PLSX - BACSU - GREMLIN Contacts

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PLSX - Phosphate acyltransferase

UniProt:	P71018	Sequences: MRIAVDAMGGDHAPKAVIDGVIKGIEAFDDLHITLVGDKTTIESHLTTTSDRITVLHADEVIEPTDEPVRAVRRKKNSSMVLMAQEVAENRADACISAGNTGALMTAGLFIVGRIKGIDRPALAPTLPTVSGDGFLLLDVGANVDAKPEHLVQYAIMGSVYSQQVRGVTSPRVGLLNVGTEDKKGNELTKQTFQILKETANINFIGNVEARDLLDDVADVVVTDGFTGNVTLKTLEGSALSIFKMMRDVMTSTLTSKLAAAVLKPKLKEMKMKMEYSNYGGASLFGLKAPVIKAHGSSDSNAVFHAIRQAREMVSQNVAALIQEEVKEEKTDE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	333 (324)
Sequences:	2534 (1654)
Seq/√Len:	91.9

ECOLI - PLSX
BACSU - PLSX

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
39_K	43_E	5.198	1.00
317_N	320_A	4.780	1.00
59_D	77_N	3.665	1.00
83_M	95_C	3.106	1.00
182_D	197_K	2.877	1.00
277_S	299_D	2.870	1.00
32_H	54_T	2.794	1.00
27_A	304_F	2.757	1.00
87_V	92_A	2.718	1.00
39_K	57_H	2.718	1.00
38_D	41_T	2.630	1.00
5_V	21_V	2.593	1.00
42_I	55_V	2.529	1.00
38_D	57_H	2.508	1.00
36_V	86_E	2.458	1.00
16_A	19_D	2.394	1.00
85_Q	89_E	2.305	1.00
34_T	92_A	2.293	1.00
25_I	52_R	2.216	1.00
193_F	204_F	2.144	1.00
311_R	315_S	2.090	1.00
63_E	66_D	2.059	1.00
77_N	82_L	2.042	1.00
223_T	227_T	2.041	1.00
204_F	207_N	2.028	1.00
2_R	32_H	1.956	1.00
176_L	231_T	1.914	1.00
186_N	189_T	1.906	1.00
205_I	218_A	1.898	1.00
159_S	173_V	1.857	1.00
253_T	256_S	1.835	1.00
174_G	208_V	1.831	1.00
251_T	257_K	1.815	1.00
146_A	154_Y	1.812	1.00
24_G	307_I	1.810	1.00
162_S	166_R	1.798	1.00
288_K	323_Q	1.793	1.00
119_D	149_E	1.781	1.00
4_A	92_A	1.767	1.00
23_K	304_F	1.738	1.00
24_G	31_L	1.737	1.00
80_M	104_L	1.715	1.00
43_E	55_V	1.710	1.00
163_Q	169_T	1.707	1.00
164_Q	321_L	1.691	1.00
21_V	33_I	1.682	1.00
45_H	48_T	1.672	1.00
159_S	163_Q	1.671	1.00
144_V	178_V	1.657	1.00
208_V	218_A	1.652	1.00
182_D	193_F	1.638	1.00
212_D	217_V	1.636	1.00
56_L	91_R	1.628	1.00
187_E	191_Q	1.626	1.00
255_T	265_P	1.623	1.00
158_G	220_V	1.589	1.00
28_F	308_R	1.587	1.00
36_V	58_A	1.579	1.00
15_K	19_D	1.570	1.00
268_K	271_K	1.561	1.00
123_L	293_K	1.555	1.00
58_A	82_L	1.553	1.00
34_T	56_L	1.552	1.00
54_T	91_R	1.552	1.00
135_F	221_V	1.547	1.00
60_E	77_N	1.530	1.00
2_R	93_D	1.528	1.00
152_V	195_I	1.527	1.00
27_A	308_R	1.524	1.00
28_F	311_R	1.516	1.00
108_G	291_V	1.512	1.00
194_Q	198_E	1.491	1.00
235_L	238_S	1.487	1.00
259_A	262_V	1.486	1.00
7_A	35_L	1.486	1.00
96_I	310_A	1.485	1.00
129_T	135_F	1.481	1.00
170_S	203_N	1.474	1.00
16_A	300_S	1.473	1.00
