GREMLIN Database
PANB - 3-methyl-2-oxobutanoate hydroxymethyltransferase
UniProt: P52996 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 277 (261)
Sequences: 3115 (1877)
Seq/√Len: 116.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
16_P254_R3.4631.00
86_M100_A3.0671.00
189_A193_E2.8711.00
235_R238_E2.8641.00
123_I170_C2.8431.00
67_T81_T2.7441.00
233_Y250_D2.6401.00
189_A207_K2.5921.00
19_M212_V2.5881.00
60_V98_N2.5811.00
171_E196_S2.3581.00
168_I194_T2.2951.00
46_G58_V2.2251.00
11_K209_D2.1651.00
101_A105_E2.1591.00
60_V87_S2.1561.00
171_E195_L2.1551.00
50_L65_H2.1301.00
94_D97_K2.1231.00
231_K250_D2.1041.00
134_V177_M2.0831.00
15_E252_R2.0711.00
18_V248_V2.0211.00
239_T242_T2.0211.00
231_K261_H2.0151.00
36_V248_V1.9561.00
192_A199_V1.9551.00
10_M17_I1.9461.00
90_L97_K1.9341.00
248_V252_R1.8801.00
9_K13_S1.8791.00
161_K165_E1.8751.00
124_R128_L1.8301.00
117_E165_E1.8181.00
190_K194_T1.8051.00
62_D68_K1.7531.00
71_K79_I1.7391.00
234_T239_T1.7311.00
60_V102_I1.7191.00
249_Q253_H1.7061.00
185_A262_S1.7031.00
134_V176_M1.6881.00
162_K166_D1.6711.00
242_T246_G1.6671.00
31_A39_I1.6551.00
83_M98_N1.6481.00
32_E77_T1.6191.00
31_A244_I1.6151.00
67_T108_A1.6101.00
156_D159_S1.5971.00
81_T102_I1.5901.00
135_S170_C1.5771.00
258_E261_H1.5501.00
168_I171_E1.5411.00
54_S57_G1.5291.00
67_T106_S1.5271.00
175_A197_I1.5131.00
50_L69_A1.5111.00
39_I74_A1.5091.00
102_I106_S1.4901.00
7_F38_M1.4841.00
38_M212_V1.4781.00
226_T232_Q1.4741.00
153_Q159_S1.4681.00
164_I190_K1.4621.00
250_D255_A1.4451.00
27_A215_Y1.4421.00
206_V262_S1.4341.00
232_Q235_R1.4311.00
135_S175_A1.4241.00
95_T122_S1.4231.00
10_M15_E1.4011.00
238_E242_T1.3971.00
33_Q241_E1.3961.00
83_M99_A1.3401.00
110_A176_M1.3371.00
171_E197_I1.3311.00
38_M134_V1.3301.00
61_A101_A1.3291.00
178_L195_L1.3271.00
81_T106_S1.3081.00
19_M38_M1.2991.00
137_L163_L1.2911.00
125_A129_G1.2901.00
170_C175_A1.2861.00
90_L94_D1.2800.99
168_I195_L1.2710.99
92_K122_S1.2700.99
135_S179_V1.2630.99
137_L180_L1.2570.99
219_I240_I1.2550.99
140_T147_L1.2440.99
64_I102_I1.2350.99
109_D145_G1.2320.99
20_L218_I1.2240.99
178_L199_V1.2190.99
117_E166_D1.2140.99
124_R172_E1.2120.99
157_E161_K1.2060.99
80_V179_V1.1990.99
8_L12_E1.1910.99
31_A215_Y1.1770.99
32_E76_N1.1690.99
17_I210_G1.1550.99
68_K72_R1.1480.99
46_G52_L1.1430.99
90_L93_E1.1310.99
121_E124_R1.1240.99
178_L197_I1.1220.99
81_T108_A1.1170.99
74_A79_I1.1150.99
18_V252_R1.1100.99
52_L57_G1.1080.98
199_V208_A1.1080.98
185_A207_K1.1040.98
52_L72_R1.1010.98
88_Y113_L1.1000.98
80_V134_V1.0990.98
96_L125_A1.0990.98
118_G162_K1.0980.98
215_Y219_I1.0940.98
180_L183_V1.0870.98
127_T175_A1.0810.98
32_E75_P1.0680.98
192_A208_A1.0640.98
219_I236_I1.0640.98
245_S249_Q1.0600.98
154_G191_I1.0500.98
241_E245_S1.0500.98
92_K125_A1.0500.98
94_D98_N1.0450.98
