GREMLIN Database
ISPG - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
UniProt: P54482 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 377 (348)
Sequences: 1750 (760)
Seq/√Len: 40.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
193_E230_K4.9031.00
76_K103_D3.8811.00
144_R163_H3.5441.00
14_P26_N3.3201.00
279_D282_S3.0861.00
58_I84_V3.0581.00
347_E350_K3.0231.00
155_D194_K3.0131.00
14_P21_T2.6541.00
16_K21_T2.6261.00
96_K99_E2.5901.00
115_R170_D2.5891.00
15_V177_I2.5571.00
189_I226_A2.5101.00
166_K170_D2.4251.00
165_I178_V2.3981.00
188_A207_I2.3911.00
45_V79_I2.3541.00
217_T250_V2.3451.00
45_V78_R2.3161.00
13_R174_H2.3141.00
52_A80_S2.3111.00
326_E338_K2.2901.00
74_D77_K2.2791.00
216_G240_L2.2541.00
50_R243_D2.2241.00
10_T196_A2.2231.00
266_T299_K2.2141.00
104_K177_I2.2101.00
36_T44_T2.1981.00
327_G344_M2.1870.99
41_V45_V2.1780.99
238_I254_L2.1620.99
98_I125_A2.1610.99
79_I83_L2.1410.99
98_I130_I2.1340.99
12_T196_A2.0530.99
142_E160_S2.0120.99
49_N80_S2.0110.99
291_I300_V1.9830.99
283_I345_V1.9750.99
40_D43_A1.9630.99
142_E163_H1.9340.99
340_P343_T1.9210.99
39_H65_D1.8980.99
120_A123_K1.8830.98
129_G175_D1.8820.98
32_S51_L1.8810.98
178_V195_A1.8530.98
290_Y349_K1.8390.98
178_V199_F1.8140.98
222_A254_L1.7790.98
84_V104_K1.7780.98
73_A100_G1.7650.98
30_I252_R1.7520.97
52_A79_I1.7450.97
14_P25_N1.7390.97
148_K159_E1.7280.97
241_S269_S1.7270.97
180_M195_A1.7270.97
13_R200_D1.7120.97
16_K19_P1.6910.97
278_I322_G1.6840.97
169_E201_Y1.6780.97
30_I251_A1.6710.97
42_E78_R1.6700.97
46_A50_R1.6530.96
242_A246_E1.6500.96
34_T44_T1.6240.96
277_E324_R1.6240.96
45_V80_S1.6130.96
337_R347_E1.5970.96
57_Q261_A1.5940.96
122_V171_L1.5790.95
318_I355_L1.5740.95
167_I171_L1.5660.95
50_R245_V1.5610.95
351_E354_I1.5580.95
122_V168_L1.5290.94
145_I156_G1.5290.94
282_S286_E1.5140.94
343_T347_E1.5120.94
246_E249_K1.5100.94
325_G341_E1.4920.94
61_V79_I1.4700.93
29_V58_I1.4700.93
38_T44_T1.4570.93
72_I102_A1.4390.92
221_A225_G1.4380.92
212_T284_A1.4280.92
76_K83_L1.4190.92
162_L199_F1.4190.92
138_A182_A1.4140.92
29_V57_Q1.4130.92
205_L236_M1.4100.92
29_V251_A1.4080.92
145_I163_H1.4070.91
119_E171_L1.4030.91
252_R263_N1.3960.91
348_L352_I1.3820.91
207_I223_G1.3690.90
226_A230_K1.3690.90
290_Y346_E1.3650.90
20_L29_V1.3610.90
119_E123_K1.3590.90
66_E96_K1.3580.90
224_L238_I1.3550.90
320_I344_M1.3520.90
195_A199_F1.3490.90
26_N29_V1.3460.90
138_A141_L1.3400.89
116_E120_A1.3360.89
350_K354_I1.3300.89
54_A245_V1.3150.88
329_L339_V1.3040.88
153_T156_G1.2970.88
349_K353_D1.2950.88
327_G339_V1.2870.87
118_V164_H1.2730.87
214_F253_E1.2680.87
329_L348_L1.2660.86
194_K198_A1.2630.86
49_N52_A1.2590.86
21_T25_N1.2550.86
182_A187_L1.2380.85
99_E124_A1.2340.85
105_I125_A1.2320.85
62_A274_G1.2280.85
