GREMLIN Database
MLEN - Malate-2H(+)/Na(+)-lactate antiporter
UniProt: P54571 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 468 (426)
Sequences: 2180 (1418)
Seq/√Len: 68.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
205_L435_W6.2871.00
126_T129_G4.9021.00
350_G449_Y4.0231.00
386_D392_R3.9811.00
246_L267_L3.9511.00
142_G152_V3.6381.00
118_S199_Y3.4051.00
131_V419_M3.3711.00
200_V204_I3.1501.00
348_S351_N3.0941.00
55_S58_D3.0261.00
190_A394_V2.9781.00
111_L207_T2.9371.00
111_L203_A2.9361.00
166_L192_L2.9321.00
139_I149_L2.9051.00
356_T385_Y2.7311.00
201_I439_V2.7041.00
350_G448_G2.6401.00
86_T287_A2.6181.00
279_D282_H2.5751.00
341_T344_K2.4691.00
247_V264_G2.4061.00
354_L449_Y2.3961.00
78_V309_I2.3641.00
110_L151_L2.3621.00
113_T138_M2.3021.00
102_E227_K2.2611.00
330_E379_K2.2511.00
434_M437_S2.2411.00
365_I410_V2.2351.00
95_T236_I2.2251.00
357_L402_G2.2241.00
65_H331_K2.2221.00
114_L199_Y2.2131.00
155_A206_F2.1941.00
359_V381_M2.1931.00
49_G59_L2.1931.00
364_N406_T2.1761.00
249_V253_M2.1231.00
269_A283_A2.1051.00
181_K392_R2.0641.00
390_L394_V2.0371.00
86_T265_A2.0191.00
357_L445_I2.0091.00
95_T99_Y2.0051.00
89_A286_T1.9901.00
264_G268_G1.9841.00
182_V392_R1.9691.00
435_W439_V1.9681.00
230_L236_I1.9651.00
65_H68_S1.9451.00
62_A331_K1.9401.00
204_I208_V1.9161.00
235_D238_I1.9151.00
427_T431_L1.9131.00
420_A427_T1.8961.00
202_T206_F1.8921.00
198_A439_V1.8761.00
207_T211_F1.8641.00
79_S258_M1.8451.00
92_V243_P1.8311.00
95_T241_L1.7911.00
201_I443_L1.7821.00
194_L401_V1.7791.00
442_G446_I1.7761.00
435_W438_F1.7681.00
183_D186_A1.7331.00
288_Y310_V1.7121.00
446_I450_T1.6680.99
285_A289_N1.6620.99
142_G149_L1.6530.99
250_L263_I1.6490.99
151_L209_V1.6480.99
360_A399_S1.6260.99
313_L317_V1.6240.99
147_I152_V1.6200.99
25_S30_L1.5810.99
177_S182_V1.5770.99
336_K340_S1.5730.99
153_A423_L1.5710.99
361_F406_T1.5690.99
18_V44_I1.5670.99
84_I132_G1.5640.99
99_Y238_I1.5460.99
43_F262_V1.5370.99
354_L445_I1.5330.99
273_V283_A1.5310.99
347_T351_N1.5270.99
70_G323_L1.5270.99
49_G332_L1.5210.99
353_T394_V1.4790.99
271_W275_F1.4770.99
434_M438_F1.4750.99
186_A393_R1.4750.99
418_Y422_I1.4690.99
177_S181_K1.4600.99
239_W271_W1.4600.99
172_S374_L1.4580.99
270_I274_V1.4460.98
158_S206_F1.4320.98
179_L378_P1.4260.98
209_V435_W1.4250.98
428_F431_L1.4060.98
357_L444_A1.4050.98
229_S301_N1.4020.98
382_E386_D1.3990.98
224_E228_S1.3950.98
240_M275_F1.3950.98
33_P37_A1.3880.98
398_N444_A1.3870.98
245_V249_V1.3830.98
201_I205_L1.3790.98
66_G327_G1.3790.98
157_L416_G1.3770.98
205_L209_V1.3670.98
407_S415_N1.3660.98
240_M271_W1.3620.98
447_Y453_F1.3620.98
89_A272_A1.3620.98
148_P213_Y1.3590.98
78_V313_L1.3570.98
152_V156_I1.3490.98
