GREMLIN Database
MISCB - Membrane protein insertase MisCB
UniProt: P54544 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 275 (214)
Sequences: 4116 (2642)
Seq/√Len: 180.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
191_F229_T4.1441.00
128_K132_E4.1161.00
104_D107_Q3.9021.00
125_E128_K3.5371.00
102_Q105_S3.5161.00
100_K104_D3.1851.00
106_I122_L2.8981.00
50_A54_G2.7361.00
104_D108_V2.7001.00
102_Q132_E2.6821.00
193_Q247_G2.6741.00
68_I245_T2.4611.00
172_W179_D2.3661.00
43_Y47_P2.3451.00
103_V126_M2.2521.00
59_F69_L2.2521.00
102_Q133_H2.2431.00
115_D118_K2.2401.00
189_M244_F2.2191.00
149_S153_I2.2041.00
99_I135_I2.1211.00
107_Q111_K2.0991.00
122_L126_M2.0971.00
76_I249_F2.0491.00
227_M231_F2.0411.00
156_Y160_R2.0371.00
99_I129_L1.9931.00
107_Q122_L1.9361.00
153_I157_Y1.9171.00
106_I129_L1.9031.00
117_E124_M1.7671.00
199_K222_F1.7651.00
174_S177_Q1.7451.00
79_L83_V1.7221.00
67_I238_A1.7211.00
124_M128_K1.7021.00
105_S109_K1.6721.00
56_A63_Y1.6271.00
109_K125_E1.6261.00
146_L150_P1.6041.00
252_V256_V1.5761.00
218_K222_F1.5351.00
123_Q127_M1.5181.00
197_A251_T1.5111.00
118_K125_E1.4681.00
55_V69_L1.4651.00
181_L185_S1.4601.00
195_Y222_F1.4481.00
108_V111_K1.4261.00
195_Y199_K1.4231.00
126_M130_Y1.4071.00
95_K98_V1.3981.00
53_K57_G1.3941.00
170_F173_F1.3921.00
83_V87_K1.3881.00
123_Q126_M1.3831.00
103_V129_L1.3791.00
157_Y161_S1.3761.00
145_M149_S1.3561.00
211_P214_Q1.3441.00
90_R97_A1.3401.00
199_K203_K1.3321.00
219_L223_I1.3321.00
65_L68_I1.3221.00
105_S108_V1.3081.00
157_Y160_R1.3061.00
256_V260_T1.3031.00
55_V73_I1.2951.00
78_V151_I1.2911.00
75_R250_L1.2851.00
192_V196_I1.2791.00
65_L69_L1.2401.00
219_L222_F1.2241.00
73_I77_V1.2231.00
85_Q89_Q1.2131.00
52_L66_S1.2071.00
248_L252_V1.2031.00
75_R253_Q1.1981.00
179_D185_S1.1751.00
54_G58_L1.1721.00
224_F227_M1.1631.00
188_I192_V1.1571.00
216_S219_L1.1481.00
84_N87_K1.1441.00
111_K116_P1.1341.00
76_I79_L1.1301.00
51_L70_V1.1211.00
215_Q219_L1.1131.00
216_S220_M1.1091.00
232_S240_P1.1071.00
65_L170_F1.0981.00
255_I259_M1.0961.00
226_V230_I1.0921.00
49_S162_T1.0851.00
230_I234_N1.0841.00
229_T233_L1.0721.00
182_M185_S1.0621.00
163_P166_A1.0621.00
180_I183_S1.0541.00
182_M233_L1.0431.00
111_K122_L1.0421.00
180_I185_S1.0271.00
223_I227_M1.0210.99
252_V255_I1.0150.99
169_S172_W1.0090.99
55_V70_V0.9810.99
180_I184_L0.9780.99
172_W237_A0.9680.99
56_A66_S0.9680.99
110_L118_K0.9650.99
64_G170_F0.9560.99
99_I133_H0.9530.99
118_K124_M0.9490.99
68_I241_L0.9480.99
215_Q218_K0.9470.99
71_T246_S0.9450.99
189_M193_Q0.9350.99
193_Q197_A0.9320.99
87_K95_K0.9210.99
99_I130_Y0.9180.99
112_K118_K0.9150.99
72_I249_F0.9130.99
84_N88_K0.9110.99
178_S182_M0.9040.99
235_V243_W0.9010.99
128_K131_Q0.8980.99
247_G250_L0.8920.99
243_W247_G0.8800.98
64_G241_L0.8650.98
196_I199_K0.8620.98
127_M131_Q0.8550.98
130_Y137_P0.8530.98
58_L61_G0.8520.98
246_S250_L0.8480.98
193_Q244_F0.8470.98
118_K122_L0.8310.98
210_N214_Q0.8260.98
78_V148_Q0.8240.98
35_S39_F0.8240.98
46_E50_A0.8230.98
163_P167_S0.8220.98
235_V239_L0.8210.98
98_V133_H0.8170.97
179_D182_M0.8110.97
75_R78_V0.8100.97
193_Q248_L0.8090.97
59_F65_L0.8070.97
80_P84_N0.8070.97
72_I245_T0.8010.97
83_V257_L0.7990.97
102_Q129_L0.7990.97
221_V225_P0.7910.97
212_A215_Q0.7860.97
55_V59_F0.7850.97
129_L133_H0.7820.97
232_S243_W0.7820.97
198_Q201_S0.7800.97
200_L255_I0.7790.97
95_K133_H0.7770.97
125_E132_E0.7770.97
54_G57_G0.7760.97
149_S152_M0.7710.96
57_G61_G0.7700.96
260_T263_K0.7660.96
75_R82_F0.7620.96
75_R246_S0.7580.96
