YQHQ - BACSU - GREMLIN Contacts

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YQHQ - Uncharacterized protein YqhQ

UniProt:	P54515	Sequences: MSKHKVPPAYGGQAVVEGVMFGGKHHYVTAIRRTDGSIDFFKLPRKHNPKLNIVKKIPFLRGIAAIIEASANGTKHLNFSSERYGLDPSEDETLEQEEKKSSGLSMYLSLAVIGVLSFLFSKFVFTLVPVFLAELTRPIFSLNTAQIAIESLFKLILLLGYIYFLSMTPLIKRVFQYHGAEHKVINCYEQNLPITVENVQNQSRLHYRCGSSFILFTIIVGMFVYLLVPTDPLWLRVIDRVALIPVVLGISFEVLQLTNKVRDIPGLKLLGYPGLWLQLLTTKEPKDEQVEVAIESFNELLRLEALSEQNQKPSHNVI	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	318 (291)
Sequences:	735 (540)
Seq/√Len:	31.6

BACSU - YQHQ

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
200_Q	287_D	7.266	1.00
192_L	202_Q	6.262	1.00
199_V	290_V	5.984	1.00
200_Q	290_V	5.453	1.00
224_V	250_I	4.944	1.00
196_V	287_D	3.672	1.00
192_L	201_N	3.330	1.00
257_L	261_V	3.125	1.00
118_F	122_K	3.101	1.00
19_V	296_S	3.054	1.00
30_A	38_I	2.887	1.00
176_Q	289_Q	2.740	1.00
111_A	115_V	2.572	1.00
154_K	240_R	2.358	0.99
254_V	270_L	2.355	0.99
264_I	267_L	2.301	0.99
223_F	227_L	2.287	0.99
192_L	198_N	2.267	0.99
59_F	246_V	2.199	0.99
26_H	42_K	2.034	0.98
183_K	284_E	2.005	0.98
169_P	172_K	1.973	0.97
215_L	277_L	1.973	0.97
196_V	290_V	1.971	0.97
41_F	79_F	1.967	0.97
59_F	63_I	1.956	0.97
216_F	254_V	1.952	0.97
215_L	219_I	1.934	0.97
199_V	294_I	1.915	0.97
59_F	250_I	1.910	0.97
255_L	258_T	1.897	0.97
158_L	218_I	1.861	0.96
205_L	279_L	1.857	0.96
216_F	270_L	1.843	0.96
187_C	198_N	1.826	0.96
252_F	256_Q	1.816	0.96
65_A	252_F	1.800	0.95
47_H	68_E	1.788	0.95
184_V	294_I	1.770	0.95
272_Y	276_W	1.718	0.94
187_C	199_V	1.712	0.94
190_Q	202_Q	1.705	0.94
204_R	279_L	1.693	0.94
13_Q	73_G	1.674	0.93
79_F	83_R	1.672	0.93
186_N	199_V	1.648	0.93
15_V	29_T	1.639	0.92
295_E	298_N	1.634	0.92
108_L	112_V	1.630	0.92
286_K	290_V	1.608	0.92
265_P	269_L	1.607	0.92
193_P	298_N	1.605	0.92
243_L	246_V	1.593	0.91
127_L	237_V	1.584	0.91
64_A	68_E	1.555	0.90
52_N	55_K	1.545	0.90
43_L	75_K	1.536	0.90
163_Y	167_M	1.533	0.90
119_L	123_F	1.531	0.89
204_R	284_E	1.519	0.89
214_I	218_I	1.512	0.89
113_I	117_S	1.510	0.89
130_V	236_R	1.506	0.89
186_N	207_Y	1.494	0.88
11_G	14_A	1.457	0.87
238_I	242_A	1.455	0.87
171_I	174_V	1.450	0.87
54_V	60_L	1.433	0.86
124_V	268_K	1.429	0.86
