YQJE - BACSU - GREMLIN Contacts

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YQJE - Uncharacterized protein YqjE

UniProt:	P54542	Sequences: MVNEKRLLEEFLELVQIDSETKHEAEICKVLKRKFSDLGVDVKEDDTMDITGHGAGNLICTLKGTKQTDTIYFTSHMDTVVPGNGVKPVVENGYVKTDGTTILGADDKAGLAAMFEAIKVLKEENIEHGTIEFIITVGEESGLIGAKALDRSMITASYGYALDSDGKVGNIIVAAPTQAKVRAAIFGKTAHAGVEPEKGISAITIASKAISKMPLGRIDEETTANIGRFEGGTQTNIVCDEVHILAEARSLVPEKMEAQVQKMKAAFEEAAADMGGRAEVEIEVMYPGFKYQDGDQVVEIAKKAAAKIGRPSELQTSGGGSDANVIAGHGIPTVNLAVGYEQIHTKNEKMPIEELVKTAEMVVAIIEEAAK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	371 (340)
Sequences:	29682 (21140)
Seq/√Len:	1146.5

ECOLI - PEPT DAPE YGEY ARGE ABGA PEPD ALLC ABGB
BACSU - YQJE PEPT YODQ FAPD DAPEL YXEP YTNL YHAA AMHX PEPVL ALLC YQJN ROCB
HALSA - Q9HS25
SULSO - Q97Y12 Q97ZB7 LYSK CBPX1 CBPX2 Q97V97 Q97VB6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
61_T	132_E	3.435	1.00
184_A	241_E	3.171	1.00
32_K	36_S	2.979	1.00
205_I	276_G	2.795	1.00
184_A	243_H	2.793	1.00
13_E	30_V	2.755	1.00
94_Y	349_K	2.680	1.00
32_K	44_E	2.674	1.00
190_A	199_G	2.595	1.00
63_K	130_T	2.576	1.00
70_T	130_T	2.551	1.00
182_R	243_H	2.550	1.00
185_I	209_A	2.493	1.00
264_K	268_E	2.480	1.00
186_F	279_E	2.451	1.00
186_F	277_R	2.410	1.00
184_A	279_E	2.376	1.00
260_V	264_K	2.376	1.00
307_K	364_A	2.361	1.00
34_K	37_D	2.340	1.00
186_F	241_E	2.334	1.00
35_F	60_C	2.331	1.00
120_V	363_V	2.234	1.00
206_A	242_V	2.231	1.00
182_R	245_L	2.215	1.00
206_A	244_I	2.213	1.00
182_R	281_E	2.181	1.00
30_V	34_K	2.117	1.00
206_A	229_F	2.111	1.00
261_Q	265_A	2.105	1.00
230_E	243_H	2.090	1.00
61_T	130_T	2.062	1.00
70_T	132_E	2.059	1.00
120_V	124_E	2.023	1.00
184_A	281_E	2.011	1.00
207_S	210_I	1.898	1.00
308_I	364_A	1.888	1.00
302_K	306_A	1.885	1.00
87_K	347_N	1.876	1.00
86_V	90_V	1.874	1.00
38_L	119_K	1.856	1.00
147_K	151_R	1.825	1.00
228_R	245_L	1.819	1.00
301_A	365_I	1.814	1.00
17_I	30_V	1.807	1.00
208_K	211_S	1.762	1.00
88_P	91_E	1.759	1.00
29_K	33_R	1.758	1.00
33_R	37_D	1.747	1.00
209_A	267_F	1.719	1.00
202_A	242_V	1.705	1.00
40_V	62_L	1.686	1.00
26_E	29_K	1.680	1.00
268_E	272_A	1.657	1.00
364_A	367_E	1.653	1.00
188_K	200_I	1.652	1.00
34_K	38_L	1.639	1.00
306_A	311_P	1.631	1.00
12_L	16_Q	1.620	1.00
257_E	261_Q	1.618	1.00
7_L	359_A	1.607	1.00
93_G	353_E	1.600	1.00
32_K	58_L	1.594	1.00
10_E	34_K	1.587	1.00
208_K	274_M	1.587	1.00
13_E	16_Q	1.580	1.00
262_K	265_A	1.578	1.00
207_S	211_S	1.569	1.00
119_K	123_E	1.550	1.00
13_E	26_E	1.548	1.00
28_C	77_M	1.543	1.00
189_T	240_D	1.540	1.00
200_I	205_I	1.540	1.00
231_G	242_V	1.540	1.00
30_V	33_R	1.532	1.00
9_E	13_E	1.529	1.00
