YBAS - BACSU - GREMLIN Contacts

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YBAS - Uncharacterized protein YbaS

UniProt:	P55190	Sequences: MPLLTPTSVVLGVLLSQFLNGYEWAVPWIFAFITFAGSLSANFQSLRHALSHPLPMILALFVLHIFMPLFAWGSGHLIFKGDPLTITGLTLAVVIPTGITSLIWAAMYKGNVGLTLSIILVDTVLSPLIVPLSLSLLAGAQVHMDVWGMMKGLIVMVVIPSFLGMLFNQMSSPERTAFVSSALSPFSKLCLMAVIAINSSAIAPYFKSIDLRFAGIAVTVFFIALTGYAAAWLIGKMMKRRQEEIVSLIFTGGMRNISAGAVLAVTFFPSQVAVPVVIGMLFQQILAALFGYMLNRFELKPMLQKA	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	306 (290)
Sequences:	6028 (3561)
Seq/√Len:	209.1

ECOLI - YFEH
BACSU - YBAS YOCS ARSB

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
35_F	167_F	7.062	1.00
111_N	244_E	6.841	1.00
233_L	237_M	3.644	1.00
35_F	163_L	3.098	1.00
105_A	110_G	3.071	1.00
47_R	51_S	3.062	1.00
145_D	148_G	3.002	1.00
242_Q	246_V	2.968	1.00
236_K	245_I	2.744	1.00
241_R	244_E	2.726	1.00
89_L	219_T	2.635	1.00
224_A	286_L	2.457	1.00
63_L	97_T	2.432	1.00
260_G	276_V	2.402	1.00
235_G	245_I	2.315	1.00
8_S	192_M	2.289	1.00
174_E	178_F	2.143	1.00
235_G	248_L	2.139	1.00
4_L	8_S	2.129	1.00
91_L	264_A	2.123	1.00
61_F	65_I	2.120	1.00
44_Q	47_R	2.119	1.00
16_S	20_N	2.117	1.00
4_L	16_S	2.117	1.00
64_H	129_I	2.100	1.00
45_S	48_H	2.068	1.00
94_V	133_S	2.029	1.00
70_F	226_T	1.994	1.00
72_W	76_H	1.948	1.00
232_W	245_I	1.928	1.00
71_A	93_V	1.892	1.00
162_F	166_L	1.886	1.00
104_W	250_F	1.884	1.00
229_A	233_L	1.879	1.00
57_I	61_F	1.863	1.00
214_A	218_V	1.862	1.00
78_I	219_T	1.850	1.00
115_T	247_S	1.849	1.00
240_R	248_L	1.838	1.00
67_M	227_G	1.818	1.00
9_V	16_S	1.807	1.00
221_F	282_F	1.797	1.00
48_H	51_S	1.794	1.00
7_T	11_L	1.778	1.00
60_L	125_L	1.776	1.00
69_L	73_G	1.723	1.00
125_L	129_I	1.670	1.00
292_Y	296_R	1.659	1.00
8_S	16_S	1.657	1.00
42_N	168_N	1.654	1.00
290_F	294_L	1.652	1.00
166_L	170_M	1.645	1.00
282_F	286_L	1.624	1.00
75_G	86_I	1.621	1.00
16_S	19_L	1.621	1.00
105_A	247_S	1.589	1.00
73_G	77_L	1.558	1.00
147_W	151_K	1.530	1.00
74_S	219_T	1.529	1.00
58_L	238_M	1.510	1.00
105_A	115_T	1.501	1.00
62_V	231_A	1.499	1.00
17_Q	20_N	1.484	1.00
15_L	18_F	1.475	1.00
293_M	297_F	1.458	1.00
240_R	244_E	1.446	1.00
58_L	248_L	1.436	1.00
6_P	10_V	1.424	1.00
4_L	7_T	1.409	1.00
