YQGM - BACSU - GREMLIN Contacts

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YQGM - Uncharacterized glycosyltransferase YqgM

UniProt:	P54490	Sequences: MNILMLSPEHPDEPKSGLGVHLNRLISYLNPHINITVFTPSGQLFSYAKFEDYIADANFTMVRHVLSHNKRFDLIHAHDDTTAPAAQYLKQRLGLPLAATIHGLESERKKVCREAPHPYRLMTERLLIESADALIVLSTFMKRSLDKAAHKKITVIPSPASMEEEKGKIPRSMNRRFLFSYGRFVPEKGLSQLLKVFAILKQRQPDLYLVLAGEGPSLSCYEKLAAAYNLKDRVMFLPFLNRKDIRTLLSHCEMAVFPSSYEPFGLAAQESMEQGVLTVVSQSGGFCDYAVHDKTAIIADFTLVQEAADLLDSFLKDREKARRIKEAGRQQVFKLHHPRLIMSSYLQLYERIFNNSAIH	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	359 (331)
Sequences:	58098 (49279)
Seq/√Len:	2708.6

ECOLI - WCAL RFAG GLGA RFAB WBBK WCAI WCAC
BACSU - YQGM YPJH YTCC COTSA GLGA EPSF EPSD TUAC YKVP TUAH CGEB TAGE
HALSA - Q9HSW1 Q9HQQ0 Q9HS45 Q9HRP8 Q9HQT2 Q9HSV4 Q9HSV2 Q9HRP9
SULSO - Q980C8 Q97XT7 Q97V61 Q97UM0 Q97V80 Q97ZD3 Q97VL4 Q97XT8 Q97XU2 Q97UU3 Q7LXI2 Q97XT5 Q97XT9 Q97XT6 Q7LXM9 Q97XT1 Q97XF6 Q7LXF8 Q7LXF5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
179_F	252_C	4.605	1.00
208_Y	235_M	3.654	1.00
218_L	222_E	3.301	1.00
133_A	154_T	3.208	1.00
255_A	271_S	3.052	1.00
177_F	210_V	2.899	1.00
76_H	349_Y	2.859	1.00
199_I	308_A	2.700	1.00
210_V	237_L	2.696	1.00
195_K	224_L	2.685	1.00
331_Q	335_L	2.682	1.00
175_R	208_Y	2.628	1.00
180_S	190_L	2.469	1.00
201_K	233_R	2.381	1.00
142_K	146_D	2.266	1.00
36_T	72_F	2.178	1.00
195_K	304_V	2.152	1.00
189_G	192_Q	2.129	1.00
176_R	315_L	2.125	1.00
217_S	220_C	2.100	1.00
177_F	208_Y	2.039	1.00
329_R	333_F	2.017	1.00
282_Q	287_C	2.017	1.00
224_L	228_Y	2.006	1.00
212_A	248_L	2.006	1.00
199_I	305_Q	2.000	1.00
317_D	320_K	1.969	1.00
310_L	313_S	1.957	1.00
197_F	209_L	1.955	1.00
177_F	251_H	1.943	1.00
36_T	70_K	1.936	1.00
210_V	235_M	1.933	1.00
33_I	346_L	1.900	1.00
176_R	253_E	1.888	1.00
290_A	296_A	1.884	1.00
295_T	331_Q	1.870	1.00
178_L	254_M	1.864	1.00
313_S	320_K	1.846	1.00
291_V	294_K	1.834	1.00
321_A	325_K	1.799	1.00
275_G	329_R	1.778	1.00
340_L	344_S	1.757	1.00
226_A	231_K	1.740	1.00
76_H	100_T	1.734	1.00
282_Q	300_D	1.733	1.00
139_T	142_K	1.732	1.00
189_G	260_S	1.723	1.00
300_D	306_E	1.719	1.00
154_T	351_R	1.719	1.00
297_I	310_L	1.714	1.00
159_P	338_P	1.682	1.00
133_A	348_L	1.672	1.00
313_S	317_D	1.663	1.00
193_L	209_L	1.660	1.00
142_K	155_V	1.654	1.00
255_A	276_V	1.649	1.00
139_T	288_D	1.648	1.00
99_A	131_A	1.615	1.00
136_V	153_I	1.610	1.00
198_A	228_Y	1.603	1.00
312_D	316_K	1.571	1.00
294_K	327_A	1.568	1.00
181_Y	245_I	1.562	1.00
197_F	207_L	1.562	1.00
320_K	323_R	1.542	1.00
193_L	256_V	1.533	1.00
31_P	343_S	1.528	1.00
291_V	331_Q	1.528	1.00
