YQBT - BACSU - GREMLIN Contacts

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YQBT - Uncharacterized protein YqbT

UniProt:	P45935	Sequences: MFEDQTYESIMDRMLNSISADIDKREGSVIYNALAPVAAELAKSYIWLDTVLELVFSDTAQGEFLDRRATEAGIERTAATKAVRAGEFTEGVTIPVGSRFYVDNLYFQYTADGTLECETAGEAGNANISGQNLLSLDTIPGLQKAIVKEILIPGREEEDDDSLRARYFTRVRREAVSANKAHYKQWAEEVDGVGKVKVFPLWNGDGTVKIVVTNANLEPASDILISKVKNYIDPEPGQGEGQAPIGAFVTVESAVWKEVEISAEVLPEVNSSIDQVKQEIESGVLNLFKKIAFEDNVIRLSQINNIVYNSPSVSDYADIKINGVAENLVLSAVEIPKLGQVNIIEQTR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	348 (324)
Sequences:	2534 (2196)
Seq/√Len:	122.0

ECOLI - JAYE
BACSU - YQBT XKDT

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
69_A	163_L	4.579	1.00
84_R	151_L	4.095	1.00
222_D	226_S	3.898	1.00
66_D	76_R	3.697	1.00
257_K	334_E	3.690	1.00
158_E	166_R	3.688	1.00
280_I	307_V	3.659	1.00
331_S	334_E	3.280	1.00
62_G	160_D	3.203	1.00
159_D	162_S	2.996	1.00
87_E	148_K	2.945	1.00
86_G	100_F	2.870	1.00
255_V	334_E	2.767	1.00
110_T	116_E	2.765	1.00
69_A	167_Y	2.682	1.00
6_T	9_S	2.655	1.00
233_D	243_A	2.604	1.00
81_K	116_E	2.561	1.00
99_R	106_Y	2.529	1.00
184_K	188_E	2.464	1.00
298_I	336_P	2.460	1.00
184_K	198_V	2.339	1.00
86_G	115_L	2.338	1.00
277_K	281_E	2.323	1.00
260_E	339_G	2.323	1.00
330_L	336_P	2.262	1.00
220_A	253_S	2.256	1.00
11_M	34_L	2.175	1.00
122_E	156_E	2.172	1.00
281_E	285_L	2.108	1.00
265_V	280_I	2.107	1.00
76_R	160_D	2.088	1.00
93_T	112_D	2.078	1.00
315_D	327_N	2.005	1.00
263_A	307_V	1.991	1.00
88_F	100_F	1.971	1.00
108_Q	116_E	1.943	1.00
66_D	160_D	1.891	1.00
79_A	120_A	1.876	1.00
132_N	147_V	1.861	1.00
68_R	71_E	1.851	1.00
100_F	109_Y	1.827	1.00
89_T	144_K	1.818	1.00
188_E	196_V	1.811	1.00
199_F	211_V	1.768	1.00
107_F	126_A	1.722	1.00
65_L	164_R	1.709	1.00
117_C	123_A	1.706	1.00
187_A	210_I	1.693	1.00
76_R	163_L	1.690	1.00
301_S	304_N	1.689	1.00
82_A	124_G	1.687	1.00
304_N	316_Y	1.686	1.00
189_E	231_Y	1.675	1.00
209_K	252_E	1.642	1.00
99_R	108_Q	1.621	1.00
274_D	277_K	1.609	1.00
117_C	124_G	1.598	1.00
65_L	160_D	1.587	1.00
187_A	228_V	1.586	1.00
204_G	207_T	1.564	1.00
88_F	94_I	1.549	1.00
99_R	137_D	1.548	1.00
127_N	130_G	1.541	1.00
82_A	125_N	1.536	1.00
210_I	232_I	1.523	1.00
282_S	286_N	1.518	1.00
169_T	188_E	1.515	1.00
225_I	253_S	1.509	1.00
283_G	310_S	1.509	1.00
186_W	231_Y	1.506	1.00
108_Q	118_E	1.458	1.00
226_S	230_N	1.452	1.00
86_G	145_A	1.429	1.00
183_Y	208_V	1.427	1.00
13_R	40_E	1.419	1.00
302_Q	306_I	1.411	1.00