20_N	23_E	1.397	1.00
152_G	155_V	1.389	1.00
95_I	260_G	1.387	1.00
92_A	279_G	1.383	1.00
249_I	294_L	1.372	1.00
242_Q	297_F	1.369	1.00
53_P	57_I	1.363	1.00
70_F	227_G	1.356	1.00
84_L	271_Q	1.355	1.00
245_I	249_I	1.345	1.00
42_N	45_S	1.344	1.00
226_T	230_A	1.330	1.00
111_N	243_E	1.325	1.00
13_V	16_S	1.320	1.00
25_A	28_W	1.320	1.00
246_V	294_L	1.285	1.00
5_T	8_S	1.283	1.00
232_W	242_Q	1.275	1.00
6_P	9_V	1.274	1.00
168_N	177_A	1.272	1.00
167_F	171_S	1.271	1.00
257_I	280_M	1.270	1.00
108_Y	291_G	1.263	1.00
73_G	76_H	1.256	1.00
24_W	28_W	1.254	1.00
292_Y	295_N	1.243	1.00
97_T	119_I	1.243	1.00
28_W	31_A	1.242	1.00
62_V	252_G	1.225	1.00
154_I	159_I	1.223	1.00
133_S	137_L	1.216	1.00
64_H	122_D	1.190	1.00
165_M	169_Q	1.190	1.00
235_G	240_R	1.178	1.00
55_P	240_R	1.175	1.00
289_L	293_M	1.162	1.00
8_S	12_G	1.162	1.00
5_T	9_V	1.156	1.00
62_V	66_F	1.151	1.00
94_V	134_L	1.149	1.00
221_F	286_L	1.141	1.00
59_A	118_I	1.137	1.00
38_S	164_G	1.131	1.00
17_Q	21_G	1.114	1.00
172_S	176_T	1.097	1.00
44_Q	48_H	1.092	1.00
172_S	177_A	1.088	1.00
87_T	138_A	1.085	1.00
90_T	137_L	1.081	1.00
234_I	238_M	1.080	1.00
34_T	38_S	1.070	1.00
101_S	255_R	1.068	1.00
177_A	180_S	1.061	1.00
241_R	245_I	1.059	1.00
11_L	14_L	1.056	1.00
151_K	155_V	1.053	1.00
245_I	294_L	1.053	1.00
16_S	22_Y	1.052	1.00
4_L	192_M	1.048	1.00
56_M	121_V	1.045	1.00
4_L	9_V	1.043	1.00
18_F	21_G	1.037	1.00
9_V	12_G	1.031	1.00
186_F	190_C	1.027	1.00
60_L	121_V	1.026	1.00
10_V	13_V	1.026	1.00
55_P	114_L	1.026	1.00
126_S	130_V	1.021	1.00
225_L	229_A	1.006	1.00
85_T	216_I	1.004	1.00
129_I	132_L	1.004	1.00
16_S	21_G	0.998	1.00
69_L	137_L	0.995	1.00
117_S	120_L	0.992	1.00
63_L	255_R	0.986	1.00
5_T	16_S	0.977	1.00
54_L	240_R	0.975	1.00
250_F	254_M	0.975	1.00
48_H	117_S	0.973	1.00
90_T	94_V	0.964	1.00
52_H	111_N	0.963	1.00
6_P	16_S	0.961	1.00
113_G	116_L	0.961	1.00
232_W	249_I	0.960	1.00
82_D	215_G	0.958	1.00
98_G	101_S	0.956	1.00
4_L	20_N	0.951	0.99
62_V	234_I	0.943	0.99
148_G	151_K	0.941	0.99
203_A	207_K	0.939	0.99
7_T	10_V	0.938	0.99
102_L	251_T	0.937	0.99
10_V	14_L	0.931	0.99
25_A	197_I	0.931	0.99
129_I	133_S	0.930	0.99
233_L	236_K	0.929	0.99
9_V	13_V	0.928	0.99
144_M	149_M	0.924	0.99
