GREMLIN Database
NPRE - Bacillolysin
UniProt: P68736 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 521 (453)
Sequences: 1694 (1225)
Seq/√Len: 57.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
371_T391_S5.3031.00
470_K508_D5.2231.00
186_A213_L3.9271.00
483_L487_S3.9041.00
386_L492_A3.8751.00
305_Y310_F3.7001.00
497_I509_A3.6951.00
483_L495_A3.6901.00
384_G448_V3.6751.00
173_L185_L3.4581.00
92_H112_H3.3001.00
262_T296_H3.2591.00
306_F367_T3.1881.00
375_A479_L3.0631.00
230_T293_V3.0071.00
389_S455_P2.9801.00
397_F472_Q2.9431.00
114_D118_N2.8241.00
482_Y502_D2.8221.00
378_I486_S2.7581.00
396_Y405_I2.7341.00
310_F473_Q2.7161.00
470_K504_Y2.6141.00
184_R206_D2.5771.00
138_Q185_L2.5681.00
103_P172_E2.4901.00
305_Y472_Q2.4841.00
327_H346_D2.4761.00
488_T491_D2.4711.00
496_L516_W2.4371.00
454_I519_V2.4001.00
385_A451_N2.3591.00
363_A395_G2.3341.00
443_G454_I2.3051.00
421_N424_K2.3041.00
172_E188_D2.2621.00
315_Y369_G2.2571.00
108_Q485_P2.2541.00
205_V212_I2.2281.00
332_Y336_A2.1721.00
393_V475_Y2.0991.00
324_S344_Y2.0891.00
141_S144_K2.0851.00
200_N338_T2.0841.00
444_D447_G2.0641.00
361_V409_I2.0581.00
328_Y332_Y2.0451.00
477_R502_D2.0401.00
429_D443_G2.0251.00
320_S369_G2.0141.00
299_L324_S2.0141.00
251_D254_K2.0041.00
459_A471_S1.9731.00
497_I513_E1.9151.00
498_Q502_D1.9031.00
188_D202_E1.8721.00
336_A343_I1.8691.00
281_T284_F1.8631.00
80_L95_Y1.8350.99
279_S284_F1.8250.99
339_G372_Q1.8200.99
145_A149_A1.8150.99
230_T233_G1.8100.99
295_A326_V1.8070.99
477_R481_T1.8060.99
306_F370_V1.8040.99
189_V205_V1.8010.99
372_Q376_N1.7900.99
85_T91_K1.7520.99
111_V119_V1.7410.99
112_H482_Y1.7330.99
75_S100_N1.7210.99
202_E218_K1.7140.99
325_S343_I1.6910.99
325_S341_Q1.6820.99
379_Y452_S1.6790.99
382_Q431_Y1.6710.99
337_W372_Q1.6660.99
383_P448_V1.6590.99
240_I282_K1.6560.99
86_D92_H1.6550.99
176_I186_A1.6410.99
504_Y508_D1.6230.99
446_G451_N1.6030.99
315_Y320_S1.5910.99
135_D208_E1.5830.98
140_V187_Y1.5790.98
240_I284_F1.5640.98
206_D209_T1.5420.98
243_E246_K1.5360.98
199_A217_N1.5340.98
80_L93_F1.5270.98
462_T512_V1.4950.98
302_V306_F1.4720.98
455_P516_W1.4650.97
86_D477_R1.4590.97
310_F476_Y1.4530.97
501_R509_A1.4510.97
425_Y428_P1.4480.97
420_S424_K1.4150.97
279_S282_K1.4100.97
279_S289_Q1.4100.97
333_N348_D1.4060.97
447_G451_N1.4040.97
418_S424_K1.4030.97
465_K518_A1.4020.97
394_F479_L1.3890.96
112_H123_N1.3840.96
78_L95_Y1.3810.96
