GREMLIN Database
PRPD - 2-methylcitrate dehydratase
UniProt: P45859 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 472 (434)
Sequences: 2714 (1869)
Seq/√Len: 89.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
176_A186_I5.4701.00
182_G185_E4.1551.00
308_R373_E3.9091.00
307_D401_K3.8831.00
67_K117_D3.8711.00
13_D183_R3.7801.00
132_T135_D3.3641.00
30_G456_E3.2141.00
415_L427_K3.1121.00
397_Q407_E3.0751.00
310_V375_T2.9491.00
292_A341_T2.7841.00
38_G432_F2.7741.00
307_D399_H2.7261.00
312_R377_N2.6971.00
310_V397_Q2.6451.00
31_H425_V2.6081.00
400_F406_T2.5821.00
305_E401_K2.5511.00
367_K371_K2.5481.00
139_M178_L2.5411.00
312_R395_A2.4911.00
308_R399_H2.4401.00
293_V344_G2.4401.00
27_T425_V2.4221.00
347_F354_H2.4221.00
189_A231_S2.4211.00
147_Q170_A2.3991.00
310_V373_E2.3951.00
224_G228_A2.3591.00
34_L449_Y2.3231.00
29_A141_I2.3121.00
399_H405_S2.2851.00
395_A410_E2.2701.00
21_T141_I2.2511.00
25_A271_E2.2441.00
179_L229_L2.2141.00
119_V131_L2.2011.00
433_S446_K2.1901.00
281_A285_A2.1501.00
115_A175_A2.1181.00
33_L140_M2.0941.00
112_I174_V2.0511.00
310_V399_H2.0431.00
115_A225_V2.0331.00
454_S457_T2.0191.00
112_I136_V2.0101.00
424_A427_K2.0081.00
138_E177_K2.0011.00
36_T88_I1.9971.00
429_L453_T1.9951.00
29_A137_L1.8981.00
312_R397_Q1.8871.00
349_D354_H1.8851.00
54_G233_K1.8751.00
22_S25_A1.8601.00
433_S445_H1.8561.00
172_T189_A1.8381.00
302_R305_E1.8161.00
311_I341_T1.7921.00
173_A190_L1.7881.00
18_K26_Y1.7591.00
24_E271_E1.7451.00
18_K134_R1.7411.00
156_L165_L1.7321.00
432_F436_L1.7231.00
446_K450_E1.7011.00
115_A179_L1.6911.00
312_R375_T1.6791.00
192_N212_R1.6791.00
88_I107_D1.6621.00
16_L183_R1.6541.00
347_F362_D1.6431.00
301_N400_F1.6331.00
109_L140_M1.6251.00
438_T441_P1.6211.00
400_F404_T1.6121.00
150_L169_V1.6061.00
34_L452_C1.6031.00
289_A344_G1.5821.00
14_Y268_Y1.5561.00
143_A174_V1.5461.00
179_L228_A1.5431.00
49_C87_M1.5361.00
194_W241_A1.5351.00
429_L449_Y1.5301.00
19_E134_R1.5181.00
433_S449_Y1.5171.00
347_F359_T1.5151.00
135_D138_E1.5121.00
26_Y30_G1.5111.00
41_I436_L1.5091.00
96_T274_L1.5061.00
397_Q405_S1.4951.00
202_T245_P1.4931.00
135_D177_K1.4901.00
169_V190_L1.4781.00
339_Y369_R1.4781.00
296_H348_G1.4650.99
66_S80_A1.4590.99
77_P81_A1.4590.99
133_V455_Y1.4480.99
291_S411_C1.4430.99
77_P118_Y1.4370.99
14_Y265_L1.4360.99
26_Y138_E1.4330.99
21_T26_Y1.4260.99
395_A407_E1.4230.99
346_L365_I1.4200.99
179_L232_L1.4170.99
68_V75_L1.4100.99
172_T224_G1.3970.99
367_K370_D1.3920.99
214_S334_D1.3920.99
115_A119_V1.3880.99
451_R458_L1.3850.99
290_E415_L1.3850.99
115_A178_L1.3810.99
450_E454_S1.3800.99
59_G233_K1.3790.99
40_G88_I1.3770.99
343_I354_H1.3740.99
222_S226_H1.3600.99
33_L137_L1.3590.99
22_S268_Y1.3470.99
442_D446_K1.3450.99