82_D	86_I	0.922	0.99
16_S	23_E	0.913	0.99
260_G	275_P	0.903	0.99
91_L	130_V	0.902	0.99
176_T	180_S	0.902	0.99
89_L	220_V	0.898	0.99
8_S	11_L	0.896	0.99
76_H	80_K	0.894	0.99
279_G	284_Q	0.894	0.99
59_A	248_L	0.893	0.99
19_L	23_E	0.893	0.99
287_A	291_G	0.886	0.99
109_K	295_N	0.884	0.99
55_P	244_E	0.884	0.99
191_L	195_I	0.877	0.99
52_H	244_E	0.875	0.99
43_F	169_Q	0.873	0.99
81_G	85_T	0.871	0.99
58_L	240_R	0.868	0.99
45_S	113_G	0.867	0.99
13_V	207_K	0.865	0.99
155_V	159_I	0.864	0.99
17_Q	22_Y	0.863	0.99
19_L	22_Y	0.862	0.99
236_K	242_Q	0.858	0.99
225_L	289_L	0.851	0.99
9_V	17_Q	0.850	0.99
70_F	73_G	0.845	0.99
61_F	234_I	0.842	0.99
75_G	90_T	0.830	0.99
4_L	17_Q	0.830	0.99
29_I	190_C	0.830	0.99
236_K	239_K	0.828	0.99
97_T	250_F	0.825	0.99
12_G	16_S	0.821	0.99
174_E	177_A	0.818	0.98
202_I	205_Y	0.814	0.98
178_F	182_A	0.813	0.98
179_V	182_A	0.813	0.98
146_V	150_M	0.812	0.98
100_T	228_Y	0.810	0.98
196_A	200_S	0.809	0.98
144_M	148_G	0.809	0.98
123_T	259_A	0.808	0.98
280_M	284_Q	0.808	0.98
63_L	115_T	0.808	0.98
74_S	226_T	0.807	0.98
84_L	272_V	0.796	0.98
20_N	24_W	0.794	0.98
262_V	266_T	0.792	0.98
63_L	119_I	0.792	0.98
39_L	157_V	0.789	0.98
12_G	19_L	0.787	0.98
97_T	118_I	0.787	0.98
216_I	220_V	0.786	0.98
171_S	174_E	0.783	0.98
82_D	85_T	0.781	0.98
206_F	212_R	0.774	0.98
110_G	246_V	0.773	0.98
97_T	115_T	0.772	0.98
80_K	86_I	0.770	0.98
228_Y	231_A	0.769	0.98
205_Y	208_S	0.765	0.98
175_R	179_V	0.762	0.98
115_T	251_T	0.762	0.98
97_T	123_T	0.759	0.98
228_Y	290_F	0.758	0.97
114_L	244_E	0.756	0.97
81_G	215_G	0.755	0.97
8_S	17_Q	0.754	0.97
181_S	185_P	0.754	0.97
102_L	119_I	0.753	0.97
32_F	186_F	0.749	0.97
232_W	297_F	0.749	0.97
88_G	275_P	0.746	0.97
283_Q	287_A	0.743	0.97
143_H	147_W	0.742	0.97
123_T	256_N	0.741	0.97
150_M	154_I	0.741	0.97
122_D	255_R	0.739	0.97
31_A	163_L	0.737	0.97
235_G	239_K	0.735	0.97
122_D	129_I	0.734	0.97
166_L	169_Q	0.734	0.97
92_A	274_V	0.732	0.97
8_S	15_L	0.727	0.97
242_Q	245_I	0.718	0.97
4_L	13_V	0.714	0.96
118_I	248_L	0.713	0.96
46_L	49_A	0.713	0.96
98_G	251_T	0.713	0.96
99_I	104_W	0.707	0.96
66_F	230_A	0.707	0.96
78_I	222_F	0.705	0.96
128_L	132_L	0.703	0.96
60_L	117_S	0.703	0.96
8_S	13_V	0.702	0.96
