ARTP - BACSU - GREMLIN Contacts

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ARTP - Arginine-binding extracellular protein ArtP

UniProt:	P54535	Sequences: MKKWLLLLVAACITFALTACGSSNSGSESGKKKLIMGTSADYKPFEYKEGDNIVGFDVELAKALAKKAGYEIEVQDMDFNSLITALKSKQVDLVLSGMTPTPERKKQVDFSDVYYTANHMIVSKKDSGIQSLKDLKGKTVGVQLGSIQEEKGKELSPEYGFKTEDRNRISDLVQEIKSDRFDAAIIEDIVAEGYFKSNDDLQGFVIPDAKAEEAGSAIAFRKDSELTDKFNKALKEMEDNGELEKLKKKWFTGEK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	255 (238)
Sequences:	22449 (16388)
Seq/√Len:	1062.3

ECOLI - GLTI YHDW GLNH HISJ FLIY ARGT ARTJ ARTI MLTF EVGS
BACSU - ARTP GLNH TCYJ YCKB YXEM TCYK TCYA

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
37_G	91_V	3.855	1.00
141_G	181_F	3.651	1.00
33_K	73_E	3.586	1.00
67_K	236_E	2.962	1.00
149_E	165_D	2.426	1.00
166_R	175_E	2.414	1.00
49_E	54_V	2.386	1.00
85_A	91_V	2.336	1.00
112_D	235_K	2.281	1.00
168_R	171_D	2.279	1.00
35_I	73_E	2.248	1.00
100_P	217_A	2.247	1.00
100_P	105_K	2.191	1.00
102_P	105_K	2.170	1.00
149_E	153_K	2.146	1.00
35_I	75_Q	2.128	1.00
242_E	245_K	2.127	1.00
244_E	248_K	2.116	1.00
87_K	107_Q	2.065	1.00
33_K	71_E	2.043	1.00
63_A	66_K	2.040	1.00
65_A	72_I	2.013	1.00
235_K	239_D	2.011	1.00
139_T	164_E	2.008	1.00
108_V	219_A	1.975	1.00
227_T	231_N	1.927	1.00
67_K	233_A	1.914	1.00
109_D	227_T	1.904	1.00
220_F	227_T	1.903	1.00
186_I	191_A	1.877	1.00
220_F	230_F	1.853	1.00
112_D	231_N	1.852	1.00
131_S	134_D	1.841	1.00
172_L	184_A	1.825	1.00
224_S	228_D	1.759	1.00
63_A	242_E	1.759	1.00
122_V	191_A	1.722	1.00
36_M	61_A	1.709	1.00
95_L	218_I	1.695	1.00
130_Q	134_D	1.694	1.00
175_E	180_R	1.684	1.00
139_T	162_K	1.680	1.00
144_L	167_N	1.653	1.00
85_A	90_Q	1.637	1.00
63_A	67_K	1.612	1.00
124_K	127_S	1.573	1.00
111_S	231_N	1.571	1.00
63_A	237_M	1.570	1.00
49_E	52_N	1.537	1.00
124_K	179_D	1.519	1.00
228_D	232_K	1.503	1.00
77_M	85_A	1.458	1.00
67_K	240_N	1.444	1.00
82_L	94_V	1.426	1.00
59_E	249_K	1.388	1.00
229_K	232_K	1.387	1.00
116_T	215_G	1.384	1.00
83_I	108_V	1.382	1.00
150_E	154_E	1.378	1.00
47_Y	54_V	1.362	1.00
167_N	171_D	1.360	1.00
62_K	74_V	1.349	1.00
139_T	182_D	1.347	1.00
26_G	30_G	1.347	1.00
175_E	181_F	1.342	1.00
64_L	233_A	1.333	1.00
188_D	192_E	1.318	1.00
58_V	62_K	1.306	1.00
138_K	182_D	1.293	1.00
126_D	205_V	1.290	1.00
89_K	221_R	1.280	1.00