88_I306_R1.3420.97
165_K400_E1.3370.97
407_S413_S1.3350.97
61_N68_S1.3310.97
205_L439_V1.3310.97
182_V186_A1.3270.97
99_Y241_L1.3210.97
86_T269_A1.3180.97
11_E52_L1.3150.97
190_A393_R1.3100.97
154_G158_S1.3080.97
85_G89_A1.3010.97
202_T439_V1.2940.97
115_I203_A1.2880.97
431_L438_F1.2820.97
191_M400_E1.2800.97
146_G149_L1.2790.97
86_T268_G1.2770.97
378_P399_S1.2710.97
227_K231_Q1.2630.96
195_S440_A1.2600.96
198_A440_A1.2580.96
427_T430_Y1.2550.96
383_D387_R1.2380.96
24_L28_V1.2270.96
405_L440_A1.2260.96
110_L147_I1.2230.96
350_G445_I1.2150.95
401_V444_A1.2090.95
272_A278_M1.2070.95
135_G153_A1.2000.95
45_A328_L1.1890.95
139_I153_A1.1840.95
82_A287_A1.1840.95
409_M438_F1.1790.95
165_K404_T1.1740.95
13_I17_G1.1590.94
337_V341_T1.1590.94
400_E404_T1.1560.94
397_R401_V1.1550.94
203_A207_T1.1520.94
352_V384_S1.1460.94
75_L258_M1.1460.94
278_M286_T1.1440.94
181_K382_E1.1420.94
14_I44_I1.1410.94
289_N310_V1.1330.93
118_S162_F1.1310.93
239_W242_I1.1260.93
310_V313_L1.1230.93
161_Y419_M1.1220.93
56_Y259_P1.1190.93
384_S387_R1.1140.93
310_V314_D1.1120.93
384_S388_L1.1120.93
438_F442_G1.1100.93
99_Y236_I1.1080.93
138_M156_I1.0940.92
360_A403_G1.0920.92
369_A373_S1.0790.92
242_I271_W1.0790.92
162_F400_E1.0790.92
238_I245_V1.0780.92
173_T400_E1.0740.91
64_V68_S1.0650.91
353_T448_G1.0640.91
74_V319_I1.0610.91
58_D61_N1.0600.91
63_I324_G1.0580.91
92_V240_M1.0500.91
330_E336_K1.0470.90
100_G234_F1.0430.90
161_Y413_S1.0420.90
73_A371_Y1.0350.90
192_L196_I1.0320.90
424_G427_T1.0300.90
350_G354_L1.0300.90
383_D386_D1.0250.89
439_V443_L1.0240.89
237_H240_M1.0220.89
242_I267_L1.0180.89
113_T155_A1.0180.89
157_L367_G1.0120.89
18_V48_L1.0070.89
39_F82_A1.0050.89
104_I112_A1.0050.89
142_G146_G1.0040.88
187_H396_S1.0010.88
348_S388_L0.9970.88
443_L446_I0.9870.88
226_L300_L0.9840.87
116_I138_M0.9790.87
95_T240_M0.9770.87
11_E51_R0.9770.87
436_L440_A0.9760.87
370_M441_I0.9710.87
315_S415_N0.9710.87
104_I108_I0.9690.87
150_P429_S0.9670.87
198_A443_L0.9650.86
61_N65_H0.9650.86
450_T453_F0.9640.86
250_L255_K0.9630.86
57_K61_N0.9630.86
74_V316_L0.9620.86
349_A388_L0.9620.86
34_I37_A0.9590.86
139_I143_E0.9570.86
361_F365_I0.9480.85
121_S130_T0.9460.85
139_I425_V0.9440.85
278_M282_H0.9440.85
307_G311_G0.9400.85
67_I75_L0.9390.85
110_L148_P0.9380.85
117_C121_S0.9370.85
166_L196_I0.9360.85
117_C134_A0.9320.84
224_E227_K0.9310.84
180_S396_S0.9310.84
346_L352_V0.9300.84
194_L397_R0.9300.84
195_S400_E0.9270.84
62_A327_G0.9230.84
79_S265_A0.9210.84
426_S429_S0.9200.84
57_K115_I0.9200.84
409_M434_M0.9150.83
246_L249_V0.9140.83
175_L374_L0.9080.83
202_T205_L0.9070.83
34_I414_D0.9060.83
165_K195_S0.9020.82
123_A248_I0.8990.82
410_V413_S0.8940.82
225_F229_S0.8870.81
