GREMLIN Database
RF1 - Peptide chain release factor 1
UniProt: P45872 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 356 (334)
Sequences: 5967 (3109)
Seq/√Len: 170.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
280_F283_E4.4171.00
278_D282_Q3.2191.00
282_Q286_A3.0831.00
141_Q336_D2.9891.00
11_R37_E2.9851.00
112_M163_F2.9581.00
279_K283_E2.7681.00
310_F331_L2.7461.00
9_E49_R2.7171.00
225_F254_S2.6521.00
138_A165_I2.6361.00
56_E60_D2.5781.00
109_N207_E2.4991.00
222_V244_L2.4981.00
135_S145_T2.4931.00
27_D30_K2.4751.00
326_K329_Q2.4401.00
226_A239_M2.4361.00
220_I244_L2.4221.00
220_I269_M2.3701.00
50_Q87_E2.3651.00
131_Y163_F2.3511.00
15_L34_Y2.3201.00
221_R247_L2.2161.00
283_E287_E2.1931.00
244_L269_M2.1871.00
262_I266_E2.1361.00
23_E26_N2.1201.00
141_Q173_K2.0841.00
131_Y161_I2.0831.00
330_I338_V2.0491.00
30_K33_E2.0401.00
230_P234_S2.0251.00
14_K18_L2.0031.00
227_S234_S1.9931.00
118_A199_T1.9581.00
57_Q60_D1.9471.00
62_K85_Q1.9031.00
146_E166_T1.8951.00
42_Q46_D1.8801.00
329_Q334_K1.8701.00
281_Q285_Q1.8521.00
114_I127_A1.8451.00
18_L30_K1.8281.00
130_L200_A1.8131.00
230_P259_K1.8121.00
87_E90_T1.7961.00
137_Y336_D1.7831.00
131_Y135_S1.7791.00
58_L81_I1.7501.00
53_D57_Q1.7371.00
234_S238_T1.7341.00
277_Y281_Q1.7321.00
190_E194_R1.7311.00
322_L337_E1.7201.00
285_Q289_D1.7001.00
234_S259_K1.6721.00
223_D245_T1.6671.00
18_L21_D1.6451.00
281_Q284_A1.6441.00
258_E263_K1.6381.00
83_E87_E1.6371.00
109_N166_T1.6331.00
16_N42_Q1.6291.00
145_T163_F1.6211.00
234_S240_S1.6091.00
146_E164_M1.5981.00
190_E196_H1.5881.00
19_L35_S1.5781.00
38_Q42_Q1.5641.00
16_N38_Q1.5471.00
13_E16_N1.5471.00
86_K90_T1.5341.00
258_E267_K1.5221.00
150_A158_Y1.5141.00
131_Y147_V1.4981.00
234_S237_T1.4841.00
245_T252_V1.4821.00
310_F327_L1.4811.00
222_V265_K1.4711.00
315_V338_V1.4451.00
144_K166_T1.4361.00
280_F284_A1.4321.00
257_D267_K1.4101.00
132_R332_E1.4051.00
54_A87_E1.4021.00
227_S237_T1.4011.00
266_E270_K1.3811.00
224_T242_V1.3811.00
29_K33_E1.3741.00
48_Y52_R1.3691.00
284_A288_Y1.3691.00
271_V275_R1.3551.00
147_V161_I1.3421.00
287_E290_Q1.3411.00
224_T239_M1.3361.00
138_A145_T1.3331.00
47_V98_L1.3181.00
221_R245_T1.3111.00
267_K270_K1.2951.00
220_I247_L1.2921.00
79_E83_E1.2901.00
114_I161_I1.2851.00
324_I330_I1.2831.00
29_K32_R1.2831.00
140_L334_K1.2701.00
211_V277_Y1.2581.00
54_A88_T1.2541.00
251_V279_K1.2381.00
262_I265_K1.2371.00
143_W174_L1.2361.00
282_Q285_Q1.2311.00
327_L331_L1.2301.00
313_N328_D1.2211.00
186_V193_G1.1971.00
19_L24_V1.1941.00
12_Y38_Q1.1901.00
31_L35_S1.1861.00
75_D79_E1.1731.00
330_I335_L1.1681.00
307_T318_H1.1671.00
315_V330_I1.1651.00