186_N	205_L	1.426	0.86
19_V	28_V	1.418	0.85
261_V	264_I	1.416	0.85
19_V	30_A	1.404	0.85
196_V	202_Q	1.401	0.85
159_L	163_Y	1.397	0.85
59_F	64_A	1.391	0.84
135_L	139_I	1.388	0.84
102_S	105_S	1.382	0.84
10_Y	300_L	1.373	0.84
186_N	203_S	1.358	0.83
195_T	198_N	1.314	0.81
299_E	302_R	1.308	0.80
287_D	290_V	1.308	0.80
9_A	256_Q	1.298	0.80
196_V	199_V	1.294	0.80
258_T	271_G	1.287	0.79
196_V	200_Q	1.277	0.79
15_V	31_I	1.276	0.79
261_V	267_L	1.272	0.79
123_F	127_L	1.268	0.78
92_E	102_S	1.267	0.78
167_M	172_K	1.255	0.78
123_F	128_V	1.254	0.77
10_Y	68_E	1.251	0.77
78_N	114_G	1.248	0.77
10_Y	297_F	1.237	0.77
151_S	222_M	1.226	0.76
54_V	57_I	1.216	0.75
154_K	225_Y	1.216	0.75
19_V	174_V	1.190	0.74
140_F	149_I	1.186	0.73
224_V	247_V	1.170	0.72
15_V	78_N	1.160	0.72
136_T	149_I	1.157	0.72
60_L	143_N	1.150	0.71
149_I	153_F	1.148	0.71
15_V	19_V	1.141	0.70
146_Q	218_I	1.124	0.69
269_L	272_Y	1.118	0.69
228_V	243_L	1.116	0.69
157_L	247_V	1.116	0.69
159_L	277_L	1.112	0.68
120_F	124_V	1.091	0.67
38_I	292_V	1.081	0.66
89_S	102_S	1.070	0.65
64_A	268_K	1.054	0.64
8_P	23_G	1.048	0.64
28_V	296_S	1.046	0.64
131_F	135_L	1.046	0.64
29_T	79_F	1.045	0.64
39_D	83_R	1.040	0.63
220_V	254_V	1.036	0.63
112_V	116_L	1.035	0.63
268_K	272_Y	1.034	0.63
184_V	297_F	1.033	0.63
113_I	116_L	1.033	0.63
172_K	176_Q	1.018	0.61
132_L	136_T	1.018	0.61
28_V	299_E	1.017	0.61
227_L	255_L	1.015	0.61
134_E	137_R	1.012	0.61
146_Q	230_T	1.007	0.61
165_L	175_F	1.001	0.60
30_A	40_F	0.994	0.60
188_Y	207_Y	0.989	0.59
195_T	294_I	0.988	0.59
212_S	276_W	0.985	0.59
59_F	249_G	0.981	0.59
296_S	301_L	0.978	0.58
158_L	217_T	0.974	0.58
147_I	226_L	0.973	0.58
185_I	204_R	0.972	0.58
226_L	247_V	0.968	0.58
65_A	215_L	0.964	0.57
29_T	76_H	0.959	0.57
242_A	295_E	0.959	0.57
33_R	83_R	0.943	0.56
140_F	145_A	0.938	0.55
51_L	55_K	0.935	0.55
33_R	37_S	0.930	0.54
166_S	175_F	0.927	0.54
23_G	301_L	0.922	0.54
48_N	52_N	0.920	0.54
140_F	153_F	0.920	0.54
218_I	251_S	0.914	0.53
57_I	223_F	0.910	0.53
212_S	271_G	0.900	0.52
145_A	295_E	0.899	0.52
259_N	271_G	0.898	0.52
127_L	131_F	0.897	0.52
102_S	108_L	0.895	0.52
65_A	69_A	0.892	0.51
16_V	216_F	0.876	0.50
17_E	173_R	0.876	0.50
157_L	160_G	0.874	0.50
158_L	162_I	0.870	0.50
