GREMLIN Database
ISPH - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
UniProt: P54473 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 314 (282)
Sequences: 2949 (1618)
Seq/√Len: 96.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
2_D263_T3.9021.00
122_H146_H3.4521.00
154_V183_L3.3181.00
121_Y163_K3.0721.00
250_L279_E2.9601.00
89_R93_E2.8721.00
217_L261_V2.8541.00
49_D52_E2.8321.00
138_V147_L2.8161.00
220_V267_T2.6811.00
151_E179_D2.6141.00
4_I214_K2.5591.00
218_T236_S2.4941.00
46_H249_D2.4631.00
113_I167_T2.3051.00
115_E119_K2.2981.00
151_E180_I2.2871.00
149_E153_D2.2861.00
49_D53_E2.2551.00
155_K183_L2.2191.00
116_M121_Y2.1661.00
253_L280_V2.1091.00
75_T97_V2.0421.00
89_R92_E2.0151.00
114_L118_E1.9971.00
155_K186_E1.9871.00
243_K261_V1.9651.00
283_F287_F1.9421.00
245_Y257_W1.9371.00
208_V236_S1.9271.00
4_I263_T1.9061.00
233_A237_E1.8971.00
210_E214_K1.8671.00
90_I93_E1.8331.00
25_A99_I1.8241.00
24_N28_D1.8141.00
223_D248_G1.8121.00
50_A54_E1.8091.00
26_S56_I1.8021.00
255_L287_F1.7851.00
250_L283_F1.7641.00
163_K190_H1.7531.00
23_K50_A1.7491.00
116_M165_I1.7441.00
258_L264_V1.7391.00
50_A275_P1.7361.00
217_L245_Y1.6991.00
183_L186_E1.6961.00
90_I94_K1.6911.00
215_A218_T1.6871.00
184_V191_V1.6721.00
283_F286_Q1.6411.00
254_K257_W1.6271.00
45_K49_D1.6131.00
122_H160_Q1.6111.00
212_A239_I1.5931.00
150_T153_D1.5871.00
247_I253_L1.5851.00
84_S139_G1.5851.00
219_I247_I1.5821.00
162_E190_H1.5711.00
52_E57_F1.5611.00
72_D94_K1.5591.00
152_E155_K1.5471.00
85_P114_L1.5431.00
229_S246_R1.5411.00
215_A265_A1.5191.00
213_K239_I1.4671.00
85_P139_G1.4621.00
23_K54_E1.4591.00
202_Q206_E1.4311.00
113_I137_A1.4251.00
249_D252_E1.4171.00
219_I253_L1.3890.99
154_V157_L1.3790.99
8_P207_A1.3690.99
22_A77_I1.3350.99
224_P252_E1.3300.99
221_V280_V1.3190.99
20_V24_N1.3180.99
63_N90_I1.3160.99
91_A96_L1.3140.99
151_E183_L1.3130.99
71_V74_G1.3040.99
50_A53_E1.3030.99
138_V145_V1.2990.99
3_V281_I1.2990.99
236_S244_A1.2900.99
175_W209_S1.2850.99
217_L258_L1.2450.99
34_P57_F1.2380.99
215_A263_T1.2360.99
3_V266_V1.2320.99
122_H144_I1.2320.99
126_I180_I1.2290.99
237_E243_K1.2270.99
129_K153_D1.2220.99
13_Y105_D1.2200.99
4_I215_A1.2180.98
47_V50_A1.2150.98
59_L70_Q1.2100.98
38_L87_V1.1800.98
210_E213_K1.1770.98
148_V157_L1.1760.98
154_V180_I1.1710.98
116_M119_K1.1710.98
216_D261_V1.1560.98
266_V281_I1.1530.98
111_N114_L1.1330.98
5_K281_I1.1320.98
70_Q73_K1.1290.98
124_I148_V1.1280.97
218_T265_A1.1260.97
200_A270_A1.1250.97
45_K223_D1.1170.97
125_Y167_T1.1080.97
22_A56_I1.1000.97
46_H275_P1.0980.97
166_V193_Y1.0960.97
256_E259_K1.0950.97
216_D262_N1.0940.97
128_K227_N1.0930.97
70_Q74_G1.0930.97
182_E186_E1.0920.97
37_I40_M1.0890.97
261_V264_V1.0880.97
116_M167_T1.0780.97
130_G135_E1.0740.97
28_D54_E1.0730.97
251_S279_E1.0680.97
128_K231_R1.0640.96
83_V87_V1.0630.96
2_D262_N1.0560.96
117_K141_A1.0520.96
224_P246_R1.0490.96
229_S269_G1.0470.96
217_L257_W1.0450.96
183_L187_K1.0410.96
117_K144_I1.0400.96
206_E210_E1.0370.96
258_L261_V1.0350.96