13_V	205_Y	0.701	0.96
98_G	256_N	0.701	0.96
199_S	280_M	0.699	0.96
232_W	236_K	0.694	0.96
101_S	115_T	0.688	0.96
50_L	53_P	0.687	0.96
18_F	22_Y	0.687	0.96
243_E	247_S	0.685	0.95
67_M	93_V	0.684	0.95
13_V	203_A	0.682	0.95
29_I	194_V	0.681	0.95
36_A	187_S	0.680	0.95
252_G	255_R	0.680	0.95
132_L	136_L	0.674	0.95
11_L	17_Q	0.672	0.95
106_A	109_K	0.672	0.95
86_I	90_T	0.672	0.95
265_V	273_A	0.670	0.95
76_H	86_I	0.669	0.95
251_T	255_R	0.669	0.95
98_G	102_L	0.668	0.95
8_S	20_N	0.668	0.95
5_T	195_I	0.665	0.95
30_F	34_T	0.662	0.95
149_M	153_L	0.662	0.95
104_W	254_M	0.662	0.95
187_S	194_V	0.662	0.95
27_P	31_A	0.661	0.95
26_V	258_S	0.661	0.95
279_G	283_Q	0.659	0.94
228_Y	249_I	0.659	0.94
150_M	153_L	0.658	0.94
104_W	198_N	0.655	0.94
51_S	57_I	0.654	0.94
167_F	175_R	0.653	0.94
26_V	253_G	0.653	0.94
92_A	283_Q	0.652	0.94
261_A	265_V	0.652	0.94
132_L	135_S	0.650	0.94
146_V	149_M	0.649	0.94
99_I	257_I	0.642	0.94
277_V	281_L	0.642	0.94
116_L	119_I	0.641	0.94
55_P	248_L	0.640	0.94
152_G	156_M	0.639	0.93
177_A	237_M	0.639	0.93
104_W	283_Q	0.639	0.93
14_L	17_Q	0.637	0.93
63_L	251_T	0.637	0.93
105_A	261_A	0.636	0.93
257_I	283_Q	0.635	0.93
108_Y	250_F	0.635	0.93
288_A	292_Y	0.634	0.93
211_L	215_G	0.633	0.93
117_S	121_V	0.632	0.93
58_L	235_G	0.623	0.93
119_I	251_T	0.621	0.92
75_G	89_L	0.620	0.92
171_S	175_R	0.620	0.92
106_A	112_V	0.620	0.92
80_K	83_P	0.617	0.92
63_L	118_I	0.617	0.92
218_V	221_F	0.616	0.92
59_A	251_T	0.614	0.92
29_I	193_A	0.610	0.92
100_T	284_Q	0.607	0.92
249_I	253_G	0.606	0.92
98_G	254_M	0.605	0.92
95_I	126_S	0.604	0.91
11_L	15_L	0.603	0.91
97_T	100_T	0.602	0.91
276_V	283_Q	0.601	0.91
197_I	201_A	0.599	0.91
153_L	158_V	0.599	0.91
27_P	156_M	0.596	0.91
55_P	118_I	0.596	0.91
168_N	172_S	0.596	0.91
208_S	211_L	0.596	0.91
97_T	251_T	0.596	0.91
95_I	130_V	0.590	0.90
91_L	263_L	0.586	0.90
100_T	122_D	0.585	0.90
280_M	283_Q	0.582	0.90
212_R	216_I	0.582	0.90
74_S	222_F	0.582	0.90
103_I	191_L	0.581	0.90
87_T	134_L	0.581	0.90
290_F	293_M	0.580	0.90
73_G	226_T	0.576	0.89
168_N	180_S	0.575	0.89
126_S	133_S	0.575	0.89
166_L	217_A	0.575	0.89
183_L	186_F	0.574	0.89
59_A	63_L	0.574	0.89
55_P	59_A	0.574	0.89
48_H	52_H	0.572	0.89
7_T	14_L	0.571	0.89