346_L	350_E	1.512	1.00
303_L	306_E	1.504	1.00
214_E	218_L	1.501	1.00
200_L	312_D	1.489	1.00
241_N	244_D	1.478	1.00
291_V	295_T	1.469	1.00
306_E	310_L	1.467	1.00
297_I	313_S	1.462	1.00
142_K	153_I	1.461	1.00
323_R	327_A	1.461	1.00
139_T	155_V	1.441	1.00
192_Q	260_S	1.426	1.00
28_Y	339_R	1.415	1.00
28_Y	342_M	1.415	1.00
23_N	27_S	1.413	1.00
177_F	253_E	1.403	1.00
212_A	237_L	1.400	1.00
187_E	261_Y	1.381	1.00
240_L	244_D	1.380	1.00
192_Q	304_V	1.367	1.00
210_V	248_L	1.361	1.00
280_V	286_F	1.360	1.00
252_C	276_V	1.355	1.00
244_D	247_T	1.341	1.00
146_D	150_H	1.338	1.00
191_S	224_L	1.324	1.00
135_I	345_Y	1.298	1.00
149_A	152_K	1.296	1.00
135_I	348_L	1.295	1.00
304_V	308_A	1.289	1.00
190_L	211_L	1.283	1.00
196_V	307_A	1.282	1.00
210_V	252_C	1.282	1.00
178_L	256_V	1.281	1.00
299_A	310_L	1.278	1.00
180_S	193_L	1.273	1.00
246_R	274_Q	1.266	1.00
172_S	175_R	1.265	1.00
184_F	211_L	1.263	1.00
199_I	304_V	1.252	1.00
178_L	311_L	1.249	1.00
139_T	143_R	1.247	1.00
31_P	346_L	1.244	1.00
344_S	347_Q	1.242	1.00
327_A	330_Q	1.228	1.00
340_L	343_S	1.221	1.00
77_A	97_L	1.217	1.00
223_K	227_A	1.211	1.00
136_V	142_K	1.204	1.00
179_F	212_A	1.202	1.00
165_E	274_Q	1.198	1.00
5_M	29_L	1.196	1.00
5_M	78_H	1.190	1.00
306_E	309_D	1.190	1.00
176_R	254_M	1.159	1.00
133_A	351_R	1.157	1.00
143_R	146_D	1.153	1.00
224_L	227_A	1.146	1.00
260_S	302_T	1.132	1.00
245_I	249_L	1.127	1.00
264_F	269_Q	1.126	1.00
250_S	274_Q	1.125	1.00
242_R	245_I	1.124	1.00
322_R	326_E	1.122	1.00
219_S	222_E	1.115	1.00
325_K	328_G	1.103	1.00
259_S	262_E	1.098	1.00
308_A	312_D	1.092	1.00
198_A	202_Q	1.092	1.00
28_Y	31_P	1.092	1.00
318_R	321_A	1.086	1.00
136_V	145_L	1.084	1.00
219_S	223_K	1.083	1.00
191_S	195_K	1.076	1.00
268_A	286_F	1.075	1.00
164_E	246_R	1.075	1.00
222_E	226_A	1.074	1.00
280_V	298_I	1.071	1.00
176_R	207_L	1.070	1.00
292_H	310_L	1.068	1.00
299_A	307_A	1.064	1.00
337_H	340_L	1.061	1.00
281_S	299_A	1.061	1.00
257_F	268_A	1.054	1.00
212_A	240_L	1.053	1.00
284_G	287_C	1.048	1.00
177_F	252_C	1.040	1.00
330_Q	333_F	1.038	1.00
276_V	325_K	1.037	1.00
21_H	266_L	1.032	1.00
192_Q	281_S	1.029	1.00
102_H	266_L	1.026	1.00
341_I	344_S	1.026	1.00
249_L	274_Q	1.025	1.00
192_Q	195_K	1.023	1.00
275_G	326_E	1.022	1.00
338_P	341_I	1.019	1.00
192_Q	302_T	1.018	1.00
257_F	267_A	1.012	1.00
272_M	278_T	1.011	1.00
137_L	158_S	1.010	1.00
210_V	251_H	1.009	1.00
320_K	324_I	1.009	1.00
176_R	206_D	1.005	1.00
255_A	267_A	1.002	1.00
258_P	307_A	1.002	1.00
137_L	141_M	0.999	1.00
183_R	187_E	0.991	1.00
225_A	236_F	0.990	1.00
191_S	260_S	0.989	1.00
175_R	206_D	0.986	1.00
265_G	286_F	0.986	1.00
