GREMLIN Database
BIOI - Biotin biosynthesis cytochrome P450
UniProt: P53554 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 395 (357)
Sequences: 44279 (32758)
Seq/√Len: 1733.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
322_N325_V3.9921.00
322_N327_D3.2641.00
301_R304_E3.2131.00
200_K222_E2.9911.00
243_L355_A2.9781.00
260_K329_T2.9641.00
292_D302_Q2.9101.00
247_S359_I2.8551.00
117_H361_T2.8321.00
44_G47_E2.8061.00
259_L263_E2.7611.00
247_S275_C2.7601.00
199_R206_D2.6911.00
269_G356_Q2.6591.00
294_D299_T2.6491.00
292_D299_T2.4771.00
171_F174_S2.4331.00
294_D297_G2.4331.00
289_A300_I2.4271.00
258_L262_R2.4011.00
257_Q267_L2.3541.00
250_C279_E2.3521.00
124_Q366_M2.2741.00
197_Q222_E2.2531.00
315_R324_D2.2421.00
124_Q365_R2.2011.00
261_L271_A2.1891.00
40_W304_E2.1101.00
42_V51_I2.0921.00
262_R367_P2.0631.00
193_K226_A2.0351.00
254_H257_Q2.0271.00
31_K38_P2.0031.00
250_C278_Y1.9561.00
139_L358_A1.9301.00
198_K202_H1.9081.00
47_E296_C1.8881.00
193_K222_E1.8771.00
113_I361_T1.8751.00
29_I42_V1.8741.00
136_A244_I1.8401.00
260_K267_L1.8341.00
258_L367_P1.8341.00
21_D24_R1.8021.00
111_Y146_N1.7971.00
48_T313_A1.7821.00
259_L367_P1.7821.00
193_K197_Q1.7641.00
275_C326_F1.7491.00
197_Q201_R1.7491.00
276_L337_S1.7451.00
244_I358_A1.7181.00
59_V293_I1.7101.00
259_L262_R1.7061.00
247_S251_L1.6991.00
221_T224_E1.6861.00
183_I187_Q1.6831.00
273_E352_R1.6761.00
344_V348_S1.6751.00
92_L228_T1.6741.00
194_E198_K1.6551.00
28_P44_G1.6541.00
272_V355_A1.6401.00
33_S38_P1.5761.00
160_E164_S1.5681.00
292_D301_R1.5651.00
114_E146_N1.5491.00
136_A248_V1.5391.00
362_L369_L1.5311.00
206_D209_S1.5291.00
249_L253_Q1.5251.00
247_S271_A1.5061.00
56_R291_E1.5031.00
49_A53_K1.5001.00
19_F22_T1.4911.00
121_D365_R1.4881.00
261_L363_L1.4871.00
243_L281_P1.4731.00
270_T329_T1.4541.00
311_G315_R1.4491.00
58_K291_E1.4451.00
257_Q260_K1.4441.00
314_N337_S1.4441.00
111_Y114_E1.4391.00
317_P324_D1.4301.00
250_C254_H1.4191.00
258_L363_L1.4141.00
115_T146_N1.4061.00
115_T118_H1.4041.00
267_L329_T1.4001.00
190_A194_E1.3981.00
186_V190_A1.3951.00
48_T308_L1.3941.00
157_Q161_W1.3931.00
252_L258_L1.3721.00
87_P90_R1.3711.00
109_Q356_Q1.3691.00
113_I117_H1.3661.00
248_V362_L1.3561.00
361_T364_Q1.3391.00
46_E296_C1.3351.00
261_L268_I1.3351.00
257_Q271_A1.3341.00
209_S213_K1.3271.00
317_P323_P1.3241.00
86_Q90_R1.3181.00
128_K372_A1.3161.00
42_V47_E1.3061.00
278_Y315_R1.2961.00
268_I363_L1.2951.00
30_Y41_Y1.2901.00
197_Q200_K1.2851.00
120_L366_M1.2821.00
161_W187_Q1.2741.00
19_F23_L1.2731.00
22_T25_A1.2701.00
291_E302_Q1.2671.00
271_A326_F1.2651.00
57_F291_E1.2641.00
126_K134_D1.2571.00
116_V139_L1.2541.00
198_K201_R1.2531.00
157_Q187_Q1.2521.00
203_P213_K1.2491.00
21_D324_D1.2351.00
273_E335_H1.2331.00
164_S180_E1.2271.00
54_D57_F1.2191.00
268_I360_N1.2081.00
157_Q160_E1.2041.00
119_L139_L1.1951.00
