GREMLIN Database
BIOB - Biotin synthase
UniProt: P53557 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 335 (305)
Sequences: 5942 (3549)
Seq/√Len: 203.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
149_P195_E4.3491.00
171_Q184_D4.3131.00
172_R214_D3.1191.00
41_F45_K3.0461.00
123_D154_R3.0461.00
19_D217_D3.0181.00
170_S214_D2.9451.00
68_N77_I2.9241.00
43_I263_F2.7521.00
126_V154_R2.6751.00
89_N92_T2.6701.00
271_R292_N2.6451.00
230_I265_N2.6061.00
149_P153_K2.5631.00
191_E228_D2.5421.00
122_V126_V2.5301.00
149_P192_I2.5181.00
23_L213_Q2.3311.00
220_K264_I2.3131.00
188_N192_I2.2281.00
19_D220_K2.2011.00
184_D188_N2.1781.00
153_K157_D2.0681.00
51_K320_E2.0501.00
95_E98_K1.9741.00
202_S222_L1.9511.00
212_K253_Y1.9441.00
215_V253_Y1.8991.00
98_K102_D1.8891.00
25_I260_L1.8611.00
167_L218_I1.8541.00
156_K196_S1.8121.00
90_K131_E1.7851.00
18_T21_E1.7831.00
172_R217_D1.7791.00
22_A264_I1.7791.00
90_K127_D1.7621.00
98_K132_I1.7581.00
39_G263_F1.7491.00
90_K124_Q1.7341.00
240_G243_L1.7231.00
94_L131_E1.7031.00
23_L27_H1.7031.00
150_E153_K1.6891.00
32_D35_L1.6721.00
92_T95_E1.6671.00
219_A261_F1.6661.00
94_L98_K1.6641.00
162_R199_S1.6561.00
45_K50_K1.6181.00
300_L304_G1.6121.00
240_G244_E1.5871.00
93_L128_A1.5821.00
23_L216_I1.5751.00
50_K320_E1.5651.00
41_F44_R1.5571.00
130_Q161_D1.5371.00
148_K151_Q1.5331.00
156_K198_L1.5301.00
152_A196_S1.5291.00
19_D216_I1.5241.00
173_N176_N1.5231.00
270_I291_A1.5111.00
99_R102_D1.5031.00
191_E195_E1.5031.00
185_D188_N1.5021.00
37_M290_A1.4981.00
100_A105_I1.4901.00
215_V257_V1.4761.00
237_A248_E1.4751.00
19_D213_Q1.4631.00
285_P316_D1.4601.00
42_H46_H1.4541.00
307_E311_H1.4431.00
182_T185_D1.4431.00
308_T312_K1.4391.00
306_E309_E1.4351.00
19_D23_L1.4261.00
41_F289_Y1.4111.00
312_K316_D1.3971.00
17_V263_F1.3901.00
147_L155_L1.3791.00
54_L311_H1.3701.00
27_H212_K1.3681.00
255_L287_G1.3621.00
154_R157_D1.3571.00
240_G245_G1.3471.00
119_N151_Q1.3461.00
119_N154_R1.3421.00
281_R316_D1.3411.00
311_H315_S1.3231.00
12_L42_H1.3231.00
126_V130_Q1.3211.00
272_I287_G1.3211.00
300_L303_A1.3181.00
149_P196_S1.3161.00
192_I195_E1.3151.00
191_E225_L1.2991.00
220_K224_A1.2971.00
95_E99_R1.2951.00
133_K161_D1.2861.00
27_H213_Q1.2851.00
183_Y187_V1.2821.00
141_C145_G1.2821.00
127_D130_Q1.2801.00
90_K128_A1.2691.00
59_N62_S1.2631.00
128_A131_E1.2601.00
171_Q175_S1.2571.00
38_H42_H1.2551.00
272_I291_A1.2541.00
241_T244_E1.2361.00
172_R176_N1.2261.00