343_S	347_Q	0.985	1.00
199_I	202_Q	0.983	1.00
326_E	330_Q	0.982	1.00
237_L	248_L	0.978	1.00
143_R	147_K	0.975	1.00
200_L	207_L	0.974	1.00
181_Y	212_A	0.974	1.00
297_I	324_I	0.973	1.00
309_D	313_S	0.972	1.00
285_G	288_D	0.966	1.00
5_M	26_I	0.965	1.00
179_F	248_L	0.965	1.00
191_S	220_C	0.965	1.00
246_R	250_S	0.964	1.00
89_L	93_L	0.963	1.00
181_Y	249_L	0.963	1.00
262_E	265_G	0.955	1.00
178_L	197_F	0.954	1.00
24_R	27_S	0.951	1.00
341_I	345_Y	0.949	1.00
136_V	141_M	0.944	1.00
65_V	69_N	0.941	1.00
67_S	72_F	0.936	1.00
314_F	324_I	0.933	1.00
66_L	70_K	0.931	1.00
297_I	314_F	0.931	1.00
16_S	19_G	0.929	1.00
78_H	102_H	0.926	1.00
240_L	248_L	0.924	1.00
237_L	240_L	0.924	1.00
165_E	246_R	0.923	1.00
194_L	225_A	0.920	1.00
195_K	228_Y	0.919	1.00
97_L	131_A	0.916	1.00
200_L	311_L	0.915	1.00
158_S	266_L	0.913	1.00
318_R	322_R	0.909	1.00
144_S	147_K	0.908	1.00
272_M	328_G	0.906	1.00
26_I	37_V	0.906	1.00
131_A	134_L	0.904	1.00
77_A	99_A	0.903	1.00
193_L	258_P	0.902	1.00
154_T	348_L	0.901	1.00
162_M	337_H	0.900	1.00
279_V	299_A	0.893	1.00
188_K	262_E	0.891	1.00
325_K	329_R	0.889	1.00
137_L	263_P	0.886	1.00
180_S	211_L	0.884	1.00
295_T	328_G	0.882	1.00
197_F	233_R	0.881	1.00
200_L	315_L	0.881	1.00
268_A	280_V	0.880	1.00
271_S	276_V	0.878	1.00
225_A	230_L	0.877	1.00
159_P	341_I	0.874	1.00
207_L	315_L	0.873	1.00
192_Q	307_A	0.873	1.00
322_R	325_K	0.867	1.00
24_R	338_P	0.863	1.00
280_V	290_A	0.863	1.00
277_L	325_K	0.861	1.00
324_I	327_A	0.860	1.00
300_D	303_L	0.858	1.00
208_Y	233_R	0.858	1.00
154_T	344_S	0.853	1.00
272_M	331_Q	0.850	1.00
297_I	320_K	0.847	1.00
98_A	135_I	0.846	1.00
21_H	102_H	0.846	1.00
198_A	201_K	0.841	1.00
290_A	298_I	0.838	1.00
242_R	246_R	0.835	1.00
268_A	278_T	0.834	1.00
196_V	308_A	0.830	1.00
185_V	216_P	0.828	1.00
161_S	164_E	0.827	1.00
200_L	308_A	0.827	1.00
179_F	249_L	0.823	1.00
191_S	217_S	0.819	1.00
292_H	295_T	0.813	1.00
180_S	258_P	0.812	1.00
254_M	314_F	0.812	1.00
337_H	341_I	0.809	1.00
135_I	156_I	0.808	1.00
272_M	295_T	0.807	1.00
305_Q	309_D	0.807	1.00
249_L	276_V	0.805	1.00
279_V	310_L	0.803	1.00
220_C	223_K	0.800	1.00
156_I	348_L	0.799	1.00
223_K	226_A	0.795	1.00
90_K	97_L	0.794	1.00
247_T	251_H	0.793	1.00
149_A	153_I	0.793	1.00
146_D	153_I	0.792	1.00
173_M	176_R	0.791	1.00
200_L	205_P	0.791	1.00
283_S	286_F	0.789	1.00
248_L	251_H	0.783	1.00
188_K	267_A	0.778	1.00
195_K	198_A	0.775	1.00
268_A	289_Y	0.775	1.00
256_V	311_L	0.774	1.00
257_F	286_F	0.773	1.00
196_V	304_V	0.772	1.00
20_V	24_R	0.771	1.00
343_S	346_L	0.771	1.00
140_F	143_R	0.769	1.00
220_C	224_L	0.769	1.00
148_A	152_K	0.767	1.00