104_T107_S1.1941.00
28_P318_S1.1891.00
43_T309_L1.1771.00
117_H121_D1.1711.00
293_I299_T1.1681.00
91_R224_E1.1661.00
262_R363_L1.1631.00
179_T183_I1.1611.00
271_A275_C1.1531.00
265_P364_Q1.1391.00
260_K263_E1.1381.00
112_I143_V1.1371.00
40_W300_I1.1341.00
115_T142_F1.1341.00
251_L257_Q1.1261.00
314_N335_H1.1201.00
120_L361_T1.1141.00
106_E109_Q1.1121.00
199_R209_S1.1121.00
42_V48_T1.1121.00
144_I236_G1.1091.00
105_T353_L1.1091.00
50_A54_D1.1021.00
109_Q113_I1.0981.00
257_Q328_I1.0981.00
116_V357_I1.0961.00
58_K293_I1.0931.00
269_G273_E1.0931.00
87_P91_R1.0871.00
22_T26_V1.0771.00
156_E160_E1.0691.00
288_V303_G1.0691.00
114_E118_H1.0631.00
321_T327_D1.0571.00
122_Q126_K1.0551.00
46_E318_S1.0551.00
112_I353_L1.0531.00
215_R222_E1.0521.00
121_D124_Q1.0511.00
116_V120_L1.0461.00
189_M226_A1.0431.00
317_P321_T1.0411.00
88_D91_R1.0391.00
289_A301_R1.0371.00
27_H30_Y1.0341.00
319_I334_P1.0301.00
112_I350_L1.0231.00
195_L198_K1.0171.00
45_Y319_I1.0141.00
79_Q88_D1.0121.00
351_A355_A1.0111.00
367_P370_N1.0081.00
24_R317_P1.0051.00
55_A59_V1.0041.00
84_Q88_D1.0041.00
261_L267_L1.0011.00
257_Q329_T0.9991.00
109_Q357_I0.9981.00
252_L371_L0.9981.00
44_G318_S0.9951.00
251_L261_L0.9871.00
29_I47_E0.9811.00
47_E50_A0.9791.00
46_E50_A0.9771.00
118_H142_F0.9761.00
141_S237_H0.9741.00
175_R178_L0.9701.00
289_A304_E0.9701.00
223_E227_S0.9651.00
271_A328_I0.9611.00
39_G307_Y0.9601.00
176_K180_E0.9581.00
113_I360_N0.9571.00
255_P259_L0.9561.00
289_A292_D0.9531.00
100_F346_L0.9481.00
90_R94_T0.9461.00
215_R219_K0.9461.00
130_E134_D0.9451.00
246_N279_E0.9421.00
113_I357_I0.9411.00
348_S352_R0.9361.00
96_A346_L0.9311.00
244_I355_A0.9281.00
118_H122_Q0.9251.00
267_L328_I0.9211.00
145_A152_E0.9201.00
374_F377_R0.9191.00
205_Q209_S0.9171.00
252_L369_L0.9151.00
251_L258_L0.9151.00
164_S184_M0.9071.00
101_T104_T0.9051.00
117_H365_R0.9041.00
250_C275_C0.9041.00
331_S334_P0.9031.00
118_H121_D0.8961.00
131_V248_V0.8961.00
116_V361_T0.8961.00
146_N151_P0.8951.00
150_V198_K0.8951.00
250_C253_Q0.8931.00
123_V135_F0.8921.00
254_H325_V0.8891.00
50_A293_I0.8881.00
187_Q190_A0.8871.00
115_T151_P0.8861.00
189_M193_K0.8851.00
135_F358_A0.8841.00
256_E260_K0.8821.00
139_L143_V0.8791.00
102_P106_E0.8751.00
288_V305_Q0.8731.00
256_E259_L0.8651.00
182_N186_V0.8631.00
55_A293_I0.8591.00
122_Q125_G0.8571.00
265_P360_N0.8571.00
266_D270_T0.8531.00
220_L224_E0.8501.00
360_N364_Q0.8461.00
23_L30_Y0.8441.00
222_E226_A0.8431.00
130_E133_S0.8411.00
285_T310_L0.8331.00
60_R63_L0.8331.00
160_E163_A0.8331.00
200_K203_P0.8301.00
180_E184_M0.8291.00
267_L270_T0.8291.00
289_A303_G0.8281.00
189_M223_E0.8251.00
194_E197_Q0.8251.00
58_K61_T0.8241.00
195_L199_R0.8171.00
192_F229_C0.8151.00
268_I356_Q0.8131.00
248_V363_L0.8121.00
247_S326_F0.8101.00
80_M231_L0.8091.00
163_A167_Q0.8091.00
199_R203_P0.8061.00
251_L363_L0.8061.00