31_L	77_M	1.525	1.00
327_A	333_T	1.521	1.00
148_A	151_R	1.510	1.00
113_A	358_T	1.509	1.00
72_Y	134_I	1.507	1.00
204_T	214_P	1.505	1.00
299_E	302_K	1.490	1.00
41_D	63_K	1.486	1.00
29_K	44_E	1.483	1.00
212_K	269_E	1.465	1.00
195_E	198_K	1.462	1.00
33_R	36_S	1.458	1.00
36_S	44_E	1.450	1.00
255_K	258_A	1.447	1.00
64_G	130_T	1.440	1.00
181_V	246_A	1.425	1.00
13_E	17_I	1.416	1.00
179_A	256_M	1.384	1.00
258_A	262_K	1.380	1.00
62_L	122_K	1.374	1.00
212_K	266_A	1.374	1.00
182_R	283_E	1.372	1.00
260_V	282_I	1.368	1.00
185_I	206_A	1.365	1.00
32_K	42_V	1.352	1.00
208_K	273_D	1.351	1.00
148_A	152_S	1.348	1.00
36_S	42_V	1.346	1.00
261_Q	264_K	1.343	1.00
109_A	355_L	1.342	1.00
109_A	350_M	1.330	1.00
271_A	278_A	1.323	1.00
183_A	244_I	1.321	1.00
302_K	314_L	1.306	1.00
305_A	361_M	1.300	1.00
357_K	360_E	1.297	1.00
267_F	278_A	1.281	1.00
15_V	97_T	1.280	1.00
19_S	27_I	1.277	1.00
65_T	128_H	1.274	1.00
161_A	164_S	1.269	1.00
72_Y	156_A	1.264	1.00
73_F	114_M	1.260	1.00
304_A	364_A	1.259	1.00
25_A	29_K	1.256	1.00
34_K	119_K	1.255	1.00
264_K	280_V	1.248	1.00
9_E	12_L	1.246	1.00
169_G	174_A	1.228	1.00
157_S	161_A	1.226	1.00
265_A	269_E	1.222	1.00
25_A	56_G	1.216	1.00
10_E	30_V	1.215	1.00
200_I	276_G	1.215	1.00
356_V	360_E	1.212	1.00
10_E	116_E	1.207	1.00
7_L	116_E	1.206	1.00
122_K	125_N	1.200	1.00
220_E	252_V	1.192	1.00
29_K	32_K	1.189	1.00
185_I	205_I	1.179	1.00
265_A	268_E	1.171	1.00
8_L	12_L	1.169	1.00
68_T	129_G	1.169	1.00
300_I	304_A	1.163	1.00
308_I	360_E	1.160	1.00
304_A	308_I	1.157	1.00
14_L	27_I	1.149	1.00
222_T	259_Q	1.147	1.00
7_L	112_A	1.143	1.00
43_K	61_T	1.118	1.00
353_E	357_K	1.115	1.00
258_A	261_Q	1.109	1.00
73_F	366_I	1.109	1.00
21_T	141_S	1.105	1.00
203_I	229_F	1.101	1.00
295_D	299_E	1.100	1.00
203_I	231_G	1.099	1.00
299_E	303_K	1.094	1.00
15_V	100_T	1.090	1.00
124_E	363_V	1.089	1.00
31_L	111_L	1.080	1.00
301_A	334_V	1.079	1.00
124_E	367_E	1.075	1.00
12_L	352_I	1.071	1.00
41_D	61_T	1.070	1.00
260_V	284_V	1.065	1.00
157_S	163_D	1.059	1.00
210_I	244_I	1.054	1.00
57_N	137_V	1.053	1.00
351_P	354_E	1.046	1.00
252_V	255_K	1.043	1.00
28_C	58_L	1.030	1.00
167_K	170_N	1.029	1.00
69_D	129_G	1.029	1.00
85_G	347_N	1.026	1.00
40_V	60_C	1.026	1.00
94_Y	351_P	1.022	1.00
26_E	30_V	1.008	1.00
93_G	351_P	1.006	1.00
303_K	306_A	1.004	1.00
114_M	133_F	1.003	1.00
167_K	172_I	0.999	1.00
204_T	210_I	0.998	1.00
215_L	222_T	0.996	1.00
118_I	133_F	0.992	1.00
13_E	34_K	0.992	1.00
117_A	363_V	0.989	1.00
350_M	355_L	0.985	1.00
17_I	26_E	0.983	1.00
116_E	119_K	0.979	1.00
262_K	266_A	0.976	1.00
77_M	135_I	0.968	1.00
35_F	115_F	0.967	1.00
10_E	119_K	0.956	1.00