214_L	235_L	1.454	1.00
227_T	230_V	1.451	1.00
254_L	257_K	1.449	0.99
86_E	91_R	1.424	0.99
82_L	85_Q	1.422	0.99
104_L	291_V	1.421	0.99
278_N	301_N	1.418	0.99
4_A	83_M	1.413	0.99
137_L	140_V	1.400	0.99
213_L	231_T	1.394	0.99
84_A	107_A	1.394	0.99
155_A	196_L	1.385	0.99
15_K	45_H	1.374	0.99
145_D	186_N	1.373	0.99
147_K	150_H	1.370	0.99
127_L	137_L	1.357	0.99
38_D	58_A	1.344	0.99
60_E	75_K	1.341	0.99
172_R	205_I	1.332	0.99
34_T	86_E	1.317	0.99
41_T	44_S	1.315	0.99
248_D	273_K	1.300	0.99
130_V	244_K	1.298	0.99
109_L	114_R	1.286	0.99
281_G	292_I	1.281	0.99
13_A	16_A	1.278	0.99
81_V	111_I	1.261	0.99
30_D	311_R	1.259	0.99
136_L	162_S	1.246	0.98
250_M	260_A	1.245	0.98
168_V	172_R	1.244	0.98
101_T	123_L	1.243	0.98
4_A	87_V	1.235	0.98
294_A	306_A	1.231	0.98
188_L	192_T	1.227	0.98
193_F	207_N	1.216	0.98
25_I	53_I	1.212	0.98
104_L	293_K	1.209	0.98
36_V	82_L	1.206	0.98
176_L	221_V	1.195	0.98
136_L	166_R	1.194	0.98
7_A	42_I	1.190	0.98
191_Q	194_Q	1.187	0.98
250_M	263_L	1.187	0.98
294_A	303_V	1.181	0.98
84_A	112_V	1.180	0.98
125_P	140_V	1.157	0.98
256_S	266_K	1.153	0.97
31_L	311_R	1.149	0.97
173_V	202_I	1.147	0.97
119_D	150_H	1.133	0.97
34_T	54_T	1.127	0.97
159_S	202_I	1.126	0.97
152_V	156_I	1.125	0.97
272_M	278_N	1.122	0.97
254_L	258_L	1.115	0.97
277_S	301_N	1.111	0.97
43_E	50_S	1.105	0.97
2_R	34_T	1.090	0.96
19_D	300_S	1.090	0.96
124_A	136_L	1.089	0.96
25_I	33_I	1.086	0.96
85_Q	111_I	1.084	0.96
17_V	303_V	1.081	0.96
246_M	270_M	1.069	0.96
30_D	315_S	1.065	0.96
211_R	276_Y	1.063	0.96
130_V	241_S	1.059	0.96
212_D	215_D	1.057	0.96
249_V	269_E	1.053	0.96
119_D	153_Q	1.053	0.96
250_M	256_S	1.042	0.95
283_S	313_M	1.035	0.95
294_A	298_S	1.033	0.95
162_S	220_V	1.033	0.95
280_G	294_A	1.031	0.95
98_A	303_V	1.030	0.95
148_P	191_Q	1.030	0.95
148_P	192_T	1.026	0.95
44_S	55_V	1.017	0.95
66_D	75_K	1.017	0.95
320_A	323_Q	1.009	0.95
63_E	75_K	1.008	0.94
129_T	216_D	1.006	0.94
257_K	264_K	0.998	0.94
205_I	217_V	0.996	0.94
298_S	302_A	0.988	0.94
20_G	303_V	0.985	0.94
38_D	59_D	0.978	0.94
292_I	310_A	0.974	0.93
3_I	6_D	0.970	0.93
2_R	92_A	0.970	0.93
23_K	300_S	0.965	0.93
112_V	289_A	0.965	0.93
247_R	251_T	0.965	0.93
162_S	168_V	0.964	0.93
175_L	207_N	0.964	0.93
5_V	33_I	0.964	0.93
13_A	19_D	0.959	0.93
255_T	266_K	0.958	0.93
160_V	164_Q	0.956	0.93
218_A	221_V	0.956	0.93
196_L	202_I	0.954	0.93
84_A	95_C	0.952	0.93
214_L	234_T	0.952	0.93
70_R	74_R	0.950	0.93
82_L	86_E	0.948	0.92
