GREMLIN Database
MDH - Malate dehydrogenase
UniProt: P49814 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 312 (297)
Sequences: 4891 (2843)
Seq/√Len: 165.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
9_S76_S4.2771.00
212_E216_K2.9781.00
78_I242_V2.9621.00
294_R298_N2.8881.00
80_V242_V2.5891.00
123_L238_L2.4571.00
85_I99_T2.4291.00
9_S73_T2.3831.00
101_E133_A2.3461.00
73_T115_S2.3321.00
27_K243_E2.2741.00
142_K260_E2.2681.00
250_R277_G2.2251.00
213_R220_E2.2041.00
302_E306_N2.2031.00
277_G282_E2.1681.00
270_L297_L2.1611.00
265_Y297_L2.1201.00
110_E113_K2.1091.00
165_L193_A2.0351.00
164_E206_R1.9851.00
209_A213_R1.9591.00
112_V140_F1.9361.00
177_L187_L1.9261.00
274_T281_L1.9011.00
250_R282_E1.8871.00
275_I283_Q1.8841.00
105_R109_Q1.8681.00
119_I245_I1.8621.00
7_K31_D1.8601.00
135_Y269_Y1.8531.00
106_S110_E1.8531.00
265_Y298_N1.8361.00
270_L301_V1.8301.00
257_A274_T1.8191.00
108_T134_V1.8151.00
174_G191_S1.8061.00
7_K33_V1.8031.00
259_L272_V1.7991.00
108_T138_S1.7991.00
298_N302_E1.7791.00
258_Y269_Y1.7741.00
97_V307_V1.7721.00
295_A299_K1.7571.00
140_F144_R1.7191.00
109_Q137_E1.6721.00
109_Q113_K1.6651.00
8_V30_A1.6591.00
291_D294_R1.6121.00
44_T48_A1.6051.00
148_Q238_L1.5721.00
8_V24_I1.5661.00
291_D295_A1.5631.00
104_M134_V1.5601.00
149_S271_G1.5391.00
91_M99_T1.5371.00
185_V293_E1.5341.00
243_E247_K1.5271.00
131_T269_Y1.5101.00
176_V186_P1.5041.00
10_V21_A1.4911.00
13_A34_L1.4901.00
147_G256_I1.4831.00
203_P206_R1.4821.00
296_Q299_K1.4801.00
87_R218_G1.4721.00
102_K105_R1.4711.00
208_D212_E1.4701.00
279_N282_E1.4701.00
20_T238_L1.4681.00
70_Y114_Y1.4601.00
52_L163_E1.4561.00
42_N45_K1.4291.00
132_Y136_K1.4181.00
70_Y113_K1.4141.00
120_I134_V1.3991.00
151_V237_S1.3991.00
52_L159_T1.3981.00
132_Y308_M1.3851.00
105_R137_E1.3801.00
162_A172_V1.3701.00
92_S95_D1.3471.00
81_I122_V1.3441.00
62_A65_T1.3421.00
73_T76_S1.3291.00
299_K302_E1.3221.00
71_E74_A1.3181.00
161_V172_V1.3131.00
111_I140_F1.3091.00
292_Y296_Q1.3081.00
168_S171_D1.3071.00
22_F235_A1.3071.00
252_V275_I1.3041.00
248_D278_G1.2961.00
257_A281_L1.2951.00
183_D303_S1.2931.00
216_K219_G1.2631.00
265_Y301_V1.2631.00
186_P211_V1.2491.00
54_A236_A1.2471.00
41_E45_K1.2441.00
85_I88_K1.2321.00
200_T288_E1.2271.00
151_V253_L1.2241.00
173_T192_Y1.2231.00
212_E215_R1.2221.00
171_D194_G1.2161.00
111_I120_I1.2091.00
135_Y258_Y1.2061.00