6_D9_K1.0330.98
80_V110_A1.0260.98
258_E262_S1.0160.97
38_M80_V1.0160.97
5_L151_K1.0150.97
45_L66_H1.0080.97
91_S94_D1.0020.97
29_K224_E1.0010.97
182_C263_F0.9990.97
18_V36_V0.9930.97
52_L62_D0.9920.97
120_F166_D0.9880.97
185_A206_V0.9870.97
139_L182_C0.9850.97
188_T208_A0.9830.97
96_L126_L0.9740.97
160_A187_L0.9740.97
118_G128_L0.9680.96
165_E169_K0.9630.96
49_V216_H0.9620.96
222_G225_R0.9590.96
83_M87_S0.9480.96
46_G62_D0.9470.96
62_D65_H0.9390.96
26_P49_V0.9370.96
30_L244_I0.9330.96
120_F169_K0.9260.95
61_A64_I0.9230.95
246_G249_Q0.9210.95
166_D169_K0.9160.95
93_E97_K0.9140.95
17_I37_D0.9120.95
246_G250_D0.9090.95
26_P221_H0.9070.95
71_K74_A0.9060.95
119_V122_S0.9030.95
192_A209_D0.9010.95
190_K193_E0.9000.95
147_L182_C0.8950.95
57_G104_Q0.8940.94
206_V254_R0.8910.94
180_L191_I0.8910.94
88_Y95_T0.8830.94
60_V63_M0.8820.94
20_L31_A0.8780.94
159_S162_K0.8730.94
96_L129_G0.8730.94
144_V149_G0.8710.94
39_I79_I0.8680.94
78_F110_A0.8670.94
242_T245_S0.8640.93
20_L36_V0.8590.93
64_I106_S0.8590.93
233_Y243_A0.8570.93
127_T176_M0.8560.93
164_I168_I0.8550.93
179_V197_I0.8540.93
103_V132_P0.8490.93
45_L58_V0.8480.93
71_K107_G0.8450.93
37_D252_R0.8440.93
236_I240_I0.8400.92
79_I109_D0.8400.92
65_H69_A0.8400.92
237_D241_E0.8400.92
89_H104_Q0.8380.92
161_K190_K0.8370.92
50_L62_D0.8320.92
154_G163_L0.8260.92
117_E162_K0.8230.92
26_P216_H0.8230.92
117_E169_K0.8220.92
213_L229_F0.8220.92
184_P204_A0.8220.92
164_I194_T0.8200.92
29_K33_Q0.8160.91
116_G120_F0.8050.91
87_S104_Q0.8030.91
170_C178_L0.8020.91
110_A134_V0.7960.90
27_A216_H0.7930.90
68_K71_K0.7900.90
92_K121_E0.7870.90
45_L50_L0.7860.90
37_D210_G0.7790.89
96_L100_A0.7790.89
234_T243_A0.7790.89
10_M252_R0.7760.89
18_V37_D0.7750.89
59_T62_D0.7730.89
20_L244_I0.7730.89
152_V228_K0.7680.89
32_E74_A0.7650.88
236_I239_T0.7630.88
17_I38_M0.7630.88
182_C228_K0.7600.88
235_R239_T0.7590.88
250_D253_H0.7590.88
7_F176_M0.7580.88
152_V263_F0.7560.88
30_L237_D0.7530.88
71_K109_D0.7390.87
64_I101_A0.7260.86
229_F261_H0.7240.86
122_S125_A0.7240.86
233_Y255_A0.7240.86
34_A241_E0.7230.86
89_H95_T0.7200.85
64_I105_E0.7190.85
184_P187_L0.7180.85
25_Y29_K0.7160.85
57_G107_G0.7100.84
175_A178_L0.7080.84
169_K172_E0.7050.84
60_V64_I0.7040.84
135_S178_L0.7010.84
147_L153_Q0.6990.84
93_E125_A0.6990.84
215_Y244_I0.6990.84
63_M81_T0.6980.83
27_A240_I0.6970.83
160_A190_K0.6820.82
193_E207_K0.6810.82
20_L215_Y0.6810.82
29_K237_D0.6800.82
45_L65_H0.6790.82
149_G153_Q0.6780.82
71_K106_S0.6770.82
137_L178_L0.6750.81
91_S122_S0.6740.81
8_L196_S0.6720.81
86_M96_L0.6700.81
117_E120_F0.6700.81
88_Y98_N0.6690.81
223_V237_D0.6690.81
227_P230_V0.6670.81
139_L150_Y0.6650.80
168_I172_E0.6640.80
165_E168_I0.6640.80
111_L133_V0.6630.80
216_H221_H0.6580.80
53_D57_G0.6580.80
231_K255_A0.6530.79
46_G59_T0.6520.79