441_P445_H1.3260.99
15_V186_I1.3180.99
67_K74_V1.3150.99
20_I138_E1.3110.99
50_T54_G1.3030.99
192_N231_S1.3030.99
80_A84_I1.3020.99
346_L368_L1.2960.99
366_D369_R1.2900.99
176_A181_G1.2890.99
429_L433_S1.2840.99
112_I175_A1.2820.99
291_S396_V1.2790.99
12_T16_L1.2780.99
146_I173_A1.2780.99
282_E335_H1.2720.99
18_K21_T1.2450.98
12_T187_K1.2400.98
309_V398_V1.2380.98
57_V83_N1.2360.98
37_L452_C1.2320.98
31_H428_L1.2320.98
331_A334_D1.2310.98
460_T464_N1.2300.98
351_T354_H1.2290.98
13_D17_E1.2140.98
116_A136_V1.2110.98
285_A340_I1.2110.98
181_G231_S1.2070.98
289_A340_I1.2030.98
219_D222_S1.2020.98
135_D180_G1.1980.98
292_A398_V1.1930.98
281_A355_Y1.1930.98
8_I187_K1.1890.98
16_L186_I1.1880.98
32_V275_F1.1870.98
26_Y137_L1.1800.98
112_I140_M1.1690.97
304_D401_K1.1660.97
97_W101_E1.1650.97
15_V173_A1.1630.97
96_T283_F1.1600.97
426_P429_L1.1600.97
111_G171_T1.1460.97
230_M235_E1.1450.97
436_L445_H1.1410.97
304_D364_R1.1350.97
328_H332_D1.1230.97
64_N74_V1.1210.97
66_S117_D1.1180.97
28_T31_H1.1170.97
69_P113_L1.1130.97
9_E183_R1.1040.96
51_K211_S1.1040.96
136_V140_M1.1010.96
90_W214_S1.1000.96
448_I452_C1.1000.96
85_G216_A1.0990.96
322_D338_Q1.0950.96
295_L398_V1.0950.96
340_I355_Y1.0920.96
138_E142_K1.0850.96
131_L178_L1.0830.96
40_G69_P1.0790.96
76_D79_R1.0780.96
181_G189_A1.0770.96
97_W269_V1.0770.96
181_G185_E1.0730.96
49_C83_N1.0730.96
111_G175_A1.0700.96
162_D216_A1.0680.96
195_I216_A1.0630.96
175_A228_A1.0610.96
384_Y387_P1.0580.95
188_N236_M1.0570.95
89_R94_N1.0550.95
34_L429_L1.0540.95
9_E184_E1.0530.95
278_S283_F1.0470.95
289_A350_I1.0440.95
164_V199_A1.0440.95
106_S144_H1.0440.95
68_V83_N1.0380.95
219_D223_R1.0380.95
75_L83_N1.0340.95
171_T224_G1.0220.95
62_V65_G1.0150.94
413_F420_R1.0090.94
51_K235_E1.0080.94
306_I398_V1.0080.94
315_E381_T1.0080.94
52_L86_C1.0070.94
43_A87_M1.0050.94
181_G232_L1.0010.94
264_P267_A0.9990.94
82_F218_G0.9940.94
376_E381_T0.9940.94
279_Y290_E0.9930.94
69_P84_I0.9900.94
194_W200_L0.9890.94
430_E434_D0.9880.93
425_V453_T0.9850.93
292_A345_L0.9840.93
416_G422_E0.9830.93
106_S109_L0.9820.93
173_A186_I0.9790.93
9_E187_K0.9740.93
185_E188_N0.9720.93
92_D213_K0.9680.93
181_G228_A0.9660.93
269_V272_N0.9660.93
120_S464_N0.9630.93
114_A117_D0.9580.92
175_A224_G0.9560.92
26_Y141_I0.9560.92
35_D431_K0.9490.92
188_N231_S0.9470.92
61_T64_N0.9430.92
380_Y410_E0.9420.92
38_G435_N0.9390.92
168_K224_G0.9350.91
120_S131_L0.9340.91
274_L283_F0.9270.91
262_A265_L0.9260.91
337_L374_V0.9190.91
299_V406_T0.9150.91
195_I212_R0.9140.91
200_L252_V0.9130.90
275_F428_L0.9120.90
27_T31_H0.9120.90
324_K338_Q0.9070.90
79_R83_N0.9050.90
169_V194_W0.9010.90
82_F85_G0.9010.90
177_K186_I0.8980.90
156_L160_G0.8860.89
18_K137_L0.8830.89