246_L263_I0.8860.81
157_L419_M0.8840.81
197_P200_V0.8800.81
197_P443_L0.8790.81
341_T345_K0.8760.81
128_W418_Y0.8670.80
47_L51_R0.8670.80
198_A202_T0.8620.79
118_S122_V0.8620.79
225_F228_S0.8610.79
153_A425_V0.8570.79
357_L448_G0.8550.79
118_S121_S0.8500.79
187_H400_E0.8500.79
34_I38_L0.8490.78
405_L436_L0.8450.78
425_V429_S0.8410.78
343_E387_R0.8400.78
90_G271_W0.8340.77
416_G430_Y0.8300.77
81_G312_M0.8300.77
148_P433_F0.8270.77
346_L351_N0.8260.77
143_E149_L0.8260.77
349_A390_L0.8240.76
354_L358_I0.8210.76
443_L447_Y0.8210.76
374_L404_T0.8180.76
235_D274_V0.8170.76
145_L152_V0.8160.76
314_D317_V0.8120.75
165_K187_H0.8090.75
86_T283_A0.8060.75
181_K386_D0.8030.74
67_I320_I0.8000.74
172_S400_E0.7970.74
338_I347_T0.7940.74
385_Y395_L0.7930.74
161_Y206_F0.7920.73
173_T176_A0.7880.73
74_V320_I0.7880.73
82_A265_A0.7880.73
85_G283_A0.7870.73
447_Y450_T0.7860.73
255_K263_I0.7850.73
139_I156_I0.7850.73
190_A390_L0.7840.73
126_T370_M0.7840.73
22_L37_A0.7820.73
420_A426_S0.7810.72
257_S303_L0.7810.72
46_M328_L0.7720.72
445_I449_Y0.7700.71
363_A377_T0.7670.71
42_W46_M0.7670.71
19_F436_L0.7650.71
373_S377_T0.7640.71
369_A407_S0.7610.70
346_L355_S0.7610.70
288_Y314_D0.7610.70
115_I231_Q0.7590.70
42_W321_F0.7590.70
23_V27_T0.7570.70
42_W325_F0.7560.70
54_Y58_D0.7530.70
385_Y398_N0.7520.70
357_L361_F0.7480.69
237_H275_F0.7480.69
45_A48_L0.7470.69
206_F435_W0.7470.69
21_A233_T0.7460.69
406_T437_S0.7450.69
46_M50_I0.7430.69
158_S434_M0.7410.68
15_V45_A0.7400.68
370_M407_S0.7390.68
17_G232_N0.7390.68
231_Q266_L0.7370.68
129_G243_P0.7370.68
171_D176_A0.7370.68
57_K340_S0.7340.68
369_A413_S0.7280.67
165_K171_D0.7280.67
416_G420_A0.7270.67
130_T160_A0.7250.67
89_A92_V0.7230.67
369_A414_D0.7220.66
108_I111_L0.7210.66
272_A283_A0.7210.66
224_E316_L0.7200.66
208_V233_T0.7180.66
166_L172_S0.7170.66
228_S231_Q0.7160.66
173_T188_V0.7160.66
15_V19_F0.7150.66
202_T442_G0.7150.66
127_S172_S0.7140.66
306_R311_G0.7140.66
76_I80_V0.7140.66
353_T454_I0.7140.66
309_I317_V0.7130.65
49_G355_S0.7110.65
83_L261_I0.7100.65
126_T371_Y0.7100.65
127_S160_A0.7090.65
193_Y452_K0.7090.65
151_L158_S0.7080.65
370_M415_N0.7080.65
142_G145_L0.7070.65
118_S284_I0.7060.65
127_S171_D0.7040.64
267_L271_W0.7030.64
277_G309_I0.7030.64
323_L364_N0.7030.64
145_L210_G0.7020.64
174_V184_V0.7000.64
432_P435_W0.6990.64
353_T398_N0.6990.64
111_L121_S0.6980.64
374_L399_S0.6970.64
17_G249_V0.6950.63
164_D400_E0.6940.63
187_H191_M0.6930.63
377_T403_G0.6920.63
340_S387_R0.6910.63
157_L434_M0.6900.63
66_G323_L0.6890.63
36_L376_L0.6870.63
172_S177_S0.6860.62
137_A320_I0.6850.62
77_L161_Y0.6820.62
182_V391_D0.6820.62
176_A187_H0.6800.62
263_I267_L0.6790.62
394_V398_N0.6790.62
