GREMLIN Database
OPUAC - Glycine betaine-binding protein OpuAC
UniProt: P46922 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 293 (267)
Sequences: 238 (160)
Seq/√Len: 9.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
58_D113_S5.3571.00
52_T114_Y5.3311.00
152_I208_V3.7420.98
134_N187_V3.4860.97
137_K141_Q3.3540.96
138_L216_L3.1150.94
148_E176_Q3.0580.93
79_K83_D2.8730.90
125_R131_E2.6990.87
79_K102_Y2.5080.83
124_T263_D2.4630.82
92_G277_V2.4480.82
94_T201_S2.4440.81
259_K262_Y2.2750.76
179_E260_G2.1780.73
129_S136_A2.1670.73
128_F136_A2.1420.72
151_E173_H2.1250.71
255_A262_Y2.1040.70
155_K168_E2.0980.70
140_S265_I2.0920.70
30_S34_Q2.0730.69
187_V192_I2.0450.68
135_A192_I2.0290.67
43_D75_T1.9780.65
54_K58_D1.9160.63
178_A181_T1.8620.60
124_T136_A1.8520.60
112_Q116_S1.8350.59
101_R111_K1.8220.59
85_K171_N1.8100.58
44_P225_V1.7500.55
77_T231_W1.7380.55
174_K178_A1.7300.55
134_N141_Q1.6680.52
43_D226_E1.6620.51
134_N218_Y1.6610.51
134_N185_Q1.6490.51
169_Y173_H1.6470.51
195_A239_A1.6390.50
132_Q192_I1.6230.50
160_E168_E1.6200.50
35_V114_Y1.6140.49
31_A282_M1.6010.49
56_M66_L1.5940.48
145_T150_G1.5910.48
209_I243_L1.5790.48
138_L209_I1.5630.47
210_G214_E1.5590.47
134_N184_V1.5370.46
141_Q184_V1.5330.46
40_I69_A1.5030.44
129_S263_D1.5020.44
43_D71_S1.5020.44
197_V203_I1.4830.43
249_N272_V1.4760.43
147_D150_G1.4690.43
209_I216_L1.4470.42
228_G254_Y1.4470.42
72_A231_W1.4420.42
54_K101_R1.4380.41
64_W282_M1.4140.40
138_L184_V1.4120.40
72_A80_K1.4100.40
50_S253_A1.4070.40
79_K84_R1.4020.40
198_A229_P1.3950.39
167_A171_N1.3880.39
164_K168_E1.3780.39
87_P290_D1.3710.38
71_S197_V1.3640.38
76_A233_A1.3560.38
195_A233_A1.3490.38
44_P238_S1.3340.37
224_Q228_G1.3320.37
269_M272_V1.3250.36
48_I114_Y1.3220.36
120_I205_S1.3210.36
123_I242_S1.3200.36
119_E270_T1.3040.36
149_M205_S1.3030.36
138_L170_V1.3000.35
35_V52_T1.2870.35
35_V64_W1.2820.35
255_A259_K1.2780.35
66_L98_M1.2580.34
124_T242_S1.2320.33
88_I220_V1.2200.32
140_S255_A1.2100.32
55_A113_S1.2070.32
282_M285_V1.2060.32
226_E232_T1.2030.32
233_A239_A1.2030.32
114_Y242_S1.1970.31
196_Y203_I1.1940.31
71_S198_A1.1880.31
170_V223_T1.1870.31
137_K184_V1.1830.31
69_A272_V1.1810.31
58_D102_Y1.1790.31
85_K108_D1.1770.31
179_E182_K1.1710.30
192_I239_A1.1480.29
128_F268_S1.1470.29
75_T226_E1.1280.29
121_H264_D1.1220.28
124_T128_F1.1170.28
64_W87_P1.1140.28
210_G222_L1.1120.28
32_A64_W1.1090.28
173_H176_Q1.1010.28
132_Q187_V1.0950.27
57_K182_K1.0900.27
172_K186_K1.0890.27
252_K256_A1.0870.27
71_S75_T1.0860.27
69_A89_I1.0850.27
224_Q292_K1.0840.27
232_T254_Y1.0790.27
197_V227_A1.0770.27
211_K214_E1.0730.27
194_L202_E1.0700.27
229_P232_T1.0690.27
75_T227_A1.0690.27
199_W246_W1.0660.26
265_I268_S1.0590.26
181_T206_T1.0520.26
124_T241_A1.0490.26
198_A203_I1.0390.25
191_K221_T1.0300.25
233_A253_A1.0290.25
227_A251_H1.0230.25
38_T67_I1.0220.25
56_M113_S1.0220.25