81_A114_A0.8810.89
311_I372_M0.8770.89
86_C218_G0.8770.89
441_P444_Q0.8750.88
188_N237_G0.8740.88
118_Y229_L0.8730.88
131_L136_V0.8710.88
211_S235_E0.8710.88
283_F286_Q0.8700.88
306_I345_L0.8630.88
173_A176_A0.8620.88
278_S423_E0.8570.87
9_E239_P0.8490.87
89_R216_A0.8470.87
280_P350_I0.8470.87
119_V232_L0.8440.87
377_N380_Y0.8440.87
379_T382_E0.8420.87
440_F445_H0.8400.86
449_Y452_C0.8370.86
289_A292_A0.8370.86
450_E453_T0.8360.86
89_R334_D0.8330.86
66_S84_I0.8310.86
280_P354_H0.8300.86
327_L335_H0.8300.86
101_E151_A0.8290.86
159_V255_N0.8270.86
339_Y343_I0.8260.85
120_S460_T0.8240.85
229_L233_K0.8200.85
149_V264_P0.8200.85
342_A368_L0.8180.85
51_K54_G0.8180.85
77_P114_A0.8160.85
81_A111_G0.8120.84
15_V177_K0.8100.84
311_I396_V0.8070.84
78_V229_L0.8030.84
8_I191_S0.8020.84
202_T240_T0.8010.84
185_E232_L0.8010.84
288_A311_I0.8010.84
267_A272_N0.7990.84
14_Y145_E0.7980.83
172_T228_A0.7970.83
53_L71_T0.7950.83
444_Q447_H0.7950.83
9_E13_D0.7940.83
142_K177_K0.7940.83
84_I117_D0.7910.83
84_I114_A0.7900.83
311_I337_L0.7830.82
342_A372_M0.7820.82
43_A90_W0.7810.82
183_R187_K0.7810.82
98_L283_F0.7800.82
300_K348_G0.7790.82
436_L440_F0.7780.82
89_R162_D0.7780.82
288_A341_T0.7770.82
305_E402_D0.7760.82
299_V398_V0.7750.82
288_A337_L0.7750.82
284_H384_Y0.7730.81
26_Y134_R0.7690.81
96_T392_I0.7650.81
36_T109_L0.7600.80
292_A299_V0.7590.80
343_I365_I0.7590.80
15_V176_A0.7590.80
284_H314_H0.7560.80
198_A253_L0.7540.80
296_H345_L0.7530.80
94_N213_K0.7520.80
17_E183_R0.7500.79
168_K197_N0.7490.79
213_K216_A0.7460.79
459_Q465_E0.7450.79
303_I346_L0.7450.79
15_V190_L0.7440.79
296_H344_G0.7430.79
211_S236_M0.7430.79
85_G218_G0.7430.79
347_F364_R0.7420.79
111_G174_V0.7400.79
345_L368_L0.7400.79
347_F365_I0.7400.79
307_D371_K0.7380.78
315_E319_R0.7350.78
346_L364_R0.7350.78
18_K138_E0.7310.78
108_N143_A0.7310.78
364_R367_K0.7310.78
92_D335_H0.7300.78
75_L79_R0.7290.78
162_D213_K0.7290.78
97_W151_A0.7280.77
195_I200_L0.7280.77
279_Y415_L0.7270.77
29_A140_M0.7250.77
447_H451_R0.7240.77
61_T76_D0.7230.77
33_L109_L0.7210.77
237_G334_D0.7210.77
455_Y459_Q0.7190.77
27_T453_T0.7190.77
223_R227_L0.7190.77
58_P73_Y0.7160.76
42_L91_L0.7150.76
289_A293_V0.7140.76
89_R167_V0.7140.76
119_V178_L0.7140.76
23_A27_T0.7110.76
333_R360_A0.7100.76
343_I347_F0.7090.76
95_D106_S0.7040.75
314_H317_A0.7020.75
350_I355_Y0.6990.75
323_K332_D0.6990.75
152_L269_V0.6990.75
445_H448_I0.6990.75
292_A296_H0.6980.74
136_V463_V0.6970.74
415_L421_R0.6970.74
212_R216_A0.6920.74
229_L232_L0.6920.74
78_V222_S0.6920.74
344_G350_I0.6900.74
116_A119_V0.6900.74
65_G118_Y0.6890.73
156_L166_F0.6880.73
309_V368_L0.6840.73
204_R335_H0.6840.73
280_P435_N0.6820.73
48_E90_W0.6820.73
341_T345_L0.6800.72