74_I	163_L	1.406	1.00
12_D	16_N	1.396	1.00
88_F	135_S	1.394	1.00
105_L	124_G	1.378	1.00
275_Q	279_E	1.371	1.00
88_F	109_Y	1.361	1.00
95_P	98_S	1.357	1.00
121_G	125_N	1.348	1.00
11_M	35_A	1.346	1.00
14_M	38_A	1.336	1.00
31_Y	35_A	1.333	1.00
258_E	339_G	1.325	1.00
212_V	228_V	1.313	1.00
211_V	252_E	1.309	1.00
133_L	147_V	1.306	1.00
15_L	31_Y	1.302	1.00
62_G	76_R	1.301	1.00
287_L	306_I	1.298	1.00
288_F	338_L	1.295	1.00
258_E	337_K	1.288	1.00
79_A	157_E	1.284	1.00
187_A	196_V	1.276	1.00
129_S	146_I	1.270	1.00
106_Y	118_E	1.261	1.00
161_D	164_R	1.258	1.00
109_Y	113_G	1.241	0.99
267_P	276_V	1.240	0.99
231_Y	242_Q	1.235	0.99
272_S	275_Q	1.227	0.99
130_G	133_L	1.226	0.99
115_L	151_L	1.224	0.99
221_S	224_L	1.216	0.99
190_V	228_V	1.215	0.99
98_S	109_Y	1.213	0.99
180_K	200_P	1.212	0.99
125_N	154_G	1.204	0.99
178_A	244_P	1.202	0.99
102_V	105_L	1.198	0.99
54_L	60_A	1.193	0.99
56_F	59_T	1.188	0.99
229_K	233_D	1.188	0.99
75_E	163_L	1.184	0.99
291_I	294_E	1.183	0.99
97_G	108_Q	1.181	0.99
94_I	139_I	1.177	0.99
128_I	150_I	1.176	0.99
214_N	224_L	1.168	0.99
225_I	229_K	1.162	0.99
62_G	66_D	1.158	0.99
278_Q	281_E	1.154	0.99
100_F	135_S	1.143	0.99
88_F	98_S	1.136	0.99
260_E	340_Q	1.118	0.99
187_A	190_V	1.109	0.99
229_K	249_V	1.106	0.99
126_A	151_L	1.105	0.99
54_L	64_F	1.105	0.99
78_A	157_E	1.102	0.99
102_V	126_A	1.094	0.99
84_R	115_L	1.093	0.99
278_Q	282_S	1.091	0.99
126_A	132_N	1.089	0.99
126_A	147_V	1.085	0.99
96_V	112_D	1.083	0.99
184_K	197_K	1.054	0.98
300_L	321_I	1.047	0.98
14_M	34_L	1.035	0.98
310_S	313_V	1.026	0.98
57_S	68_R	1.023	0.98
23_D	28_S	1.017	0.98
8_E	12_D	1.014	0.98
50_T	54_L	1.014	0.98
88_F	145_A	1.013	0.98
210_I	251_V	1.005	0.98
224_L	228_V	1.005	0.98
201_L	207_T	1.005	0.98
234_P	242_Q	0.996	0.97
50_T	61_Q	0.995	0.97
330_L	334_E	0.988	0.97
7_Y	11_M	0.984	0.97
84_R	132_N	0.980	0.97
212_V	225_I	0.979	0.97
58_D	67_R	0.978	0.97
262_S	320_K	0.975	0.97
297_V	329_V	0.974	0.97
98_S	142_L	0.971	0.97
176_V	179_N	0.969	0.97
259_V	298_I	0.965	0.97
268_E	314_S	0.960	0.97
261_I	288_F	0.957	0.97
83_V	116_E	0.954	0.97
160_D	163_L	0.954	0.97
42_A	46_I	0.946	0.97
177_S	182_H	0.941	0.96
81_K	120_A	0.941	0.96
297_V	330_L	0.935	0.96
132_N	151_L	0.935	0.96
69_A	74_I	0.934	0.96
41_L	44_S	0.928	0.96
224_L	227_K	0.926	0.96
21_D	24_K	0.926	0.96
87_E	146_I	0.923	0.96
301_S	308_Y	0.920	0.96
10_I	13_R	0.919	0.96
257_K	330_L	0.917	0.96
220_A	224_L	0.917	0.96
105_L	117_C	0.915	0.96
313_V	316_Y	0.909	0.96