75_S	111_L	0.955	1.00
266_A	269_E	0.955	1.00
303_K	307_K	0.950	1.00
266_A	270_A	0.949	1.00
215_L	262_K	0.945	1.00
226_I	246_A	0.941	1.00
28_C	32_K	0.939	1.00
360_E	364_A	0.934	1.00
269_E	273_D	0.934	1.00
91_E	94_Y	0.930	1.00
7_L	355_L	0.930	1.00
22_K	138_G	0.927	1.00
14_L	30_V	0.925	1.00
77_M	111_L	0.925	1.00
164_S	343_I	0.924	1.00
306_A	310_R	0.919	1.00
298_V	334_V	0.913	1.00
83_G	92_N	0.911	1.00
42_V	60_C	0.909	1.00
38_L	122_K	0.909	1.00
206_A	210_I	0.907	1.00
183_A	267_F	0.903	1.00
120_V	360_E	0.903	1.00
169_G	173_V	0.900	1.00
181_V	260_V	0.897	1.00
167_K	174_A	0.895	1.00
65_T	127_E	0.894	1.00
62_L	118_I	0.893	1.00
203_I	242_V	0.890	1.00
208_K	212_K	0.888	1.00
363_V	367_E	0.886	1.00
268_E	271_A	0.885	1.00
230_E	233_T	0.883	1.00
359_A	363_V	0.881	1.00
183_A	209_A	0.880	1.00
147_K	325_V	0.875	1.00
300_I	365_I	0.875	1.00
212_K	270_A	0.874	1.00
253_P	257_E	0.873	1.00
117_A	362_V	0.870	1.00
118_I	122_K	0.870	1.00
213_M	263_M	0.868	1.00
69_D	157_S	0.868	1.00
182_R	235_T	0.862	1.00
217_R	220_E	0.861	1.00
304_A	307_K	0.858	1.00
204_T	207_S	0.858	1.00
162_L	362_V	0.856	1.00
234_Q	237_I	0.854	1.00
40_V	118_I	0.854	1.00
186_F	240_D	0.853	1.00
159_G	163_D	0.852	1.00
27_I	77_M	0.849	1.00
177_T	288_G	0.848	1.00
337_A	340_Y	0.843	1.00
210_I	226_I	0.842	1.00
354_E	357_K	0.840	1.00
87_K	90_V	0.838	1.00
174_A	316_T	0.836	1.00
323_A	333_T	0.835	1.00
180_K	247_E	0.835	1.00
13_E	33_R	0.830	1.00
34_K	115_F	0.830	1.00
300_I	303_K	0.824	1.00
296_Q	300_I	0.822	1.00
296_Q	299_E	0.822	1.00
79_T	98_D	0.816	1.00
235_T	245_L	0.814	1.00
121_L	126_I	0.813	1.00
229_F	242_V	0.809	1.00
59_I	132_E	0.806	1.00
197_E	217_R	0.806	1.00
159_G	164_S	0.805	1.00
269_E	272_A	0.803	1.00
175_A	321_S	0.803	1.00
270_A	273_D	0.801	1.00
73_F	117_A	0.799	1.00
208_K	214_P	0.787	1.00
156_A	161_A	0.785	1.00
216_G	222_T	0.785	1.00
177_T	250_S	0.785	1.00
355_L	359_A	0.778	1.00
257_E	260_V	0.773	1.00
210_I	229_F	0.771	1.00
167_K	171_I	0.769	1.00
43_K	153_M	0.765	1.00
28_C	44_E	0.763	1.00
298_V	302_K	0.761	1.00
213_M	224_A	0.761	1.00
146_A	150_D	0.761	1.00
294_G	297_V	0.757	1.00
70_T	157_S	0.756	1.00
22_K	141_S	0.754	1.00
254_E	258_A	0.753	1.00
159_G	331_I	0.749	1.00
31_L	115_F	0.748	1.00
321_S	335_N	0.745	1.00
305_A	308_I	0.744	1.00
11_F	95_V	0.743	1.00
200_I	275_G	0.743	1.00
265_A	272_A	0.739	1.00
226_I	244_I	0.739	1.00
190_A	238_V	0.738	1.00
21_T	24_E	0.733	1.00
68_T	130_T	0.730	1.00
181_V	282_I	0.729	1.00
120_V	359_A	0.727	1.00
113_A	362_V	0.726	1.00
353_E	356_V	0.726	1.00
43_K	59_I	0.724	1.00
183_A	280_V	0.724	1.00
185_I	244_I	0.724	1.00
20_E	81_V	0.722	1.00