46_E49_A0.8031.00
143_V354_E0.7991.00
243_L275_C0.7991.00
50_A296_C0.7971.00
55_A58_K0.7961.00
140_A240_T0.7931.00
238_E242_N0.7921.00
158_L161_W0.7921.00
136_A245_S0.7911.00
29_I298_V0.7901.00
352_R356_Q0.7881.00
210_M213_K0.7881.00
299_T302_Q0.7861.00
88_D224_E0.7841.00
255_P258_L0.7831.00
110_P114_E0.7811.00
122_Q138_P0.7801.00
246_N378_Y0.7791.00
178_L182_N0.7791.00
248_V359_I0.7781.00
185_A189_M0.7771.00
31_K40_W0.7761.00
34_F39_G0.7761.00
51_I295_I0.7751.00
196_I200_K0.7721.00
274_E277_R0.7721.00
247_S272_V0.7701.00
51_I308_L0.7661.00
121_D125_G0.7641.00
208_I229_C0.7611.00
161_W164_S0.7571.00
180_E183_I0.7541.00
44_G296_C0.7531.00
164_S167_Q0.7531.00
264_N267_L0.7521.00
111_Y115_T0.7501.00
350_L354_E0.7501.00
269_G352_R0.7491.00
193_K223_E0.7411.00
249_L371_L0.7411.00
268_I359_I0.7391.00
187_Q191_Y0.7381.00
18_S21_D0.7361.00
160_E187_Q0.7341.00
200_K215_R0.7331.00
280_S314_N0.7311.00
149_G206_D0.7281.00
204_Q209_S0.7261.00
324_D327_D0.7261.00
86_Q342_H0.7241.00
48_T336_L0.7241.00
236_G239_T0.7231.00
176_K179_T0.7231.00
189_M230_I0.7211.00
310_L336_L0.7191.00
159_K163_A0.7161.00
316_D319_I0.7141.00
175_R179_T0.7131.00
186_V189_M0.7121.00
154_D191_Y0.7121.00
32_G39_G0.7111.00
267_L271_A0.7111.00
164_S187_Q0.7091.00
88_D92_L0.7081.00
157_Q194_E0.7051.00
89_H93_R0.7021.00
190_A193_K0.7021.00
103_R107_S0.7011.00
114_E151_P0.7001.00
41_Y309_L0.6961.00
38_P304_E0.6941.00
156_E159_K0.6901.00
184_M187_Q0.6891.00
322_N330_R0.6891.00
270_T328_I0.6871.00
120_L365_R0.6861.00
164_S168_T0.6861.00
278_Y324_D0.6831.00
300_I306_V0.6801.00
166_I169_I0.6801.00
199_R202_H0.6791.00
240_T354_E0.6781.00
189_M227_S0.6771.00
52_L308_L0.6771.00
84_Q92_L0.6771.00
28_P316_D0.6771.00
21_D312_A0.6761.00
295_I300_I0.6751.00
357_I361_T0.6741.00
107_S205_Q0.6741.00
257_Q327_D0.6721.00
162_A166_I0.6701.00
103_R106_E0.6701.00
122_Q135_F0.6691.00
235_A239_T0.6671.00
96_A232_L0.6661.00
276_L335_H0.6621.00
80_M84_Q0.6591.00
109_Q353_L0.6591.00
33_S36_K0.6591.00
296_C299_T0.6591.00
356_Q360_N0.6581.00
205_Q213_K0.6571.00
361_T365_R0.6571.00
260_K264_N0.6571.00
253_Q371_L0.6571.00
39_G305_Q0.6571.00
32_G41_Y0.6491.00
140_A354_E0.6481.00
292_D304_E0.6471.00
58_K290_S0.6461.00
105_T350_L0.6451.00
31_K304_E0.6441.00
102_P349_S0.6431.00
50_A55_A0.6421.00
309_L312_A0.6381.00
53_K340_H0.6371.00
25_A318_S0.6371.00
140_A241_V0.6371.00
37_Y305_Q0.6361.00
280_S283_Q0.6361.00
61_T290_S0.6351.00
243_L276_L0.6351.00
253_Q375_E0.6321.00
131_V136_A0.6301.00
59_V62_P0.6301.00
268_I271_A0.6281.00
138_P142_F0.6281.00
272_V356_Q0.6271.00
50_A295_I0.6271.00
286_A307_Y0.6271.00
239_T282_T0.6261.00
251_L254_H0.6241.00
81_M231_L0.6231.00
251_L271_A0.6211.00
116_V358_A0.6211.00
74_S79_Q0.6211.00
327_D330_R0.6191.00
45_Y316_D0.6181.00
118_H151_P0.6171.00
155_R159_K0.6151.00
28_P31_K0.6131.00
289_A302_Q0.6131.00
27_H298_V0.6121.00
