ARTQ - BACSU - GREMLIN Contacts

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ARTQ - Arginine transport system permease protein ArtQ

UniProt:	P54536	Sequences: MNLDFSATIPQIPFILEGLAITLKIVVVSAIIGLILGIVLSLCKISTFRPFIWIADFYTSVFRGTPLVLQLMIVYFGLPQLLGFQIDQFWAAVVALSLNSAAYVSEIIRAGINAIDKGQKEAAVALGVPYGKMMKDLLLPQAFKNISPAIVNELITLTKESAIVTVIGLGDVMRRAYQAGAATYNYLEPLIIAGLIYYVLVLILTFIGKAVERKLKSND	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	219 (206)
Sequences:	19470 (11687)
Seq/√Len:	814.3

ECOLI - HISM GLTJ ARTQ HISQ GLTK YHDY YECS GLNP ARTM YHDX YEHY METI PROW CYSW CYST THIP YEHW TAUC YDCV POTB POTI POTH PHNE POTC PSTC SSUC PSTA YDCU MODB YCJO YCJP UGPA UGPE MALG
BACSU - ARTQ TCYM GLNM GLNP YCKA YXEN TCYL TCYB METP OPUCB OPUBB OPUBD OPUCD OPUAB MDXF YTLD SSUC ARAP GANP YTEP YQGI YQGH AMYD AMYC YURN LPLB YURM MDXG GANQ ARAQ YESQ YVGM YESP
HALSA - Q9HML6 Q9HS11 Q9HS12 Q9HNI9 Q9HML7 Q9HP02 Q9HP01 Q9HPK3 Q9HMT4 Q9HR05 Q9HHQ6 Q9HHQ7
SULSO - Q97UZ0 Q97YY3 Q97UF3 Q97ZC2 Q97UY9 Q97YY2 Q97ZC1 Q97Z98 Q97UF4 Q97ZT6 Q97ZT7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
151_V	208_G	3.252	1.00
32_I	97_S	3.250	1.00
123_A	133_M	3.167	1.00
185_N	188_E	3.087	1.00
92_A	164_V	2.957	1.00
20_A	24_K	2.787	1.00
129_P	132_K	2.734	1.00
176_A	193_A	2.571	1.00
43_C	55_A	2.562	1.00
131_G	135_K	2.265	1.00
124_V	133_M	2.262	1.00
40_L	105_S	2.215	1.00
206_F	210_A	2.203	1.00
120_K	133_M	2.201	1.00
65_T	70_Q	2.149	1.00
119_Q	141_Q	2.097	1.00
50_P	54_I	2.023	1.00
120_K	124_V	1.997	1.00
28_V	93_V	1.957	1.00
174_R	178_Q	1.929	1.00
50_P	53_W	1.891	1.00
40_L	101_A	1.889	1.00
44_K	56_D	1.886	1.00
36_L	101_A	1.854	1.00
49_R	53_W	1.827	1.00
21_I	24_K	1.818	1.00
41_S	108_I	1.789	1.00
87_D	90_W	1.735	1.00
16_L	20_A	1.703	1.00
24_K	89_F	1.697	1.00
202_L	206_F	1.694	1.00
114_A	145_N	1.658	1.00
210_A	213_R	1.658	1.00
45_I	113_N	1.644	1.00
19_L	200_L	1.628	1.00
17_E	20_A	1.620	1.00
30_A	154_L	1.591	1.00
132_K	136_D	1.591	1.00
74_V	95_A	1.582	1.00
21_I	169_L	1.580	1.00
206_F	209_K	1.565	1.00
25_I	164_V	1.556	1.00
198_Y	202_L	1.552	1.00
129_P	133_M	1.551	1.00
176_A	189_P	1.542	1.00
26_V	158_T	1.538	1.00
111_G	146_I	1.523	1.00
14_F	17_E	1.518	1.00
130_Y	134_M	1.492	1.00
59_T	105_S	1.485	1.00
33_G	100_S	1.484	1.00
180_G	186_Y	1.480	1.00
106_E	109_R	1.467	1.00
38_I	108_I	1.447	1.00
104_V	150_I	1.407	1.00
40_L	55_A	1.396	1.00
128_V	132_K	1.389	1.00
124_V	130_Y	1.374	1.00
207_I	210_A	1.359	1.00
175_R	178_Q	1.298	1.00
70_Q	99_N	1.287	1.00
132_K	135_K	1.279	1.00
104_V	153_E	1.278	1.00
48_F	51_F	1.275	1.00
28_V	97_S	1.259	1.00
26_V	161_S	1.258	1.00
108_I	146_I	1.249	1.00
49_R	52_I	1.248	1.00
48_F	53_W	1.240	1.00
12_I	16_L	1.226	1.00
174_R	177_Y	1.224	1.00
109_R	113_N	1.218	1.00
39_V	43_C	1.213	1.00
24_K	28_V	1.212	1.00
14_F	175_R	1.205	1.00
52_I	56_D	1.205	1.00
35_I	39_V	1.204	1.00
32_I	93_V	1.178	1.00