241_T245_G1.2241.00
170_S217_D1.2221.00
26_L260_L1.2181.00
22_A216_I1.2181.00
22_A220_K1.2171.00
204_A218_I1.1981.00
289_Y317_L1.1921.00
211_T214_D1.1811.00
312_K315_S1.1751.00
214_D217_D1.1731.00
171_Q183_Y1.1711.00
251_P283_L1.1691.00
153_K156_K1.1671.00
206_I257_V1.1651.00
131_E134_E1.1601.00
33_I256_K1.1481.00
187_V221_S1.1471.00
44_R48_Y1.1461.00
24_S28_C1.1401.00
153_K196_S1.1331.00
206_I254_C1.1321.00
126_V158_A1.1311.00
188_N191_E1.1201.00
29_P256_K1.1201.00
86_R115_R1.1171.00
171_Q221_S1.1081.00
94_L128_A1.1071.00
91_E95_E1.0991.00
64_L86_R1.0941.00
118_S121_E1.0931.00
142_A145_G1.0911.00
219_A264_I1.0841.00
17_V22_A1.0751.00
280_L286_L1.0721.00
16_E223_K1.0571.00
165_H186_R1.0531.00
43_I46_H1.0521.00
309_E312_K1.0451.00
154_R158_A1.0431.00
121_E124_Q1.0421.00
190_V200_P1.0361.00
167_L183_Y1.0351.00
124_Q127_D1.0341.00
39_G43_I1.0211.00
26_L257_V1.0191.00
139_K143_C1.0171.00
216_I220_K1.0111.00
172_R211_T1.0031.00
288_L314_L1.0011.00
90_K94_L0.9971.00
88_V93_L0.9961.00
183_Y221_S0.9951.00
22_A260_L0.9911.00
140_I145_G0.9901.00
217_D220_K0.9881.00
258_L287_G0.9851.00
26_L212_K0.9821.00
165_H189_T0.9781.00
128_A132_I0.9681.00
97_A132_I0.9630.99
221_S224_A0.9590.99
63_G74_Q0.9590.99
36_L256_K0.9530.99
96_G99_R0.9520.99
16_E266_P0.9480.99
315_S321_V0.9390.99
39_G42_H0.9390.99
50_K319_F0.9350.99
315_S320_E0.9330.99
60_A93_L0.9320.99
120_R123_D0.9280.99
122_V155_L0.9220.99
60_A112_A0.9180.99
68_N75_S0.9140.99
41_F319_F0.9130.99
62_S112_A0.9100.99
250_N253_Y0.9070.99
44_R319_F0.9060.99
212_K257_V0.9040.99
29_P32_D0.9020.99
140_I143_C0.8820.99
99_R103_L0.8800.99
255_L290_A0.8760.99
170_S209_K0.8720.99
28_C36_L0.8670.99
63_G82_I0.8640.99
222_L230_I0.8620.99
101_H137_G0.8590.99
139_K142_A0.8560.99
33_I37_M0.8530.99
194_K200_P0.8530.99
242_P245_G0.8530.99
54_L314_L0.8520.99
144_L163_Y0.8440.99
285_P313_M0.8420.99
282_T285_P0.8410.99
187_V191_E0.8350.99
129_V160_V0.8310.98
130_Q158_A0.8300.98
192_I196_S0.8260.98
73_S238_I0.8250.98
101_H136_Y0.8240.98
94_L134_E0.8220.98
191_E224_A0.8200.98
173_N209_K0.8140.98
241_T246_V0.8130.98
15_A21_E0.8090.98
152_A198_L0.8080.98
40_A289_Y0.8050.98
58_M108_Y0.8040.98
163_Y193_A0.8010.98
314_L321_V0.8010.98
94_L97_A0.8000.98
236_H302_T0.8000.98
174_H186_R0.7980.98
98_K133_K0.7980.98
51_K322_E0.7980.98
35_L38_H0.7970.98
60_A110_I0.7960.98
12_L39_G0.7930.98
239_D244_E0.7910.98
170_S173_N0.7900.98
94_L133_K0.7870.98
314_L319_F0.7870.98