101_Y	136_L	0.909	0.96
262_S	322_N	0.907	0.96
93_T	114_T	0.902	0.95
83_V	110_T	0.900	0.95
193_V	212_V	0.899	0.95
95_P	112_D	0.898	0.95
300_L	304_N	0.896	0.95
10_I	40_E	0.895	0.95
82_A	151_L	0.886	0.95
21_D	30_I	0.883	0.95
74_I	167_Y	0.881	0.95
68_R	72_A	0.879	0.95
101_Y	106_Y	0.878	0.95
48_L	51_V	0.878	0.95
106_Y	136_L	0.876	0.95
185_Q	189_E	0.874	0.95
72_A	167_Y	0.872	0.95
299_R	315_D	0.871	0.94
146_I	150_I	0.869	0.94
184_K	195_K	0.865	0.94
9_S	13_R	0.864	0.94
183_Y	186_W	0.855	0.94
176_V	182_H	0.853	0.94
207_T	250_T	0.852	0.94
135_S	142_L	0.851	0.94
45_Y	48_L	0.846	0.94
182_H	186_W	0.841	0.93
213_T	335_I	0.841	0.93
234_P	243_A	0.841	0.93
283_G	306_I	0.840	0.93
202_W	209_K	0.838	0.93
77_T	162_S	0.835	0.93
123_A	156_E	0.834	0.93
57_S	164_R	0.832	0.93
305_N	308_Y	0.827	0.93
41_L	45_Y	0.827	0.93
163_L	167_Y	0.826	0.93
212_V	251_V	0.824	0.93
181_A	184_K	0.818	0.92
13_R	17_S	0.817	0.92
243_A	249_V	0.812	0.92
85_A	149_E	0.810	0.92
72_A	171_V	0.808	0.92
88_F	142_L	0.808	0.92
59_T	64_F	0.806	0.92
27_G	31_Y	0.806	0.92
67_R	70_T	0.803	0.92
29_V	32_N	0.800	0.92
183_Y	244_P	0.798	0.91
63_E	67_R	0.793	0.91
276_V	310_S	0.793	0.91
14_M	41_L	0.788	0.91
57_S	167_Y	0.788	0.91
82_A	107_F	0.786	0.91
100_F	147_V	0.786	0.91
302_Q	305_N	0.786	0.91
192_G	215_A	0.785	0.91
57_S	71_E	0.784	0.91
300_L	327_N	0.784	0.91
276_V	279_E	0.784	0.91
228_V	251_V	0.783	0.91
14_M	37_V	0.782	0.91
132_N	145_A	0.782	0.91
37_V	51_V	0.778	0.90
225_I	251_V	0.778	0.90
231_Y	234_P	0.776	0.90
276_V	312_S	0.775	0.90
188_E	195_K	0.774	0.90
284_V	307_V	0.774	0.90
287_L	291_I	0.770	0.90
11_M	15_L	0.769	0.90
214_N	221_S	0.768	0.90
117_C	121_G	0.763	0.90
260_E	322_N	0.763	0.90
65_L	163_L	0.760	0.89
178_A	186_W	0.759	0.89
243_A	246_G	0.759	0.89
268_E	271_S	0.758	0.89
281_E	340_Q	0.757	0.89
296_N	302_Q	0.751	0.89
202_W	207_T	0.746	0.88
209_K	333_V	0.744	0.88
203_N	209_K	0.742	0.88
84_R	150_I	0.742	0.88
58_D	70_T	0.739	0.88
285_L	339_G	0.734	0.88
72_A	170_R	0.734	0.88
211_V	333_V	0.734	0.88
286_N	290_K	0.731	0.87
26_E	35_A	0.727	0.87
27_G	32_N	0.725	0.87
77_T	155_R	0.722	0.87
81_K	119_T	0.721	0.87
116_E	119_T	0.720	0.87
52_L	56_F	0.718	0.87
54_L	61_Q	0.718	0.87
98_S	135_S	0.715	0.86
190_V	231_Y	0.713	0.86
183_Y	247_A	0.711	0.86
187_A	198_V	0.710	0.86
110_T	113_G	0.709	0.86
187_A	232_I	0.709	0.86
135_S	139_I	0.708	0.86
82_A	153_P	0.701	0.85
171_V	174_E	0.699	0.85
128_I	148_K	0.699	0.85
36_P	39_A	0.698	0.85
60_A	164_R	0.697	0.85