220_V	224_V	0.869	0.50
298_N	302_R	0.869	0.50
154_K	239_D	0.866	0.49
19_V	277_L	0.865	0.49
144_T	147_I	0.864	0.49
142_L	145_A	0.854	0.48
196_V	288_E	0.854	0.48
221_G	225_Y	0.853	0.48
120_F	157_L	0.853	0.48
295_E	299_E	0.853	0.48
187_C	204_R	0.852	0.48
36_G	277_L	0.850	0.48
212_S	236_R	0.841	0.47
42_K	299_E	0.835	0.47
81_S	103_G	0.833	0.47
79_F	201_N	0.833	0.47
120_F	126_T	0.832	0.46
132_L	148_A	0.832	0.46
139_I	288_E	0.831	0.46
115_V	172_K	0.830	0.46
49_P	55_K	0.829	0.46
91_D	105_S	0.826	0.46
135_L	223_F	0.826	0.46
239_D	242_A	0.826	0.46
23_G	188_Y	0.824	0.46
81_S	170_L	0.823	0.46
39_D	79_F	0.823	0.46
74_T	78_N	0.820	0.46
147_I	215_L	0.815	0.45
34_T	247_V	0.812	0.45
70_S	105_S	0.800	0.44
205_L	289_Q	0.795	0.44
31_I	88_P	0.793	0.43
184_V	202_Q	0.792	0.43
92_E	103_G	0.791	0.43
217_T	221_G	0.788	0.43
237_V	280_L	0.786	0.43
105_S	108_L	0.786	0.43
60_L	153_F	0.785	0.43
49_P	56_K	0.785	0.43
22_G	256_Q	0.781	0.42
113_I	172_K	0.780	0.42
160_G	164_F	0.778	0.42
128_V	291_E	0.778	0.42
162_I	218_I	0.777	0.42
52_N	112_V	0.776	0.42
78_N	82_E	0.769	0.41
257_L	267_L	0.767	0.41
293_A	296_S	0.766	0.41
283_K	286_K	0.766	0.41
255_L	259_N	0.764	0.41
122_K	153_F	0.762	0.41
219_I	223_F	0.761	0.41
193_P	226_L	0.759	0.41
225_Y	243_L	0.755	0.40
199_V	287_D	0.754	0.40
160_G	234_W	0.753	0.40
115_V	295_E	0.753	0.40
29_T	43_L	0.748	0.40
248_L	300_L	0.745	0.40
117_S	294_I	0.742	0.39
114_G	118_F	0.740	0.39
136_T	204_R	0.739	0.39
82_E	106_M	0.737	0.39
160_G	163_Y	0.734	0.39
70_S	215_L	0.732	0.39
51_L	57_I	0.731	0.38
204_R	248_L	0.730	0.38
15_V	255_L	0.728	0.38
46_K	50_K	0.727	0.38
31_I	84_Y	0.726	0.38
32_R	38_I	0.726	0.38
146_Q	150_E	0.726	0.38
39_D	42_K	0.725	0.38
259_N	300_L	0.724	0.38
228_V	231_D	0.723	0.38
68_E	72_N	0.723	0.38
252_F	296_S	0.717	0.37
63_I	248_L	0.715	0.37
298_N	303_L	0.714	0.37
118_F	121_S	0.712	0.37
216_F	224_V	0.709	0.37
8_P	188_Y	0.708	0.37
133_A	146_Q	0.706	0.37
235_L	247_V	0.704	0.36
166_S	198_N	0.703	0.36
31_I	86_L	0.699	0.36
53_I	156_I	0.694	0.36
40_F	220_V	0.689	0.35
107_Y	110_L	0.688	0.35
91_D	108_L	0.685	0.35
43_L	241_V	0.685	0.35
122_K	127_L	0.682	0.35
26_H	302_R	0.681	0.35
8_P	24_K	0.678	0.34
25_H	90_E	0.677	0.34
188_Y	202_Q	0.676	0.34