121_E153_T1.1651.00
164_M207_E1.1621.00
136_R332_E1.1561.00
11_R14_K1.1541.00
107_D169_G1.1371.00
46_D50_Q1.1351.00
285_Q288_Y1.1311.00
199_T298_S1.1311.00
76_M80_E1.1301.00
74_R78_K1.1201.00
263_K270_K1.1181.00
28_P31_L1.1101.00
111_I164_M1.1071.00
241_A256_Q1.0991.00
114_I202_V1.0951.00
111_I148_M1.0901.00
21_D24_V1.0881.00
85_Q89_E1.0811.00
173_K340_E1.0811.00
286_A290_Q1.0781.00
47_V50_Q1.0781.00
324_I337_E1.0731.00
110_V172_S1.0701.00
135_S163_F1.0621.00
234_S239_M1.0621.00
322_L341_A1.0591.00
217_E220_I1.0570.99
190_E193_G1.0500.99
296_V303_E1.0490.99
140_L332_E1.0430.99
225_F243_R1.0420.99
324_I327_L1.0410.99
49_R52_R1.0390.99
326_K337_E1.0390.99
50_Q54_A1.0350.99
112_M165_I1.0330.99
127_A200_A1.0260.99
224_T234_S1.0240.99
246_H250_G1.0240.99
165_I174_L1.0200.99
46_D49_R1.0180.99
60_D63_A1.0160.99
284_A287_E1.0150.99
73_M77_V1.0140.99
51_Y55_S1.0070.99
158_Y161_I1.0070.99
13_E17_E1.0050.99
129_N310_F1.0020.99
105_N108_K1.0010.99
289_D293_K1.0000.99
215_I269_M0.9930.99
52_R56_E0.9930.99
28_P32_R0.9910.99
337_E341_A0.9900.99
137_Y173_K0.9880.99
125_L155_T0.9850.99
54_A91_L0.9770.99
11_R18_L0.9740.99
176_Y342_L0.9710.99
283_E286_A0.9690.99
254_S257_D0.9660.99
75_D78_K0.9650.99
178_N203_A0.9560.99
54_A80_E0.9480.99
173_K336_D0.9470.99
45_V49_R0.9460.99
203_A292_R0.9440.99
76_M79_E0.9430.99
82_S85_Q0.9380.99
251_V276_I0.9340.99
223_D243_R0.9330.99
10_E14_K0.9330.99
9_E13_E0.9320.99
40_D168_S0.9290.99
147_V150_A0.9270.99
107_D168_S0.9270.99
278_D281_Q0.9240.99
313_N327_L0.9220.99
79_E82_S0.9160.99
59_A63_A0.9150.99
12_Y45_V0.9140.99
143_W170_A0.9130.99
97_V100_I0.9080.98
25_V31_L0.9050.98
105_N281_Q0.9050.98
220_I248_P0.9020.98
43_E46_D0.8990.98
292_R295_A0.8980.98
173_K343_I0.8950.98
234_S262_I0.8940.98
246_H249_T0.8930.98
78_K82_S0.8930.98
257_D271_V0.8900.98
336_D340_E0.8890.98
335_L339_V0.8870.98
81_I85_Q0.8860.98
137_Y143_W0.8840.98
25_V28_P0.8770.98
12_Y42_Q0.8760.98
257_D263_K0.8760.98
72_E76_M0.8730.98
131_Y158_Y0.8720.98
210_E273_R0.8710.98
124_A155_T0.8710.98
70_D74_R0.8690.98
60_D64_M0.8690.98
9_E52_R0.8670.98
254_S271_V0.8670.98
109_N164_M0.8650.98
112_M174_L0.8580.98
148_M164_M0.8550.98
334_K337_E0.8540.98
244_L265_K0.8520.98
265_K269_M0.8440.98
247_L250_G0.8420.97
58_L88_T0.8400.97
12_Y41_I0.8400.97
57_Q84_L0.8350.97
150_A161_I0.8340.97
273_R277_Y0.8320.97
307_T314_R0.8290.97
335_L338_V0.8270.97
237_T242_V0.8240.97
217_E269_M0.8240.97
17_E20_S0.8240.97
184_Q307_T0.8220.97
309_N314_R0.8200.97
224_T230_P0.8200.97
84_L88_T0.8150.97
19_L38_Q0.8120.97
211_V273_R0.8100.97
108_K209_E0.8050.97
55_S59_A0.8020.97
136_R139_E0.8000.97
128_G153_T0.8000.97