331_Q	336_H	0.765	1.00
254_M	311_L	0.762	1.00
195_K	199_I	0.762	1.00
161_S	165_E	0.761	1.00
172_S	208_Y	0.758	1.00
122_M	125_R	0.758	1.00
22_L	78_H	0.756	1.00
201_K	205_P	0.755	1.00
188_K	259_S	0.753	1.00
277_L	314_F	0.753	1.00
347_Q	351_R	0.751	1.00
122_M	126_L	0.747	1.00
24_R	28_Y	0.745	1.00
157_P	344_S	0.744	1.00
180_S	255_A	0.736	1.00
186_P	191_S	0.735	1.00
88_Y	92_R	0.732	1.00
157_P	288_D	0.731	1.00
24_R	161_S	0.731	1.00
96_P	132_D	0.725	1.00
198_A	230_L	0.723	1.00
31_P	339_R	0.722	1.00
184_F	218_L	0.721	1.00
126_L	130_S	0.720	1.00
282_Q	298_I	0.719	1.00
225_A	234_V	0.716	1.00
98_A	133_A	0.715	1.00
36_T	40_P	0.714	1.00
347_Q	350_E	0.711	1.00
279_V	314_F	0.711	1.00
338_P	342_M	0.708	1.00
247_T	250_S	0.708	1.00
339_R	343_S	0.706	1.00
102_H	137_L	0.705	1.00
253_E	321_A	0.701	1.00
140_F	144_S	0.700	1.00
309_D	312_D	0.699	1.00
76_H	345_Y	0.696	1.00
21_H	100_T	0.692	1.00
281_S	301_F	0.687	1.00
156_I	345_Y	0.685	1.00
345_Y	349_Y	0.683	1.00
16_S	183_R	0.683	1.00
74_L	349_Y	0.681	1.00
287_C	298_I	0.681	1.00
323_R	326_E	0.679	1.00
180_S	209_L	0.678	1.00
186_P	217_S	0.678	1.00
7_S	18_L	0.677	1.00
181_Y	255_A	0.672	1.00
148_A	151_K	0.671	1.00
157_P	336_H	0.669	1.00
20_V	23_N	0.668	1.00
281_S	307_A	0.668	1.00
21_H	158_S	0.668	1.00
186_P	260_S	0.664	1.00
225_A	228_Y	0.663	1.00
264_F	336_H	0.663	1.00
305_Q	308_A	0.661	1.00
324_I	328_G	0.660	1.00
137_L	266_L	0.660	1.00
182_G	190_L	0.660	1.00
77_A	83_A	0.659	1.00
178_L	207_L	0.659	1.00
265_G	268_A	0.657	1.00
213_G	238_P	0.657	1.00
293_D	313_S	0.654	1.00
90_K	93_L	0.651	1.00
168_K	250_S	0.647	1.00
269_Q	336_H	0.647	1.00
91_Q	130_S	0.647	1.00
292_H	297_I	0.646	1.00
313_S	316_K	0.646	1.00
295_T	327_A	0.645	1.00
31_P	342_M	0.645	1.00
74_L	98_A	0.644	1.00
162_M	333_F	0.644	1.00
272_M	325_K	0.644	1.00
103_G	187_E	0.642	1.00
78_H	100_T	0.640	1.00
182_G	188_K	0.639	1.00
206_D	233_R	0.638	1.00
201_K	207_L	0.636	1.00
118_P	121_L	0.635	1.00
154_T	157_P	0.632	1.00
32_H	346_L	0.631	1.00
319_E	322_R	0.629	1.00
197_F	200_L	0.626	1.00
329_R	332_V	0.625	1.00
222_E	236_F	0.623	1.00
319_E	323_R	0.621	1.00
176_R	318_R	0.616	1.00
178_L	315_L	0.615	1.00
25_L	345_Y	0.615	1.00
21_H	345_Y	0.614	1.00
98_A	349_Y	0.612	1.00
314_F	321_A	0.612	1.00
156_I	344_S	0.612	1.00
66_L	69_N	0.612	1.00
162_M	273_E	0.611	1.00
196_V	311_L	0.611	1.00
7_S	22_L	0.608	1.00
290_A	295_T	0.608	1.00
256_V	307_A	0.604	1.00
125_R	129_E	0.604	1.00
190_L	217_S	0.603	1.00
90_K	132_D	0.602	1.00
257_F	265_G	0.602	1.00
65_V	68_H	0.600	1.00
59_F	63_R	0.597	1.00
328_G	331_Q	0.596	1.00
299_A	306_E	0.595	1.00
140_F	287_C	0.594	1.00