148_A	152_L	0.675	0.34
66_I	271_G	0.674	0.34
54_V	280_L	0.673	0.34
150_E	240_R	0.672	0.34
63_I	246_V	0.669	0.34
64_A	148_A	0.666	0.34
197_E	287_D	0.666	0.34
272_Y	275_L	0.665	0.34
167_M	175_F	0.663	0.33
92_E	234_W	0.663	0.33
107_Y	111_A	0.662	0.33
130_V	230_T	0.660	0.33
34_T	136_T	0.658	0.33
154_K	245_P	0.655	0.33
207_Y	297_F	0.653	0.33
230_T	236_R	0.653	0.33
227_L	266_G	0.651	0.33
12_G	18_G	0.651	0.33
11_G	18_G	0.651	0.33
205_L	218_I	0.650	0.32
204_R	228_V	0.649	0.32
33_R	39_D	0.648	0.32
80_S	215_L	0.648	0.32
217_T	247_V	0.647	0.32
220_V	266_G	0.646	0.32
12_G	15_V	0.646	0.32
11_G	15_V	0.646	0.32
221_G	224_V	0.645	0.32
59_F	72_N	0.645	0.32
216_F	255_L	0.645	0.32
258_T	267_L	0.644	0.32
59_F	90_E	0.644	0.32
64_A	190_Q	0.644	0.32
186_N	300_L	0.643	0.32
187_C	263_D	0.643	0.32
74_T	248_L	0.643	0.32
184_V	188_Y	0.642	0.32
119_L	124_V	0.640	0.32
257_L	304_E	0.640	0.32
239_D	252_F	0.637	0.32
224_V	241_V	0.631	0.31
134_E	233_L	0.630	0.31
280_L	283_K	0.629	0.31
188_Y	306_L	0.628	0.31
60_L	265_P	0.627	0.31
23_G	229_P	0.626	0.31
7_P	268_K	0.626	0.31
103_G	135_L	0.624	0.31
23_G	150_E	0.624	0.31
136_T	140_F	0.623	0.31
33_R	84_Y	0.623	0.31
133_A	234_W	0.622	0.30
162_I	277_L	0.622	0.30
304_E	307_S	0.620	0.30
49_P	52_N	0.619	0.30
119_L	122_K	0.616	0.30
7_P	114_G	0.616	0.30
126_T	254_V	0.615	0.30
24_K	243_L	0.614	0.30
82_E	196_V	0.612	0.30
15_V	24_K	0.612	0.30
170_L	211_S	0.610	0.30
27_Y	45_R	0.609	0.30
109_S	112_V	0.608	0.30
86_L	159_L	0.608	0.30
67_I	139_I	0.608	0.30
35_D	280_L	0.606	0.29
135_L	242_A	0.605	0.29
14_A	18_G	0.603	0.29
184_V	301_L	0.602	0.29
130_V	150_E	0.601	0.29
69_A	274_G	0.598	0.29
29_T	299_E	0.598	0.29
38_I	248_L	0.597	0.29
224_V	243_L	0.596	0.29
134_E	162_I	0.595	0.29
33_R	184_V	0.593	0.28
86_L	152_L	0.592	0.28
82_E	85_G	0.591	0.28
76_H	79_F	0.589	0.28
23_G	302_R	0.586	0.28
71_A	261_V	0.586	0.28
239_D	275_L	0.585	0.28
39_D	85_G	0.584	0.28
116_L	145_A	0.584	0.28
22_G	151_S	0.583	0.28
44_P	136_T	0.583	0.28
10_Y	204_R	0.582	0.28
121_S	240_R	0.581	0.28
192_L	196_V	0.581	0.28
222_M	241_V	0.579	0.28
34_T	39_D	0.579	0.28
188_Y	301_L	0.579	0.28
84_Y	106_M	0.578	0.28
138_P	141_S	0.576	0.27
53_I	57_I	0.576	0.27
166_S	301_L	0.575	0.27
44_P	184_V	0.575	0.27