186_W	189_E	0.697	0.85
105_L	119_T	0.696	0.85
225_I	254_A	0.694	0.85
18_I	30_I	0.691	0.84
302_Q	308_Y	0.690	0.84
6_T	45_Y	0.689	0.84
256_W	337_K	0.689	0.84
80_T	155_R	0.687	0.84
28_S	31_Y	0.687	0.84
18_I	35_A	0.686	0.84
115_L	145_A	0.685	0.84
128_I	149_E	0.684	0.84
92_V	139_I	0.683	0.84
287_L	290_K	0.681	0.83
323_G	334_E	0.681	0.83
265_V	313_V	0.676	0.83
11_M	14_M	0.674	0.83
220_A	225_I	0.674	0.83
187_A	212_V	0.673	0.83
69_A	171_V	0.673	0.83
263_A	280_I	0.672	0.83
183_Y	187_A	0.670	0.82
194_G	213_T	0.669	0.82
305_N	309_N	0.669	0.82
283_G	309_N	0.668	0.82
66_D	163_L	0.667	0.82
195_K	213_T	0.666	0.82
107_F	151_L	0.662	0.82
159_D	163_L	0.661	0.82
10_I	45_Y	0.661	0.82
17_S	39_A	0.660	0.82
285_L	289_K	0.660	0.82
30_I	33_A	0.659	0.81
179_N	182_H	0.659	0.81
19_S	22_I	0.656	0.81
119_T	124_G	0.656	0.81
285_L	338_L	0.655	0.81
102_V	132_N	0.654	0.81
56_F	60_A	0.652	0.81
49_D	52_L	0.649	0.80
159_D	164_R	0.648	0.80
84_R	148_K	0.646	0.80
223_I	227_K	0.643	0.80
289_K	293_F	0.643	0.80
302_Q	315_D	0.641	0.80
77_T	166_R	0.641	0.80
58_D	168_F	0.640	0.80
196_V	210_I	0.639	0.79
190_V	227_K	0.637	0.79
279_E	282_S	0.636	0.79
173_R	181_A	0.636	0.79
105_L	123_A	0.635	0.79
57_S	65_L	0.634	0.79
94_I	157_E	0.634	0.79
267_P	270_N	0.633	0.79
175_A	242_Q	0.633	0.79
177_S	186_W	0.632	0.79
98_S	139_I	0.632	0.79
220_A	228_V	0.632	0.79
259_V	336_P	0.631	0.79
165_A	169_T	0.631	0.79
13_R	16_N	0.630	0.78
15_L	24_K	0.629	0.78
182_H	185_Q	0.629	0.78
301_S	316_Y	0.628	0.78
33_A	36_P	0.624	0.78
82_A	121_G	0.622	0.78
202_W	250_T	0.622	0.78
56_F	167_Y	0.619	0.77
39_A	43_K	0.618	0.77
252_E	333_V	0.617	0.77
167_Y	171_V	0.617	0.77
24_K	31_Y	0.615	0.77
280_I	283_G	0.614	0.77
206_G	225_I	0.611	0.76
95_P	109_Y	0.611	0.76
194_G	215_A	0.608	0.76
22_I	25_R	0.605	0.76
63_E	66_D	0.604	0.76
190_V	242_Q	0.603	0.75
32_N	35_A	0.601	0.75
109_Y	115_L	0.600	0.75
99_R	136_L	0.598	0.75
84_R	153_P	0.593	0.74
209_K	250_T	0.593	0.74
82_A	122_E	0.591	0.74
298_I	330_L	0.591	0.74
83_V	152_I	0.590	0.74
17_S	20_A	0.589	0.74
101_Y	104_N	0.588	0.74
267_P	277_K	0.587	0.73
76_R	157_E	0.586	0.73
107_F	147_V	0.586	0.73
147_V	150_I	0.586	0.73
65_L	167_Y	0.585	0.73
126_A	130_G	0.585	0.73
49_D	53_E	0.583	0.73
23_D	48_L	0.583	0.73
226_S	229_K	0.581	0.73
14_M	17_S	0.579	0.72
29_V	33_A	0.579	0.72
222_D	253_S	0.579	0.72
70_T	76_R	0.576	0.72
33_A	37_V	0.576	0.72
287_L	303_I	0.573	0.72
15_L	35_A	0.571	0.71
77_T	158_E	0.570	0.71
40_E	43_K	0.569	0.71