116_G123_A0.7980.97
316_T323_T0.7980.97
12_Y16_N0.7980.97
279_K282_Q0.7970.96
54_A84_L0.7960.96
340_E344_V0.7950.96
255_C267_K0.7940.96
164_M270_K0.7910.96
263_K266_E0.7900.96
259_K270_K0.7890.96
128_G158_Y0.7840.96
18_L34_Y0.7810.96
253_V276_I0.7800.96
184_Q309_N0.7750.96
58_L84_L0.7690.96
69_L77_V0.7680.96
225_F256_Q0.7650.96
21_D26_N0.7640.96
50_Q53_D0.7600.95
82_S86_K0.7590.95
33_E37_E0.7550.95
213_V273_R0.7550.95
339_V343_I0.7540.95
201_T299_G0.7540.95
322_L330_I0.7530.95
276_I279_K0.7520.95
73_M76_M0.7510.95
222_V269_M0.7490.95
86_K89_E0.7470.95
320_I341_A0.7460.95
222_V242_V0.7450.95
64_M67_E0.7390.95
71_A75_D0.7370.95
116_G124_A0.7360.95
238_T260_S0.7330.94
51_Y54_A0.7320.94
245_T254_S0.7300.94
148_M271_V0.7290.94
16_N20_S0.7280.94
186_V192_G0.7280.94
135_S139_E0.7260.94
212_E273_R0.7250.94
230_P237_T0.7250.94
106_D175_K0.7240.94
118_A300_D0.7240.94
320_I342_L0.7230.94
315_V327_L0.7220.94
140_L336_D0.7220.94
10_E13_E0.7210.94
60_D67_E0.7150.94
58_L85_Q0.7150.94
318_H321_G0.7130.94
24_V30_K0.7100.93
208_A212_E0.7100.93
65_L81_I0.7090.93
24_V34_Y0.7080.93
124_A128_G0.7070.93
116_G151_N0.7070.93
328_D332_E0.7070.93
62_K66_E0.7060.93
129_N331_L0.7050.93
312_Q323_T0.7030.93
105_N277_Y0.7000.93
135_S161_I0.6990.93
97_V347_Q0.6990.93
49_R53_D0.6980.93
54_A58_L0.6970.93
226_A234_S0.6970.93
269_M273_R0.6930.93
37_E40_D0.6920.93
131_Y150_A0.6890.92
61_A65_L0.6890.92
24_V31_L0.6880.92
237_T254_S0.6870.92
131_Y145_T0.6870.92
312_Q316_T0.6870.92
223_D247_L0.6820.92
43_E47_V0.6820.92
67_E70_D0.6820.92
221_R254_S0.6800.92
199_T300_D0.6790.92
219_D248_P0.6780.92
228_S238_T0.6750.92
133_M335_L0.6740.92
70_D73_M0.6740.92
137_Y141_Q0.6730.92
320_I338_V0.6730.92
44_T98_L0.6720.91
98_L169_G0.6700.91
311_P314_R0.6700.91
235_V239_M0.6690.91
237_T262_I0.6690.91
41_I45_V0.6660.91
55_S88_T0.6620.91
213_V276_I0.6600.91
17_E21_D0.6590.91
35_S38_Q0.6570.91
149_E162_I0.6540.90
108_K208_A0.6510.90
154_G304_R0.6480.90
180_A295_A0.6480.90
325_Q329_Q0.6440.90
215_I244_L0.6430.90
9_E45_V0.6420.89
210_E270_K0.6420.89
39_S42_Q0.6420.89
56_E59_A0.6420.89
40_D102_K0.6380.89
14_K17_E0.6380.89
255_C271_V0.6370.89
314_R318_H0.6370.89
192_G196_H0.6350.89
249_T276_I0.6350.89
253_V271_V0.6330.89
244_L253_V0.6320.89
57_Q61_A0.6310.89
58_L62_K0.6310.89
72_E75_D0.6280.88
221_R269_M0.6270.88
172_S343_I0.6270.88
124_A159_K0.6250.88
263_K267_K0.6230.88
122_E196_H0.6220.88
290_Q293_K0.6220.88
115_R201_T0.6190.88
78_K85_Q0.6170.88
287_E291_T0.6160.87
10_E21_D0.6150.87
225_F241_A0.6150.87
19_L31_L0.6150.87
137_Y339_V0.6120.87
65_L73_M0.6110.87
230_P239_M0.6110.87
227_S241_A0.6100.87
63_A67_E0.6100.87