108_D130_K1.0070.24
149_M201_S1.0070.24
171_N174_K1.0020.24
84_R280_Q1.0020.24
205_S220_V0.9930.24
68_S233_A0.9920.24
140_S169_Y0.9870.24
131_E165_V0.9820.24
184_V241_A0.9750.23
49_M66_L0.9650.23
140_S268_S0.9540.23
60_D219_E0.9540.23
129_S192_I0.9500.23
128_F269_M0.9400.22
213_L218_Y0.9390.22
198_A234_I0.9380.22
148_E208_V0.9300.22
155_K160_E0.9290.22
166_A208_V0.9270.22
31_A34_Q0.9250.22
128_F131_E0.9220.22
83_D130_K0.9170.22
118_E250_T0.9170.22
49_M63_D0.9160.21
135_A187_V0.9150.21
198_A235_A0.9140.21
69_A84_R0.9130.21
193_N223_T0.9100.21
124_T209_I0.9070.21
45_G180_W0.9010.21
184_V211_K0.8990.21
113_S128_F0.8980.21
46_S200_D0.8930.21
55_A166_A0.8920.21
34_Q173_H0.8910.21
168_E171_N0.8900.21
69_A226_E0.8890.21
144_W288_I0.8890.21
85_K126_K0.8870.21
32_A63_D0.8850.21
37_K64_W0.8840.21
170_V208_V0.8830.20
247_L262_Y0.8750.20
103_K214_E0.8720.20
182_K282_M0.8690.20
106_Y150_G0.8650.20
140_S266_G0.8620.20
75_T121_H0.8590.20
200_D205_S0.8580.20
132_Q190_D0.8550.20
249_N274_M0.8540.20
48_I225_V0.8530.20
100_S251_H0.8530.20
76_A272_V0.8470.19
149_M244_S0.8450.19
49_M196_Y0.8450.19
259_K263_D0.8450.19
141_Q170_V0.8440.19
194_L241_A0.8370.19
26_D29_A0.8340.19
285_V289_E0.8320.19
35_V89_I0.8270.19
65_T223_T0.8240.19
49_M254_Y0.8230.19
135_A194_L0.8190.19
208_V255_A0.8180.19
169_Y265_I0.8160.19
43_D198_A0.8130.19
60_D193_N0.8130.19
124_T234_I0.8120.18
34_Q84_R0.8100.18
132_Q218_Y0.8070.18
245_A258_Y0.8060.18
43_D282_M0.8040.18
55_A201_S0.8000.18
56_M103_K0.7980.18
27_E219_E0.7960.18
28_N137_K0.7950.18
103_K116_S0.7930.18
259_K273_K0.7900.18
161_K292_K0.7880.18
66_L97_W0.7870.18
260_G282_M0.7840.18
85_K188_K0.7820.18
147_D187_V0.7810.18
198_A231_W0.7790.18
52_T55_A0.7730.17
124_T140_S0.7710.17
228_G232_T0.7680.17
146_Q150_G0.7680.17
76_A80_K0.7680.17
137_K181_T0.7670.17
119_E261_K0.7650.17
198_A226_E0.7610.17
49_M68_S0.7600.17
40_I272_V0.7600.17
126_K137_K0.7530.17
169_Y266_G0.7530.17
38_T233_A0.7490.17
167_A211_K0.7480.17
48_I51_L0.7470.17
196_Y206_T0.7420.17
169_Y176_Q0.7410.17
72_A130_K0.7410.17
49_M227_A0.7400.17
243_L268_S0.7400.17
234_I239_A0.7390.17
36_N86_K0.7380.17
227_A231_W0.7380.17
35_V40_I0.7340.16
116_S172_K0.7310.16
25_N29_A0.7300.16
130_K188_K0.7280.16
206_T254_Y0.7230.16
120_I149_M0.7200.16
167_A170_V0.7170.16
198_A227_A0.7160.16
83_D98_M0.7160.16
201_S205_S0.7160.16
226_E229_P0.7140.16
198_A207_N0.7120.16
65_T193_N0.7090.16
267_T280_Q0.7080.16
71_S147_D0.7070.16
98_M273_K0.7060.16
89_I157_E0.7030.16
233_A272_V0.7000.16
182_K186_K0.7000.16
72_A227_A0.6990.16
49_M198_A0.6990.16
225_V277_V0.6910.15
43_D146_Q0.6880.15
131_E192_I0.6840.15
142_F204_A0.6840.15
53_D68_S0.6820.15
113_S274_M0.6760.15
237_G251_H0.6730.15
179_E215_D0.6670.15
44_P48_I0.6640.15
101_R108_D0.6630.15
37_K239_A0.6620.15
98_M188_K0.6610.15
44_P157_E0.6610.15
37_K40_I0.6610.15
235_A264_D0.6600.15
131_E166_A0.6580.15
