GREMLIN Database
EX53 - 5'-3' exonuclease
UniProt: P54161 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 296 (277)
Sequences: 4550 (2859)
Seq/√Len: 171.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
221_N224_L4.4731.00
165_T168_T3.9031.00
32_D279_Q3.1641.00
120_G249_I3.1221.00
231_G235_Q3.0251.00
236_G239_D2.9131.00
74_L245_K2.8911.00
237_L241_E2.7461.00
198_K236_G2.7241.00
127_A146_T2.7211.00
176_M179_A2.6311.00
219_L241_E2.4231.00
5_K131_D2.3681.00
230_Q234_Q2.3611.00
97_K109_G2.3511.00
170_Y176_M2.3371.00
101_A107_N2.3221.00
267_Q270_Q2.2881.00
31_N279_Q2.1501.00
228_G232_K2.1441.00
214_T217_R2.1171.00
228_G231_G2.0981.00
219_L237_L2.0811.00
124_D251_C2.0811.00
217_R220_E2.0201.00
11_G61_C1.9821.00
216_D241_E1.9591.00
98_E277_R1.9411.00
147_D166_K1.9371.00
49_T53_T1.8731.00
183_I209_I1.8201.00
216_D244_R1.8191.00
167_E171_E1.8101.00
258_T261_D1.7871.00
272_A284_E1.7821.00
200_I229_Q1.7721.00
119_I134_V1.7641.00
211_E224_L1.7591.00
166_K178_K1.7581.00
33_S277_R1.7541.00
217_R221_N1.7341.00
44_L99_A1.7291.00
194_Y247_A1.6831.00
227_K230_Q1.6511.00
167_E170_Y1.6461.00
219_L222_L1.6381.00
98_E101_A1.6261.00
245_K250_H1.6081.00
85_P90_I1.6081.00
68_K73_D1.6031.00
197_V240_L1.6001.00
274_M278_H1.5951.00
222_L234_Q1.5951.00
67_S111_A1.5711.00
183_I187_M1.5671.00
31_N35_V1.5281.00
124_D128_N1.5191.00
207_K211_E1.5191.00
65_M111_A1.5181.00
197_V233_I1.4921.00
19_F88_E1.4811.00
123_A149_V1.4781.00
35_V95_L1.4761.00
131_D148_K1.4761.00
225_L233_I1.4761.00
102_E274_M1.4641.00
272_A276_R1.4551.00
199_G232_K1.4271.00
182_D244_R1.4231.00
175_V206_Y1.4091.00
50_A135_V1.4051.00
16_F20_F1.3831.00
87_V90_I1.3771.00
136_T141_L1.3681.00
145_L169_F1.3571.00
166_K170_Y1.3511.00
142_L151_V1.3461.00
187_M202_E1.3451.00
282_G285_R1.3341.00
219_L233_I1.3321.00
244_R248_E1.3181.00
5_K133_T1.3161.00
216_D220_E1.3061.00
127_A149_V1.3061.00
120_G251_C1.2981.00
15_L96_A1.2981.00
181_I248_E1.2961.00
7_L135_V1.2901.00
73_D112_G1.2821.00
18_A46_H1.2741.00
232_K235_Q1.2691.00
150_S163_V1.2501.00
60_V262_A1.2501.00
168_T171_E1.2491.00
68_K72_N1.2481.00
75_F246_L1.2311.00
222_L230_Q1.2251.00
185_A247_A1.2251.00
22_T38_N1.2231.00
113_Y250_H1.2161.00
74_L242_M1.2141.00
36_P91_P1.2071.00
45_K286_M1.2061.00
121_T253_V1.2001.00
4_N57_T1.1991.00
206_Y210_R1.1971.00
270_Q273_D1.1931.00
212_Y221_N1.1851.00
174_G210_R1.1581.00
81_N193_N1.1571.00
273_D277_R1.1521.00
69_T72_N1.1461.00
62_C118_C1.1441.00
102_E270_Q1.1331.00
145_L151_V1.1241.00
219_L240_L1.1211.00
186_L204_T1.1191.00
113_Y253_V1.1191.00
227_K231_G1.1051.00
47_L105_I1.1021.00
110_F118_C1.1011.00
179_A213_E1.0981.00
194_Y243_S1.0941.00
212_Y225_L1.0841.00
35_V91_P1.0801.00
212_Y224_L1.0791.00
250_H253_V1.0771.00
94_D98_E1.0731.00
208_L218_L1.0661.00
125_L129_E1.0581.00
123_A132_I1.0581.00
35_V277_R1.0571.00
40_V278_H1.0480.99
139_R184_K1.0450.99
169_F175_V1.0430.99
126_F132_I1.0400.99
198_K239_D1.0400.99
232_K236_G1.0350.99
99_A274_M1.0340.99