132_R136_R0.6100.87
288_Y292_R0.6090.87
338_V341_A0.6080.87
170_A174_L0.6080.87
121_E155_T0.6080.87
225_F245_T0.6070.87
324_I338_V0.6050.87
267_K271_V0.6050.87
110_V171_Y0.6040.86
208_A274_A0.6040.86
61_A77_V0.6030.86
34_Y38_Q0.6020.86
125_L310_F0.6010.86
114_I131_Y0.5980.86
247_L251_V0.5960.86
237_T263_K0.5950.86
180_A199_T0.5940.86
87_E91_L0.5930.85
151_N160_E0.5920.85
8_I44_T0.5900.85
253_V275_R0.5880.85
125_L128_G0.5860.85
166_T170_A0.5810.84
253_V258_E0.5810.84
132_R135_S0.5800.84
26_N31_L0.5800.84
136_R140_L0.5790.84
65_L74_R0.5790.84
128_G150_A0.5780.84
216_H219_D0.5770.84
178_N295_A0.5770.84
208_A281_Q0.5760.84
227_S254_S0.5750.84
208_A277_Y0.5740.84
128_G155_T0.5720.83
31_L38_Q0.5710.83
11_R15_L0.5710.83
30_K34_Y0.5700.83
191_S196_H0.5700.83
143_W173_K0.5680.83
313_N323_T0.5680.83
114_I200_A0.5680.83
33_E36_K0.5670.83
320_I323_T0.5660.83
180_A201_T0.5650.83
117_A199_T0.5630.82
58_L80_E0.5610.82
234_S260_S0.5610.82
225_F252_V0.5600.82
276_I280_F0.5600.82
116_G150_A0.5580.82
180_A303_E0.5570.82
175_K346_D0.5530.81
115_R203_A0.5490.81
224_T237_T0.5470.81
123_A180_A0.5450.80
222_V245_T0.5440.80
149_E160_E0.5420.80
230_P265_K0.5410.80
129_N183_V0.5400.80
237_T258_E0.5380.79
199_T303_E0.5350.79
118_A123_A0.5350.79
129_N188_E0.5320.79
205_L274_A0.5310.79
329_Q332_E0.5300.78
228_S254_S0.5280.78
203_A275_R0.5280.78
61_A64_M0.5280.78
83_E86_K0.5270.78
64_M77_V0.5260.78
54_A57_Q0.5260.78
59_A62_K0.5260.78
223_D254_S0.5250.78
309_N312_Q0.5250.78
38_Q41_I0.5220.77
32_R35_S0.5210.77
117_A180_A0.5210.77
313_N318_H0.5210.77
211_V281_Q0.5200.77
289_D292_R0.5170.77
33_E40_D0.5170.77
219_D247_L0.5170.77
239_M262_I0.5150.77
242_V253_V0.5140.76
228_S239_M0.5130.76
101_P175_K0.5120.76
47_V91_L0.5120.76
210_E266_E0.5110.76
84_L87_E0.5110.76
176_Y345_E0.5110.76
71_A74_R0.5100.76
252_V256_Q0.5090.76
221_R238_T0.5080.76
116_G159_K0.5080.76
36_K107_D0.5070.75
176_Y339_V0.5050.75
121_E125_L0.5050.75
296_V299_G0.5050.75
180_A296_V0.5040.75
40_D107_D0.5040.75
96_K344_V0.5040.75
234_S254_S0.5040.75
113_E194_R0.5030.75
70_D75_D0.5020.75
240_S262_I0.5020.75
81_I88_T0.5010.75
234_S257_D0.5010.75
226_A238_T0.5000.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1zbtA 1 0.8876 100 0.088 Contact Map
3d5aX 1 0.9916 100 0.092 Contact Map
2b3tB 1 0.8399 100 0.098 Contact Map
1rq0A 3 0.8933 100 0.153 Contact Map
2ihr1 1 0.9551 100 0.153 Contact Map
1gqeA 1 0.9607 100 0.161 Contact Map
2rsmA 1 0.3202 100 0.706 Contact Map
3j7yp 1 0.1629 100 0.742 Contact Map
1j26A 1 0.2444 100 0.79 Contact Map
4dh9Y 1 0.2669 99.9 0.805 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0112 seconds.