446_G454_I1.3710.96
263_Y276_L1.3710.96
214_K380_E1.3610.96
399_D402_D1.3610.96
389_S393_V1.3560.96
295_A324_S1.3560.96
206_D213_L1.3510.96
305_Y309_N1.3500.96
509_A513_E1.3480.96
482_Y499_S1.3390.96
507_T511_K1.3390.96
105_K204_L1.3310.95
516_W521_L1.3300.95
152_A203_V1.3220.95
453_G456_N1.3190.95
242_Y245_G1.2970.95
264_D288_S1.2920.95
374_T480_T1.2890.95
418_S425_Y1.2890.95
393_V459_A1.2830.94
138_Q173_L1.2820.94
333_N352_F1.2760.94
139_K208_E1.2690.94
510_A514_A1.2670.94
477_R503_L1.2660.94
191_I203_V1.2630.94
279_S283_T1.2600.94
292_A326_V1.2590.94
487_S491_D1.2570.94
455_P489_F1.2550.94
242_Y246_K1.2480.94
185_L207_A1.2440.94
332_Y346_D1.2440.94
466_L470_K1.2430.93
247_Y251_D1.2370.93
510_A513_E1.2370.93
458_A512_V1.2370.93
303_Y314_S1.2270.93
367_T394_F1.2160.93
431_Y434_Y1.2100.93
93_F113_V1.2020.92
226_G230_T1.2020.92
404_D424_K1.2010.92
190_T202_E1.1970.92
452_S456_N1.1920.92
410_T414_P1.1820.92
190_T200_N1.1820.92
394_F472_Q1.1810.92
371_T479_L1.1810.92
500_A508_D1.1780.92
429_D433_N1.1750.91
306_F394_F1.1630.91
310_F472_Q1.1570.91
241_S250_R1.1520.91
478_A483_L1.1520.91
230_T234_A1.1510.91
334_N448_V1.1470.90
382_Q448_V1.1460.90
393_V455_P1.1460.90
365_E368_H1.1390.90
304_D308_S1.1390.90
447_G450_T1.1390.90
296_H300_G1.1360.90
60_K95_Y1.1300.90
458_A462_T1.1270.90
393_V422_P1.1260.90
382_Q434_Y1.1130.89
299_L344_Y1.1120.89
313_N317_N1.1000.88
229_T235_T1.1000.88
152_A215_Q1.0950.88
114_D120_Y1.0950.88
173_L207_A1.0870.88
387_N391_S1.0800.88
375_A483_L1.0800.88
328_Y336_A1.0760.87
291_A354_P1.0670.87
429_D446_G1.0660.87
478_A496_L1.0630.87
512_V521_L1.0610.87
376_N485_P1.0610.87
94_R110_I1.0600.87
396_Y402_D1.0540.86
269_Q328_Y1.0520.86
363_A396_Y1.0480.86
230_T236_V1.0420.86
493_K517_N1.0390.85
342_M366_M1.0390.85
230_T294_D1.0380.85
140_V148_L1.0330.85
228_G293_V1.0330.85
482_Y498_Q1.0320.85
443_G446_G1.0290.85
401_E468_V1.0290.85
429_D435_R1.0240.85
112_H502_D1.0240.85
427_Q453_G1.0240.85
362_T366_M1.0220.84
148_L151_K1.0210.84
482_Y495_A1.0190.84
102_V185_L1.0160.84
62_F66_N1.0150.84
461_N519_V1.0130.84
140_V144_K1.0130.84
423_T433_N1.0120.84
247_Y254_K1.0110.84
238_L249_L1.0080.84
334_N449_H1.0040.83
241_S248_V1.0040.83
144_K147_A0.9990.83
328_Y343_I0.9970.83
432_A435_R0.9930.83
379_Y448_V0.9820.82
212_I215_Q0.9810.82
369_G373_E0.9770.82
88_L477_R0.9750.82
496_L512_V0.9730.81
313_N319_G0.9730.81
