GREMLIN Database
PRPB - Methylisocitrate lyase
UniProt: P54528 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 301 (261)
Sequences: 4106 (2200)
Seq/√Len: 136.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
53_S56_A3.5931.00
140_I180_A3.1481.00
43_K86_D2.8951.00
182_I209_V2.8501.00
202_Q206_R2.8221.00
78_A115_V2.7861.00
204_A211_L2.6701.00
197_E228_E2.5361.00
196_A199_E2.5201.00
201_R205_E2.4531.00
166_R199_E2.4191.00
27_I230_E2.4111.00
197_E232_M2.3661.00
173_A176_K2.3201.00
257_L261_H2.3041.00
228_E231_D2.2841.00
33_A42_A2.2831.00
225_R228_E2.2821.00
151_K154_A2.2111.00
158_I188_A2.2031.00
169_E173_A2.1991.00
256_G260_E2.1861.00
194_L203_F2.1841.00
93_I118_V2.0021.00
82_V89_L1.9761.00
253_R270_D1.9701.00
71_S109_E1.9531.00
139_P142_E1.9001.00
91_V110_M1.8911.00
44_E252_E1.8711.00
200_F213_A1.8651.00
17_R20_K1.8491.00
43_K87_L1.8381.00
144_A180_A1.8151.00
103_A150_I1.7931.00
33_A50_I1.7521.00
171_L175_I1.7461.00
182_I208_P1.7451.00
16_G156_S1.7111.00
98_G102_N1.7001.00
140_I144_A1.6851.00
172_D202_Q1.6721.00
249_K253_R1.6691.00
42_A255_F1.6251.00
26_D230_E1.6101.00
25_P235_H1.5911.00
30_I49_A1.5781.00
175_I179_E1.5751.00
227_D231_D1.5751.00
172_D176_K1.5551.00
90_L160_V1.5531.00
243_S255_F1.5481.00
198_N201_R1.5421.00
117_A160_V1.5281.00
171_L202_Q1.5121.00
27_I237_V1.5021.00
178_S189_I1.5011.00
252_E256_G1.4991.00
140_I181_Y1.4941.00
85_A89_L1.4881.00
107_A150_I1.4831.00
91_V115_V1.4751.00
107_A118_V1.4601.00
47_F259_K1.4531.00
179_E206_R1.4461.00
213_A229_F1.4451.00
227_D230_E1.4391.00
16_G20_K1.4321.00
178_S207_I1.4311.00
165_A174_A1.4281.00
167_A170_G1.4271.00
261_H266_E1.4111.00
204_A234_F1.4071.00
143_M181_Y1.4001.00
178_S191_P1.3851.00
70_T73_E1.3781.00
140_I176_K1.3621.00
117_A158_I1.3591.00
124_Q136_Q1.3351.00
93_I110_M1.3301.00
41_L255_F1.3251.00
147_I186_A1.3101.00
138_V142_E1.3051.00
197_E201_R1.3041.00
247_A250_A1.2651.00
61_R80_D1.2601.00
38_A251_C1.2551.00
42_A50_I1.2541.00
142_E145_Q1.2441.00
118_V157_L1.2421.00
224_Y232_M1.2361.00
74_I110_M1.2241.00
20_K23_S1.2211.00
48_S259_K1.2211.00
246_A254_M1.2161.00
151_K159_V1.2121.00
110_M118_V1.2091.00
21_L48_S1.2051.00
24_A235_H1.2011.00
189_I209_V1.1981.00
111_L157_L1.1981.00
78_A91_V1.1951.00
175_I207_I1.1861.00
74_I97_F1.1610.99
24_A48_S1.1520.99
179_E183_E1.1390.99
138_V146_K1.1340.99
55_A59_A1.1310.99
191_P203_F1.1250.99
51_Y119_Q1.1040.99
57_Y77_R1.0960.99
176_K179_E1.0890.99
50_I85_A1.0870.99
76_E79_K1.0790.99
211_L234_F1.0670.99
18_F158_I1.0660.99
29_Q226_A1.0620.99
203_F207_I1.0610.99
40_L44_E1.0600.99
161_A186_A1.0590.99
111_L154_A1.0560.99
217_E242_T1.0550.99
247_A251_C1.0540.99
246_A250_A1.0490.99
212_L238_I1.0450.99
93_I106_T1.0350.99
176_K180_A1.0260.99
30_I90_L1.0260.99
19_R158_I1.0250.99
19_R187_D1.0170.98
195_Q199_E1.0140.98
30_I236_M1.0120.98