325_G370_D0.6800.72
443_K447_H0.6800.72
436_L439_H0.6770.72
433_S437_K0.6760.72
27_T30_G0.6760.72
38_G41_I0.6710.71
181_G186_I0.6700.71
313_T374_V0.6690.71
91_L282_E0.6680.71
24_E27_T0.6650.71
53_L83_N0.6620.70
359_T362_D0.6610.70
427_K430_E0.6610.70
238_Y245_P0.6590.70
45_R50_T0.6590.70
296_H299_V0.6570.70
7_V243_S0.6570.70
371_K401_K0.6560.70
112_I143_A0.6560.70
97_W196_D0.6560.70
417_H420_R0.6560.70
112_I169_V0.6550.70
89_R217_A0.6550.70
460_T465_E0.6540.70
89_R107_D0.6530.69
33_L136_V0.6530.69
133_V136_V0.6520.69
162_D195_I0.6520.69
36_T40_G0.6520.69
288_A394_N0.6490.69
157_N204_R0.6470.69
303_I400_F0.6460.69
29_A270_M0.6460.69
149_V262_A0.6450.68
257_K260_K0.6440.68
433_S442_D0.6430.68
82_F222_S0.6420.68
42_L352_A0.6420.68
307_D310_V0.6410.68
284_H317_A0.6390.68
67_K84_I0.6380.68
52_L56_I0.6370.68
459_Q464_N0.6360.67
167_V199_A0.6350.67
131_L135_D0.6340.67
28_T32_V0.6330.67
105_P148_G0.6330.67
210_G214_S0.6320.67
246_G251_D0.6320.67
349_D430_E0.6310.67
318_I374_V0.6300.67
299_V400_F0.6290.67
292_A344_G0.6280.66
383_D391_S0.6270.66
12_T186_I0.6260.66
7_V259_I0.6250.66
283_F414_P0.6250.66
46_Y49_C0.6240.66
65_G77_P0.6240.66
391_S413_F0.6230.66
283_F417_H0.6210.66
445_H449_Y0.6200.65
93_Y280_P0.6190.65
164_V167_V0.6180.65
330_P360_A0.6170.65
112_I171_T0.6170.65
108_N112_I0.6170.65
150_L262_A0.6160.65
96_T103_G0.6160.65
451_R454_S0.6160.65
361_N366_D0.6160.65
282_E321_I0.6140.65
167_V220_A0.6130.65
133_V460_T0.6120.64
218_G221_T0.6110.64
33_L455_Y0.6110.64
173_A189_A0.6110.64
315_E376_E0.6100.64
141_I270_M0.6090.64
134_R446_K0.6080.64
180_G183_R0.6080.64
242_L250_Q0.6070.64
145_E149_V0.6070.64
34_L453_T0.6070.64
213_K334_D0.6050.64
31_H453_T0.6050.64
93_Y435_N0.6040.63
363_P378_K0.6030.63
12_T183_R0.6030.63
161_L253_L0.6010.63
134_R459_Q0.6010.63
20_I26_Y0.6010.63
164_V213_K0.6000.63
295_L299_V0.6000.63
92_D104_H0.6000.63
407_E410_E0.5990.63
39_C93_Y0.5970.63
435_N438_T0.5960.62
66_S77_P0.5950.62
284_H320_I0.5940.62
440_F444_Q0.5940.62
204_R214_S0.5930.62
446_K449_Y0.5920.62
21_T138_E0.5920.62
305_E404_T0.5910.62
161_L200_L0.5890.62
25_A28_T0.5880.61
97_W103_G0.5880.61
52_L83_N0.5880.61
170_A174_V0.5880.61
292_A396_V0.5870.61
116_A463_V0.5870.61
8_I242_L0.5870.61
57_V60_T0.5860.61
296_H303_I0.5860.61
250_Q259_I0.5850.61
89_R213_K0.5850.61
56_I60_T0.5850.61
115_A118_Y0.5850.61
10_E13_D0.5840.61
108_N174_V0.5830.61
18_K141_I0.5810.60
263_R398_V0.5800.60
290_E294_I0.5800.60
196_D227_L0.5790.60
458_L465_E0.5790.60
369_R372_M0.5790.60
201_R204_R0.5780.60
36_T110_G0.5780.60
32_V109_L0.5770.60
278_S290_E0.5760.60
378_K382_E0.5750.60
16_L142_K0.5750.60
300_K404_T0.5750.60
145_E268_Y0.5740.60
306_I309_V0.5740.60
209_T214_S0.5740.60
36_T106_S0.5730.59