164_Y168_T1.0300.99
87_V91_P1.0280.99
65_M109_G1.0270.99
63_W107_N1.0220.99
231_G234_Q1.0130.99
127_A148_K1.0060.99
52_E286_M1.0040.99
113_Y254_P1.0040.99
131_D150_S1.0010.99
269_E273_D1.0000.99
276_R284_E0.9960.99
7_L50_A0.9930.99
183_I202_E0.9800.99
132_I149_V0.9670.99
124_D146_T0.9670.99
6_L126_F0.9650.99
273_D276_R0.9520.99
48_I275_L0.9500.99
275_L283_I0.9420.99
7_L59_V0.9360.99
213_E217_R0.9330.99
99_A278_H0.9280.99
20_F83_S0.9250.99
41_N45_K0.9240.99
170_Y210_R0.9210.99
124_D127_A0.9110.99
162_K172_E0.9100.99
15_L89_L0.9050.98
36_P88_E0.9030.98
9_V61_C0.9030.98
103_L271_A0.8970.98
202_E206_Y0.8970.98
226_P229_Q0.8900.98
41_N280_I0.8860.98
268_M272_A0.8840.98
65_M68_K0.8830.98
152_A163_V0.8800.98
181_I247_A0.8770.98
239_D242_M0.8770.98
142_L164_Y0.8770.98
162_K168_T0.8760.98
195_P243_S0.8710.98
243_S247_A0.8680.98
234_Q237_L0.8630.98
168_T172_E0.8510.98
266_L271_A0.8470.98
4_N58_H0.8430.98
39_G92_Q0.8410.98
183_I206_Y0.8410.98
260_K263_L0.8380.97
75_F195_P0.8360.97
133_T152_A0.8360.97
6_L262_A0.8340.97
104_G267_Q0.8330.97
67_S72_N0.8260.97
241_E244_R0.8260.97
222_L233_I0.8230.97
119_I141_L0.8230.97
16_F85_P0.8220.97
40_V96_A0.8210.97
141_L249_I0.8190.97
215_I240_L0.8180.97
281_K285_R0.8140.97
185_A240_L0.8110.97
22_T42_G0.8090.97
88_E91_P0.8060.97
275_L280_I0.8040.97
7_L54_F0.7990.97
44_L96_A0.7980.97
204_T207_K0.7980.97
75_F242_M0.7960.97
98_E270_Q0.7960.97
54_F133_T0.7930.96
39_G96_A0.7910.96
59_V264_F0.7880.96
127_A132_I0.7850.96
257_C262_A0.7840.96
166_K176_M0.7820.96
43_F100_A0.7810.96
272_A288_E0.7770.96
200_I208_L0.7770.96
48_I283_I0.7730.96
179_A209_I0.7730.96
93_F97_K0.7720.96
248_E251_C0.7670.96
40_V95_L0.7660.96
41_N282_G0.7640.96
211_E226_P0.7630.96
54_F163_V0.7610.96
142_L145_L0.7590.96
207_K229_Q0.7580.95
77_D80_A0.7570.95
175_V183_I0.7550.95
186_L233_I0.7540.95
208_L229_Q0.7510.95
7_L133_T0.7490.95
20_F86_P0.7480.95
229_Q232_K0.7480.95
199_G228_G0.7480.95
164_Y172_E0.7470.95
237_L240_L0.7410.95
175_V179_A0.7410.95
238_S241_E0.7410.95
101_A270_Q0.7400.95
68_K112_G0.7300.94
80_A193_N0.7300.94
61_C100_A0.7290.94
6_L132_I0.7280.94
169_F180_L0.7280.94
271_A275_L0.7270.94
43_F96_A0.7250.94
222_L237_L0.7230.94
8_L132_I0.7220.94
9_V14_L0.7190.94
153_L156_K0.7180.94
175_V180_L0.7170.94
221_N225_L0.7170.94
23_A38_N0.7150.94
204_T229_Q0.7150.94
133_T163_V0.7150.94
185_A243_S0.7110.94
225_L229_Q0.7110.94
241_E245_K0.7100.94
13_A154_L0.7100.94
126_F130_A0.7090.94
240_L243_S0.7070.93
180_L247_A0.7070.93
200_I204_T0.7060.93
95_L278_H0.7020.93
169_F172_E0.7010.93
8_L122_L0.7000.93
44_L48_I0.6970.93
132_I148_K0.6950.93
65_M118_C0.6940.93
70_Y250_H0.6930.93
173_T180_L0.6930.93
223_S234_Q0.6920.93
91_P94_D0.6870.92
142_L153_L0.6860.92
108_I122_L0.6860.92
95_L98_E0.6830.92
59_V105_I0.6830.92
24_V38_N0.6780.92
19_F23_A0.6770.92
23_A42_G0.6760.92
16_F86_P0.6760.92
106_M261_D0.6740.92
84_A87_V0.6710.92
133_T150_S0.6690.91
139_R153_L0.6680.91
153_L184_K0.6660.91
150_S165_T0.6580.91