36_M	93_L	1.277	1.00
68_A	233_A	1.275	1.00
98_M	219_A	1.274	1.00
153_K	165_D	1.268	1.00
48_K	76_D	1.250	1.00
120_M	188_D	1.247	1.00
119_H	148_Q	1.243	1.00
61_A	95_L	1.243	1.00
151_K	155_L	1.242	1.00
57_D	114_Y	1.238	1.00
141_G	166_R	1.236	1.00
40_A	76_D	1.227	1.00
123_S	183_A	1.214	1.00
56_F	247_K	1.207	1.00
121_I	183_A	1.202	1.00
236_E	240_N	1.191	1.00
94_V	98_M	1.183	1.00
142_V	149_E	1.176	1.00
84_T	87_K	1.176	1.00
140_V	183_A	1.163	1.00
17_L	20_C	1.161	1.00
56_F	246_L	1.157	1.00
103_E	106_K	1.155	1.00
174_Q	177_K	1.139	1.00
233_A	237_M	1.139	1.00
84_T	88_S	1.131	1.00
238_E	243_L	1.129	1.00
233_A	236_E	1.127	1.00
77_M	91_V	1.119	1.00
26_G	29_S	1.112	1.00
229_K	233_A	1.111	1.00
27_S	31_K	1.111	1.00
166_R	172_L	1.111	1.00
224_S	227_T	1.111	1.00
109_D	224_S	1.105	1.00
32_K	70_Y	1.102	1.00
174_Q	178_S	1.099	1.00
176_I	204_F	1.096	1.00
62_K	66_K	1.086	1.00
176_I	184_A	1.077	1.00
78_D	81_S	1.076	1.00
61_A	74_V	1.065	1.00
25_S	28_E	1.057	1.00
120_M	206_I	1.046	1.00
125_K	202_Q	1.039	1.00
70_Y	229_K	1.038	1.00
176_I	181_F	1.037	1.00
52_N	62_K	1.033	1.00
232_K	236_E	1.033	1.00
60_L	237_M	1.027	1.00
61_A	72_I	1.026	1.00
109_D	222_K	1.014	1.00
36_M	72_I	1.012	1.00
54_V	59_E	1.010	1.00
225_E	229_K	1.010	1.00
31_K	35_I	1.009	1.00
60_L	243_L	1.006	1.00
123_S	207_P	1.003	1.00
143_Q	146_S	1.000	1.00
59_E	62_K	0.999	1.00
242_E	246_L	0.993	1.00
231_N	234_L	0.990	1.00
193_G	197_S	0.987	1.00
48_K	51_D	0.984	1.00
28_E	32_K	0.976	1.00
56_F	243_L	0.975	1.00
177_K	198_N	0.973	1.00
101_T	104_R	0.972	1.00
87_K	108_V	0.971	1.00
59_E	250_W	0.970	1.00
156_S	159_Y	0.965	1.00
144_L	165_D	0.964	1.00
150_E	153_K	0.961	1.00
93_L	220_F	0.961	1.00
141_G	172_L	0.960	1.00
64_L	230_F	0.953	1.00
171_D	174_Q	0.950	1.00
93_L	230_F	0.948	1.00
28_E	70_Y	0.946	1.00
123_S	127_S	0.944	1.00
67_K	242_E	0.938	1.00
237_M	242_E	0.930	1.00
53_I	58_V	0.926	1.00
83_I	98_M	0.926	1.00
243_L	247_K	0.913	1.00
245_K	248_K	0.911	1.00
28_E	31_K	0.903	1.00
133_K	136_K	0.892	1.00
102_P	106_K	0.891	1.00
117_A	189_I	0.890	1.00
170_S	174_Q	0.885	1.00
110_F	219_A	0.876	1.00
231_N	235_K	0.872	1.00
111_S	230_F	0.868	1.00
13_I	16_A	0.868	1.00
93_L	218_I	0.867	1.00
79_F	82_L	0.865	1.00
34_L	93_L	0.861	1.00
112_D	238_E	0.860	1.00
43_K	47_Y	0.858	1.00
81_S	85_A	0.858	1.00
45_F	115_Y	0.854	1.00
151_K	154_E	0.850	1.00