123_D127_D0.7860.98
68_N180_S0.7860.98
222_L265_N0.7810.98
141_C144_L0.7800.98
141_C147_L0.7800.98
96_G108_Y0.7750.98
119_N123_D0.7670.97
85_Y92_T0.7660.97
194_K226_D0.7660.97
251_P280_L0.7650.97
64_L84_S0.7620.97
47_F269_E0.7610.97
37_M259_A0.7610.97
208_M246_V0.7600.97
56_M105_I0.7590.97
237_A245_G0.7580.97
232_V261_F0.7570.97
94_L132_I0.7540.97
75_S242_P0.7530.97
42_H45_K0.7510.97
109_C273_S0.7460.97
220_K223_K0.7400.97
243_L246_V0.7400.97
216_I264_I0.7380.97
98_K101_H0.7380.97
123_D126_V0.7360.97
67_E114_G0.7360.97
219_A222_L0.7340.97
173_N208_M0.7330.97
81_P240_G0.7320.97
110_I128_A0.7280.97
37_M255_L0.7270.96
125_V144_L0.7260.96
67_E180_S0.7260.96
28_C38_H0.7260.96
172_R175_S0.7240.96
308_T311_H0.7230.96
52_V319_F0.7150.96
44_R52_V0.7150.96
258_L291_A0.7100.96
127_D131_E0.7090.96
98_K136_Y0.7080.96
160_V198_L0.7070.96
177_I243_L0.7040.96
97_A136_Y0.7020.96
184_D221_S0.7000.96
62_S85_Y0.6990.96
47_F266_P0.6980.96
56_M100_A0.6970.96
26_L216_I0.6940.95
151_Q154_R0.6940.95
23_L212_K0.6940.95
167_L221_S0.6930.95
255_L286_L0.6900.95
88_V96_G0.6870.95
216_I260_L0.6820.95
152_A189_T0.6820.95
120_R124_Q0.6810.95
16_E227_A0.6790.95
54_L321_V0.6790.95
279_N283_L0.6790.95
256_K260_L0.6790.95
176_N209_K0.6770.95
219_A265_N0.6760.95
101_H106_G0.6710.95
28_C256_K0.6690.94
199_S269_E0.6690.94
234_F254_C0.6680.94
91_E94_L0.6670.94
148_K189_T0.6670.94
169_T173_N0.6670.94
143_C147_L0.6670.94
269_E292_N0.6660.94
273_S295_F0.6660.94
252_L283_L0.6650.94
237_A244_E0.6620.94
78_S242_P0.6610.94
17_V264_I0.6610.94
47_F267_S0.6600.94
169_T214_D0.6590.94
139_K145_G0.6580.94
146_L186_R0.6570.94
78_S116_G0.6560.94
212_K215_V0.6540.94
37_M41_F0.6500.94
76_A83_E0.6490.93
190_V202_S0.6450.93
52_V288_L0.6370.93
222_L228_D0.6350.93
148_K185_D0.6340.93
242_P246_V0.6330.93
24_S27_H0.6320.93
53_K322_E0.6320.93
20_E24_S0.6320.93
129_V140_I0.6310.93
295_F300_L0.6300.92
54_L307_E0.6270.92
240_G246_V0.6260.92
20_E213_Q0.6250.92
12_L46_H0.6240.92
119_N122_V0.6220.92
314_L317_L0.6220.92
193_A198_L0.6210.92
124_Q128_A0.6200.92
148_K192_I0.6180.92
287_G291_A0.6150.92
34_L37_M0.6150.92
190_V194_K0.6150.92
206_I215_V0.6140.92
34_L286_L0.6130.91
249_L254_C0.6120.91
174_H178_T0.6120.91
130_Q134_E0.6100.91
55_N233_N0.6100.91
35_L39_G0.6090.91
21_E24_S0.6080.91
296_V303_A0.6060.91
143_C146_L0.6050.91
130_Q157_D0.6040.91
274_G291_A0.6030.91
252_L256_K0.6020.91
265_N268_K0.6020.91
71_Y186_R0.6000.91
283_L286_L0.5980.90