295_A298_N1.2031.00
7_K75_G1.2021.00
38_P42_N1.2021.00
204_K207_I1.2001.00
104_M108_T1.1901.00
119_I144_R1.1871.00
78_I121_V1.1841.00
112_V139_G1.1801.00
259_L262_E1.1791.00
164_E209_A1.1761.00
220_E223_N1.1721.00
127_V147_G1.1651.00
20_T242_V1.1641.00
79_V111_I1.1621.00
24_I29_L1.1591.00
220_E224_L1.1581.00
240_E251_R1.1531.00
189_R293_E1.1531.00
41_E69_N1.1501.00
115_S118_S1.1471.00
24_I32_V1.1441.00
77_D116_P1.1371.00
22_F26_Q1.1371.00
106_S109_Q1.1311.00
42_N223_N1.1301.00
133_A137_E1.1271.00
146_I245_I1.1241.00
189_R290_T1.1231.00
305_K309_K1.1221.00
20_T80_V1.1201.00
122_V134_V1.1171.00
255_T274_T1.1111.00
96_L100_N1.0991.00
38_P43_P1.0971.00
93_R125_N1.0941.00
67_T72_D1.0921.00
33_V72_D1.0891.00
306_N309_K1.0821.00
125_N180_H1.0821.00
42_N224_L1.0801.00
143_E279_N1.0750.99
46_G224_L1.0730.99
99_T102_K1.0730.99
298_N301_V1.0730.99
261_G264_G1.0720.99
299_K303_S1.0670.99
260_E266_E1.0670.99
53_E217_G1.0660.99
170_K249_Q1.0450.99
117_D141_P1.0450.99
79_V118_S1.0400.99
135_Y142_K1.0400.99
93_R180_H1.0380.99
77_D117_D1.0330.99
81_I134_V1.0260.99
98_S102_K1.0230.99
20_T235_A1.0220.99
142_K258_Y1.0210.99
15_F231_Y1.0200.99
69_N72_D1.0120.99
192_Y195_G1.0040.99
275_I282_E1.0040.99
262_E285_I1.0000.99
18_A47_K0.9880.99
29_L247_K0.9880.99
183_D300_S0.9850.99
292_Y295_A0.9820.99
158_R173_T0.9780.99
95_D98_S0.9650.99
44_T68_S0.9640.99
216_K223_N0.9620.99
297_L301_V0.9580.99
18_A235_A0.9530.99
91_M95_D0.9500.99
33_V76_S0.9490.99
68_S72_D0.9470.99
289_L297_L0.9450.99
35_V70_Y0.9440.99
187_L289_L0.9420.99
87_R222_V0.9370.99
183_D299_K0.9350.99
196_I200_T0.9330.99
162_A169_V0.9290.99
205_E209_A0.9240.98
108_T120_I0.9230.98
159_T163_E0.9230.98
43_P47_K0.9220.98
121_V241_M0.9220.98
85_I103_I0.9120.98
218_G222_V0.9090.98
275_I285_I0.9080.98
157_F174_G0.9070.98
141_P144_R0.8980.98
104_M130_M0.8920.98
78_I246_L0.8890.98
75_G116_P0.8840.98
243_E249_Q0.8830.98
80_V121_V0.8800.98
74_A114_Y0.8790.98
206_R209_A0.8760.98
21_A34_L0.8760.98
160_F213_R0.8750.98
76_S79_V0.8730.98
29_L239_T0.8720.98
31_D63_K0.8720.98
235_A239_T0.8710.98
132_Y135_Y0.8670.98
71_E114_Y0.8610.98
121_V242_V0.8600.98
129_A307_V0.8580.98
135_Y145_V0.8570.98
131_T147_G0.8550.97
81_I104_M0.8550.97
27_K239_T0.8540.97
127_V131_T0.8540.97
79_V115_S0.8530.97
167_L193_A0.8430.97
87_R219_G0.8430.97
154_T158_R0.8410.97
6_K246_L0.8350.97
200_T203_P0.8300.97
108_T131_T0.8240.97