197_A250_L0.7540.96
49_S53_K0.7540.96
169_S174_S0.7530.96
226_V229_T0.7500.96
184_L241_L0.7450.96
184_L237_A0.7440.96
156_Y235_V0.7430.96
70_V155_L0.7410.96
50_A53_K0.7400.96
42_D46_E0.7380.95
136_N142_C0.7340.95
55_V66_S0.7330.95
75_R148_Q0.7330.95
51_L54_G0.7320.95
79_L256_V0.7290.95
214_Q218_K0.7290.95
118_K128_K0.7260.95
190_Y193_Q0.7190.95
67_I159_I0.7160.95
214_Q217_A0.7110.94
36_N40_F0.7100.94
244_F248_L0.7060.94
106_I111_K0.6990.94
98_V265_K0.6990.94
83_V86_F0.6980.94
45_I161_S0.6960.94
223_I226_V0.6950.94
136_N139_A0.6930.94
190_Y229_T0.6930.94
231_F235_V0.6880.93
145_M148_Q0.6850.93
191_F195_Y0.6840.93
47_P51_L0.6810.93
42_D231_F0.6810.93
190_Y197_A0.6800.93
39_F43_Y0.6800.93
109_K122_L0.6750.93
90_R258_Q0.6750.93
86_F89_Q0.6710.93
227_M230_I0.6670.92
112_K122_L0.6670.92
172_W184_L0.6630.92
107_Q130_Y0.6610.92
55_V58_L0.6600.92
135_I139_A0.6570.92
56_A59_F0.6570.92
169_S173_F0.6570.92
36_N39_F0.6550.92
152_M250_L0.6550.92
172_W178_S0.6480.91
229_T232_S0.6470.91
42_D45_I0.6460.91
116_P123_Q0.6450.91
110_L122_L0.6450.91
86_F257_L0.6430.91
241_L245_T0.6420.91
152_M246_S0.6420.91
82_F257_L0.6370.91
64_G68_I0.6370.91
194_A247_G0.6360.90
179_D237_A0.6360.90
49_S158_A0.6350.90
106_I126_M0.6340.90
124_M127_M0.6310.90
88_K141_G0.6280.90
213_A218_K0.6250.90
172_W182_M0.6200.89
198_Q202_A0.6200.89
193_Q251_T0.6200.89
111_K118_K0.6190.89
165_I176_G0.6140.89
38_G41_H0.6140.89
96_M100_K0.6090.88
184_L244_F0.6070.88
74_V155_L0.6050.88
260_T264_S0.5960.87
85_Q139_A0.5940.87
173_F237_A0.5920.87
181_L184_L0.5900.87
70_V74_V0.5900.87
74_V242_Y0.5890.87
89_Q96_M0.5860.87
190_Y244_F0.5850.86
197_A200_L0.5850.86
69_L73_I0.5850.86
95_K134_N0.5840.86
162_T165_I0.5790.86
47_P156_Y0.5780.86
178_S181_L0.5720.85
144_P225_P0.5710.85
51_L73_I0.5710.85
63_Y165_I0.5710.85
65_L171_L0.5700.85
85_Q151_I0.5700.85
68_I170_F0.5680.85
44_L154_G0.5680.85
211_P215_Q0.5660.85
151_I155_L0.5650.84
75_R264_S0.5650.84
191_F194_A0.5640.84
172_W185_S0.5590.84
75_R249_F0.5560.84
56_A61_G0.5560.84
82_F86_F0.5470.83
82_F85_Q0.5460.82
99_I102_Q0.5440.82
45_I153_I0.5430.82
166_A174_S0.5430.82
56_A62_E0.5420.82
201_S208_P0.5370.81
83_V256_V0.5370.81
180_I188_I0.5360.81
175_L241_L0.5350.81
116_P122_L0.5350.81
72_I76_I0.5330.81
251_T255_I0.5330.81
179_D184_L0.5310.81
156_Y243_W0.5310.81
127_M137_P0.5300.81
202_A221_V0.5290.80
195_Y198_Q0.5250.80
99_I103_V0.5240.80
41_H47_P0.5240.80
48_F51_L0.5220.80
166_A176_G0.5210.79
122_L125_E0.5190.79
78_V85_Q0.5190.79
216_S221_V0.5190.79
40_F43_Y0.5160.79
81_L236_P0.5150.79
45_I165_I0.5150.79
167_S233_L0.5140.79
210_N215_Q0.5130.78
105_S110_L0.5130.78
232_S235_V0.5130.78
191_F228_M0.5130.78
190_Y228_M0.5130.78
85_Q145_M0.5120.78
150_P154_G0.5120.78
178_S234_N0.5120.78
95_K135_I0.5090.78
168_H172_W0.5090.78
197_A201_S0.5090.78
90_R261_H0.5080.78
154_G159_I0.5080.78
147_I150_P0.5070.78
152_M235_V0.5070.78
193_Q196_I0.5060.77
85_Q255_I0.5030.77
47_P50_A0.5030.77
190_Y239_L0.5030.77
84_N139_A0.5020.77
107_Q126_M0.5010.77
174_S179_D0.5000.77
36_N41_H0.5000.77
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3wvfA 1 0.7855 100 0.197 Contact Map
3wo6A 1 0.7636 100 0.21 Contact Map
3blcA 4 0.1636 22.8 0.955 Contact Map
3pjsK 4 0.44 7.8 0.963 Contact Map
3effK 4 0.44 5.9 0.965 Contact Map
3rvyA 3 0.3091 5 0.966 Contact Map
3vouA 4 0.4073 4.5 0.967 Contact Map
4il3A 1 0.7236 3.3 0.969 Contact Map
4c7rA 3 0.4327 2.7 0.97 Contact Map
4n7bA 1 0 2.6 0.971 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0081 seconds.