GREMLIN Database
SPEE - Polyamine aminopropyl transferase
UniProt: P70998 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 276 (241)
Sequences: 3290 (2135)
Seq/√Len: 137.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
78_E101_K4.9921.00
177_A236_K4.1301.00
104_T150_Y3.5491.00
22_K38_E3.4431.00
27_E111_K3.3411.00
110_G114_E2.9991.00
79_H102_K2.9171.00
104_T135_Q2.9061.00
82_V91_I2.8381.00
102_K133_D2.7661.00
113_I136_V2.7371.00
83_V139_G2.5951.00
26_T33_H2.5431.00
176_I186_F2.4941.00
141_M145_K2.4571.00
36_M115_Y2.4041.00
170_K174_A2.3971.00
170_K209_E2.3701.00
38_E44_N2.2921.00
91_I132_V2.2921.00
137_D145_K2.2801.00
21_N38_E2.2541.00
180_L184_G2.1721.00
212_P236_K2.1491.00
144_A175_G2.1311.00
104_T133_D2.1181.00
25_H111_K2.0131.00
28_Q33_H2.0071.00
153_I180_L2.0001.00
183_D237_K1.9971.00
91_I103_A1.9841.00
82_V94_I1.9751.00
147_E178_K1.9721.00
114_E118_K1.9701.00
143_I175_G1.9581.00
111_K114_E1.9571.00
110_G136_V1.9461.00
21_N40_E1.9111.00
29_T111_K1.8401.00
143_I179_A1.8161.00
81_L150_Y1.8141.00
153_I176_I1.7881.00
146_S179_A1.7691.00
117_K128_D1.7591.00
74_H77_P1.7471.00
79_H181_K1.7411.00
95_L103_A1.7211.00
29_T34_L1.6961.00
56_E122_S1.6821.00
80_V94_I1.6381.00
91_I127_L1.6131.00
153_I179_A1.6011.00
190_T232_T1.5991.00
26_T35_E1.5991.00
54_T87_D1.5811.00
103_A132_V1.5791.00
146_S149_Q1.5711.00
79_H148_N1.5541.00
94_I100_V1.5381.00
137_D141_M1.4941.00
140_F166_N1.4801.00
70_P152_V1.4341.00
135_Q142_H1.4181.00
71_L152_V1.3991.00
207_V234_G1.3931.00
106_V142_H1.3781.00
25_H115_Y1.3551.00
155_V186_F1.3471.00
80_V100_V1.3311.00
155_V172_F1.3211.00
19_K40_E1.3161.00
141_M144_A1.3151.00
77_P99_S1.3101.00
36_M46_L1.3081.00
183_D236_K1.3011.00
207_V232_T1.2891.00
79_H149_Q1.2711.00
30_E110_G1.2631.00
81_L106_V1.2631.00
68_H97_H1.2631.00
182_E236_K1.2221.00
83_V157_S1.2171.00
139_G157_S1.2151.00
142_H150_Y1.1951.00
221_I224_Y1.1821.00
175_G179_A1.1620.99
143_I176_I1.1590.99
183_D238_Y1.1540.99
142_H146_S1.1490.99
37_V47_F1.1480.99
165_V169_T1.1190.99
171_G174_A1.0790.99
67_A154_M1.0540.99
105_L134_V1.0520.99
67_A90_V1.0470.99
74_H152_V1.0440.99
125_G128_D1.0430.99
140_F175_G1.0330.99
113_I134_V1.0270.99
182_E237_K1.0260.99
188_A207_V1.0190.99
246_S249_F1.0180.99
204_Q208_K1.0160.98
147_E179_A1.0150.98
140_F171_G1.0100.98
23_T35_E1.0090.98
149_Q181_K1.0070.98
93_E96_K1.0040.98
152_V185_I0.9990.98
81_L153_I0.9890.98
246_S250_D0.9850.98
95_L132_V0.9700.98
71_L94_I0.9580.98
213_I238_Y0.9520.98
80_V152_V0.9450.98
155_V188_A0.9370.97
108_I138_D0.9360.97
85_G105_L0.9320.97
111_K115_Y0.9220.97
213_I235_S0.9200.97
29_T109_D0.9150.97