104_R	110_F	0.845	1.00
61_A	218_I	0.845	1.00
125_K	205_V	0.844	1.00
121_I	185_I	0.840	1.00
40_A	53_I	0.837	1.00
48_K	53_I	0.835	1.00
245_K	249_K	0.832	1.00
105_K	222_K	0.831	1.00
34_L	70_Y	0.824	1.00
236_E	239_D	0.819	1.00
94_V	219_A	0.818	1.00
25_S	29_S	0.811	1.00
38_T	58_V	0.807	1.00
63_A	233_A	0.792	1.00
164_E	175_E	0.792	1.00
99_T	214_A	0.789	1.00
64_L	68_A	0.787	1.00
169_I	186_I	0.785	1.00
118_N	188_D	0.783	1.00
198_N	201_L	0.781	1.00
38_T	74_V	0.780	1.00
29_S	32_K	0.779	1.00
68_A	229_K	0.775	1.00
100_P	113_V	0.774	1.00
237_M	240_N	0.772	1.00
12_C	15_F	0.772	1.00
16_A	19_A	0.771	1.00
61_A	93_L	0.770	1.00
109_D	223_D	0.769	1.00
177_K	200_D	0.764	1.00
75_Q	90_Q	0.759	1.00
36_M	74_V	0.759	1.00
155_L	159_Y	0.751	1.00
244_E	247_K	0.751	1.00
111_S	220_F	0.744	1.00
135_L	183_A	0.741	1.00
173_V	177_K	0.737	1.00
67_K	237_M	0.737	1.00
122_V	204_F	0.736	1.00
62_K	72_I	0.735	1.00
18_T	21_G	0.734	1.00
64_L	95_L	0.734	1.00
38_T	96_S	0.729	1.00
34_L	65_A	0.723	1.00
117_A	188_D	0.722	1.00
154_E	157_P	0.720	1.00
141_G	175_E	0.717	1.00
120_M	191_A	0.716	1.00
237_M	243_L	0.713	1.00
232_K	235_K	0.709	1.00
35_I	92_D	0.707	1.00
129_I	134_D	0.706	1.00
47_Y	250_W	0.704	1.00
86_L	94_V	0.701	1.00
142_V	165_D	0.698	1.00
10_A	14_T	0.698	1.00
39_S	79_F	0.696	1.00
99_T	104_R	0.695	1.00
218_I	230_F	0.686	1.00
171_D	175_E	0.685	1.00
86_L	91_V	0.684	1.00
166_R	181_F	0.684	1.00
83_I	87_K	0.682	1.00
20_C	23_S	0.681	1.00
40_A	46_E	0.680	1.00
63_A	246_L	0.679	1.00
39_S	46_E	0.677	1.00
237_M	241_G	0.676	1.00
192_E	206_I	0.676	1.00
60_L	234_L	0.674	1.00
192_E	196_K	0.673	1.00
54_V	250_W	0.671	1.00
81_S	84_T	0.669	1.00
225_E	228_D	0.666	1.00
16_A	20_C	0.662	1.00
193_G	196_K	0.661	1.00
42_Y	97_G	0.660	1.00
37_G	82_L	0.659	1.00
19_A	22_S	0.652	1.00
155_L	158_E	0.651	1.00
164_E	181_F	0.651	1.00
33_K	70_Y	0.646	1.00
235_K	238_E	0.643	1.00
42_Y	45_F	0.643	1.00
139_T	181_F	0.639	1.00
228_D	231_N	0.639	1.00
111_S	218_I	0.634	1.00
75_Q	91_V	0.634	1.00
13_I	17_L	0.632	1.00
100_P	110_F	0.631	1.00
21_G	24_N	0.631	1.00
17_L	21_G	0.630	1.00
152_G	156_S	0.627	1.00
122_V	173_V	0.627	1.00
44_P	47_Y	0.623	1.00
110_F	217_A	0.622	1.00
221_R	224_S	0.621	1.00
112_D	234_L	0.620	1.00
152_G	185_I	0.618	1.00
86_L	108_V	0.617	1.00
38_T	53_I	0.616	1.00
87_K	221_R	0.616	1.00
118_N	212_E	0.615	1.00