90_G390_L0.6790.62
286_T309_I0.6770.61
119_I245_V0.6760.61
323_L378_P0.6760.61
408_G437_S0.6740.61
195_S401_V0.6720.61
251_L268_G0.6720.61
81_G308_G0.6690.61
132_G136_I0.6650.60
30_L280_I0.6630.60
49_G250_L0.6620.60
15_V334_V0.6610.60
73_A129_G0.6600.59
130_T371_Y0.6600.59
204_I349_A0.6590.59
180_S392_R0.6590.59
200_V279_D0.6580.59
77_L80_V0.6580.59
110_L142_G0.6560.59
420_A430_Y0.6550.59
113_T117_C0.6540.59
161_Y406_T0.6520.59
216_K270_I0.6520.59
408_G419_M0.6520.59
205_L208_V0.6520.59
273_V280_I0.6510.58
405_L441_I0.6510.58
174_V178_S0.6510.58
129_G371_Y0.6480.58
80_V131_V0.6460.58
55_S347_T0.6460.58
394_V448_G0.6440.58
161_Y437_S0.6440.58
364_N407_S0.6430.57
366_F408_G0.6420.57
114_L129_G0.6410.57
249_V252_A0.6400.57
235_D275_F0.6400.57
394_V447_Y0.6360.57
193_Y197_P0.6340.56
18_V49_G0.6330.56
241_L268_G0.6330.56
192_L205_L0.6310.56
93_V322_G0.6300.56
131_V261_I0.6290.56
46_M59_L0.6280.56
77_L316_L0.6260.55
364_N373_S0.6260.55
98_Y102_E0.6260.55
356_T398_N0.6230.55
340_S442_G0.6230.55
281_A435_W0.6220.55
117_C130_T0.6220.55
105_H112_A0.6200.55
36_L41_S0.6180.55
142_G147_I0.6180.55
115_I438_F0.6170.54
129_G370_M0.6150.54
173_T187_H0.6140.54
42_W437_S0.6140.54
256_P259_P0.6140.54
402_G444_A0.6140.54
190_A397_R0.6140.54
157_L413_S0.6110.54
352_V356_T0.6100.54
274_V277_G0.6090.53
439_V442_G0.6090.53
42_W63_I0.6080.53
375_I410_V0.6080.53
174_V377_T0.6070.53
209_V433_F0.6070.53
273_V282_H0.6060.53
118_S203_A0.6050.53
74_V93_V0.6030.53
360_A377_T0.6030.53
322_G326_G0.6020.53
240_M244_A0.6000.52
11_E14_I0.5990.52
182_V366_F0.5990.52
27_T261_I0.5980.52
132_G418_Y0.5980.52
279_D286_T0.5980.52
359_V396_S0.5960.52
382_E392_R0.5950.52
122_V171_D0.5950.52
104_I127_S0.5930.52
364_N370_M0.5920.51
50_I59_L0.5850.51
183_D189_R0.5850.51
11_E48_L0.5850.51
264_G375_I0.5850.51
38_L77_L0.5840.50
46_M49_G0.5840.50
357_L398_N0.5840.50
85_G240_M0.5820.50
398_N402_G0.5820.50
47_L50_I0.5810.50
77_L369_A0.5800.50
202_T435_W0.5780.50
442_G445_I0.5780.50
362_L366_F0.5780.50
138_M417_I0.5780.50
94_P234_F0.5760.49
19_F23_V0.5760.49
47_L386_D0.5750.49
282_H286_T0.5750.49
313_L316_L0.5740.49
36_L50_I0.5740.49
161_Y408_G0.5730.49
100_G137_A0.5730.49
195_S404_T0.5720.49
243_P251_L0.5720.49
48_L52_L0.5720.49
21_A49_G0.5710.49
172_S176_A0.5710.49
27_T34_I0.5700.49
80_V261_I0.5700.49
232_N362_L0.5700.49
39_F321_F0.5700.49
164_D170_S0.5680.49
199_Y203_A0.5680.49
108_I112_A0.5680.49
368_C407_S0.5670.48
281_A285_A0.5660.48
178_S441_I0.5650.48
450_T454_I0.5650.48
329_L332_L0.5650.48
209_V213_Y0.5650.48
111_L372_V0.5640.48
64_V71_L0.5640.48
208_V212_M0.5640.48
319_I372_V0.5630.48
167_S308_G0.5630.48
199_Y436_L0.5620.48
85_G286_T0.5620.48
128_W440_A0.5610.48