248_V369_L0.6121.00
110_P113_I0.6121.00
339_G344_V0.6121.00
60_R290_S0.6101.00
45_Y49_A0.6101.00
144_I147_I0.6091.00
104_T108_Y0.6091.00
89_H343_H0.6091.00
51_I57_F0.6071.00
61_T84_Q0.6071.00
96_A228_T0.6071.00
28_P296_C0.6061.00
40_W298_V0.6051.00
321_T325_V0.6051.00
141_S233_A0.6031.00
320_F333_N0.6021.00
290_S302_Q0.6021.00
93_R97_S0.6011.00
178_L181_G0.5981.00
177_A181_G0.5981.00
56_R59_V0.5971.00
270_T327_D0.5961.00
340_H344_V0.5951.00
250_C326_F0.5941.00
154_D157_Q0.5931.00
320_F323_P0.5921.00
48_T52_L0.5911.00
255_P367_P0.5911.00
196_I212_L0.5911.00
99_A210_M0.5901.00
21_D25_A0.5891.00
158_L162_A0.5871.00
242_N246_N0.5871.00
142_F155_R0.5841.00
159_K162_A0.5831.00
119_L123_V0.5801.00
112_I357_I0.5801.00
95_L214_G0.5801.00
40_W301_R0.5791.00
289_A293_I0.5781.00
134_D138_P0.5761.00
100_F232_L0.5731.00
123_V129_M0.5731.00
183_I186_V0.5701.00
240_T355_A0.5691.00
131_V135_F0.5681.00
161_W165_L0.5681.00
23_L32_G0.5671.00
373_D376_W0.5671.00
147_I232_L0.5661.00
228_T231_L0.5651.00
50_A294_D0.5641.00
119_L358_A0.5641.00
199_R204_Q0.5621.00
256_E325_V0.5611.00
167_Q170_D0.5611.00
162_A237_H0.5611.00
104_T210_M0.5601.00
49_A333_N0.5601.00
182_N185_A0.5591.00
247_S355_A0.5591.00
68_T71_Q0.5561.00
205_Q210_M0.5551.00
272_V352_R0.5551.00
161_W166_I0.5531.00
140_A144_I0.5531.00
181_G185_A0.5501.00
179_T182_N0.5481.00
30_Y43_T0.5481.00
166_I170_D0.5471.00
250_C257_Q0.5461.00
41_Y307_Y0.5381.00
114_E117_H0.5371.00
119_L362_L0.5341.00
351_A354_E0.5331.00
165_L168_T0.5311.00
243_L272_V0.5291.00
155_R160_E0.5291.00
116_V119_L0.5291.00
207_M211_L0.5291.00
112_I354_E0.5281.00
28_P43_T0.5251.00
136_A355_A0.5251.00
120_L123_V0.5241.00
91_R218_D0.5231.00
52_L310_L0.5221.00
46_E319_I0.5211.00
136_A140_A0.5211.00
333_N340_H0.5211.00
255_P370_N0.5201.00
146_N155_R0.5161.00
53_K56_R0.5151.00
81_M96_A0.5141.00
71_Q74_S0.5141.00
375_E378_Y0.5131.00
47_E295_I0.5121.00
211_L225_A0.5121.00
43_T316_D0.5111.00
196_I208_I0.5101.00
44_G48_T0.5091.00
122_Q142_F0.5091.00
181_G184_M0.5091.00
276_L281_P0.5081.00
224_E228_T0.5081.00
206_D210_M0.5071.00
44_G313_A0.5071.00
120_L124_Q0.5041.00
268_I272_V0.5031.00
192_F195_L0.5031.00
81_M228_T0.5031.00
137_F241_V0.5031.00
363_L369_L0.5021.00
135_F138_P0.5011.00
298_V301_R0.5011.00
82_L231_L0.5011.00
283_Q311_G0.5011.00
79_Q223_E0.5001.00
289_A306_V0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3ejbB 1 0.9722 100 0.174 Contact Map
2uuqA 2 0.9722 100 0.185 Contact Map
2z3tA 1 0.9544 100 0.189 Contact Map
4tpnA 1 0.9468 100 0.19 Contact Map
3nc3A 1 0.9392 100 0.194 Contact Map
3bujA 1 0.9772 100 0.195 Contact Map
4egoA 3 0.9772 100 0.198 Contact Map
2xkrA 1 0.9646 100 0.198 Contact Map
1z8oA 1 0.9747 100 0.199 Contact Map
3r9bA 3 0.9696 100 0.204 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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