145_V269_Y0.8240.97
197_P200_T0.8160.97
161_V165_L0.8130.97
238_L242_V0.8100.97
303_S307_V0.8080.96
11_I107_V0.8080.96
160_F163_E0.8080.96
15_F18_A0.8060.96
45_K224_L0.8040.96
260_E267_G0.8020.96
89_P223_N0.8020.96
92_S182_D0.8020.96
24_I30_A0.8000.96
70_Y107_V0.7990.96
87_R96_L0.7980.96
302_E309_K0.7960.96
118_S140_F0.7950.96
75_G117_D0.7940.96
255_T276_V0.7930.96
13_A47_K0.7890.96
104_M122_V0.7880.96
23_L235_A0.7860.96
121_V245_I0.7850.96
272_V284_I0.7810.96
302_E305_K0.7780.96
196_I201_L0.7740.95
177_L185_V0.7730.95
157_F186_P0.7700.95
202_I206_R0.7700.95
20_T24_I0.7680.95
10_V20_T0.7660.95
94_D129_A0.7610.95
188_V214_T0.7600.95
167_L172_V0.7600.95
19_T232_Y0.7600.95
93_R96_L0.7590.95
182_D219_G0.7570.95
277_G280_G0.7510.95
35_V73_T0.7500.95
41_E67_T0.7490.95
108_T112_V0.7490.95
45_K68_S0.7490.95
127_V149_S0.7480.95
20_T82_T0.7460.94
103_I124_T0.7450.94
241_M255_T0.7420.94
76_S115_S0.7420.94
151_V241_M0.7400.94
96_L222_V0.7360.94
112_V138_S0.7340.94
81_I107_V0.7320.94
132_Y145_V0.7290.94
211_V215_R0.7270.94
165_L172_V0.7270.94
143_E280_G0.7230.94
260_E285_I0.7180.93
140_F145_V0.7170.93
41_E68_S0.7170.93
33_V68_S0.7120.93
204_K208_D0.7100.93
87_R91_M0.7100.93
202_I207_I0.7060.93
119_I146_I0.7040.93
268_I305_K0.7030.93
134_V145_V0.7020.93
15_F229_S0.7020.93
185_V300_S0.6960.92
176_V211_V0.6950.92
24_I239_T0.6940.92
23_L26_Q0.6910.92
83_A103_I0.6900.92
221_I233_A0.6890.92
219_G223_N0.6850.92
80_V238_L0.6840.92
70_Y74_A0.6840.92
208_D292_Y0.6770.91
177_L287_L0.6740.91
19_T231_Y0.6740.91
63_K163_E0.6690.91
209_A212_E0.6680.91
157_F191_S0.6670.90
19_T23_L0.6660.90
240_E249_Q0.6650.90
123_L148_Q0.6650.90
268_I301_V0.6640.90
65_T72_D0.6610.90
134_V138_S0.6590.90
24_I242_V0.6560.90
88_K99_T0.6550.90
112_V120_I0.6550.90
102_K106_S0.6530.90
233_A236_A0.6510.89
257_A271_G0.6490.89
94_D307_V0.6480.89
112_V118_S0.6440.89
261_G266_E0.6420.89
241_M253_L0.6410.89
132_Y267_G0.6400.89
47_K235_A0.6380.88
58_Q236_A0.6340.88
289_L298_N0.6330.88
48_A52_L0.6320.88
70_Y110_E0.6320.88
301_V305_K0.6260.88
9_S115_S0.6250.87
282_E285_I0.6250.87
38_P44_T0.6250.87
216_K220_E0.6250.87
9_S33_V0.6230.87
261_G286_E0.6230.87
108_T137_E0.6220.87
265_Y305_K0.6220.87
136_K258_Y0.6210.87
21_A51_M0.6210.87
35_V107_V0.6200.87
265_Y294_R0.6200.87
158_R240_E0.6190.87
173_T251_R0.6190.87
73_T112_V0.6140.86
18_A232_Y0.6140.86
104_M137_E0.6120.86