109_V122_V0.9710.81
500_A506_S0.9670.81
335_A406_G0.9660.81
79_K98_V0.9650.81
444_D451_N0.9650.81
379_Y383_P0.9600.81
342_M355_L0.9550.80
248_V278_S0.9530.80
461_N518_A0.9520.80
477_R499_S0.9510.80
387_N452_S0.9510.80
390_F475_Y0.9480.80
422_P519_V0.9470.80
513_E517_N0.9460.80
130_S134_T0.9440.79
397_F468_V0.9430.79
316_D320_S0.9430.79
405_I416_L0.9420.79
171_A189_V0.9390.79
457_K461_N0.9390.79
102_V176_I0.9380.79
498_Q501_R0.9360.79
312_R476_Y0.9340.79
379_Y456_N0.9330.79
494_A498_Q0.9280.78
386_L489_F0.9230.78
169_N173_L0.9200.78
243_E248_V0.9180.77
514_A517_N0.9180.77
325_S328_Y0.9170.77
238_L242_Y0.9120.77
144_K148_L0.9120.77
384_G452_S0.9060.77
269_Q329_G0.9060.77
305_Y473_Q0.9030.76
309_N473_Q0.9030.76
53_A61_Q0.9020.76
371_T387_N0.8990.76
361_V416_L0.8940.76
385_A446_G0.8910.75
466_L508_D0.8910.75
52_S56_D0.8890.75
377_L383_P0.8880.75
379_Y384_G0.8850.75
305_Y394_F0.8840.75
405_I419_L0.8810.74
229_T233_G0.8790.74
99_V104_I0.8780.74
54_P68_N0.8770.74
423_T457_K0.8760.74
478_A495_A0.8740.74
324_S362_T0.8740.74
379_Y447_G0.8720.74
404_D418_S0.8710.74
226_G229_T0.8710.74
264_D267_N0.8710.74
406_G409_I0.8680.73
384_G406_G0.8670.73
464_T468_V0.8670.73
291_A294_D0.8630.73
204_L216_Q0.8630.73
219_V338_T0.8600.73
316_D373_E0.8590.73
298_N362_T0.8560.72
404_D407_E0.8560.72
227_S230_T0.8540.72
433_N443_G0.8540.72
459_A463_I0.8520.72
148_L205_V0.8470.72
241_S252_L0.8470.72
474_I508_D0.8460.71
411_V414_P0.8450.71
200_N218_K0.8440.71
419_L456_N0.8440.71
313_N316_D0.8430.71
277_V281_T0.8430.71
105_K378_I0.8420.71
446_G450_T0.8350.70
385_A447_G0.8340.70
508_D511_K0.8310.70
57_K61_Q0.8280.70
309_N317_N0.8270.70
454_I458_A0.8260.70
430_N434_Y0.8220.69
255_P259_Q0.8210.69
406_G448_V0.8200.69
470_K474_I0.8190.69
124_G520_G0.8180.69
415_A418_S0.8150.69
337_W369_G0.8150.69
324_S366_M0.8150.69
384_G387_N0.8110.68
227_S235_T0.8090.68
249_L254_K0.8080.68
240_I279_S0.8080.68
309_N469_S0.8060.68
175_A178_T0.8040.68
493_K497_I0.8010.67
444_D448_V0.8010.67
370_V394_F0.7980.67
333_N355_L0.7960.67
434_Y454_I0.7940.67
317_N476_Y0.7930.66
358_S407_E0.7910.66
200_N372_Q0.7900.66
435_R446_G0.7900.66
433_N454_I0.7890.66
267_N288_S0.7890.66
454_I489_F0.7870.66
397_F459_A0.7850.66
45_A48_Q0.7830.65
348_D409_I0.7830.65
352_F416_L0.7820.65
372_Q387_N0.7790.65
50_E54_P0.7790.65
54_P57_K0.7770.65
386_L487_S0.7750.65
491_D494_A0.7750.65
429_D432_A0.7750.65
153_I191_I0.7740.65