367_K372_M0.5720.59
294_I298_Q0.5710.59
199_A216_A0.5690.59
298_Q406_T0.5690.59
105_P273_V0.5680.59
454_S458_L0.5680.59
160_G213_K0.5670.59
95_D213_K0.5660.58
411_C421_R0.5650.58
22_S271_E0.5650.58
20_I177_K0.5650.58
150_L242_L0.5650.58
36_T275_F0.5630.58
24_E418_R0.5630.58
182_G186_I0.5630.58
157_N164_V0.5630.58
331_A335_H0.5630.58
154_N166_F0.5610.58
165_L198_A0.5610.58
111_G115_A0.5610.58
168_K193_A0.5610.58
415_L424_A0.5580.57
157_N160_G0.5580.57
47_P51_K0.5580.57
36_T39_C0.5570.57
287_T394_N0.5570.57
49_C90_W0.5560.57
39_C92_D0.5560.57
94_N199_A0.5550.57
97_W148_G0.5540.57
89_R166_F0.5540.57
456_E465_E0.5540.57
15_V146_I0.5530.57
330_P357_A0.5530.57
144_H270_M0.5520.57
191_S195_I0.5520.57
373_E399_H0.5500.56
89_R199_A0.5500.56
400_F403_G0.5490.56
115_A229_L0.5490.56
188_N239_P0.5480.56
302_R400_F0.5480.56
88_I109_L0.5470.56
144_H273_V0.5470.56
353_Q438_T0.5440.56
159_V254_F0.5440.56
192_N227_L0.5410.55
97_W102_W0.5400.55
202_T247_W0.5400.55
324_K374_V0.5390.55
432_F448_I0.5390.55
134_R456_E0.5380.55
282_E417_H0.5370.55
447_H450_E0.5360.54
169_V172_T0.5360.54
31_H424_A0.5360.54
309_V372_M0.5350.54
458_L463_V0.5340.54
30_G452_C0.5330.54
35_D213_K0.5330.54
19_E22_S0.5330.54
339_Y356_E0.5320.54
98_L282_E0.5300.54
151_A201_R0.5290.53
213_K431_K0.5290.53
343_I350_I0.5290.53
401_K433_S0.5280.53
142_K173_A0.5280.53
330_P334_D0.5280.53
32_V273_V0.5270.53
156_L163_H0.5270.53
345_L400_F0.5240.53
163_H284_H0.5230.53
11_I190_L0.5220.53
152_L272_N0.5220.53
132_T459_Q0.5210.52
216_A334_D0.5200.52
298_Q447_H0.5190.52
7_V11_I0.5190.52
306_I400_F0.5170.52
280_P351_T0.5170.52
47_P50_T0.5150.52
134_R138_E0.5150.52
357_A360_A0.5150.52
57_V459_Q0.5140.51
285_A317_A0.5140.51
350_I354_H0.5140.51
303_I406_T0.5130.51
309_V341_T0.5120.51
202_T205_H0.5120.51
169_V193_A0.5110.51
304_D404_T0.5100.51
83_N114_A0.5090.51
60_T78_V0.5070.50
187_K239_P0.5070.50
7_V242_L0.5070.50
321_I335_H0.5070.50
116_A131_L0.5070.50
296_H301_N0.5070.50
89_R335_H0.5060.50
17_E142_K0.5060.50
196_D219_D0.5060.50
425_V429_L0.5060.50
32_V270_M0.5060.50
290_E423_E0.5050.50
437_K441_P0.5050.50
157_N331_A0.5040.50
303_I306_I0.5030.50
341_T396_V0.5030.50
60_T64_N0.5020.50
169_V198_A0.5000.49
451_R463_V0.5000.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1szqA 2 0.9894 100 0.112 Contact Map
2hp0A 2 0.9216 100 0.114 Contact Map
4rqoA 2 0.5318 97.2 0.937 Contact Map
4rgiA 1 0.1504 20.8 0.975 Contact Map
4bhxA 2 0.1102 18.4 0.976 Contact Map
4giuA 2 0.1017 16.9 0.976 Contact Map
1ti6A 3 0.1907 15.9 0.976 Contact Map
4e6sA 2 0.1102 13.1 0.977 Contact Map
1h0hA 1 0.2013 12.9 0.977 Contact Map
1bh9B 1 0.0996 12.3 0.977 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0126 seconds.