170_G	174_R	1.176	1.00
89_F	93_V	1.176	1.00
120_K	138_L	1.174	1.00
53_W	57_F	1.166	1.00
37_G	104_V	1.143	1.00
123_A	137_L	1.132	1.00
62_F	98_L	1.132	1.00
210_A	214_K	1.129	1.00
71_L	95_A	1.126	1.00
90_W	93_V	1.122	1.00
17_E	21_I	1.118	1.00
54_I	57_F	1.117	1.00
130_Y	133_M	1.104	1.00
178_Q	182_A	1.102	1.00
65_T	99_N	1.099	1.00
112_I	138_L	1.097	1.00
107_I	153_E	1.096	1.00
93_V	97_S	1.096	1.00
13_P	17_E	1.092	1.00
60_S	202_L	1.091	1.00
71_L	164_V	1.087	1.00
177_Y	180_G	1.054	1.00
88_Q	168_G	1.049	1.00
43_C	46_S	1.043	1.00
180_G	190_L	1.037	1.00
26_V	154_L	1.026	1.00
134_M	139_L	1.025	1.00
45_I	112_I	1.014	1.00
54_I	58_Y	1.009	1.00
128_V	136_D	1.008	1.00
44_K	106_E	1.005	1.00
158_T	200_L	1.002	1.00
55_A	105_S	0.995	1.00
115_I	142_A	0.987	1.00
70_Q	74_V	0.979	1.00
30_A	157_L	0.966	1.00
207_I	211_V	0.951	1.00
209_K	213_R	0.948	1.00
89_F	169_L	0.938	1.00
29_S	97_S	0.935	1.00
62_F	102_A	0.935	1.00
107_I	149_A	0.934	1.00
117_K	121_E	0.921	1.00
47_T	52_I	0.920	1.00
188_E	191_I	0.919	1.00
34_L	150_I	0.910	1.00
19_L	196_I	0.907	1.00
57_F	60_S	0.902	1.00
156_T	160_E	0.901	1.00
67_L	160_E	0.897	1.00
74_V	91_A	0.896	1.00
43_C	51_F	0.888	1.00
158_T	204_L	0.888	1.00
134_M	138_L	0.874	1.00
164_V	171_D	0.873	1.00
31_I	35_I	0.872	1.00
70_Q	95_A	0.872	1.00
90_W	94_V	0.869	1.00
58_Y	101_A	0.869	1.00
209_K	212_E	0.867	1.00
166_V	170_G	0.861	1.00
22_T	158_T	0.859	1.00
60_S	198_Y	0.857	1.00
28_V	32_I	0.853	1.00
41_S	112_I	0.852	1.00
71_L	163_I	0.851	1.00
48_F	54_I	0.847	1.00
162_A	197_Y	0.847	1.00
53_W	56_D	0.842	1.00
100_S	153_E	0.841	1.00
176_A	180_G	0.841	1.00
183_T	186_Y	0.838	1.00
203_I	207_I	0.832	1.00
123_A	128_V	0.830	1.00
115_I	145_N	0.828	1.00
29_S	157_L	0.828	1.00
18_G	175_R	0.827	1.00
67_L	99_N	0.826	1.00
42_L	134_M	0.824	1.00
48_F	52_I	0.822	1.00
145_N	149_A	0.822	1.00
179_A	189_P	0.821	1.00
41_S	45_I	0.821	1.00
120_K	130_Y	0.799	1.00
182_A	185_N	0.799	1.00
73_I	77_G	0.796	1.00
164_V	169_L	0.796	1.00
58_Y	62_F	0.794	1.00
96_L	161_S	0.790	1.00
29_S	96_L	0.790	1.00
88_Q	169_L	0.787	1.00
112_I	142_A	0.785	1.00
199_V	203_I	0.774	1.00
118_G	122_A	0.771	1.00
165_T	174_R	0.763	1.00
13_P	16_L	0.763	1.00
19_L	172_V	0.763	1.00
170_G	175_R	0.761	1.00
46_S	130_Y	0.757	1.00
38_I	139_L	0.756	1.00
56_D	60_S	0.751	1.00
33_G	104_V	0.746	1.00
27_V	31_I	0.746	1.00
75_Y	91_A	0.744	1.00
191_I	195_L	0.743	1.00
46_S	55_A	0.738	1.00
40_L	43_C	0.738	1.00
117_K	120_K	0.737	1.00
104_V	108_I	0.735	1.00
108_I	112_I	0.733	1.00
71_L	167_I	0.733	1.00
100_S	160_E	0.728	1.00
158_T	201_V	0.727	1.00
73_I	191_I	0.726	1.00
68_V	166_V	0.726	1.00
36_L	97_S	0.726	1.00
111_G	145_N	0.722	1.00
25_I	29_S	0.718	1.00
204_L	208_G	0.712	1.00
92_A	167_I	0.711	1.00
51_F	54_I	0.710	1.00
21_I	89_F	0.706	1.00
86_I	90_W	0.703	1.00
25_I	92_A	0.702	1.00
58_Y	102_A	0.701	1.00
205_T	209_K	0.695	1.00
192_I	196_I	0.694	1.00