469_S472_Q0.7720.64
393_V460_Y0.7720.64
455_P496_L0.7690.64
51_L58_A0.7680.64
336_A416_L0.7670.64
389_S516_W0.7670.64
254_K259_Q0.7630.63
149_A203_V0.7590.63
245_G248_V0.7580.63
404_D420_S0.7560.63
345_G355_L0.7550.63
461_N464_T0.7540.63
404_D415_A0.7510.62
77_R98_V0.7510.62
377_L452_S0.7500.62
273_P276_L0.7470.62
56_D60_K0.7460.62
423_T426_N0.7430.61
333_N349_G0.7360.61
176_I213_L0.7360.61
332_Y343_I0.7320.60
367_T371_T0.7320.60
262_T299_L0.7310.60
348_D414_P0.7300.60
148_L152_A0.7300.60
461_N465_K0.7290.60
428_P450_T0.7290.60
50_E61_Q0.7290.60
374_T479_L0.7290.60
147_A151_K0.7290.60
53_A57_K0.7280.60
119_V122_V0.7270.60
114_D129_Q0.7240.59
262_T265_L0.7240.59
332_Y355_L0.7230.59
206_D210_G0.7210.59
355_L416_L0.7200.59
126_L130_S0.7190.59
511_K515_A0.7190.59
458_A519_V0.7190.59
146_L169_N0.7140.58
247_Y253_S0.7130.58
272_L277_V0.7120.58
335_A449_H0.7090.58
221_H405_I0.7070.58
462_T471_S0.7070.58
422_P453_G0.7060.57
230_T290_R0.7030.57
239_N253_S0.7020.57
429_D454_I0.7010.57
200_N339_G0.7010.57
473_Q480_T0.6970.57
388_E392_D0.6970.57
149_A191_I0.6950.56
178_T184_R0.6940.56
499_S502_D0.6940.56
428_P451_N0.6930.56
81_V96_A0.6930.56
110_I484_T0.6920.56
134_T146_L0.6920.56
475_Y515_A0.6910.56
176_I184_R0.6910.56
240_I255_P0.6890.56
266_Q273_P0.6890.56
101_G339_G0.6880.56
48_Q54_P0.6880.56
469_S473_Q0.6860.55
326_V356_S0.6850.55
397_F463_I0.6840.55
394_F476_Y0.6820.55
140_V210_G0.6810.55
474_I500_A0.6780.54
458_A475_Y0.6780.54
308_S473_Q0.6770.54
50_E53_A0.6770.54
422_P457_K0.6760.54
200_N219_V0.6750.54
297_Y398_N0.6750.54
99_V134_T0.6750.54
497_I512_V0.6750.54
355_L361_V0.6740.54
55_N58_A0.6730.54
228_G232_K0.6710.54
317_N466_L0.6710.54
355_L409_I0.6700.54
359_L415_A0.6700.54
422_P460_Y0.6690.53
315_Y373_E0.6680.53
291_A356_S0.6670.53
100_N136_N0.6670.53
393_V472_Q0.6670.53
306_F476_Y0.6650.53
299_L356_S0.6650.53
389_S456_N0.6650.53
278_S284_F0.6640.53
59_V111_V0.6640.53
236_V298_N0.6640.53
474_I512_V0.6630.53
229_T293_V0.6610.53
423_T430_N0.6590.52
140_V145_A0.6580.52
387_N479_L0.6560.52
184_R211_S0.6550.52
48_Q58_A0.6550.52
156_S184_R0.6550.52
333_N410_T0.6540.52
48_Q51_L0.6530.52
143_E146_L0.6530.52
277_V288_S0.6530.52
285_T289_Q0.6530.52
483_L496_L0.6520.52
410_T415_A0.6520.52
50_E58_A0.6510.51
390_F492_A0.6510.51
342_M369_G0.6480.51
317_N477_R0.6470.51
459_A519_V0.6460.51
289_Q293_V0.6460.51
434_Y445_Y0.6460.51
