GREMLIN Database
CWLH - N-acetylmuramoyl-L-alanine amidase CwlH
UniProt: P54450 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 250 (212)
Sequences: 1287 (860)
Seq/√Len: 59.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
27_T129_V5.0591.00
77_H92_G4.4831.00
211_D215_G3.8611.00
95_I110_A3.8481.00
191_Q218_T3.7251.00
26_I114_I3.4841.00
191_Q212_G3.4731.00
148_N152_N3.4681.00
111_Q153_G2.9761.00
70_P73_E2.6111.00
60_V110_A2.5961.00
107_T145_D2.3621.00
212_G218_T2.3361.00
33_N46_F2.2991.00
69_L75_G2.1171.00
187_I191_Q2.0971.00
26_I118_M2.0931.00
104_E149_S2.0351.00
226_Q235_G2.0051.00
60_V95_I2.0031.00
71_I120_E1.9941.00
58_F71_I1.9671.00
71_I117_L1.9331.00
197_L229_N1.9291.00
28_V110_A1.9071.00
214_Y218_T1.8931.00
58_F113_L1.8801.00
215_G218_T1.8681.00
149_S153_G1.8491.00
114_I128_V1.8451.00
118_M128_V1.8451.00
190_L237_Y1.7890.99
41_K45_K1.7820.99
81_G134_W1.7330.99
42_M46_F1.7280.99
240_K244_K1.6930.99
43_H47_V1.6610.99
178_T181_Y1.6610.99
197_L245_L1.6380.99
55_S69_L1.5940.99
26_I128_V1.5600.98
180_P219_A1.5560.98
28_V114_I1.5490.98
150_F154_I1.5240.98
219_A223_K1.5020.98
184_G187_I1.4940.98
65_I109_N1.4900.98
107_T153_G1.4530.98
181_Y184_G1.4520.98
191_Q215_G1.4320.97
130_P133_K1.4190.97
56_W94_E1.3950.97
116_K120_E1.3920.97
214_Y237_Y1.3890.97
193_A246_K1.3800.97
27_T134_W1.3680.96
23_P121_N1.3670.96
216_P220_N1.3640.96
31_T94_E1.3450.96
211_D218_T1.3000.95
147_W151_L1.2980.95
108_S112_W1.2870.95
16_R19_Y1.2690.95
159_T164_T1.2470.94
86_G134_W1.2370.94
47_V59_T1.2120.93
117_L120_E1.2050.93
218_T221_A1.2000.93
14_D73_E1.1940.93
111_Q115_R1.1830.92
174_V178_T1.1790.92
197_L228_M1.1790.92
187_I218_T1.1790.92
190_L222_V1.1600.91
60_V65_I1.1560.91
142_K227_L1.1520.91
110_A150_F1.1280.90
46_F50_P1.1240.90
129_V134_W1.1190.90
30_N97_E1.1130.90
193_A196_V1.0980.89
133_K147_W1.0950.89
104_E108_S1.0920.89
136_G139_C1.0880.89
65_I113_L1.0780.88
221_A245_L1.0680.88
44_A47_V1.0570.87
174_V177_L1.0460.87
239_P243_A1.0450.86
43_H46_F1.0410.86
178_T187_I1.0410.86
20_A74_N1.0330.86
59_T68_H1.0250.85
151_L154_I1.0230.85
223_K227_L1.0220.85
195_A209_G1.0090.85
226_Q234_D1.0000.84
78_A92_G0.9990.84
30_N137_K0.9970.84
208_N221_A0.9960.84
25_Y90_S0.9880.83
214_Y222_V0.9870.83
130_P144_L0.9870.83
47_V68_H0.9850.83
25_Y86_G0.9800.83
130_P140_P0.9750.82
124_P127_R0.9720.82
32_A100_D0.9640.82
45_K49_N0.9630.82
85_T88_R0.9550.81
214_Y219_A0.9510.81
91_I117_L0.9420.80
68_H139_C0.9390.80
61_D96_C0.9380.80
82_T200_Y0.9360.80
191_Q211_D0.9350.80
156_S159_T0.9350.80
186_N193_A0.9300.79
237_Y242_K0.9190.79
47_V55_S0.9100.78
31_T137_K0.9100.78
154_I158_D0.9070.78
29_H131_H0.9050.77
32_A43_H0.9040.77
217_K223_K0.9030.77
24_A89_K0.9020.77
188_L210_I0.9010.77
75_G92_G0.8990.77
28_V95_I0.8960.77
111_Q154_I0.8850.76
156_S228_M0.8780.75
230_G244_K0.8680.74
60_V93_I0.8590.74
147_W150_F0.8550.73
139_C144_L0.8510.73
231_L237_Y0.8500.73
30_N141_R0.8410.72
194_L198_H0.8370.72
173_G177_L0.8370.72
130_P139_C0.8310.71
106_A144_L0.8300.71
17_P20_A0.8190.70
185_T188_L0.8180.70
123_I127_R0.8110.69
74_N88_R0.8080.69
16_R20_A0.8030.69
93_I113_L0.8000.68
65_I76_W0.8000.68
159_T244_K0.7980.68
222_V237_Y0.7980.68
176_K184_G0.7940.68
122_N223_K0.7930.68
210_I213_V0.7930.68
209_G217_K0.7880.67
55_S76_W0.7860.67
30_N99_A0.7860.67
177_L185_T0.7790.66
46_F52_T0.7740.66
98_N101_G0.7730.66
82_T85_T0.7680.65
222_V241_T0.7630.65
97_E106_A0.7630.65
230_G240_K0.7570.64
78_A90_S0.7550.64
220_N239_P0.7520.63
63_S109_N0.7490.63