69_A82_Y0.6540.15
98_M172_K0.6520.14
62_N157_E0.6490.14
49_M206_T0.6480.14
94_T106_Y0.6450.14
69_A225_V0.6420.14
174_K209_I0.6410.14
35_V257_K0.6370.14
139_L165_V0.6350.14
196_Y207_N0.6280.14
141_Q178_A0.6250.14
185_Q280_Q0.6240.14
227_A230_M0.6200.14
193_N292_K0.6170.14
123_I262_Y0.6160.14
32_A37_K0.6150.14
72_A123_I0.6150.14
79_K241_A0.6150.14
81_S165_V0.6100.14
97_W204_A0.6090.14
162_P197_V0.6060.13
128_F234_I0.6060.13
121_H226_E0.6050.13
113_S258_Y0.6030.13
197_V229_P0.6000.13
51_L274_M0.5990.13
164_K173_H0.5970.13
97_W142_F0.5970.13
53_D56_M0.5970.13
246_W266_G0.5960.13
31_A124_T0.5950.13
170_V177_I0.5950.13
36_N183_G0.5930.13
33_E63_D0.5930.13
114_Y276_L0.5930.13
243_L269_M0.5920.13
45_G120_I0.5910.13
126_K171_N0.5910.13
71_S227_A0.5900.13
44_P83_D0.5890.13
57_K186_K0.5890.13
60_D192_I0.5880.13
97_W102_Y0.5870.13
255_A260_G0.5850.13
48_I274_M0.5840.13
141_Q183_G0.5810.13
112_Q261_K0.5810.13
111_K151_E0.5800.13
209_I242_S0.5780.13
210_G229_P0.5780.13
186_K216_L0.5770.13
170_V201_S0.5750.13
60_D158_E0.5740.13
149_M226_E0.5730.13
52_T276_L0.5710.13
120_I273_K0.5710.13
196_Y222_L0.5670.13
43_D121_H0.5670.13
134_N137_K0.5660.13
220_V271_G0.5640.13
56_M130_K0.5630.13
65_T190_D0.5610.13
124_T243_L0.5580.13
67_I76_A0.5570.12
202_E243_L0.5550.12
243_L272_V0.5550.12
182_K286_N0.5490.12
36_N286_N0.5480.12
58_D111_K0.5460.12
40_I154_I0.5440.12
75_T198_A0.5440.12
43_D227_A0.5440.12
225_V238_S0.5440.12
148_E177_I0.5420.12
33_E175_D0.5420.12
32_A226_E0.5400.12
156_V159_G0.5400.12
51_L226_E0.5390.12
40_I185_Q0.5380.12
56_M175_D0.5370.12
26_D234_I0.5350.12
34_Q249_N0.5350.12
123_I186_K0.5350.12
187_V190_D0.5340.12
89_I224_Q0.5340.12
137_K218_Y0.5320.12
89_I105_K0.5310.12
102_Y157_E0.5310.12
48_I52_T0.5310.12
84_R292_K0.5290.12
140_S269_M0.5290.12
93_W246_W0.5280.12
117_A152_I0.5280.12
144_W202_E0.5260.12
175_D286_N0.5260.12
228_G237_G0.5240.12
106_Y273_K0.5240.12
116_S274_M0.5240.12
85_K182_K0.5230.12
71_S285_V0.5170.12
141_Q192_I0.5150.12
126_K261_K0.5130.12
92_G153_M0.5130.12
230_M244_S0.5110.12
141_Q211_K0.5110.12
45_G93_W0.5090.12
144_W166_A0.5090.12
40_I67_I0.5080.12
27_E280_Q0.5080.12
146_Q196_Y0.5080.12
83_D90_I0.5080.12
64_W216_L0.5060.12
48_I152_I0.5060.12
197_V207_N0.5040.11
176_Q285_V0.5030.11
37_K87_P0.5020.11
196_Y204_A0.5020.11
267_T292_K0.5010.11
143_K221_T0.5010.11
185_Q192_I0.5000.11
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2b4lA 1 0.9147 100 0.49 Contact Map
1r9lA 1 0.6451 100 0.798 Contact Map
4ne4A 1 0.6007 99.9 0.815 Contact Map
3o66A 1 0.5734 99.9 0.817 Contact Map
2rinA 1 0.686 99.9 0.823 Contact Map
3l6gA 1 0.6246 99.9 0.829 Contact Map
3r6uA 1 0.5734 99.9 0.841 Contact Map
3tmgA 1 0.6348 99.8 0.851 Contact Map
1sw5A 1 0.5836 99.8 0.858 Contact Map
1p99A 1 0.3242 98.8 0.919 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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