155_V176_I0.9100.97
127_L132_V0.9100.97
204_Q214_T0.9090.97
71_L97_H0.9090.97
24_L119_F0.9020.97
137_D142_H0.8980.97
54_T123_I0.8940.97
204_Q232_T0.8910.97
95_L126_K0.8890.96
34_L115_Y0.8860.96
235_S239_D0.8790.96
219_A231_F0.8710.96
153_I184_G0.8690.96
56_E120_L0.8610.96
94_I103_A0.8580.96
151_D181_K0.8540.96
95_L131_R0.8500.95
88_G113_I0.8450.95
203_V206_D0.8440.95
81_L142_H0.8400.95
65_M69_V0.8380.95
177_A212_P0.8370.95
79_H104_T0.8340.95
61_V219_A0.8340.95
148_N182_E0.8330.95
146_S150_Y0.8230.95
36_M120_L0.8220.95
242_A245_D0.8200.95
91_I105_L0.8180.94
218_T228_L0.8180.94
200_I216_L0.8170.94
63_H90_V0.8140.94
241_L244_E0.8110.94
245_D250_D0.8040.94
169_T206_D0.7980.94
53_M62_Y0.7960.94
129_D132_V0.7930.93
186_F234_G0.7900.93
69_V217_Y0.7870.93
214_T234_G0.7870.93
74_H185_I0.7870.93
105_L132_V0.7840.93
32_Q109_D0.7840.93
175_G178_K0.7820.93
216_L230_T0.7750.93
201_T205_R0.7730.93
83_V155_V0.7710.92
70_P185_I0.7710.92
244_E248_F0.7700.92
174_A210_I0.7660.92
174_A178_K0.7650.92
66_V187_V0.7630.92
94_I152_V0.7590.92
172_F176_I0.7580.92
148_N179_A0.7560.92
240_P244_E0.7560.92
54_T120_L0.7550.92
39_T45_M0.7520.92
58_D223_T0.7510.91
148_N181_K0.7500.91
66_V90_V0.7480.91
246_S251_I0.7480.91
72_F97_H0.7460.91
60_F254_K0.7430.91
80_V154_M0.7430.91
70_P217_Y0.7390.91
23_T26_T0.7360.91
251_I254_K0.7360.91
81_L143_I0.7360.91
62_Y156_D0.7340.91
39_T43_G0.7340.91
82_V132_V0.7330.90
67_A71_L0.7320.90
25_H34_L0.7300.90
247_R251_I0.7300.90
178_K182_E0.7250.90
116_S119_F0.7220.90
245_D249_F0.7210.90
243_V247_R0.7210.90
221_I225_P0.7200.90
188_A232_T0.7190.90
59_E92_R0.7180.90
120_L123_I0.7170.90
21_N39_T0.7170.90
82_V85_G0.7130.89
170_K210_I0.7090.89
248_F251_I0.7080.89
15_G19_K0.7080.89
85_G109_D0.7070.89
114_E117_K0.7040.89
71_L100_V0.7020.89
45_M52_V0.7020.89
47_F52_V0.7020.89
213_I239_D0.7020.89
160_P168_F0.6980.88
202_N206_D0.6980.88
139_G155_V0.6970.88
162_G165_V0.6960.88
176_I206_D0.6950.88
170_K206_D0.6940.88
106_V137_D0.6930.88
18_M45_M0.6920.88
65_M217_Y0.6910.88
78_E181_K0.6900.88
215_K239_D0.6870.87
248_F252_E0.6850.87
67_A93_E0.6840.87
245_D248_F0.6830.87
23_T37_V0.6830.87
37_V45_M0.6820.87
249_F253_T0.6810.87
60_F63_H0.6790.87
158_T189_Q0.6740.86
64_E93_E0.6730.86
206_D209_E0.6700.86
68_H93_E0.6690.86
91_I94_I0.6690.86
63_H66_V0.6660.86
74_H78_E0.6630.86
94_I154_M0.6570.85
30_E111_K0.6560.85
144_A171_G0.6550.85
51_M161_V0.6550.85
95_L129_D0.6550.85
239_D242_A0.6540.85
27_E114_E0.6530.85
63_H67_A0.6450.84
247_R250_D0.6450.84
30_E34_L0.6420.84
88_G190_T0.6420.84
187_V217_Y0.6410.84
67_A70_P0.6390.83
91_I126_K0.6390.83
190_T230_T0.6380.83