186_N189_Q0.7450.63
177_L181_Y0.7450.63
77_H90_S0.7400.62
106_A130_P0.7380.62
231_L240_K0.7370.62
81_G86_G0.7330.61
32_A98_N0.7270.61
164_T195_A0.7270.61
15_N19_Y0.7240.60
177_L180_P0.7240.60
192_K196_V0.7240.60
154_I159_T0.7230.60
210_I214_Y0.7220.60
96_C106_A0.7210.60
69_L76_W0.7210.60
137_K141_R0.7150.60
223_K233_A0.7150.60
118_M155_S0.7120.59
97_E139_C0.7040.58
73_E121_N0.7040.58
79_G135_S0.7020.58
230_G238_G0.6940.57
242_K245_L0.6920.57
229_N244_K0.6920.57
187_I214_Y0.6920.57
234_D241_T0.6850.56
132_K136_G0.6840.56
54_E73_E0.6800.56
128_V154_I0.6800.56
200_Y217_K0.6790.56
55_S90_S0.6790.56
213_V216_P0.6770.55
227_L233_A0.6770.55
44_A48_K0.6690.55
216_P232_T0.6640.54
178_T185_T0.6620.54
24_A85_T0.6610.54
57_H87_N0.6590.53
23_P72_D0.6580.53
47_V95_I0.6570.53
56_W69_L0.6530.53
76_W94_E0.6530.53
122_N192_K0.6520.53
182_R187_I0.6500.52
97_E103_F0.6490.52
224_R228_M0.6480.52
46_F49_N0.6480.52
179_S182_R0.6470.52
187_I212_G0.6460.52
118_M124_P0.6460.52
237_Y241_T0.6450.52
226_Q233_A0.6450.52
200_Y209_G0.6430.52
55_S137_K0.6420.52
211_D214_Y0.6420.52
65_I162_K0.6410.51
26_I117_L0.6370.51
114_I155_S0.6350.51
108_S153_G0.6350.51
155_S159_T0.6330.51
15_N53_S0.6320.50
58_F147_W0.6290.50
210_I242_K0.6260.50
226_Q241_T0.6210.49
191_Q210_I0.6190.49
212_G215_G0.6190.49
177_L184_G0.6180.49
75_G94_E0.6180.49
176_K185_T0.6170.49
100_D142_K0.6160.49
56_W68_H0.6100.48
30_N147_W0.6100.48
16_R73_E0.6090.48
180_P214_Y0.6090.48
71_I113_L0.6090.48
133_K141_R0.6080.48
32_A142_K0.6010.47
188_L192_K0.6000.47
191_Q214_Y0.5980.47
81_G137_K0.5950.46
242_K246_K0.5950.46
177_L242_K0.5930.46
77_H137_K0.5910.46
23_P89_K0.5890.46
199_F228_M0.5890.46
25_Y89_K0.5880.45
185_T242_K0.5880.45
191_Q221_A0.5880.45
31_T79_G0.5880.45
78_A134_W0.5870.45
193_A242_K0.5870.45
118_M158_D0.5840.45
130_P141_R0.5840.45
90_S123_I0.5800.45
141_R144_L0.5740.44
62_D98_N0.5650.43
219_A233_A0.5640.43
61_D64_V0.5630.43
43_H96_C0.5610.43
128_V155_S0.5600.42
136_G144_L0.5600.42
219_A225_F0.5600.42
55_S58_F0.5600.42
220_N223_K0.5580.42
233_A237_Y0.5570.42
179_S236_I0.5560.42
91_I120_E0.5550.42
33_N42_M0.5550.42
69_L97_E0.5530.42
19_Y73_E0.5520.42
31_T150_F0.5500.41
229_N238_G0.5500.41
141_R150_F0.5480.41
189_Q246_K0.5480.41
43_H59_T0.5470.41
33_N102_D0.5450.41
95_I106_A0.5430.41
227_L237_Y0.5410.40
68_H78_A0.5390.40
76_W79_G0.5380.40
157_S197_L0.5360.40
195_A210_I0.5360.40
54_E76_W0.5350.40
107_T111_Q0.5330.40
99_A106_A0.5330.40
27_T90_S0.5330.40
189_Q193_A0.5280.39
75_G97_E0.5270.39
208_N227_L0.5260.39
55_S75_G0.5250.39
157_S160_P0.5240.39
26_I130_P0.5230.38
33_N43_H0.5220.38
19_Y22_A0.5210.38
74_N85_T0.5200.38
225_F229_N0.5190.38
209_G212_G0.5170.38
55_S94_E0.5160.38
55_S97_E0.5150.38
141_R145_D0.5130.37
67_Q113_L0.5120.37
20_A128_V0.5120.37
54_E68_H0.5120.37
59_T96_C0.5090.37
187_I215_G0.5080.37
137_K147_W0.5060.37
33_N100_D0.5040.36
132_K141_R0.5040.36
15_N18_G0.5010.36
183_K216_P0.5010.36
177_L189_Q0.5010.36
115_R158_D0.5000.36
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3d2yA 3 0.904 100 0.297 Contact Map
4bolA 2 0.892 100 0.302 Contact Map
4bxjA 4 0.884 100 0.304 Contact Map
3rdrA 4 0.612 100 0.466 Contact Map
1yb0A 3 0.616 100 0.467 Contact Map
4knkA 1 0.66 100 0.476 Contact Map
4ivvA 1 0.6 100 0.486 Contact Map
4olsA 2 0.616 100 0.492 Contact Map
1j3gA 1 0.644 100 0.519 Contact Map
1ohtA 1 0.616 100 0.557 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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