14_P24_G1.2260.85
70_N96_K1.2220.84
226_A229_S1.2200.84
95_L102_A1.1800.82
115_R119_E1.1750.82
343_T346_E1.1740.82
286_E290_Y1.1690.82
21_T26_N1.1640.81
49_N78_R1.1560.81
35_T276_I1.1540.81
222_A225_G1.1530.81
157_M180_M1.1490.81
289_E292_S1.1470.80
249_K292_S1.1350.80
84_V177_I1.1310.79
11_K200_D1.1300.79
41_V75_I1.1260.79
15_V21_T1.1260.79
134_I164_H1.1210.79
155_D198_A1.1190.79
328_L338_K1.1120.78
337_R351_E1.1070.78
34_T38_T1.1020.78
98_I124_A1.0940.77
285_N289_E1.0940.77
242_A281_I1.0930.77
146_L314_R1.0820.76
39_H68_A1.0820.76
190_E193_E1.0800.76
331_R336_V1.0770.76
168_L173_F1.0770.76
95_L128_K1.0690.75
222_A226_A1.0690.75
41_V74_D1.0670.75
176_I201_Y1.0640.75
301_A316_A1.0590.75
319_G330_F1.0570.75
213_L242_A1.0500.74
126_K172_D1.0400.73
205_L227_I1.0400.73
44_T47_E1.0340.73
321_A330_F1.0280.73
212_T288_E1.0240.72
97_A100_G1.0140.72
21_T29_V1.0120.71
52_A81_I1.0100.71
117_K120_A1.0020.71
286_E289_E0.9900.70
95_L124_A0.9890.70
124_A127_D0.9860.69
69_A100_G0.9830.69
124_A128_K0.9730.68
318_I351_E0.9700.68
286_E345_V0.9670.68
328_L344_M0.9620.67
45_V76_K0.9570.67
164_H168_L0.9530.67
252_R261_A0.9530.67
17_V29_V0.9500.66
267_L291_I0.9460.66
222_A257_S0.9400.66
41_V71_A0.9400.66
25_N318_I0.9370.65
96_K100_G0.9360.65
155_D190_E0.9350.65
56_C81_I0.9350.65
107_I113_G0.9330.65
11_K25_N0.9280.65
165_I180_M0.9210.64
45_V49_N0.9200.64
42_E46_A0.9160.64
64_P68_A0.9040.63
221_A254_L0.9020.62
157_M187_L0.9010.62
65_D68_A0.9000.62
22_I29_V0.8990.62
146_L156_G0.8920.62
72_I76_K0.8920.62
13_R175_D0.8850.61
351_E355_L0.8830.61
157_M182_A0.8820.61
168_L176_I0.8820.61
76_K79_I0.8780.60
166_K169_E0.8690.59
331_R351_E0.8680.59
159_E163_H0.8680.59
236_M251_A0.8670.59
169_E174_H0.8670.59
27_E261_A0.8660.59
337_R350_K0.8650.59
287_V302_V0.8650.59
219_K266_T0.8620.59
291_I294_I0.8590.59
339_V347_E0.8580.58
306_A328_L0.8580.58
216_G242_A0.8560.58
35_T47_E0.8550.58
214_F217_T0.8540.58
188_A224_L0.8530.58
214_F249_K0.8530.58
10_T200_D0.8520.58
184_D266_T0.8490.58
326_E340_P0.8430.57
115_R167_I0.8400.57
309_G319_G0.8390.57
38_T71_A0.8370.57
44_T61_V0.8370.57
283_I286_E0.8370.57
35_T244_P0.8350.56
165_I199_F0.8340.56
49_N79_I0.8320.56
123_K127_D0.8290.56
162_L198_A0.8270.56
145_I148_K0.8250.56
229_S258_F0.8210.55
189_I229_S0.8200.55
34_T59_V0.8190.55
90_D93_L0.8150.55
62_A93_L0.8130.54
284_A302_V0.8120.54
20_L58_I0.8120.54
157_M165_I0.8110.54
10_T25_N0.8110.54
57_Q252_R0.8100.54
38_T47_E0.8080.54
45_V52_A0.8020.53
163_H166_K0.8020.53
184_D187_L0.7950.53
36_T47_E0.7940.53
241_S280_L0.7910.52
17_V104_K0.7910.52
207_I257_S0.7900.52
28_V236_M0.7870.52
66_E70_N0.7860.52
272_T276_I0.7860.52
189_I207_I0.7840.52
72_I97_A0.7830.52
207_I227_I0.7790.51
25_N234_N0.7760.51
158_V194_K0.7750.51
194_K197_K0.7750.51