47_H	50_K	0.571	0.27
161_Y	185_I	0.571	0.27
153_F	170_L	0.571	0.27
51_L	169_P	0.570	0.27
187_C	264_I	0.565	0.27
47_H	293_A	0.563	0.27
127_L	241_V	0.563	0.27
31_I	83_R	0.561	0.26
298_N	306_L	0.561	0.26
212_S	258_T	0.557	0.26
42_K	79_F	0.557	0.26
167_M	257_L	0.557	0.26
174_V	215_L	0.556	0.26
28_V	300_L	0.555	0.26
214_I	217_T	0.555	0.26
24_K	224_V	0.554	0.26
186_N	190_Q	0.554	0.26
297_F	304_E	0.552	0.26
155_L	174_V	0.551	0.26
60_L	279_L	0.551	0.26
9_A	23_G	0.550	0.26
23_G	184_V	0.550	0.26
197_E	201_N	0.545	0.25
195_T	232_P	0.544	0.25
71_A	82_E	0.542	0.25
223_F	299_E	0.541	0.25
31_I	246_V	0.541	0.25
200_Q	284_E	0.539	0.25
118_F	214_I	0.538	0.25
217_T	251_S	0.538	0.25
26_H	54_V	0.538	0.25
225_Y	242_A	0.538	0.25
48_N	51_L	0.536	0.25
35_D	90_E	0.536	0.25
154_K	221_G	0.534	0.25
174_V	214_I	0.534	0.25
59_F	128_V	0.533	0.25
266_G	269_L	0.532	0.25
187_C	202_Q	0.531	0.25
90_E	102_S	0.530	0.24
205_L	275_L	0.529	0.24
29_T	85_G	0.526	0.24
233_L	241_V	0.525	0.24
132_L	224_V	0.524	0.24
17_E	20_M	0.524	0.24
115_V	167_M	0.524	0.24
167_M	192_L	0.521	0.24
227_L	254_V	0.520	0.24
276_W	279_L	0.520	0.24
160_G	172_K	0.520	0.24
186_N	204_R	0.519	0.24
43_L	120_F	0.519	0.24
245_P	249_G	0.519	0.24
38_I	294_I	0.518	0.24
28_V	40_F	0.517	0.24
38_I	176_Q	0.516	0.24
220_V	247_V	0.516	0.24
231_D	296_S	0.515	0.24
77_L	80_S	0.515	0.24
23_G	146_Q	0.514	0.24
111_A	241_V	0.511	0.23
106_M	109_S	0.510	0.23
66_I	187_C	0.510	0.23
148_A	287_D	0.509	0.23
73_G	273_P	0.509	0.23
19_V	153_F	0.506	0.23
220_V	251_S	0.505	0.23
46_K	67_I	0.503	0.23
79_F	280_L	0.503	0.23
204_R	229_P	0.503	0.23
116_L	135_L	0.502	0.23
150_E	229_P	0.501	0.23
163_Y	175_F	0.501	0.23
130_V	233_L	0.500	0.23
265_P	275_L	0.500	0.23

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4q9hA	1	0.6164	7	0.982	Contact Map
4x01A	4	0.1447	6.6	0.983	Contact Map
1y9lA	1	0.1447	4.9	0.984	Contact Map
3zdqA	2	0.6415	4.6	0.984	Contact Map
3wmgA	1	0.6352	4.4	0.984	Contact Map
4mnoA	1	0.283	4.3	0.984	Contact Map
4aybP	1	0.1352	4.1	0.984	Contact Map
3e5dA	2	0.1352	4	0.984	Contact Map
4f4cA	1	0.7704	3.5	0.985	Contact Map
4q4hA	1	0.6289	3.4	0.985	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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