28_C	56_G	0.721	1.00
325_V	328_G	0.718	1.00
117_A	359_A	0.716	1.00
71_I	366_I	0.714	1.00
287_P	328_G	0.713	1.00
62_L	133_F	0.713	1.00
64_G	67_Q	0.711	1.00
40_V	122_K	0.710	1.00
124_E	127_E	0.710	1.00
179_A	260_V	0.707	1.00
248_A	259_Q	0.706	1.00
63_K	67_Q	0.705	1.00
111_L	135_I	0.703	1.00
332_P	335_N	0.699	1.00
201_S	239_C	0.697	1.00
10_E	13_E	0.697	1.00
156_A	163_D	0.695	1.00
120_V	123_E	0.695	1.00
185_I	278_A	0.695	1.00
336_L	340_Y	0.693	1.00
205_I	274_M	0.691	1.00
301_A	361_M	0.690	1.00
42_V	58_L	0.690	1.00
91_E	96_K	0.689	1.00
31_L	135_I	0.689	1.00
257_E	284_V	0.687	1.00
342_Q	345_T	0.685	1.00
113_A	117_A	0.684	1.00
113_A	359_A	0.684	1.00
87_K	91_E	0.682	1.00
211_S	214_P	0.682	1.00
58_L	135_I	0.682	1.00
74_T	136_T	0.680	1.00
12_L	15_V	0.678	1.00
188_K	345_T	0.677	1.00
10_E	14_L	0.676	1.00
250_S	256_M	0.673	1.00
42_V	45_D	0.672	1.00
20_E	23_H	0.672	1.00
70_T	129_G	0.670	1.00
339_G	350_M	0.667	1.00
72_Y	157_S	0.666	1.00
208_K	270_A	0.664	1.00
158_Y	297_V	0.662	1.00
38_L	115_F	0.662	1.00
39_G	42_V	0.660	1.00
360_E	363_V	0.659	1.00
325_V	329_H	0.657	1.00
264_K	282_I	0.656	1.00
15_V	19_S	0.653	1.00
31_L	58_L	0.653	1.00
214_P	274_M	0.652	1.00
270_A	274_M	0.652	1.00
224_A	248_A	0.651	1.00
119_K	122_K	0.651	1.00
181_V	263_M	0.650	1.00
361_M	365_I	0.648	1.00
12_L	95_V	0.647	1.00
169_G	172_I	0.645	1.00
304_A	361_M	0.643	1.00
116_E	359_A	0.643	1.00
282_I	285_M	0.642	1.00
144_I	148_A	0.641	1.00
37_D	119_K	0.641	1.00
134_I	149_L	0.641	1.00
209_A	244_I	0.635	1.00
10_E	115_F	0.633	1.00
26_E	33_R	0.631	1.00
24_E	138_G	0.631	1.00
158_Y	161_A	0.630	1.00
256_M	287_P	0.629	1.00
202_A	239_C	0.625	1.00
40_V	133_F	0.625	1.00
10_E	112_A	0.622	1.00
305_A	312_S	0.622	1.00
64_G	68_T	0.621	1.00
236_N	249_R	0.621	1.00
8_L	356_V	0.620	1.00
159_G	162_L	0.620	1.00
8_L	95_V	0.619	1.00
308_I	361_M	0.617	1.00
140_E	344_H	0.615	1.00
304_A	365_I	0.613	1.00
204_T	211_S	0.612	1.00
176_P	223_T	0.612	1.00
46_D	56_G	0.609	1.00
336_L	361_M	0.609	1.00
96_K	349_K	0.608	1.00
150_D	329_H	0.607	1.00
35_F	40_V	0.606	1.00
11_F	352_I	0.601	1.00
267_F	280_V	0.600	1.00
21_T	142_G	0.596	1.00
85_G	346_K	0.595	1.00
224_A	227_G	0.594	1.00
342_Q	347_N	0.593	1.00
75_S	114_M	0.593	1.00
14_L	111_L	0.591	1.00
230_E	245_L	0.590	1.00
253_P	328_G	0.589	1.00
294_G	299_E	0.587	1.00
114_M	162_L	0.586	1.00
180_K	235_T	0.586	1.00
138_G	141_S	0.585	1.00
297_V	334_V	0.584	1.00
223_T	227_G	0.583	1.00
352_I	356_V	0.583	1.00
185_I	276_G	0.583	1.00
167_K	173_V	0.582	1.00
14_L	112_A	0.580	1.00
271_A	276_G	0.580	1.00
228_R	243_H	0.580	1.00
95_V	352_I	0.579	1.00
8_L	352_I	0.579	1.00
253_P	287_P	0.579	1.00
