	0.697	0.33
145_E	149_N	0.697	0.33
46_Q	85_I	0.695	0.33
43_K	231_R	0.692	0.32
27_Y	33_V	0.692	0.32
229_L	232_V	0.689	0.32
164_Y	194_I	0.687	0.32
5_K	13_I	0.685	0.32
79_V	162_A	0.685	0.32
39_V	181_S	0.684	0.32
245_L	249_L	0.684	0.32
158_D	172_V	0.681	0.32
79_V	149_N	0.680	0.32
30_G	173_Q	0.680	0.32
176_E	209_D	0.678	0.31
42_G	118_L	0.677	0.31
113_A	136_A	0.675	0.31
98_T	201_G	0.673	0.31
152_F	188_L	0.673	0.31
189_D	210_R	0.673	0.31
213_R	220_E	0.664	0.30
64_D	67_E	0.664	0.30
113_A	197_L	0.663	0.30
48_I	88_G	0.662	0.30
43_K	88_G	0.660	0.30
51_I	79_V	0.659	0.30
111_E	145_E	0.659	0.30
57_T	106_T	0.659	0.30
74_I	152_F	0.658	0.30
120_I	222_M	0.658	0.30
3_F	112_V	0.657	0.30
154_L	175_F	0.656	0.30
112_V	155_N	0.655	0.30
58_R	129_H	0.654	0.30
59_Y	164_Y	0.650	0.30
4_F	22_L	0.649	0.29
157_A	204_I	0.648	0.29
33_V	155_N	0.648	0.29
99_L	106_T	0.645	0.29
213_R	216_R	0.642	0.29
20_T	53_L	0.641	0.29
163_P	167_R	0.639	0.29
95_F	204_I	0.635	0.29
191_L	196_L	0.635	0.29
32_E	132_I	0.630	0.28
245_L	248_V	0.629	0.28
48_I	200_D	0.628	0.28
83_F	89_E	0.626	0.28
43_K	133_F	0.623	0.28
220_E	224_N	0.623	0.28
112_V	177_F	0.622	0.28
191_L	249_L	0.621	0.28
240_E	252_I	0.620	0.28
214_G	218_W	0.620	0.28
171_F	203_E	0.619	0.28
59_Y	72_A	0.619	0.28
80_T	85_I	0.619	0.28
144_L	183_Y	0.618	0.28
164_Y	205_I	0.617	0.27
93_F	97_F	0.616	0.27
155_N	190_E	0.616	0.27
65_D	96_T	0.613	0.27
85_I	111_E	0.610	0.27
208_V	211_R	0.610	0.27
222_M	226_G	0.609	0.27
239_L	251_E	0.607	0.27
5_K	43_K	0.606	0.27
229_L	234_F	0.605	0.27
16_A	112_V	0.605	0.27
53_L	114_V	0.602	0.27
76_S	150_L	0.600	0.26
46_Q	112_V	0.598	0.26
207_E	220_E	0.597	0.26
181_S	240_E	0.597	0.26
179_P	188_L	0.596	0.26
19_D	37_V	0.595	0.26
3_F	162_A	0.593	0.26
93_F	248_V	0.592	0.26
73_T	143_V	0.592	0.26
97_F	136_A	0.585	0.26
105_I	202_L	0.583	0.25
18_V	97_F	0.577	0.25
28_F	249_L	0.577	0.25
138_P	229_L	0.576	0.25
102_D	196_L	0.568	0.25
220_E	223_Y	0.566	0.24
3_F	170_P	0.566	0.24
27_Y	114_V	0.565	0.24
13_I	209_D	0.565	0.24
114_V	136_A	0.565	0.24
17_K	142_N	0.565	0.24
18_V	125_D	0.563	0.24
101_L	199_E	0.562	0.24
115_V	249_L	0.562	0.24
8_A	58_R	0.558	0.24
11_A	101_L	0.555	0.24
106_T	207_E	0.555	0.24
34_Q	120_I	0.553	0.24
137_H	140_I	0.552	0.24
239_L	249_L	0.552	0.24
35_G	195_F	0.552	0.24
25_D	247_E	0.551	0.24
37_V	189_D	0.551	0.24
26_A	141_E	0.550	0.24
35_G	77_F	0.550	0.24
226_G	231_R	0.550	0.24
189_D	230_V	0.550	0.24
57_T	113_A	0.549	0.24
61_I	164_Y	0.548	0.23
11_A	142_N	0.547	0.23
53_L	155_N	0.546	0.23
112_V	159_C	0.545	0.23
39_V	187_M	0.544	0.23
48_I	225_D	0.540	0.23
53_L	199_E	0.540	0.23
6_K	214_G	0.537	0.23
102_D	204_I	0.537	0.23
53_L	95_F	0.532	0.23
29_P	101_L	0.529	0.22
61_I	65_D	0.528	0.22
73_T	87_P	0.528	0.22
158_D	192_E	0.526	0.22
101_L	204_I	0.525	0.22
122_G	186_Q	0.525	0.22
119_D	152_F	0.523	0.22
235_S	240_E	0.522	0.22
9_A	69_R	0.521	0.22
166_Q	179_P	0.521	0.22
248_V	252_I	0.520	0.22
53_L	132_I	0.518	0.22
112_V	141_E	0.517	0.22
124_I	138_P	0.517	0.22
161_Q	200_D	0.516	0.22
13_I	188_L	0.514	0.22
203_E	231_R	0.514	0.22
75_H	217_G	0.512	0.22
34_Q	64_D	0.512	0.22
148_E	251_E	0.511	0.21
51_I	163_P	0.511	0.21
185_R	195_F	0.511	0.21
41_G	136_A	0.510	0.21
22_L	144_L	0.510	0.21
144_L	175_F	0.510	0.21
54_Q	252_I	0.508	0.21
15_A	120_I	0.508	0.21
188_L	226_G	0.508	0.21
63_K	191_L	0.507	0.21
59_Y	172_V	0.506	0.21
45_A	82_S	0.505	0.21
43_K	181_S	0.503	0.21
46_Q	126_K	0.500	0.21

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4r7xA	1	0.5078	99.3	0.836	Contact Map
4ll1A	2	0.8101	99.1	0.848	Contact Map
4geiA	1	0.4767	98.7	0.869	Contact Map
2fauA	1	0.8333	98.5	0.879	Contact Map
4r7vA	1	0.5078	98.2	0.887	Contact Map
1sujA	1	0.8682	96.6	0.916	Contact Map
1g4mA	1	0.8682	96.2	0.92	Contact Map
3uguA	1	0.845	96.1	0.92	Contact Map
2r51A	2	0.5194	95.3	0.925	Contact Map
4zwjA	1	0.5039	91.5	0.935	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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