204_A235_H1.0110.98
71_S106_T1.0030.98
147_I184_A0.9940.98
201_R232_M0.9910.98
83_R112_E0.9890.98
257_L263_S0.9880.98
189_I207_I0.9830.98
45_A256_G0.9780.98
144_A184_A0.9770.98
34_H38_A0.9640.98
166_R171_L0.9610.98
105_R109_E0.9540.98
143_M146_K0.9530.98
253_R256_G0.9490.98
72_A76_E0.9480.98
95_T138_V0.9440.97
37_M244_L0.9370.97
171_L206_R0.9360.97
35_D38_A0.9330.97
120_M150_I0.9290.97
52_L78_A0.9200.97
31_P47_F0.9180.97
226_A237_V0.9170.97
99_G102_N0.9160.97
54_G94_D0.9100.97
182_I207_I0.9090.97
179_E208_P0.9080.97
51_Y238_I0.9070.97
119_Q160_V0.9030.97
40_L43_K0.8970.97
23_S26_D0.8920.96
25_P230_E0.8910.96
165_A173_A0.8870.96
77_R80_D0.8850.96
199_E202_Q0.8830.96
144_A181_Y0.8820.96
179_E207_I0.8810.96
160_V188_A0.8790.96
59_A244_L0.8780.96
163_T178_S0.8770.96
246_A271_M0.8750.96
250_A254_M0.8730.96
119_Q164_D0.8670.96
111_L155_P0.8620.96
63_L70_T0.8600.96
17_R21_L0.8570.96
55_A241_V0.8560.96
15_A116_A0.8560.96
98_G106_T0.8530.95
30_I160_V0.8520.95
52_L89_L0.8510.95
192_E214_N0.8450.95
79_K112_E0.8410.95
64_P68_I0.8400.95
63_L73_E0.8380.95
14_L17_R0.8370.95
255_F259_K0.8340.95
166_R174_A0.8330.95
180_A183_E0.8320.95
57_Y78_A0.8280.95
42_A243_S0.8240.95
243_S251_C0.8220.94
20_K24_A0.8180.94
90_L236_M0.8150.94
143_M147_I0.8150.94
182_I189_I0.8100.94
53_S241_V0.8090.94
100_V142_E0.8080.94
19_R156_S0.8040.94
204_A232_M0.8030.94
188_A236_M0.7980.94
174_A203_F0.7960.93
41_L45_A0.7960.93
258_M264_Q0.7960.93
273_T276_E0.7940.93
35_D60_S0.7910.93
140_I143_M0.7870.93
201_R228_E0.7850.93
19_R23_S0.7820.93
32_G51_Y0.7800.93
32_G238_I0.7770.93
200_F232_M0.7760.93
67_G95_T0.7750.93
202_Q205_E0.7740.92
28_L49_A0.7730.92
241_V244_L0.7710.92
78_A110_M0.7670.92
144_A148_K0.7660.92
83_R109_E0.7660.92
137_L165_A0.7630.92
141_K145_Q0.7600.92
148_K152_Q0.7550.92
197_E224_Y0.7540.91
190_F214_N0.7530.91
39_A85_A0.7520.91
154_A159_V0.7510.91
52_L57_Y0.7500.91
116_A156_S0.7500.91
78_A89_L0.7490.91
72_A109_E0.7490.91
109_E112_E0.7480.91
131_H214_N0.7450.91
168_Q173_A0.7430.91
179_E209_V0.7400.91
249_K252_E0.7380.91
228_E232_M0.7360.90
79_K113_A0.7350.90
252_E276_E0.7350.90
24_A27_I0.7320.90
77_R81_L0.7310.90
116_A155_P0.7300.90
74_I91_V0.7300.90
163_T191_P0.7270.90
106_T110_M0.7230.90
175_I206_R0.7230.90
151_K187_D0.7210.90
198_N260_E0.7210.90
200_F234_F0.7190.89
43_K85_A0.7160.89
79_K83_R0.7140.89
245_R277_L0.7120.89
160_V236_M0.7100.89
21_L24_A0.7060.89
267_G270_D0.7050.88
59_A277_L0.7040.88
52_L81_L0.7030.88
93_I107_A0.7030.88
103_A146_K0.7010.88
181_Y184_A0.7000.88
178_S203_F0.6990.88
104_A152_Q0.6930.88
99_G145_Q0.6920.88
99_G126_P0.6910.87
242_T254_M0.6860.87
15_A111_L0.6860.87
214_N241_V0.6850.87
79_K109_E0.6840.87
74_I78_A0.6790.87
253_R257_L0.6790.87
55_A245_R0.6760.86
167_A193_A0.6760.86
13_E16_G0.6710.86
242_T264_Q0.6710.86