23_L	27_V	0.691	1.00
44_K	47_T	0.690	1.00
100_S	157_L	0.688	1.00
195_L	199_V	0.677	1.00
44_K	59_T	0.673	1.00
72_M	76_F	0.671	1.00
149_A	152_N	0.670	1.00
28_V	31_I	0.659	1.00
180_G	189_P	0.657	1.00
162_A	165_T	0.657	1.00
75_Y	167_I	0.656	1.00
44_K	52_I	0.656	1.00
99_N	160_E	0.655	1.00
190_L	193_A	0.654	1.00
77_G	80_Q	0.652	1.00
100_S	104_V	0.651	1.00
25_I	169_L	0.650	1.00
36_L	40_L	0.649	1.00
32_I	35_I	0.648	1.00
22_T	197_Y	0.647	1.00
26_V	30_A	0.641	1.00
74_V	79_P	0.632	1.00
118_G	121_E	0.626	1.00
61_V	65_T	0.626	1.00
74_V	78_L	0.625	1.00
20_A	23_L	0.625	1.00
211_V	215_L	0.623	1.00
96_L	157_L	0.620	1.00
191_I	194_G	0.618	1.00
173_M	193_A	0.618	1.00
88_Q	91_A	0.616	1.00
11_Q	179_A	0.615	1.00
28_V	89_F	0.615	1.00
153_E	156_T	0.614	1.00
43_C	54_I	0.614	1.00
172_V	175_R	0.610	1.00
62_F	70_Q	0.604	1.00
97_S	101_A	0.600	1.00
16_L	19_L	0.600	1.00
138_L	141_Q	0.598	1.00
129_P	136_D	0.596	1.00
21_I	171_D	0.595	1.00
92_A	169_L	0.595	1.00
22_T	26_V	0.594	1.00
147_S	215_L	0.592	1.00
115_I	138_L	0.591	1.00
47_T	50_P	0.591	1.00
37_G	108_I	0.590	1.00
117_K	124_V	0.589	1.00
88_Q	167_I	0.588	1.00
34_L	108_I	0.586	1.00
148_P	212_E	0.585	1.00
77_G	81_L	0.581	1.00
173_M	197_Y	0.577	1.00
173_M	190_L	0.576	1.00
32_I	36_L	0.574	1.00
38_I	143_F	0.571	1.00
193_A	196_I	0.569	1.00
198_Y	201_V	0.568	1.00
64_G	99_N	0.568	1.00
15_I	196_I	0.565	1.00
37_G	105_S	0.563	1.00
202_L	205_T	0.563	1.00
75_Y	88_Q	0.562	1.00
172_V	196_I	0.561	1.00
35_I	97_S	0.560	1.00
30_A	34_L	0.557	1.00
24_K	90_W	0.556	1.00
47_T	130_Y	0.552	1.00
187_L	191_I	0.552	1.00
176_A	179_A	0.550	1.00
25_I	89_F	0.548	1.00
112_I	134_M	0.548	1.00
74_V	77_G	0.545	1.00
208_G	211_V	0.545	1.00
116_D	145_N	0.544	1.00
144_K	215_L	0.544	1.00
96_L	164_V	0.543	1.00
147_S	211_V	0.543	1.00
11_Q	14_F	0.539	1.00
57_F	61_V	0.538	1.00
203_I	210_A	0.537	1.00
44_K	105_S	0.537	1.00
179_A	183_T	0.536	1.00
190_L	194_G	0.535	1.00
35_I	38_I	0.529	1.00
47_T	51_F	0.529	1.00
121_E	136_D	0.527	1.00
15_I	176_A	0.527	1.00
40_L	58_Y	0.527	1.00
37_G	41_S	0.522	1.00
123_A	126_L	0.519	1.00
72_M	166_V	0.518	1.00
176_A	190_L	0.517	1.00
36_L	39_V	0.515	1.00
56_D	59_T	0.513	1.00
203_I	206_F	0.513	1.00
94_V	97_S	0.512	1.00
154_L	204_L	0.511	1.00
128_V	133_M	0.510	1.00
87_D	91_A	0.508	1.00
120_K	134_M	0.506	1.00
111_G	149_A	0.504	1.00
21_I	25_I	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4ymuD	2	0.9817	100	0.372	Contact Map
2onkC	2	0.9589	100	0.443	Contact Map
3tuiA	2	0.9543	100	0.446	Contact Map
3d31C	2	0.9452	100	0.459	Contact Map
3rlfF	1	0.9406	99.9	0.461	Contact Map
4tquM	1	0.9863	99.9	0.481	Contact Map
3rlfG	1	0.9954	99.9	0.5	Contact Map
4tquN	1	0.9635	99.9	0.543	Contact Map
3w9iA	3	0.7854	12.3	0.944	Contact Map
3ne5A	3	0.8402	10.4	0.946	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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