152_V	155_A	0.947	0.92
264_K	268_K	0.943	0.92
208_V	221_V	0.943	0.92
151_L	192_T	0.941	0.92
20_G	300_S	0.941	0.92
249_V	252_S	0.940	0.92
137_L	231_T	0.938	0.92
152_V	196_L	0.931	0.92
86_E	92_A	0.931	0.92
174_G	205_I	0.928	0.92
13_A	17_V	0.927	0.92
138_L	158_G	0.926	0.92
62_I	71_A	0.922	0.91
240_L	276_Y	0.921	0.91
261_A	264_K	0.921	0.91
183_K	190_K	0.920	0.91
18_I	22_I	0.920	0.91
88_A	113_G	0.919	0.91
304_F	308_R	0.917	0.91
21_V	35_L	0.913	0.91
322_I	326_V	0.908	0.91
163_Q	325_E	0.906	0.91
115_I	319_A	0.904	0.91
305_H	308_R	0.897	0.90
62_I	78_S	0.894	0.90
80_M	100_N	0.893	0.90
122_A	138_L	0.893	0.90
258_L	262_V	0.892	0.90
41_T	45_H	0.891	0.90
90_N	289_A	0.891	0.90
7_A	18_I	0.889	0.90
245_M	249_V	0.888	0.90
126_T	166_R	0.888	0.90
19_D	23_K	0.887	0.90
152_V	192_T	0.884	0.90
234_T	238_S	0.881	0.89
193_F	197_K	0.880	0.89
174_G	218_A	0.878	0.89
276_Y	297_S	0.877	0.89
56_L	86_E	0.876	0.89
106_T	110_F	0.875	0.89
112_V	291_V	0.875	0.89
317_N	321_L	0.875	0.89
78_S	81_V	0.873	0.89
148_P	195_I	0.868	0.89
107_A	111_I	0.865	0.89
253_T	257_K	0.864	0.88
259_A	266_K	0.863	0.88
321_L	324_E	0.856	0.88
81_V	85_Q	0.856	0.88
80_M	293_K	0.854	0.88
49_T	52_R	0.852	0.88
263_L	266_K	0.851	0.88
46_L	55_V	0.848	0.88
144_V	227_T	0.848	0.88
324_E	327_K	0.847	0.87
152_V	200_A	0.846	0.87
81_V	107_A	0.845	0.87
95_C	104_L	0.845	0.87
245_M	273_K	0.843	0.87
18_I	46_L	0.838	0.87
42_I	46_L	0.836	0.87
260_A	264_K	0.835	0.87
96_I	292_I	0.834	0.87
214_L	238_S	0.834	0.87
256_S	262_V	0.833	0.87
75_K	78_S	0.833	0.87
87_V	289_A	0.832	0.87
36_V	56_L	0.832	0.87
249_V	273_K	0.830	0.86
129_T	133_D	0.826	0.86
16_A	299_D	0.818	0.86
250_M	267_L	0.818	0.86
180_T	293_K	0.817	0.86
23_K	26_E	0.816	0.85
135_F	213_L	0.815	0.85
96_I	306_A	0.810	0.85
87_V	90_N	0.807	0.85
231_T	234_T	0.807	0.85
10_G	13_A	0.801	0.84
62_I	81_V	0.800	0.84
40_T	44_S	0.798	0.84
43_E	47_T	0.798	0.84
97_S	104_L	0.794	0.84
251_T	260_A	0.794	0.84
192_T	196_L	0.794	0.84
131_S	172_R	0.792	0.84
22_I	53_I	0.786	0.83
280_G	298_S	0.786	0.83
66_D	71_A	0.779	0.83
249_V	266_K	0.775	0.82
22_I	46_L	0.772	0.82
113_G	288_K	0.768	0.82
210_A	234_T	0.762	0.81
126_T	136_L	0.762	0.81
34_T	91_R	0.757	0.81
160_V	325_E	0.756	0.81
33_I	53_I	0.752	0.80
57_H	82_L	0.748	0.80
227_T	231_T	0.747	0.80
177_N	293_K	0.743	0.80
59_D	82_L	0.740	0.79
159_S	203_N	0.739	0.79
126_T	162_S	0.739	0.79
28_F	31_L	0.738	0.79
18_I	45_H	0.738	0.79
5_V	96_I	0.737	0.79
211_R	297_S	0.733	0.79