297_V	300_I	0.577	1.00
86_V	89_V	0.575	1.00
261_Q	268_E	0.575	1.00
209_A	270_A	0.575	1.00
160_Y	362_V	0.573	1.00
22_K	25_A	0.573	1.00
57_N	149_L	0.571	1.00
256_M	260_V	0.569	1.00
179_A	282_I	0.568	1.00
209_A	266_A	0.568	1.00
250_S	259_Q	0.567	1.00
143_L	286_Y	0.561	1.00
204_T	208_K	0.560	1.00
74_T	134_I	0.560	1.00
11_F	112_A	0.559	1.00
55_A	148_A	0.559	1.00
122_K	126_I	0.558	1.00
155_T	161_A	0.558	1.00
185_I	202_A	0.556	1.00
59_I	134_I	0.556	1.00
44_E	56_G	0.556	1.00
36_S	41_D	0.555	1.00
309_G	312_S	0.554	1.00
65_T	130_T	0.553	1.00
157_S	331_I	0.552	1.00
192_A	227_G	0.551	1.00
173_V	314_L	0.550	1.00
246_A	263_M	0.550	1.00
86_V	349_K	0.550	1.00
271_A	277_R	0.549	1.00
338_V	358_T	0.549	1.00
295_D	332_P	0.547	1.00
302_K	313_E	0.547	1.00
156_A	164_S	0.546	1.00
136_T	322_D	0.544	1.00
146_A	326_I	0.543	1.00
10_E	38_L	0.542	1.00
225_N	247_E	0.542	1.00
181_V	280_V	0.542	1.00
172_I	315_Q	0.540	1.00
64_G	125_N	0.539	1.00
209_A	213_M	0.539	1.00
43_K	46_D	0.535	1.00
126_I	367_E	0.533	1.00
323_A	335_N	0.532	1.00
65_T	68_T	0.531	1.00
157_S	164_S	0.531	1.00
200_I	277_R	0.530	1.00
45_D	152_S	0.529	1.00
17_I	27_I	0.529	1.00
20_E	94_Y	0.528	1.00
337_A	341_E	0.527	1.00
210_I	246_A	0.526	1.00
95_V	100_T	0.525	1.00
254_E	257_E	0.524	1.00
175_A	337_A	0.524	1.00
163_D	175_A	0.524	1.00
212_K	273_D	0.523	1.00
230_E	241_E	0.523	1.00
7_L	11_F	0.523	1.00
161_A	323_A	0.522	1.00
205_I	270_A	0.521	1.00
192_A	225_N	0.521	1.00
158_Y	332_P	0.521	1.00
138_G	144_I	0.519	1.00
286_Y	325_V	0.518	1.00
302_K	311_P	0.517	1.00
342_Q	349_K	0.517	1.00
75_S	110_G	0.516	1.00
248_A	254_E	0.515	1.00
97_T	100_T	0.514	1.00
263_M	280_V	0.514	1.00
189_T	202_A	0.513	1.00
336_L	339_G	0.513	1.00
71_I	158_Y	0.512	1.00
257_E	285_M	0.512	1.00
160_Y	164_S	0.512	1.00
176_P	318_G	0.511	1.00
272_A	277_R	0.510	1.00
190_A	195_E	0.510	1.00
207_S	214_P	0.510	1.00
77_M	137_V	0.509	1.00
69_D	128_H	0.509	1.00
141_S	144_I	0.509	1.00
160_Y	163_D	0.507	1.00
340_Y	343_I	0.506	1.00
81_V	234_Q	0.503	1.00
217_R	221_E	0.503	1.00
331_I	334_V	0.502	1.00
202_A	238_V	0.501	1.00
340_Y	354_E	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3gb0A	2	1	100	0.196	Contact Map
3rzaA	2	0.9919	100	0.21	Contact Map
3pfoA	2	0.9811	100	0.246	Contact Map
3ifeA	2	0.9704	100	0.265	Contact Map
1cg2A	4	0.973	100	0.278	Contact Map
4pqaA	2	0.9461	100	0.283	Contact Map
3iszA	2	0.9353	100	0.284	Contact Map
1fnoA	2	0.9488	100	0.286	Contact Map
2qyvA	2	0.9353	100	0.29	Contact Map
4mmoA	2	0.9515	100	0.292	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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