219_G222_P0.6670.86
147_I151_K0.6660.86
140_I173_A0.6640.85
18_F22_M0.6630.85
41_L248_A0.6610.85
40_L84_A0.6610.85
99_G142_E0.6600.85
37_M248_A0.6600.85
104_A149_A0.6590.85
69_I74_I0.6580.85
172_D206_R0.6540.84
82_V116_A0.6470.84
171_L199_E0.6460.84
165_A170_G0.6460.84
36_G84_A0.6460.84
61_R77_R0.6440.84
248_A272_Q0.6430.83
150_I153_A0.6420.83
100_V145_Q0.6390.83
128_K132_L0.6350.83
75_A109_E0.6330.83
26_D235_H0.6330.83
36_G80_D0.6280.82
271_M274_R0.6260.82
239_Y243_S0.6250.82
226_A230_E0.6240.82
81_L89_L0.6230.82
174_A191_P0.6230.82
180_A184_A0.6210.81
135_K193_A0.6200.81
82_V115_V0.6190.81
131_H135_K0.6180.81
16_G19_R0.6160.81
224_Y229_F0.6150.81
117_A188_A0.6130.80
26_D48_S0.6130.80
100_V149_A0.6080.80
107_A111_L0.6070.80
110_M115_V0.6070.80
103_A120_M0.6030.79
149_A152_Q0.5960.79
123_Q129_C0.5940.78
56_A244_L0.5930.78
145_Q148_K0.5930.78
131_H192_E0.5920.78
130_G214_N0.5910.78
188_A209_V0.5880.78
108_R112_E0.5840.77
49_A88_P0.5830.77
57_Y74_I0.5800.77
148_K151_K0.5790.77
39_A81_L0.5760.76
15_A156_S0.5760.76
46_G87_L0.5750.76
18_F30_I0.5730.76
21_L26_D0.5720.76
82_V113_A0.5710.76
21_L28_L0.5700.76
225_R263_S0.5670.75
61_R76_E0.5670.75
249_K276_E0.5650.75
107_A154_A0.5640.75
71_S110_M0.5640.75
103_A149_A0.5620.74
179_E182_I0.5610.74
18_F28_L0.5610.74
71_S75_A0.5570.74
60_S77_R0.5570.74
166_R195_Q0.5570.74
154_A157_L0.5560.74
119_Q238_I0.5550.74
257_L266_E0.5550.74
132_L240_P0.5530.73
19_R22_M0.5520.73
223_Y229_F0.5500.73
47_F255_F0.5500.73
149_A153_A0.5480.73
55_A244_L0.5480.73
137_L140_I0.5470.73
29_Q239_Y0.5460.72
100_V104_A0.5450.72
36_G81_L0.5430.72
76_E80_D0.5430.72
95_T125_L0.5420.72
212_L236_M0.5410.72
25_P48_S0.5410.72
93_I120_M0.5400.72
15_A158_I0.5400.72
36_G77_R0.5390.71
130_G135_K0.5340.71
29_Q259_K0.5320.70
125_L135_K0.5300.70
176_K205_E0.5300.70
130_G192_E0.5260.70
119_Q188_A0.5260.70
22_M236_M0.5250.69
147_I159_V0.5240.69
272_Q277_L0.5240.69
32_G53_S0.5220.69
135_K214_N0.5210.69
49_A238_I0.5190.69
263_S266_E0.5190.69
58_T69_I0.5170.68
15_A155_P0.5160.68
142_E147_I0.5150.68
132_L277_L0.5130.68
241_V245_R0.5120.68
274_R277_L0.5110.67
148_K176_K0.5110.67
215_M223_Y0.5100.67
44_E108_R0.5080.67
250_A253_R0.5070.67
123_Q164_D0.5070.67
95_T142_E0.5050.66
213_A234_F0.5040.66
109_E113_A0.5030.66
123_Q163_T0.5000.66
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3fa4A 5 0.9535 100 0.214 Contact Map
3lyeA 4 0.9169 100 0.216 Contact Map
1s2wA 2 0.8306 100 0.217 Contact Map
2hjpA 4 0.9169 100 0.22 Contact Map
3eooA 4 0.9568 100 0.221 Contact Map
4iqdA 4 0.9668 100 0.226 Contact Map
1zlpA 4 0.9336 100 0.228 Contact Map
1xg4A 4 0.9402 100 0.23 Contact Map
1f8mA 4 0.99 100 0.239 Contact Map
3eolA 4 0.8837 100 0.248 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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