149_E	191_Q	0.732	0.79
172_R	220_V	0.731	0.79
10_G	17_V	0.728	0.78
94_A	310_A	0.727	0.78
145_D	189_T	0.724	0.78
18_I	41_T	0.724	0.78
17_V	21_V	0.724	0.78
41_T	57_H	0.720	0.78
124_A	285_F	0.720	0.78
130_V	215_D	0.719	0.77
109_L	113_G	0.718	0.77
275_E	301_N	0.716	0.77
167_G	312_E	0.714	0.77
146_A	224_D	0.714	0.77
159_S	220_V	0.713	0.77
178_V	230_V	0.713	0.77
290_P	313_M	0.712	0.77
23_K	27_A	0.712	0.77
180_T	210_A	0.705	0.76
242_I	246_M	0.703	0.76
127_L	135_F	0.701	0.76
172_R	217_V	0.701	0.76
157_M	322_I	0.700	0.76
68_P	71_A	0.696	0.75
80_M	95_C	0.695	0.75
145_D	187_E	0.694	0.75
72_V	81_V	0.693	0.75
84_A	108_G	0.691	0.75
60_E	76_K	0.684	0.74
302_A	305_H	0.682	0.74
25_I	51_D	0.680	0.73
260_A	267_L	0.676	0.73
105_M	123_L	0.676	0.73
66_D	100_N	0.674	0.73
7_A	10_G	0.673	0.73
259_A	264_K	0.672	0.73
150_H	154_Y	0.670	0.72
7_A	33_I	0.670	0.72
149_E	153_Q	0.669	0.72
68_P	102_G	0.667	0.72
255_T	262_V	0.666	0.72
154_Y	178_V	0.660	0.71
250_M	266_K	0.658	0.71
12_H	17_V	0.657	0.71
18_I	21_V	0.656	0.71
72_V	107_A	0.655	0.71
42_I	45_H	0.655	0.71
250_M	259_A	0.654	0.71
105_M	146_A	0.654	0.71
287_L	293_K	0.654	0.71
252_S	256_S	0.650	0.70
172_R	212_D	0.650	0.70
105_M	143_N	0.648	0.70
208_V	217_V	0.646	0.70
177_N	189_T	0.646	0.70
105_M	121_P	0.638	0.69
131_S	216_D	0.638	0.69
172_R	218_A	0.637	0.69
255_T	259_A	0.631	0.68
160_V	326_V	0.627	0.67
172_R	219_D	0.625	0.67
258_L	261_A	0.622	0.67
174_G	212_D	0.617	0.66
320_A	324_E	0.616	0.66
5_V	17_V	0.616	0.66
112_V	287_L	0.616	0.66
313_M	318_V	0.616	0.66
18_I	53_I	0.616	0.66
122_A	154_Y	0.615	0.66
2_R	91_R	0.614	0.66
310_A	314_V	0.613	0.66
250_M	253_T	0.612	0.65
251_T	255_T	0.611	0.65
186_N	227_T	0.610	0.65
308_R	312_E	0.610	0.65
110_F	113_G	0.609	0.65
35_L	53_I	0.609	0.65
165_V	318_V	0.606	0.65
300_S	304_F	0.604	0.64
140_V	276_Y	0.604	0.64
298_S	303_V	0.603	0.64
58_A	61_V	0.603	0.64
256_S	259_A	0.598	0.64
13_A	98_A	0.598	0.64
245_M	270_M	0.598	0.64
24_G	28_F	0.596	0.63
143_N	154_Y	0.596	0.63
299_D	302_A	0.595	0.63
69_V	73_R	0.595	0.63
118_I	153_Q	0.594	0.63
230_V	234_T	0.592	0.63
104_L	177_N	0.591	0.63
276_Y	302_A	0.591	0.63
84_A	111_I	0.590	0.63
10_G	14_P	0.590	0.63
251_T	264_K	0.587	0.62
25_I	31_L	0.586	0.62
67_E	70_R	0.586	0.62
22_I	49_T	0.585	0.62
249_V	267_L	0.585	0.62
3_I	94_A	0.583	0.62
240_L	247_R	0.582	0.62
246_M	250_M	0.582	0.62
45_H	55_V	0.581	0.61
108_G	112_V	0.580	0.61
256_S	263_L	0.578	0.61
107_A	280_G	0.578	0.61
28_F	304_F	0.577	0.61