75_I	90_K	0.592	1.00
248_L	252_C	0.592	1.00
342_M	346_L	0.590	1.00
348_L	351_R	0.589	1.00
79_D	82_T	0.585	1.00
193_L	197_F	0.585	1.00
176_R	179_F	0.584	1.00
26_I	35_I	0.582	1.00
205_P	316_K	0.581	1.00
5_M	35_I	0.581	1.00
77_A	86_A	0.581	1.00
179_F	255_A	0.581	1.00
194_L	234_V	0.576	1.00
67_S	70_K	0.576	1.00
72_F	95_L	0.576	1.00
326_E	329_R	0.574	1.00
255_A	278_T	0.574	1.00
9_E	19_G	0.573	1.00
177_F	248_L	0.573	1.00
166_K	250_S	0.571	1.00
317_D	321_A	0.570	1.00
27_S	30_N	0.568	1.00
217_S	221_Y	0.567	1.00
254_M	279_V	0.565	1.00
136_V	155_V	0.565	1.00
189_G	258_P	0.563	1.00
54_I	57_A	0.561	1.00
100_T	345_Y	0.560	1.00
33_I	350_E	0.560	1.00
225_A	231_K	0.559	1.00
212_A	245_I	0.558	1.00
150_H	153_I	0.557	1.00
97_L	132_D	0.553	1.00
100_T	135_I	0.553	1.00
192_Q	196_V	0.553	1.00
191_S	221_Y	0.552	1.00
179_F	276_V	0.550	1.00
32_H	35_I	0.548	1.00
327_A	331_Q	0.547	1.00
196_V	200_L	0.546	1.00
141_M	144_S	0.546	1.00
189_G	302_T	0.543	1.00
30_N	339_R	0.543	1.00
215_G	218_L	0.543	1.00
176_R	321_A	0.542	1.00
69_N	72_F	0.542	1.00
240_L	245_I	0.538	1.00
138_S	141_M	0.537	1.00
179_F	210_V	0.536	1.00
194_L	221_Y	0.536	1.00
5_M	76_H	0.536	1.00
253_E	318_R	0.536	1.00
184_F	190_L	0.536	1.00
271_S	278_T	0.535	1.00
20_V	242_R	0.535	1.00
189_G	281_S	0.534	1.00
174_N	206_D	0.534	1.00
209_L	234_V	0.534	1.00
214_E	239_F	0.532	1.00
283_S	287_C	0.530	1.00
283_S	301_F	0.529	1.00
146_D	149_A	0.529	1.00
10_H	23_N	0.528	1.00
142_K	150_H	0.528	1.00
186_P	220_C	0.525	1.00
249_L	267_A	0.525	1.00
157_P	341_I	0.525	1.00
75_I	95_L	0.525	1.00
139_T	157_P	0.525	1.00
321_A	324_I	0.522	1.00
265_G	269_Q	0.520	1.00
18_L	79_D	0.520	1.00
221_Y	236_F	0.520	1.00
24_R	266_L	0.520	1.00
258_P	281_S	0.517	1.00
141_M	263_P	0.517	1.00
196_V	256_V	0.515	1.00
277_L	321_A	0.513	1.00
157_P	264_F	0.512	1.00
267_A	271_S	0.511	1.00
166_K	169_I	0.510	1.00
102_H	158_S	0.509	1.00
105_E	108_R	0.509	1.00
335_L	340_L	0.508	1.00
272_M	332_V	0.508	1.00
38_F	66_L	0.506	1.00
269_Q	332_V	0.505	1.00
60_T	63_R	0.504	1.00
21_H	78_H	0.503	1.00
101_I	141_M	0.503	1.00
282_Q	301_F	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2r60A	1	0.9833	100	0.151	Contact Map
4n9wA	1	0.9276	100	0.154	Contact Map
3c48A	2	0.9638	100	0.166	Contact Map
3froA	3	0.9833	100	0.182	Contact Map
1rzuA	1	0.9694	100	0.186	Contact Map
2x6qA	1	0.9276	100	0.193	Contact Map
3okpA	1	0.9526	100	0.195	Contact Map
2qzsA	1	0.961	100	0.201	Contact Map
3oy2A	2	0.9415	100	0.204	Contact Map
2jjmA	4	0.9164	100	0.208	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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