121_S168_P0.5610.48
55_S196_I0.5600.48
96_L171_D0.5600.48
20_L363_A0.5590.47
244_A261_I0.5570.47
139_I422_I0.5570.47
19_F45_A0.5570.47
345_K348_S0.5560.47
12_I337_V0.5550.47
216_K246_L0.5540.47
343_E346_L0.5530.47
250_L260_V0.5500.46
109_F149_L0.5500.46
110_L155_A0.5500.46
191_M338_I0.5490.46
15_V240_M0.5490.46
128_W414_D0.5480.46
34_I314_D0.5480.46
94_P304_L0.5460.46
389_H392_R0.5440.46
190_A453_F0.5440.46
200_V249_V0.5420.45
41_S45_A0.5420.45
362_L387_R0.5410.45
41_S439_V0.5410.45
128_W407_S0.5400.45
211_F242_I0.5400.45
16_L63_I0.5400.45
75_L93_V0.5390.45
118_S436_L0.5390.45
229_S232_N0.5380.45
301_N318_V0.5380.45
54_Y331_K0.5370.45
49_G334_V0.5370.45
172_S415_N0.5370.45
317_V380_I0.5360.45
121_S284_I0.5360.45
130_T144_G0.5360.45
425_V431_L0.5350.45
153_A419_M0.5340.44
107_S232_N0.5340.44
29_F375_I0.5300.44
349_A451_G0.5300.44
187_H404_T0.5290.44
16_L366_F0.5280.44
124_T210_G0.5280.44
65_H246_L0.5270.44
346_L381_M0.5260.44
162_F166_L0.5260.44
100_G168_P0.5250.43
303_L390_L0.5250.43
234_F276_Q0.5240.43
141_I234_F0.5240.43
40_V284_I0.5220.43
60_Q93_V0.5210.43
343_E384_S0.5210.43
124_T130_T0.5210.43
139_I148_P0.5200.43
228_S232_N0.5200.43
27_T35_Q0.5200.43
46_M132_G0.5190.43
29_F206_F0.5190.43
157_L341_T0.5190.43
166_L188_V0.5190.43
114_L243_P0.5180.43
236_I240_M0.5160.42
17_G344_K0.5160.42
92_V179_L0.5150.42
12_I15_V0.5150.42
370_M436_L0.5140.42
408_G415_N0.5140.42
246_L250_L0.5130.42
348_S449_Y0.5130.42
104_I119_I0.5130.42
252_A358_I0.5130.42
111_L409_M0.5120.42
30_L151_L0.5120.42
165_K173_T0.5120.42
235_D239_W0.5110.42
39_F323_L0.5110.42
59_L332_L0.5110.42
62_A318_V0.5110.42
147_I213_Y0.5100.42
33_P314_D0.5100.42
266_L270_I0.5090.42
398_N448_G0.5090.42
155_A198_A0.5090.42
43_F280_I0.5080.41
426_S431_L0.5070.41
129_G372_V0.5070.41
123_A169_L0.5060.41
115_I442_G0.5050.41
115_I302_G0.5050.41
103_F209_V0.5050.41
196_I216_K0.5040.41
248_I252_A0.5040.41
317_V320_I0.5040.41
150_P428_F0.5040.41
154_G376_L0.5030.41
244_A251_L0.5030.41
67_I322_G0.5030.41
184_V417_I0.5020.41
360_A402_G0.5020.41
46_M63_I0.5010.41
50_I55_S0.5010.41
71_L74_V0.5010.41
372_V390_L0.5010.41
206_F436_L0.5000.40
204_I207_T0.5000.40
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4f35D 2 0.859 99.9 0.815 Contact Map
4r1iA 2 0.9252 99.8 0.841 Contact Map
4r0cA 3 0.4573 99.6 0.871 Contact Map
4pd6A 3 0.4316 69.5 0.968 Contact Map
3qe7A 2 0.7457 55.5 0.971 Contact Map
4rp9A 2 0.438 35.3 0.974 Contact Map
4eneA 2 0.6709 26.7 0.976 Contact Map
3nd0A 2 0.656 23.9 0.977 Contact Map
4bwzA 2 0.6261 19.7 0.977 Contact Map
4czbA 2 0.6688 14.5 0.979 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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