176_L	210_A	0.577	0.61
23_K	301_N	0.576	0.61
80_M	84_A	0.573	0.60
68_P	100_N	0.571	0.60
312_E	315_S	0.570	0.60
287_L	290_P	0.569	0.60
64_P	210_A	0.568	0.60
90_N	288_K	0.563	0.59
177_N	297_S	0.561	0.59
5_V	307_I	0.559	0.58
245_M	248_D	0.559	0.58
278_N	305_H	0.558	0.58
160_V	163_Q	0.557	0.58
155_A	192_T	0.556	0.58
75_K	81_V	0.555	0.58
308_R	311_R	0.554	0.58
262_V	265_P	0.554	0.58
287_L	298_S	0.554	0.58
143_N	146_A	0.554	0.58
94_A	314_V	0.554	0.58
223_T	238_S	0.553	0.58
168_V	171_P	0.552	0.58
69_V	207_N	0.551	0.57
88_A	112_V	0.551	0.57
4_A	95_C	0.550	0.57
123_L	140_V	0.548	0.57
101_T	104_L	0.548	0.57
15_K	48_T	0.546	0.57
4_A	289_A	0.545	0.57
292_I	313_M	0.545	0.57
36_V	83_M	0.544	0.56
247_R	257_K	0.541	0.56
28_F	315_S	0.540	0.56
160_V	322_I	0.540	0.56
39_K	55_V	0.540	0.56
276_Y	298_S	0.539	0.56
172_R	203_N	0.537	0.55
156_I	159_S	0.535	0.55
252_S	269_E	0.533	0.55
244_K	248_D	0.533	0.55
39_K	59_D	0.532	0.55
251_T	254_L	0.530	0.55
301_N	305_H	0.530	0.55
260_A	263_L	0.529	0.54
43_E	48_T	0.528	0.54
64_P	180_T	0.528	0.54
32_H	51_D	0.526	0.54
84_A	87_V	0.526	0.54
64_P	100_N	0.525	0.54
323_Q	327_K	0.525	0.54
75_K	107_A	0.524	0.54
61_V	64_P	0.524	0.54
258_L	263_L	0.524	0.54
173_V	222_V	0.524	0.54
239_A	243_F	0.521	0.53
316_Q	326_V	0.518	0.53
71_A	106_T	0.517	0.53
25_I	50_S	0.517	0.53
105_M	109_L	0.517	0.53
256_S	260_A	0.516	0.53
126_T	135_F	0.516	0.53
215_D	241_S	0.515	0.52
62_I	107_A	0.514	0.52
177_N	303_V	0.514	0.52
30_D	52_R	0.513	0.52
243_F	271_K	0.512	0.52
292_I	309_Q	0.510	0.52
40_T	43_E	0.510	0.52
135_F	158_G	0.510	0.52
239_A	242_I	0.510	0.52
39_K	58_A	0.508	0.51
259_A	263_L	0.508	0.51
302_A	306_A	0.508	0.51
112_V	288_K	0.508	0.51
93_D	314_V	0.507	0.51
129_T	235_L	0.507	0.51
69_V	72_V	0.506	0.51
115_I	326_V	0.506	0.51
44_S	48_T	0.505	0.51
98_A	294_A	0.504	0.51
131_S	217_V	0.503	0.51
211_R	215_D	0.501	0.51
256_S	269_E	0.501	0.51
2_R	54_T	0.500	0.50

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1u7nA	2	0.961	100	0.346	Contact Map
1vi1A	2	0.985	100	0.35	Contact Map
1vmiA	2	0.8108	100	0.58	Contact Map
2af4C	2	0.8318	100	0.583	Contact Map
1td9A	3	0.8138	100	0.605	Contact Map
1r5jA	4	0.8228	100	0.609	Contact Map
3uf6A	2	0.7477	100	0.62	Contact Map
4e4rA	2	0.8138	100	0.621	Contact Map
1ycoA	2	0.7327	100	0.638	Contact Map
3lxyA	2	0.8318	100	0.692	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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