214_F218_V0.7740.51
284_A287_V0.7740.51
242_A285_N0.7740.51
112_I118_V0.7730.51
99_E128_K0.7720.51
301_A310_P0.7690.50
37_K68_A0.7690.50
34_T48_I0.7650.50
59_V79_I0.7650.50
213_L246_E0.7640.50
149_Y153_T0.7630.50
180_M191_A0.7620.50
164_H167_I0.7580.49
83_L103_D0.7560.49
146_L152_P0.7550.49
17_V20_L0.7530.49
303_L316_A0.7530.49
278_I283_I0.7500.49
79_I103_D0.7500.49
220_S238_I0.7470.48
303_L310_P0.7450.48
287_V300_V0.7410.48
207_I226_A0.7400.48
318_I352_I0.7360.47
22_I177_I0.7360.47
142_E164_H0.7340.47
166_K201_Y0.7340.47
68_A274_G0.7330.47
16_K20_L0.7330.47
141_L154_A0.7320.47
267_L284_A0.7220.46
193_E226_A0.7180.46
162_L178_V0.7180.46
320_I328_L0.7160.45
212_T302_V0.7160.45
88_H274_G0.7150.45
80_S83_L0.7080.45
267_L302_V0.7080.45
313_A321_A0.7060.45
193_E229_S0.7050.44
294_I297_P0.7050.44
154_A187_L0.7040.44
57_Q79_I0.7030.44
154_A194_K0.7000.44
15_V104_K0.6990.44
349_K352_I0.6990.44
95_L99_E0.6960.44
71_A100_G0.6950.44
115_R171_L0.6940.43
39_H277_E0.6930.43
265_A291_I0.6930.43
49_N53_E0.6910.43
34_T47_E0.6900.43
22_I104_K0.6890.43
105_I199_F0.6880.43
37_K277_E0.6880.43
45_V103_D0.6810.42
213_L218_V0.6790.42
217_T221_A0.6780.42
287_V345_V0.6770.42
293_K353_D0.6760.42
215_A253_E0.6750.42
48_I71_A0.6730.42
121_V124_A0.6710.41
276_I303_L0.6700.41
75_I81_I0.6690.41
341_E348_L0.6690.41
91_Y95_L0.6680.41
56_C248_V0.6670.41
195_A263_N0.6660.41
38_T75_I0.6650.41
280_L341_E0.6610.40
143_K332_K0.6610.40
14_P29_V0.6610.40
331_R347_E0.6570.40
142_E183_S0.6570.40
258_F261_A0.6570.40
63_C69_A0.6490.39
57_Q263_N0.6480.39
221_A224_L0.6480.39
253_E256_K0.6460.39
346_E349_K0.6450.39
300_V318_I0.6440.39
335_I338_K0.6430.39
317_D332_K0.6390.39
29_V104_K0.6370.38
145_I159_E0.6330.38
122_V167_I0.6320.38
327_G341_E0.6320.38
329_L347_E0.6290.38
272_T321_A0.6290.38
152_P315_E0.6280.38
306_A335_I0.6260.37
222_A238_I0.6250.37
69_A96_K0.6190.37
263_N295_K0.6180.37
35_T281_I0.6180.37
14_P20_L0.6170.37
153_T187_L0.6160.37
276_I283_I0.6160.37
22_I58_I0.6150.36
91_Y121_V0.6100.36
263_N297_P0.6100.36
32_S56_C0.6090.36
73_A77_K0.6090.36
48_I75_I0.6080.36
168_L192_Y0.6040.35
146_L151_Y0.6020.35
45_V75_I0.6010.35
113_G118_V0.6010.35
50_R53_E0.6010.35
38_T48_I0.6000.35
114_R120_A0.5990.35
196_A203_L0.5990.35
8_H11_K0.5980.35
289_E346_E0.5980.35
44_T48_I0.5970.35
294_I353_D0.5950.35
64_P88_H0.5940.35
310_P313_A0.5910.34
336_V354_I0.5890.34
93_L241_S0.5890.34
340_P344_M0.5890.34
99_E127_D0.5890.34
83_L147_E0.5860.34
22_I83_L0.5860.34
243_D246_E0.5830.34
152_P159_E0.5820.34
197_K230_K0.5790.33
262_S297_P0.5790.33
182_A188_A0.5780.33
89_F306_A0.5780.33
329_L340_P0.5780.33
287_V344_M0.5760.33
122_V178_V0.5760.33
45_V83_L0.5760.33
192_Y203_L0.5760.33
163_H167_I0.5750.33
46_A53_E0.5750.33
232_I299_K0.5740.33