70_G78_S0.5980.90
201_C233_N0.5940.90
281_R309_E0.5940.90
22_A219_A0.5930.90
68_N76_A0.5930.90
206_I249_L0.5910.90
109_C164_N0.5910.90
70_G176_N0.5890.90
122_V142_A0.5880.90
122_V154_R0.5870.90
130_Q133_K0.5870.90
295_F303_A0.5860.90
253_Y257_V0.5860.90
34_L38_H0.5860.90
115_R146_L0.5850.89
144_L147_L0.5830.89
144_L155_L0.5820.89
251_P286_L0.5820.89
281_R313_M0.5810.89
55_N166_N0.5790.89
48_Y292_N0.5790.89
122_V151_Q0.5760.89
250_N254_C0.5740.89
293_S298_D0.5730.88
59_N275_G0.5710.88
59_N273_S0.5700.88
141_C162_R0.5690.88
305_Q309_E0.5690.88
205_I208_M0.5670.88
152_A163_Y0.5660.88
231_P234_F0.5650.88
74_Q109_C0.5650.88
227_A266_P0.5630.88
222_L268_K0.5590.87
213_Q217_D0.5590.87
33_I252_L0.5590.87
262_R292_N0.5580.87
59_N205_I0.5570.87
50_K314_L0.5570.87
187_V225_L0.5560.87
202_S227_A0.5560.87
140_I144_L0.5560.87
38_H41_F0.5550.87
193_A196_S0.5550.87
147_L151_Q0.5530.87
141_C146_L0.5530.87
313_M316_D0.5530.87
261_F265_N0.5530.87
274_G284_Q0.5510.86
140_I160_V0.5510.86
103_L308_T0.5500.86
52_V314_L0.5490.86
142_A146_L0.5480.86
167_L225_L0.5470.86
258_L270_I0.5460.86
225_L229_S0.5450.86
251_P282_T0.5450.86
48_Y51_K0.5440.86
295_F299_Y0.5440.86
63_G73_S0.5430.86
60_A144_L0.5430.86
73_S299_Y0.5420.86
152_A193_A0.5420.86
142_A160_V0.5400.85
95_E102_D0.5350.85
285_P317_L0.5330.85
277_E305_Q0.5330.85
238_I241_T0.5300.84
194_K228_D0.5290.84
107_T141_C0.5270.84
276_R287_G0.5270.84
62_S113_S0.5270.84
94_L136_Y0.5250.84
112_A125_V0.5250.84
58_M297_G0.5250.84
19_D22_A0.5240.84
173_N177_I0.5230.84
28_C32_D0.5230.84
226_D268_K0.5210.83
172_R213_Q0.5210.83
201_C273_S0.5190.83
296_V299_Y0.5180.83
96_G100_A0.5170.83
257_V260_L0.5160.83
59_N111_V0.5150.83
53_K201_C0.5140.82
212_K260_L0.5120.82
14_G21_E0.5110.82
205_I275_G0.5110.82
293_S296_V0.5100.82
201_C275_G0.5090.82
129_V133_K0.5080.82
36_L259_A0.5060.82
199_S228_D0.5060.82
282_T286_L0.5020.81
210_E215_V0.5000.81
133_K136_Y0.5000.81
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1r30A 2 0.9194 100 0.407 Contact Map
3t7vA 1 0.9552 100 0.421 Contact Map
4r33A 2 0.9672 100 0.441 Contact Map
3iixA 1 0.9701 100 0.449 Contact Map
4wcxA 1 0.9791 100 0.483 Contact Map
4rtbA 2 0.9582 100 0.506 Contact Map
4u0oB 1 0.7642 100 0.665 Contact Map
4jc0A 1 0.9075 99.8 0.77 Contact Map
2qgqA 1 0.7284 99.8 0.771 Contact Map
1oltA 1 0.9254 99.8 0.775 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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