38_M78_F0.6490.79
64_I67_T0.6470.79
24_D27_A0.6450.78
100_A104_Q0.6440.78
92_K95_T0.6430.78
7_F10_M0.6420.78
10_M37_D0.6400.78
122_S126_L0.6380.78
182_C222_G0.6360.77
220_G226_T0.6350.77
256_F263_F0.6350.77
169_K173_A0.6320.77
180_L199_V0.6320.77
115_G166_D0.6270.76
19_M28_A0.6260.76
152_V182_C0.6240.76
191_I194_T0.6240.76
68_K106_S0.6240.76
83_M88_Y0.6210.76
185_A259_Q0.6210.76
113_L126_L0.6210.76
56_V104_Q0.6200.76
220_G232_Q0.6180.75
27_A219_I0.6180.75
200_I210_G0.6170.75
150_Y227_P0.6160.75
259_Q262_S0.6160.75
163_L187_L0.6150.75
47_M227_P0.6130.75
71_K75_P0.6130.75
23_Y49_V0.6130.75
83_M102_I0.6080.74
6_D10_M0.6040.74
127_T146_V0.6040.74
240_I244_I0.6020.73
34_A245_S0.6020.73
146_V176_M0.6020.73
231_K258_E0.6010.73
100_A131_I0.6010.73
4_K176_M0.6010.73
160_A184_P0.5970.73
80_V177_M0.5970.73
9_K12_E0.5910.72
92_K96_L0.5900.72
233_Y236_I0.5870.72
150_Y182_C0.5860.71
179_V195_L0.5860.71
28_A39_I0.5850.71
195_L199_V0.5850.71
230_V263_F0.5820.71
56_V143_S0.5820.71
168_I196_S0.5790.71
4_K8_L0.5790.71
140_T143_S0.5780.70
218_I230_V0.5780.70
137_L154_G0.5750.70
64_I68_K0.5740.70
85_F130_G0.5730.70
49_V69_A0.5720.70
99_A131_I0.5720.70
58_V62_D0.5720.70
28_A74_A0.5720.70
202_I212_V0.5720.70
127_T173_A0.5670.69
120_F173_A0.5650.69
121_E128_L0.5610.68
180_L188_T0.5600.68
222_G226_T0.5580.68
38_M177_M0.5560.67
183_V199_V0.5560.67
123_I175_A0.5560.67
70_V79_I0.5540.67
17_I200_I0.5540.67
103_V126_L0.5530.67
130_G146_V0.5530.67
123_I135_S0.5520.67
25_Y73_G0.5510.67
98_N102_I0.5510.67
56_V144_V0.5490.67
126_L129_G0.5460.66
113_L119_V0.5450.66
10_M13_S0.5440.66
153_Q184_P0.5420.66
22_A31_A0.5400.65
144_V150_Y0.5390.65
116_G122_S0.5350.65
183_V187_L0.5340.64
19_M80_V0.5330.64
215_Y221_H0.5320.64
192_A207_K0.5320.64
199_V209_D0.5300.64
24_D216_H0.5290.64
6_D47_M0.5270.63
54_S107_G0.5250.63
233_Y239_T0.5220.63
96_L122_S0.5220.63
22_A39_I0.5210.63
22_A28_A0.5210.63
152_V184_P0.5200.62
60_V101_A0.5200.62
250_D261_H0.5180.62
33_Q245_S0.5180.62
153_Q263_F0.5170.62
163_L191_I0.5160.62
52_L56_V0.5130.61
22_A41_V0.5120.61
186_E190_K0.5090.61
97_K100_A0.5090.61
4_K146_V0.5060.60
117_E128_L0.5060.60
140_T184_P0.5060.60
39_I244_I0.5050.60
229_F263_F0.5040.60
116_G179_V0.5030.60
23_Y216_H0.5020.60
17_I198_P0.5020.60
78_F176_M0.5000.59
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1o66A 3 0.8989 100 0.246 Contact Map
1oy0A 4 0.8881 100 0.248 Contact Map
1m3uA 4 0.9458 100 0.25 Contact Map
3vavA 4 0.9422 100 0.268 Contact Map
4lsbA 2 0.8989 100 0.534 Contact Map
4mg4A 4 0.8412 100 0.609 Contact Map
2qiwA 2 0.8375 100 0.626 Contact Map
3lyeA 4 0.8881 100 0.627 Contact Map
3eooA 4 0.9134 100 0.631 Contact Map
1xg4A 4 0.8989 100 0.634 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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