113_V	116_T	0.612	1.00
147_I	214_A	0.610	1.00
79_F	98_M	0.609	1.00
20_C	24_N	0.609	1.00
98_M	108_V	0.608	1.00
120_M	208_D	0.604	1.00
123_S	205_V	0.604	1.00
116_T	213_E	0.603	1.00
25_S	70_Y	0.602	1.00
65_A	68_A	0.601	1.00
153_K	163_T	0.600	1.00
77_M	82_L	0.597	1.00
152_G	163_T	0.597	1.00
42_Y	79_F	0.594	1.00
58_V	74_V	0.592	1.00
122_V	195_F	0.590	1.00
39_S	143_Q	0.589	1.00
46_E	96_S	0.588	1.00
37_G	94_V	0.587	1.00
66_K	242_E	0.586	1.00
241_G	245_K	0.584	1.00
119_H	185_I	0.583	1.00
118_N	213_E	0.583	1.00
65_A	70_Y	0.582	1.00
65_A	69_G	0.578	1.00
191_A	195_F	0.578	1.00
11_A	14_T	0.575	1.00
140_V	152_G	0.575	1.00
111_S	234_L	0.574	1.00
27_S	30_G	0.570	1.00
126_D	179_D	0.569	1.00
90_Q	180_R	0.568	1.00
86_L	221_R	0.568	1.00
25_S	31_K	0.566	1.00
39_S	96_S	0.565	1.00
57_D	97_G	0.565	1.00
64_L	218_I	0.564	1.00
186_I	190_V	0.563	1.00
61_A	65_A	0.562	1.00
128_G	138_K	0.561	1.00
136_K	158_E	0.558	1.00
181_F	184_A	0.555	1.00
118_N	210_K	0.555	1.00
31_K	71_E	0.555	1.00
115_Y	216_S	0.553	1.00
136_K	156_S	0.549	1.00
79_F	104_R	0.548	1.00
105_K	109_D	0.548	1.00
29_S	229_K	0.547	1.00
15_F	18_T	0.547	1.00
40_A	48_K	0.542	1.00
237_M	246_L	0.540	1.00
10_A	13_I	0.540	1.00
82_L	98_M	0.539	1.00
144_L	149_E	0.536	1.00
201_L	204_F	0.535	1.00
128_G	205_V	0.535	1.00
109_D	221_R	0.534	1.00
121_I	135_L	0.534	1.00
241_G	244_E	0.534	1.00
136_K	157_P	0.533	1.00
238_E	241_G	0.533	1.00
99_T	147_I	0.529	1.00
68_A	226_L	0.527	1.00
34_L	68_A	0.525	1.00
31_K	92_D	0.525	1.00
142_V	152_G	0.522	1.00
50_G	54_V	0.522	1.00
35_I	89_K	0.518	1.00
223_D	226_L	0.514	1.00
175_E	178_S	0.514	1.00
143_Q	169_I	0.514	1.00
11_A	15_F	0.512	1.00
32_K	229_K	0.511	1.00
195_F	199_D	0.510	1.00
82_L	85_A	0.509	1.00
136_K	159_Y	0.506	1.00
61_A	64_L	0.505	1.00
75_Q	85_A	0.501	1.00
56_F	250_W	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3kbrA	4	0.8863	100	0.254	Contact Map
1xt8A	1	0.8941	100	0.258	Contact Map
1pb7A	1	0.9412	100	0.263	Contact Map
2vhaA	2	0.9333	100	0.267	Contact Map
2ylnA	1	0.902	100	0.267	Contact Map
4ohnA	1	0.9176	100	0.268	Contact Map
3hv1A	2	0.9373	100	0.272	Contact Map
2yjpA	1	0.898	100	0.274	Contact Map
4powA	1	0.8706	100	0.274	Contact Map
2ieeA	1	0.8941	100	0.279	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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