GREMLIN Database
SP3AF - Stage III sporulation protein AF
UniProt: P49783 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 206 (191)
Sequences: 334 (305)
Seq/√Len: 22.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
7_W11_I3.5851.00
137_T194_T3.0531.00
140_V182_L3.0111.00
8_L43_I2.9020.99
14_F45_V2.8810.99
104_K123_L2.8690.99
59_E156_V2.4510.98
99_M186_W2.4270.98
21_I106_K2.3630.97
123_L135_I2.2250.96
84_K94_Y2.2120.96
7_W49_P2.0560.94
27_S31_Q2.0430.94
132_E151_Q2.0050.93
12_V186_W1.9290.91
84_K87_I1.9250.91
15_I195_V1.9230.91
22_D45_V1.9210.91
138_I188_I1.9200.91
118_V135_I1.9030.91
25_L34_A1.8720.90
100_A186_W1.8670.90
25_L123_L1.6940.85
18_A42_L1.6750.84
36_M99_M1.6630.84
15_I47_L1.6410.83
42_L186_W1.6160.82
159_D162_H1.6040.82
14_F84_K1.5760.80
4_L182_L1.5470.79
117_K182_L1.5390.79
6_E54_F1.5390.79
24_L57_D1.5330.78
22_D51_F1.4970.77
50_I135_I1.4920.76
155_P195_V1.4910.76
179_Q193_I1.4890.76
43_I187_E1.4700.75
83_K86_E1.4670.75
17_F48_T1.4450.74
135_I188_I1.4330.73
172_Q175_A1.4270.73
165_V168_E1.4250.73
37_V40_L1.4180.73
34_A39_S1.4140.72
46_M111_F1.4050.72
155_P190_S1.3840.71
51_F56_T1.3780.70
42_L107_A1.3750.70
25_L138_I1.3710.70
8_L142_M1.3690.70
43_I46_M1.3450.69
41_L96_L1.3360.68
21_I33_Y1.3320.68
23_M40_L1.3240.67
58_P103_L1.3230.67
78_N89_A1.3070.66
10_T39_S1.2960.66
4_L150_V1.2920.65
111_F178_I1.2910.65
8_L39_S1.2830.65
87_I95_I1.2740.64
120_R139_S1.2710.64
122_K195_V1.2700.64
111_F118_V1.2540.63
110_R185_I1.2530.63
51_F178_I1.2510.63
12_V107_A1.2490.63
32_K44_V1.2440.62
18_A186_W1.2420.62
17_F174_E1.2340.62
33_Y182_L1.2340.62
79_Q156_V1.2300.61
92_R107_A1.2190.61
50_I157_H1.2040.60
122_K164_Y1.1980.59
93_A96_L1.1970.59
23_M60_V1.1880.59
84_K93_A1.1860.59
190_S193_I1.1560.57
151_Q154_A1.1490.56
39_S107_A1.1390.55
20_V38_V1.1330.55
157_H191_E1.1290.55
162_H192_K1.1240.54
160_T163_A1.1110.54
22_D37_V1.1010.53
93_A97_E1.0980.53
54_F142_M1.0970.53
97_E138_I1.0890.52
7_W51_F1.0880.52
34_A38_V1.0790.51
16_L65_L1.0510.49
67_K173_K1.0490.49
94_Y104_K1.0470.49
7_W111_F1.0420.49
18_A37_V1.0390.49
143_A189_G1.0390.49
188_I192_K1.0380.49
39_S46_M1.0340.48
87_I93_A1.0330.48
20_V84_K1.0220.47
4_L8_L1.0090.47
107_A186_W1.0080.47
27_S47_L1.0020.46
9_T20_V0.9930.46
162_H165_V0.9870.45
11_I54_F0.9800.45
3_F7_W0.9730.44
148_K156_V0.9630.43
176_K193_I0.9630.43
43_I58_P0.9620.43
33_Y41_L0.9600.43
14_F22_D0.9590.43
17_F188_I0.9580.43
5_T9_T0.9560.43
29_S117_K0.9530.43
43_I51_F0.9480.42
102_Q186_W0.9430.42
47_L60_V0.9430.42
138_I152_T0.9380.42
87_I111_F0.9220.41
35_K38_V0.9080.40
148_K151_Q0.9070.40
15_I113_H0.9040.40
99_M133_E0.8990.39
128_K188_I0.8920.39
10_T36_M0.8890.39
95_I98_E0.8830.38
35_K129_V0.8820.38
97_E101_V0.8810.38
15_I27_S0.8750.38
10_T25_L0.8720.38
49_P102_Q0.8640.37
20_V36_M0.8620.37
99_M107_A0.8610.37
38_V127_E0.8600.37
14_F51_F0.8590.37
161_D178_I0.8580.37
136_K151_Q0.8560.37
111_F116_Y0.8530.36
10_T52_K0.8510.36
11_I190_S0.8440.36
40_L50_I0.8400.35
28_S40_L0.8390.35
20_V133_E0.8360.35
124_T184_D0.8290.35
20_V32_K0.8240.35
20_V88_Q0.8180.34
157_H195_V0.8170.34
153_V160_T0.8140.34