111_I114_Y0.6110.86
218_G230_A0.6110.86
250_R275_I0.6110.86
101_E137_E0.6100.86
94_D303_S0.6100.86
222_V229_S0.6100.86
23_L239_T0.6100.86
6_K77_D0.6100.86
187_L190_Y0.6100.86
153_D156_R0.6090.86
53_E159_T0.6080.86
93_R153_D0.6070.86
190_Y287_L0.6030.86
162_A167_L0.6010.85
127_V148_Q0.5970.85
218_G229_S0.5950.85
211_V296_Q0.5940.85
25_A51_M0.5940.85
157_F185_V0.5930.85
185_V296_Q0.5910.84
248_D277_G0.5910.84
262_E283_Q0.5900.84
161_V174_G0.5890.84
22_F232_Y0.5890.84
84_G124_T0.5860.84
244_A255_T0.5860.84
110_E114_Y0.5860.84
82_T123_L0.5850.84
142_K269_Y0.5790.83
244_A251_R0.5780.83
281_L284_I0.5780.83
163_E213_R0.5770.83
61_D168_S0.5740.83
185_V211_V0.5660.82
180_H218_G0.5650.82
81_I108_T0.5640.82
91_M96_L0.5620.81
65_T68_S0.5610.81
177_L289_L0.5600.81
185_V289_L0.5580.81
277_G281_L0.5570.81
144_R279_N0.5520.80
10_V32_V0.5520.80
254_P271_G0.5500.80
280_G283_Q0.5500.80
38_P41_E0.5480.80
117_D144_R0.5460.79
46_G96_L0.5460.79
16_T123_L0.5460.79
165_L198_L0.5450.79
193_A201_L0.5450.79
262_E272_V0.5430.79
11_I73_T0.5410.79
100_N130_M0.5400.79
19_T22_F0.5390.79
8_V32_V0.5390.79
191_S198_L0.5350.78
107_V111_I0.5330.78
47_K232_Y0.5300.77
148_Q151_V0.5270.77
148_Q271_G0.5250.77
282_E287_L0.5250.77
265_Y270_L0.5240.77
242_V245_I0.5230.76
270_L304_V0.5230.76
278_G282_E0.5220.76
25_A32_V0.5210.76
179_G230_A0.5200.76
25_A56_P0.5190.76
31_D65_T0.5170.76
78_I119_I0.5170.76
199_E204_K0.5150.75
188_V191_S0.5150.75
85_I98_S0.5140.75
84_G87_R0.5140.75
93_R218_G0.5120.75
289_L293_E0.5110.75
75_G115_S0.5110.75
290_T294_R0.5110.75
17_G21_A0.5100.75
31_D61_D0.5090.75
157_F210_I0.5090.75
127_V256_I0.5090.75
288_E294_R0.5080.74
244_A253_L0.5070.74
125_N156_R0.5070.74
14_G180_H0.5060.74
21_A32_V0.5060.74
136_K139_G0.5050.74
38_P67_T0.5050.74
158_R174_G0.5050.74
135_Y140_F0.5010.73
93_R156_R0.5000.73
187_L290_T0.5000.73
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2d4aB 4 0.9679 100 0.207 Contact Map
4cl3A 4 0.984 100 0.215 Contact Map
3nepX 4 0.9519 100 0.217 Contact Map
1guzA 4 0.9744 100 0.221 Contact Map
4plzA 4 0.9519 100 0.223 Contact Map
2hjrA 4 0.9808 100 0.231 Contact Map
4rorA 4 0.984 100 0.231 Contact Map
1a5zA 3 0.9647 100 0.234 Contact Map
1lldA 3 0.9712 100 0.236 Contact Map
1pzgA 4 0.9936 100 0.237 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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