189_Q229_W0.6370.83
149_Q179_A0.6360.83
204_Q216_L0.6350.83
91_I191_D0.6320.83
64_E68_H0.6310.83
121_P125_G0.6290.82
76_N183_D0.6280.82
220_N228_L0.6260.82
65_M219_A0.6240.82
59_E62_Y0.6230.82
220_N224_Y0.6210.82
88_G127_L0.6200.82
219_A222_P0.6160.81
43_G56_E0.6150.81
174_A177_A0.6130.81
53_M63_H0.6130.81
180_L186_F0.6070.80
66_V230_T0.6070.80
54_T107_D0.6060.80
173_Y203_V0.6060.80
29_T85_G0.6050.80
15_G18_M0.6050.80
202_N205_R0.6040.80
100_V103_A0.6030.80
244_E249_F0.6000.79
85_G88_G0.5990.79
241_L245_D0.5990.79
27_E30_E0.5940.79
127_L134_V0.5940.79
70_P187_V0.5900.78
90_V154_M0.5870.78
77_P185_I0.5870.78
87_D107_D0.5830.77
79_H101_K0.5800.77
108_I139_G0.5800.77
69_V233_I0.5780.77
156_D189_Q0.5760.77
71_L80_V0.5750.76
213_I237_K0.5720.76
143_I172_F0.5720.76
200_I230_T0.5710.76
51_M112_V0.5690.76
87_D90_V0.5670.75
122_S125_G0.5620.75
29_T63_H0.5570.74
139_G167_L0.5570.74
147_E182_E0.5570.74
161_V169_T0.5560.74
24_L38_E0.5540.74
29_T32_Q0.5530.74
146_S209_E0.5520.74
63_H69_V0.5510.73
105_L113_I0.5510.73
216_L232_T0.5510.73
219_A255_Y0.5510.73
126_K129_D0.5490.73
116_S120_L0.5480.73
60_F64_E0.5470.73
76_N99_S0.5470.73
53_M191_D0.5470.73
86_G112_V0.5460.73
203_V207_V0.5460.73
70_P73_T0.5450.73
36_M44_N0.5450.73
55_S58_D0.5420.72
176_I184_G0.5410.72
24_L44_N0.5400.72
46_L62_Y0.5400.72
215_K241_L0.5380.72
65_M68_H0.5370.72
54_T89_G0.5370.72
187_V233_I0.5360.71
87_D105_L0.5350.71
85_G93_E0.5350.71
244_E252_E0.5320.71
53_M155_V0.5310.71
222_P225_P0.5310.71
240_P243_V0.5290.70
158_T168_F0.5280.70
87_D189_Q0.5280.70
97_H100_V0.5270.70
214_T232_T0.5260.70
84_G156_D0.5250.70
116_S121_P0.5250.70
59_E63_H0.5240.70
100_V131_R0.5230.70
34_L111_K0.5200.69
158_T229_W0.5180.69
145_K149_Q0.5170.69
29_T112_V0.5160.69
46_L112_V0.5150.68
24_L112_V0.5140.68
155_V159_E0.5140.68
53_M189_Q0.5110.68
193_P229_W0.5100.68
35_E47_F0.5100.68
168_F189_Q0.5090.67
186_F207_V0.5060.67
102_K135_Q0.5060.67
159_E189_Q0.5060.67
220_N223_T0.5060.67
19_K41_E0.5050.67
44_N122_S0.5020.66
108_I161_V0.5020.66
100_V152_V0.5020.66
249_F252_E0.5020.66
107_D205_R0.5000.66
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1inlA 3 0.9855 100 0.326 Contact Map
2o07A 2 1 100 0.339 Contact Map
1xj5A 4 1 100 0.349 Contact Map
2pt6A 2 0.9928 100 0.35 Contact Map
2i7cA 2 0.9928 100 0.351 Contact Map
1iy9A 3 0.9928 100 0.351 Contact Map
1uirA 4 0.9964 100 0.351 Contact Map
2b2cA 2 0.9167 100 0.355 Contact Map
1mjfA 2 0.9529 100 0.358 Contact Map
2cmgA 2 0.9239 100 0.362 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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