180_M192_Y0.5740.33
16_K129_G0.5740.33
44_T75_I0.5720.33
160_S183_S0.5700.33
73_A96_K0.5700.33
329_L345_V0.5700.33
184_D188_A0.5700.33
145_I151_Y0.5690.33
30_I227_I0.5670.32
182_A186_N0.5670.32
70_N346_E0.5670.32
343_T350_K0.5640.32
251_A350_K0.5640.32
196_A232_I0.5640.32
134_I192_Y0.5620.32
276_I301_A0.5610.32
72_I79_I0.5590.32
222_A229_S0.5580.32
177_I263_N0.5580.32
45_V81_I0.5560.32
259_G267_L0.5560.32
98_I128_K0.5540.31
283_I341_E0.5540.31
325_G339_V0.5530.31
142_E145_I0.5530.31
36_T75_I0.5520.31
224_L236_M0.5520.31
183_S219_K0.5510.31
76_K80_S0.5510.31
329_L337_R0.5500.31
76_K119_E0.5470.31
182_A207_I0.5470.31
130_I283_I0.5470.31
195_A203_L0.5460.31
39_H64_P0.5460.31
32_S243_D0.5450.31
91_Y113_G0.5430.31
296_A352_I0.5430.31
47_E244_P0.5420.30
167_I170_D0.5420.30
329_L336_V0.5420.30
28_V57_Q0.5420.30
113_G143_K0.5410.30
320_I329_L0.5410.30
214_F250_V0.5390.30
331_R355_L0.5390.30
150_G153_T0.5380.30
9_R12_T0.5380.30
238_I269_S0.5350.30
63_C102_A0.5350.30
177_I252_R0.5340.30
223_G238_I0.5340.30
267_L283_I0.5340.30
180_M188_A0.5340.30
90_D243_D0.5330.30
54_A249_K0.5320.30
61_V72_I0.5320.30
131_P176_I0.5310.30
123_K126_K0.5310.30
318_I331_R0.5300.30
298_I349_K0.5290.29
92_K117_K0.5290.29
166_K190_E0.5270.29
253_E267_L0.5270.29
156_G159_E0.5270.29
272_T278_I0.5250.29
122_V299_K0.5250.29
122_V126_K0.5240.29
131_P175_D0.5240.29
59_V81_I0.5240.29
276_I281_I0.5230.29
40_D44_T0.5220.29
278_I321_A0.5210.29
66_E93_L0.5210.29
138_A152_P0.5190.29
276_I280_L0.5190.29
141_L153_T0.5180.29
149_Y159_E0.5180.29
30_I152_P0.5170.29
81_I248_V0.5160.28
289_E334_K0.5160.28
72_I201_Y0.5160.28
34_T125_A0.5150.28
146_L149_Y0.5150.28
62_A88_H0.5150.28
105_I132_I0.5150.28
306_A326_E0.5140.28
184_D203_L0.5140.28
22_I26_N0.5130.28
134_I168_L0.5120.28
104_K175_D0.5120.28
36_T43_A0.5090.28
68_A93_L0.5080.28
205_L224_L0.5070.28
173_F176_I0.5060.28
40_D47_E0.5060.28
17_V175_D0.5050.28
107_I123_K0.5050.28
114_R117_K0.5040.28
118_V134_I0.5040.28
290_Y353_D0.5040.28
309_G322_G0.5040.28
184_D299_K0.5030.27
241_S303_L0.5030.27
350_K353_D0.5030.27
158_V287_V0.5030.27
104_K131_P0.5030.27
298_I317_D0.5020.27
113_G121_V0.5020.27
203_L232_I0.5010.27
331_R334_K0.5000.27
25_N42_E0.5000.27
186_N226_A0.5000.27
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4s39A 2 0.9416 100 0.255 Contact Map
3noyA 2 0.931 100 0.298 Contact Map
4mwaA 4 0.695 100 0.505 Contact Map
2yr1A 2 0.5942 100 0.74 Contact Map
4g38A 1 0.9125 99.8 0.866 Contact Map
1zj8A 1 0.9523 99.7 0.884 Contact Map
4nirA 3 0.6233 98.7 0.93 Contact Map
4djdD 1 0.6313 98.6 0.934 Contact Map
2yciX 2 0.634 98.5 0.936 Contact Map
4cczA 1 0.6844 98.4 0.938 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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