22_A37_I1.0290.96
45_K248_G1.0280.96
40_M225_K1.0280.96
85_P89_R1.0280.96
6_I211_Q1.0270.96
208_V267_T1.0200.95
85_P140_V1.0190.95
17_D21_I1.0150.95
219_I258_L1.0000.95
37_I56_I0.9980.95
231_R234_Q0.9910.95
125_Y134_P0.9900.95
110_H137_A0.9870.95
217_L264_V0.9840.94
166_V181_M0.9770.94
219_I257_W0.9750.94
188_Y191_V0.9670.94
72_D95_G0.9630.94
126_I166_V0.9580.94
2_D291_D0.9570.94
136_G139_G0.9540.94
121_Y192_E0.9490.93
7_S210_E0.9460.93
253_L258_L0.9450.93
178_H182_E0.9440.93
76_V91_A0.9410.93
150_T154_V0.9390.93
146_H157_L0.9390.93
121_Y190_H0.9380.93
282_R286_Q0.9370.93
40_M52_E0.9330.93
208_V218_T0.9310.93
237_E241_G0.9270.93
9_R207_A0.9210.92
168_N177_V0.9140.92
124_I157_L0.9140.92
209_S235_V0.9120.92
7_S207_A0.9040.92
39_G79_T0.9040.92
209_S238_E0.8940.91
39_G132_P0.8910.91
274_T278_K0.8880.91
108_K112_L0.8850.91
200_A207_A0.8820.91
230_N233_A0.8750.90
268_A277_T0.8710.90
129_K149_E0.8710.90
88_R140_V0.8700.90
255_L283_F0.8610.89
85_P88_R0.8600.89
84_S88_R0.8550.89
200_A267_T0.8530.89
33_R75_T0.8480.89
62_S91_A0.8470.89
282_R285_E0.8440.89
116_M192_E0.8390.88
23_K51_F0.8340.88
249_D279_E0.8330.88
74_G96_L0.8280.88
113_I123_V0.8270.88
172_M176_D0.8260.87
233_A244_A0.8260.87
21_I24_N0.8230.87
86_E89_R0.8200.87
46_H49_D0.8180.87
281_I285_E0.8150.87
182_E185_K0.8140.87
253_L283_F0.8120.87
152_E156_N0.8110.86
34_P74_G0.8090.86
86_E90_I0.8080.86
119_K163_K0.8070.86
19_M47_V0.7970.86
219_I245_Y0.7960.85
26_S54_E0.7920.85
130_G147_L0.7920.85
172_M227_N0.7870.85
112_L194_H0.7870.85
88_R100_D0.7840.85
41_I99_I0.7820.84
222_G269_G0.7810.84
151_E155_K0.7800.84
221_V247_I0.7760.84
158_D161_S0.7680.83
254_K258_L0.7670.83
84_S140_V0.7670.83
246_R253_L0.7650.83
16_V20_V0.7630.83
122_H157_L0.7620.83
76_V96_L0.7620.83
227_N231_R0.7610.83
125_Y145_V0.7570.82
121_Y165_I0.7560.82
117_K142_P0.7550.82
123_V165_I0.7540.82
122_H162_E0.7510.82
84_S135_E0.7490.82
224_P248_G0.7470.82
279_E284_L0.7460.82
124_I164_L0.7440.81
41_I195_Q0.7440.81
11_Y19_M0.7430.81
123_V167_T0.7430.81
166_V184_V0.7390.81
284_L288_D0.7380.81
105_D199_L0.7320.80
112_L165_I0.7300.80
35_I56_I0.7290.80
22_A51_F0.7280.80
105_D231_R0.7260.80
201_T205_Q0.7240.80
155_K187_K0.7240.80
42_V80_A0.7240.80
222_G229_S0.7200.79
125_Y147_L0.7200.79
19_M51_F0.7190.79
109_T227_N0.7170.79
46_H223_D0.7160.79
109_T137_A0.7110.78
88_R107_T0.7070.78
233_A246_R0.7070.78
6_I265_A0.7070.78
62_S90_I0.7030.78
152_E251_S0.7020.77
221_V266_V0.6990.77
45_K225_K0.6980.77
179_D182_E0.6980.77
236_S242_T0.6980.77
118_E142_P0.6960.77
214_K263_T0.6960.77
122_H145_V0.6940.77
117_K123_V0.6920.76
141_A144_I0.6910.76
3_V285_E0.6910.76
266_V280_V0.6910.76
5_K266_V0.6880.76
37_I58_T0.6850.76
247_I280_V0.6790.75
13_Y128_K0.6730.74
84_S98_A0.6690.74
125_Y138_V0.6680.74
200_A205_Q0.6660.74
174_Q195_Q0.6660.74
209_S239_I0.6660.74
6_I16_V0.6630.73
110_H136_G0.6610.73
147_L153_D0.6610.73
22_A41_I0.6600.73
7_S41_I0.6590.73
113_I141_A0.6580.73
148_V153_D0.6480.72
30_S75_T0.6470.72
105_D110_H0.6450.71
78_F91_A0.6430.71
161_S190_H0.6410.71