86_I	89_L	0.571	0.89
13_V	21_G	0.570	0.89
166_L	171_S	0.570	0.89
163_L	167_F	0.570	0.89
147_W	150_M	0.570	0.89
60_L	118_I	0.568	0.89
15_L	20_N	0.567	0.89
254_M	287_A	0.566	0.89
71_A	75_G	0.565	0.89
111_N	241_R	0.564	0.88
75_G	78_I	0.563	0.88
225_L	286_L	0.562	0.88
128_L	184_S	0.557	0.88
191_L	202_I	0.556	0.88
182_A	185_P	0.555	0.88
220_V	224_A	0.554	0.88
235_G	249_I	0.554	0.88
18_F	204_P	0.551	0.87
58_L	234_I	0.551	0.87
172_S	175_R	0.550	0.87
180_S	184_S	0.550	0.87
228_Y	287_A	0.549	0.87
18_F	23_E	0.549	0.87
65_I	69_L	0.548	0.87
230_A	234_I	0.546	0.87
100_T	254_M	0.546	0.87
71_A	89_L	0.545	0.87
104_W	194_V	0.544	0.87
74_S	89_L	0.544	0.87
99_I	260_G	0.544	0.87
258_S	262_V	0.542	0.86
98_G	280_M	0.541	0.86
273_A	277_V	0.541	0.86
33_I	38_S	0.541	0.86
81_G	86_I	0.540	0.86
7_T	208_S	0.538	0.86
6_P	21_G	0.535	0.86
101_S	247_S	0.535	0.86
63_L	122_D	0.533	0.85
148_G	152_G	0.532	0.85
87_T	272_V	0.530	0.85
232_W	246_V	0.530	0.85
92_A	257_I	0.528	0.85
36_A	186_F	0.527	0.85
85_T	212_R	0.527	0.85
279_G	285_I	0.523	0.84
15_L	22_Y	0.521	0.84
34_T	37_G	0.521	0.84
100_T	283_Q	0.521	0.84
124_V	128_L	0.520	0.84
33_I	100_T	0.518	0.84
32_F	36_A	0.518	0.84
107_M	291_G	0.518	0.84
191_L	194_V	0.516	0.84
102_L	115_T	0.516	0.84
23_E	201_A	0.515	0.84
98_G	123_T	0.514	0.83
66_F	70_F	0.513	0.83
91_L	134_L	0.512	0.83
12_G	15_L	0.512	0.83
236_K	241_R	0.511	0.83
262_V	284_Q	0.511	0.83
68_P	255_R	0.510	0.83
191_L	258_S	0.510	0.83
255_R	283_Q	0.509	0.83
227_G	253_G	0.509	0.83
104_W	192_M	0.507	0.83
254_M	279_G	0.505	0.82
74_S	78_I	0.504	0.82
17_Q	221_F	0.502	0.82
94_V	123_T	0.502	0.82
4_L	10_V	0.501	0.82
17_Q	23_E	0.501	0.82
52_H	55_P	0.500	0.82

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4n7wA	2	0.9771	100	0.192	Contact Map
3zuxA	1	0.9575	100	0.208	Contact Map
4czbA	2	0.9379	97.6	0.864	Contact Map
4bwzA	2	0.9542	97.2	0.872	Contact Map
4cz8A	2	0.9346	94.5	0.899	Contact Map
1zcdA	1	0.9314	74.3	0.927	Contact Map
3w4tA	1	0.9739	39.3	0.942	Contact Map
4p6vD	1	0.5817	20.3	0.949	Contact Map
1pw4A	1	0.9314	19.4	0.95	Contact Map
2m20A	2	0.1242	18	0.951	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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