460_Y463_I0.6420.51
278_S283_T0.6420.51
474_I496_L0.6420.51
430_N520_G0.6420.51
49_S52_S0.6410.50
443_G519_V0.6400.50
379_Y391_S0.6390.50
269_Q332_Y0.6360.50
264_D277_V0.6340.50
175_A186_A0.6340.50
364_H368_H0.6330.50
45_A50_E0.6320.49
274_G277_V0.6310.49
73_D76_K0.6300.49
396_Y407_E0.6290.49
97_P369_G0.6270.49
173_L187_Y0.6270.49
445_Y451_N0.6260.49
382_Q465_K0.6240.49
429_D434_Y0.6240.49
246_K250_R0.6240.49
314_S320_S0.6230.48
241_S246_K0.6210.48
167_N191_I0.6200.48
371_T374_T0.6200.48
67_S126_L0.6200.48
110_I481_T0.6200.48
422_P459_A0.6190.48
516_W519_V0.6180.48
364_H392_D0.6180.48
367_T390_F0.6170.48
330_T336_A0.6170.48
69_I405_I0.6170.48
111_V145_A0.6160.48
435_R444_D0.6150.48
430_N433_N0.6150.48
301_K358_S0.6120.47
475_Y496_L0.6110.47
136_N185_L0.6100.47
141_S148_L0.6090.47
309_N397_F0.6080.47
333_N356_S0.6080.47
333_N409_I0.6060.47
106_D415_A0.6060.47
139_K144_K0.6040.46
254_K304_D0.6030.46
376_N514_A0.6020.46
349_G414_P0.6020.46
185_L210_G0.6010.46
72_G77_R0.6010.46
245_G253_S0.6000.46
168_S171_A0.6000.46
138_Q142_S0.5990.46
277_V284_F0.5990.46
240_I247_Y0.5980.46
471_S516_W0.5980.46
338_T341_Q0.5970.46
295_A356_S0.5960.45
405_I468_V0.5960.45
327_H347_G0.5940.45
141_S151_K0.5930.45
477_R482_Y0.5910.45
455_P492_A0.5900.45
403_W464_T0.5890.45
305_Y469_S0.5890.45
358_S371_T0.5890.45
70_F189_V0.5890.45
365_E388_E0.5880.45
76_K205_V0.5860.44
382_Q455_P0.5860.44
201_W215_Q0.5860.44
384_G447_G0.5860.44
368_H388_E0.5850.44
249_L253_S0.5840.44
74_P79_K0.5840.44
129_Q133_K0.5830.44
54_P58_A0.5830.44
462_T511_K0.5830.44
386_L485_P0.5820.44
225_T230_T0.5820.44
99_V102_V0.5810.44
299_L370_V0.5810.44
352_F410_T0.5800.44
100_N185_L0.5800.44
66_N70_F0.5800.44
263_Y275_T0.5780.43
379_Y451_N0.5780.43
389_S452_S0.5770.43
273_P277_V0.5760.43
52_S61_Q0.5760.43
299_L366_M0.5750.43
367_T370_V0.5720.43
46_I51_L0.5720.43
388_E449_H0.5710.43
349_G352_F0.5710.43
403_W422_P0.5710.43
431_Y520_G0.5700.43
393_V465_K0.5690.43
192_R200_N0.5670.42
171_A499_S0.5670.42
377_L387_N0.5650.42
238_L243_E0.5650.42
241_S244_G0.5650.42
225_T229_T0.5640.42
421_N427_Q0.5640.42
478_A487_S0.5640.42
84_T481_T0.5630.42
51_L55_N0.5630.42
353_S410_T0.5610.42
48_Q53_A0.5600.42
342_M391_S0.5600.42
396_Y401_E0.5600.42
296_H324_S0.5590.41
419_L428_P0.5570.41
493_K516_W0.5550.41
86_D306_F0.5540.41
246_K249_L0.5540.41
47_A50_E0.5530.41
366_M369_G0.5530.41
391_S479_L0.5520.41