8_P203_V0.6410.71
62_S70_Q0.6390.71
257_W260_G0.6390.71
10_G14_G0.6350.70
119_K192_E0.6340.70
223_D229_S0.6320.70
26_S35_I0.6310.70
255_L258_L0.6310.70
10_G15_V0.6270.69
25_A77_I0.6270.69
266_V288_D0.6270.69
207_A274_T0.6260.69
219_I246_R0.6250.69
217_L254_K0.6240.69
237_E242_T0.6190.68
176_D180_I0.6190.68
109_T170_T0.6170.68
69_K94_K0.6160.68
36_Y57_F0.6150.68
239_I242_T0.6150.68
126_I176_D0.6130.67
168_N172_M0.6120.67
40_M45_K0.6110.67
92_E95_G0.6110.67
233_A258_L0.6100.67
12_C15_V0.6100.67
199_L203_V0.6090.67
209_S213_K0.6090.67
155_K182_E0.6090.67
122_H161_S0.6070.67
111_N115_E0.6070.67
41_I48_T0.6050.66
207_A211_Q0.6030.66
120_G160_Q0.6010.66
117_K145_V0.6010.66
128_K149_E0.5990.66
37_I101_A0.5980.66
278_K284_L0.5980.66
36_Y74_G0.5960.65
146_H159_I0.5960.65
211_Q218_T0.5930.65
85_P141_A0.5890.64
141_A145_V0.5860.64
117_K125_Y0.5850.64
42_V102_T0.5840.64
230_N246_R0.5840.64
80_A140_V0.5830.64
6_I221_V0.5830.64
232_L267_T0.5820.63
45_K252_E0.5800.63
84_S145_V0.5790.63
83_V88_R0.5790.63
116_M163_K0.5790.63
164_L191_V0.5740.62
248_G252_E0.5710.62
157_L187_K0.5710.62
23_K26_S0.5710.62
200_A268_A0.5710.62
17_D22_A0.5700.62
278_K285_E0.5680.62
123_V141_A0.5680.62
3_V264_V0.5680.62
134_P137_A0.5660.61
130_G140_V0.5660.61
201_T231_R0.5660.61
205_Q270_A0.5660.61
16_V274_T0.5650.61
117_K140_V0.5620.61
126_I177_V0.5620.61
245_Y256_E0.5620.61
70_Q76_V0.5620.61
116_M125_Y0.5620.61
105_D128_K0.5590.60
146_H158_D0.5590.60
126_I172_M0.5580.60
201_T232_L0.5580.60
42_V105_D0.5550.60
153_D156_N0.5540.60
53_E161_S0.5540.60
91_A98_A0.5500.59
275_P279_E0.5490.59
22_A26_S0.5480.59
130_G149_E0.5460.59
79_T177_V0.5450.58
148_V154_V0.5430.58
138_V143_E0.5430.58
212_A242_T0.5420.58
73_K94_K0.5420.58
48_T236_S0.5420.58
31_L75_T0.5410.58
186_E190_H0.5410.58
132_P167_T0.5400.58
122_H164_L0.5400.58
9_R136_G0.5370.57
185_K190_H0.5360.57
10_G211_Q0.5350.57
63_N69_K0.5330.57
136_G172_M0.5320.57
36_Y59_L0.5280.56
20_V23_K0.5270.56
163_K247_I0.5260.56
5_K202_Q0.5230.55
100_D107_T0.5220.55
111_N119_K0.5210.55
269_G277_T0.5210.55
177_V181_M0.5200.55
28_D286_Q0.5190.55
11_Y15_V0.5190.55
99_I113_I0.5190.55
123_V194_H0.5180.55
15_V42_V0.5160.54
4_I211_Q0.5150.54
75_T99_I0.5140.54
10_G274_T0.5130.54
154_V184_V0.5120.54
257_W280_V0.5120.54
113_I165_I0.5120.54
126_I168_N0.5110.54
185_K192_E0.5110.54
89_R239_I0.5100.54
201_T270_A0.5080.53
125_Y196_E0.5040.53
158_D187_K0.5040.53
26_S55_G0.5030.53
59_L76_V0.5020.52
9_R110_H0.5000.52
9_R268_A0.5000.52
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4n7bA 1 0.9745 100 0.052 Contact Map
4h4dA 1 0.9682 100 0.054 Contact Map
3dnfA 2 0.8854 100 0.084 Contact Map
4toiA 2 0.7102 100 0.696 Contact Map
3o3mB 1 0.5223 68.3 0.961 Contact Map
4jenA 2 0.3535 66.6 0.961 Contact Map
4oo4A 2 0.3344 57.5 0.963 Contact Map
3wgdA 3 0.3439 55.7 0.964 Contact Map
3aekA 1 0.8057 55.4 0.964 Contact Map
4bw0B 1 0.3567 53.1 0.964 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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