252_E	303_I	0.568	0.71
195_K	294_E	0.566	0.71
121_G	156_E	0.566	0.71
50_T	53_E	0.564	0.71
111_A	114_T	0.559	0.70
78_A	301_S	0.557	0.70
92_V	95_P	0.556	0.69
170_R	175_A	0.555	0.69
47_W	51_V	0.555	0.69
299_R	327_N	0.554	0.69
213_T	254_A	0.554	0.69
51_V	55_V	0.552	0.69
17_S	40_E	0.552	0.69
271_S	312_S	0.550	0.69
27_G	35_A	0.549	0.68
219_P	256_W	0.549	0.68
320_K	324_V	0.548	0.68
300_L	324_V	0.547	0.68
44_S	47_W	0.546	0.68
337_K	340_Q	0.545	0.68
321_I	330_L	0.545	0.68
92_V	141_G	0.543	0.68
29_V	56_F	0.542	0.67
147_V	151_L	0.542	0.67
212_V	224_L	0.540	0.67
221_S	228_V	0.538	0.67
60_A	69_A	0.538	0.67
172_R	181_A	0.537	0.67
299_R	304_N	0.537	0.67
19_S	24_K	0.536	0.67
7_Y	39_A	0.536	0.67
60_A	65_L	0.535	0.66
12_D	309_N	0.534	0.66
43_K	46_I	0.533	0.66
229_K	251_V	0.533	0.66
188_E	191_D	0.530	0.66
96_V	110_T	0.529	0.66
86_G	147_V	0.528	0.65
286_N	289_K	0.527	0.65
135_S	145_A	0.527	0.65
100_F	133_L	0.527	0.65
193_V	196_V	0.526	0.65
175_A	182_H	0.526	0.65
264_E	320_K	0.525	0.65
10_I	41_L	0.525	0.65
186_W	232_I	0.525	0.65
210_I	228_V	0.524	0.65
255_V	333_V	0.524	0.65
129_S	148_K	0.522	0.65
289_K	294_E	0.522	0.65
214_N	256_W	0.521	0.64
55_V	68_R	0.520	0.64
200_P	204_G	0.520	0.64
283_G	286_N	0.518	0.64
18_I	24_K	0.518	0.64
92_V	114_T	0.518	0.64
115_L	133_L	0.516	0.64
32_N	36_P	0.516	0.64
221_S	231_Y	0.515	0.64
39_A	46_I	0.515	0.64
100_F	151_L	0.515	0.64
170_R	174_E	0.515	0.64
284_V	308_Y	0.512	0.63
128_I	147_V	0.511	0.63
137_D	226_S	0.509	0.63
81_K	110_T	0.509	0.63
251_V	303_I	0.509	0.63
302_Q	309_N	0.508	0.63
181_A	185_Q	0.508	0.63
52_L	71_E	0.508	0.63
76_R	166_R	0.507	0.62
166_R	189_E	0.506	0.62
194_G	214_N	0.506	0.62
49_D	64_F	0.506	0.62
52_L	55_V	0.506	0.62
94_I	142_L	0.505	0.62
298_I	327_N	0.505	0.62
80_T	110_T	0.504	0.62
223_I	336_P	0.504	0.62
78_A	292_A	0.502	0.62
101_Y	134_L	0.502	0.62
77_T	189_E	0.502	0.62
292_A	336_P	0.501	0.61
28_S	59_T	0.501	0.61
102_V	147_V	0.500	0.61

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3h2tA	2	0.4511	98.6	0.855	Contact Map
3laxA	1	0.2845	41.6	0.951	Contact Map
2y27A	2	0.7328	18.3	0.959	Contact Map
3zieA	2	0.2126	13.9	0.961	Contact Map
3p04A	2	0.1897	13.7	0.961	Contact Map
3zigA	2	0.1925	10.5	0.963	Contact Map
2y4oA	2	0.7241	6.9	0.966	Contact Map
1mdbA	1	0.7385	6.7	0.966	Contact Map
3v9oA	3	0.3247	6.1	0.967	Contact Map
2k6lA	2	0.1466	5.6	0.968	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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