135_D185_L0.5520.41
333_N411_V0.5510.41
99_V130_S0.5470.40
201_W217_N0.5470.40
78_L204_L0.5460.40
80_L113_V0.5460.40
390_F393_V0.5450.40
314_S318_K0.5450.40
312_R374_T0.5440.40
291_A346_D0.5430.40
144_K151_K0.5420.40
229_T232_K0.5410.40
500_A512_V0.5410.40
459_A462_T0.5400.39
138_Q208_E0.5400.39
320_S373_E0.5400.39
459_A472_Q0.5380.39
75_S147_A0.5380.39
379_Y446_G0.5370.39
315_Y391_S0.5360.39
298_N359_L0.5360.39
365_E392_D0.5360.39
435_R445_Y0.5360.39
65_K182_S0.5360.39
114_D137_S0.5340.39
266_Q275_T0.5340.39
309_N476_Y0.5340.39
335_A417_R0.5340.39
105_K188_D0.5340.39
465_K514_A0.5330.39
78_L97_P0.5320.39
378_I383_P0.5320.39
510_A517_N0.5320.39
458_A489_F0.5320.39
368_H392_D0.5320.39
386_L516_W0.5320.39
431_Y521_L0.5310.39
375_A485_P0.5300.38
57_K60_K0.5300.38
343_I390_F0.5300.38
250_R261_I0.5300.38
458_A496_L0.5290.38
392_D417_R0.5280.38
345_G453_G0.5280.38
337_W344_Y0.5280.38
263_Y323_V0.5270.38
265_L273_P0.5260.38
342_M371_T0.5240.38
466_L511_K0.5220.38
99_V183_Y0.5210.37
421_N430_N0.5210.37
59_V80_L0.5210.37
102_V181_G0.5200.37
295_A299_L0.5200.37
188_D204_L0.5190.37
310_F480_T0.5190.37
434_Y444_D0.5180.37
145_A210_G0.5180.37
434_Y489_F0.5180.37
418_S451_N0.5160.37
79_K119_V0.5150.37
109_V124_G0.5150.37
96_A108_Q0.5140.37
192_R217_N0.5140.37
86_D112_H0.5130.37
334_N384_G0.5130.37
499_S503_L0.5120.37
146_L171_A0.5120.37
344_Y366_M0.5120.37
238_L241_S0.5110.36
49_S54_P0.5110.36
267_N285_T0.5110.36
271_R277_V0.5100.36
336_A355_L0.5100.36
46_I57_K0.5100.36
379_Y389_S0.5090.36
379_Y450_T0.5090.36
85_T140_V0.5080.36
195_E217_N0.5070.36
166_K169_N0.5070.36
322_I341_Q0.5060.36
255_P321_K0.5060.36
52_S57_K0.5060.36
92_H395_G0.5050.36
371_T456_N0.5050.36
95_Y111_V0.5040.36
456_N461_N0.5040.36
264_D268_R0.5030.36
455_P458_A0.5030.36
453_G457_K0.5020.36
274_G325_S0.5020.36
309_N472_Q0.5010.35
63_L76_K0.5000.35
125_E189_V0.5000.35
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1bqbA 1 0.5605 100 0.407 Contact Map
4n4eE 1 0.5662 100 0.411 Contact Map
4gerA 1 0.5528 100 0.414 Contact Map
3nqxA 1 0.5355 100 0.469 Contact Map
4k89A 1 0.5432 100 0.476 Contact Map
2vqxA 1 0.5681 100 0.483 Contact Map
4k90A 1 0.595 100 0.498 Contact Map
3nqzA 1 0.334 100 0.775 Contact Map
4k90B 1 0.3608 99.9 0.805 Contact Map
2o3oA 2 0.3743 98.4 0.921 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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