197_V236_G0.6550.91
185_A215_I0.6550.91
61_C105_I0.6460.90
99_A275_L0.6460.90
185_A244_R0.6450.90
31_N278_H0.6420.90
282_G286_M0.6420.90
197_V232_K0.6410.90
125_L258_T0.6400.90
215_I218_L0.6400.90
16_F83_S0.6390.90
180_L184_K0.6380.89
13_A136_T0.6340.89
146_T149_V0.6320.89
186_L215_I0.6320.89
66_G90_I0.6300.89
58_H263_L0.6300.89
62_C122_L0.6290.89
215_I241_E0.6250.88
48_I286_M0.6240.88
125_L128_N0.6230.88
236_G240_L0.6210.88
54_F152_A0.6200.88
276_R281_K0.6200.88
222_L225_L0.6190.88
218_L225_L0.6160.88
223_S230_Q0.6140.88
102_E271_A0.6140.88
183_I205_A0.6080.87
179_A182_D0.6070.87
135_V152_A0.6060.87
51_V264_F0.6060.87
140_D192_D0.6060.87
242_M245_K0.6060.87
8_L119_I0.6040.87
24_V88_E0.6020.87
46_H154_L0.6020.87
170_Y174_G0.6000.86
51_V59_V0.6000.86
129_E260_K0.5990.86
29_M34_G0.5990.86
11_G15_L0.5980.86
123_A127_A0.5940.86
179_A214_T0.5930.86
181_I244_R0.5930.86
13_A137_G0.5930.86
5_K57_T0.5910.86
5_K54_F0.5900.85
63_W109_G0.5890.85
169_F206_Y0.5890.85
123_A251_C0.5860.85
198_K235_Q0.5850.85
233_I240_L0.5850.85
55_Q264_F0.5840.85
110_F254_P0.5830.85
69_T73_D0.5830.85
33_S279_Q0.5820.85
209_I213_E0.5820.85
276_R288_E0.5790.84
266_L287_L0.5780.84
184_K191_S0.5770.84
180_L183_I0.5760.84
12_M63_W0.5740.84
218_L221_N0.5740.84
175_V210_R0.5720.84
176_M213_E0.5710.84
142_L180_L0.5700.83
24_V30_I0.5690.83
12_M93_F0.5660.83
41_N283_I0.5660.83
48_I103_L0.5620.83
150_S166_K0.5610.83
19_F24_V0.5610.83
104_G266_L0.5610.83
166_K177_P0.5600.82
152_A161_Y0.5580.82
145_L166_K0.5570.82
181_I249_I0.5560.82
174_G206_Y0.5550.82
181_I185_A0.5540.82
225_L230_Q0.5540.82
227_K234_Q0.5530.82
72_N191_S0.5520.81
104_G270_Q0.5510.81
8_L60_V0.5510.81
245_K248_E0.5510.81
169_F173_T0.5450.81
19_F36_P0.5440.81
209_I214_T0.5430.80
95_L277_R0.5420.80
218_L222_L0.5410.80
60_V108_I0.5390.80
70_Y113_Y0.5380.80
110_F113_Y0.5360.80
14_L141_L0.5350.79
8_L134_V0.5350.79
108_I262_A0.5340.79
183_I200_I0.5340.79
198_K232_K0.5320.79
145_L168_T0.5290.79
280_I283_I0.5290.79
186_L218_L0.5290.79
187_M209_I0.5290.79
110_F253_V0.5250.78
274_M277_R0.5250.78
14_L161_Y0.5240.78
46_H49_T0.5220.78
85_P89_L0.5210.78
189_D192_D0.5210.78
44_L275_L0.5170.77
186_L197_V0.5170.77
214_T220_E0.5150.77
285_R288_E0.5150.77
207_K227_K0.5130.77
63_W97_K0.5120.76
272_A281_K0.5110.76
141_L180_L0.5110.76
235_Q239_D0.5100.76
14_L135_V0.5100.76
9_V135_V0.5080.76
226_P231_G0.5060.76
84_A90_I0.5050.75
206_Y209_I0.5030.75
212_Y217_R0.5010.75
40_V99_A0.5000.75
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1bgxT 1 0.9628 100 0.197 Contact Map
1exnA 1 0.8682 100 0.275 Contact Map
3zdbA 1 0.8209 100 0.283 Contact Map
3h7iA 1 0.8986 100 0.284 Contact Map
2izoA 1 0.7601 100 0.411 Contact Map
4q0wA 1 0.9155 100 0.42 Contact Map
1rxwA 1 0.8615 100 0.421 Contact Map
3oryA 3 0.9155 100 0.424 Contact Map
3q8kA 1 0.9392 100 0.435 Contact Map
4wa8A 1 0.8547 100 0.441 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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