124_T129_V0.8040.33
105_K156_V0.8030.33
6_E10_T0.8010.33
110_R181_Q0.8010.33
8_L99_M0.8010.33
39_S57_D0.8000.33
6_E9_T0.7970.33
125_A176_K0.7880.32
10_T24_L0.7830.32
19_I187_E0.7820.32
1_M8_L0.7780.32
168_E172_Q0.7740.31
60_V156_V0.7700.31
180_T184_D0.7680.31
188_I196_H0.7670.31
15_I45_V0.7670.31
14_F21_I0.7670.31
86_E93_A0.7630.31
51_F132_E0.7590.31
14_F106_K0.7590.31
162_H191_E0.7560.30
142_M197_M0.7500.30
142_M193_I0.7490.30
30_M156_V0.7470.30
52_K99_M0.7440.30
44_V187_E0.7440.30
164_Y195_V0.7340.29
143_A162_H0.7290.29
8_L149_T0.7280.29
16_L140_V0.7240.29
140_V178_I0.7220.29
8_L81_N0.7180.28
87_I94_Y0.7170.28
31_Q102_Q0.7170.28
1_M87_I0.7120.28
77_K125_A0.7110.28
17_F92_R0.7090.28
89_A100_A0.7070.28
124_T137_T0.7020.27
21_I153_V0.6980.27
39_S112_S0.6960.27
41_L54_F0.6930.27
18_A152_T0.6920.27
42_L113_H0.6850.27
128_K189_G0.6810.26
56_T128_K0.6790.26
116_Y174_E0.6750.26
147_E188_I0.6750.26
2_S195_V0.6730.26
7_W95_I0.6730.26
67_K151_Q0.6690.26
147_E184_D0.6680.26
86_E94_Y0.6660.26
16_L133_E0.6620.25
162_H168_E0.6620.25
58_P61_I0.6580.25
7_W84_K0.6560.25
28_S38_V0.6560.25
10_T42_L0.6560.25
133_E136_K0.6550.25
23_M185_I0.6550.25
98_E102_Q0.6540.25
60_V120_R0.6510.25
42_L190_S0.6490.25
52_K180_T0.6490.25
142_M174_E0.6470.25
60_V176_K0.6460.24
77_K153_V0.6420.24
31_Q36_M0.6410.24
140_V192_K0.6380.24
2_S177_Q0.6340.24
138_I186_W0.6330.24
42_L84_K0.6310.24
99_M132_E0.6300.24
78_N159_D0.6300.24
147_E150_V0.6280.24
24_L88_Q0.6240.23
42_L101_V0.6220.23
40_L140_V0.6210.23
4_L120_R0.6180.23
108_E147_E0.6170.23
4_L122_K0.6140.23
157_H165_V0.6120.23
43_I53_L0.6080.23
85_I88_Q0.6070.23
141_Y196_H0.6040.22
179_Q184_D0.6030.22
131_S188_I0.6020.22
88_Q168_E0.5990.22
18_A27_S0.5960.22
40_L186_W0.5960.22
16_L57_D0.5950.22
101_V162_H0.5880.22
103_L178_I0.5860.22
14_F44_V0.5820.21
117_K138_I0.5790.21
43_I61_I0.5780.21
3_F53_L0.5760.21
61_I64_Y0.5740.21
129_V144_P0.5720.21
23_M37_V0.5720.21
122_K139_S0.5700.21
52_K157_H0.5640.21
16_L186_W0.5590.20
67_K148_K0.5580.20
49_P193_I0.5540.20
81_N171_E0.5540.20
7_W102_Q0.5540.20
119_G153_V0.5510.20
47_L53_L0.5490.20
51_F181_Q0.5440.20
156_V162_H0.5410.20
16_L132_E0.5320.19
14_F98_E0.5320.19
80_I140_V0.5300.19
36_M125_A0.5270.19
167_K177_Q0.5270.19
33_Y114_D0.5260.19
30_M40_L0.5250.19
37_V150_V0.5240.19
53_L136_K0.5220.19
6_E122_K0.5210.19
65_L73_S0.5170.19
74_A193_I0.5150.18
93_A156_V0.5140.18
15_I188_I0.5140.18
107_A111_F0.5130.18
57_D100_A0.5110.18
44_V95_I0.5100.18
78_N100_A0.5090.18
27_S36_M0.5090.18
59_E133_E0.5050.18
25_L103_L0.5030.18
35_K78_N0.5010.18
29_S183_A0.5000.18
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1gyxA 2 0.2524 16.4 0.944 Contact Map
3mb2B 3 0.2573 13.9 0.946 Contact Map
3m20A 5 0.2427 12.3 0.947 Contact Map
3m21A 4 0.2621 12.1 0.947 Contact Map
2opaA 4 0.2476 11.9 0.947 Contact Map
2eelA 1 0.2961 11.6 0.947 Contact Map
3ry0A 4 0.2476 11.6 0.947 Contact Map
4x1cC 5 0.2476 11.4 0.948 Contact Map
2dzmA 1 0.301 11.4 0.948 Contact Map
4u5rA 3 0.2524 11 0.948 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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