GREMLIN Database
CISY3 - 2-methylcitrate synthase
UniProt: P45858 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 372 (358)
Sequences: 3997 (2006)
Seq/√Len: 106.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
200_I213_G7.3961.00
197_A214_A3.9431.00
58_R64_E3.5501.00
62_E66_E3.3701.00
37_D40_E3.2391.00
159_A175_E3.1251.00
171_S286_K3.0501.00
298_A301_R3.0381.00
43_E278_E2.8831.00
156_S159_A2.8031.00
200_I217_S2.7061.00
61_E64_E2.6721.00
49_E65_M2.6641.00
281_Q297_E2.6521.00
250_K255_E2.6071.00
301_R305_K2.5801.00
192_N203_T2.5381.00
205_S219_K2.5381.00
242_D245_Q2.4991.00
235_L322_W2.4501.00
238_K242_D2.4351.00
174_E324_L2.4091.00
244_E305_K2.3691.00
244_E298_A2.3641.00
199_V357_F2.3161.00
103_L215_V2.2731.00
196_A217_S2.1591.00
160_N164_M2.1341.00
12_D355_R2.0891.00
125_A344_C2.0661.00
158_S179_D2.0001.00
278_E282_Q1.9991.00
203_T217_S1.9931.00
46_S65_M1.9891.00
51_V341_S1.9081.00
201_A210_A1.8661.00
244_E302_L1.8551.00
173_L286_K1.8401.00
155_L159_A1.8211.00
52_H58_R1.8201.00
84_R87_E1.8171.00
235_L328_I1.8051.00
296_C319_P1.8031.00
281_Q285_D1.7951.00
298_A302_L1.7851.00
141_R146_K1.7651.00
136_T337_S1.7631.00
156_S175_E1.7631.00
228_E232_Y1.7551.00
248_Q252_K1.7261.00
146_K149_I1.7241.00
245_Q249_T1.7111.00
101_T104_S1.6921.00
102_G135_L1.6851.00
176_Q179_D1.6821.00
117_S120_A1.6741.00
39_I274_L1.6711.00
326_I334_I1.6711.00
145_K149_I1.6641.00
240_T298_A1.6591.00
181_S321_Y1.6581.00
165_M326_I1.6371.00
80_A84_R1.6341.00
132_M340_T1.6341.00
201_A347_V1.6241.00
180_R279_A1.6191.00
100_R216_A1.6111.00
66_E70_R1.5821.00
248_Q306_E1.5781.00
100_R104_S1.5761.00
22_S187_E1.5751.00
171_S174_E1.5431.00
145_K148_P1.5421.00
30_Q37_D1.5271.00
213_G217_S1.5261.00
290_H322_W1.5231.00
152_L169_L1.5131.00
249_T254_K1.5041.00
249_T253_R1.5011.00
50_L183_V1.5011.00
55_L129_L1.4671.00
52_H64_E1.4451.00
63_S67_T1.4431.00
181_S276_M1.4401.00
98_G139_S1.4281.00
70_R74_S1.4151.00
172_S176_Q1.4091.00
137_A161_F1.4091.00
152_L163_Y1.4061.00
71_K126_Y1.4021.00
103_L132_M1.3921.00
48_L72_I1.3901.00
75_A127_Q1.3851.00
196_A213_G1.3781.00
147_E150_L1.3731.00
192_N217_S1.3731.00
149_I164_M1.3721.00
176_Q180_R1.3601.00
186_S341_S1.3521.00
83_L138_A1.3450.99
152_L155_L1.3400.99
12_D263_R1.3360.99
76_S157_Y1.3310.99
278_E281_Q1.3220.99
85_L105_A1.3220.99
151_P160_N1.3100.99
280_L319_P1.3020.99
249_T255_E1.2960.99
189_E360_R1.2760.99
196_A200_I1.2680.99
285_D290_H1.2580.99
151_P164_M1.2540.99
257_I303_M1.2540.99
145_K150_L1.2530.99
36_Y59_L1.2510.99
223_H226_A1.2460.99
54_L341_S1.2350.99
240_T301_R1.2310.99
143_I146_K1.2300.99
269_M274_L1.2270.99
146_K150_L1.2180.99
205_S209_G1.2170.99
260_F303_M1.2160.99
237_A250_K1.2080.99
34_R57_G1.2040.99
218_L336_F1.2030.99
76_S153_Q1.2030.99
209_G219_K1.2000.99
207_L344_C1.1990.99
277_K300_E1.1990.99
322_W328_I1.1960.99
239_T291_R1.1960.99
19_T22_S1.1950.99
84_R88_L1.1850.99
71_K123_E1.1740.99
118_P352_A1.1730.99
33_I186_S1.1710.99
18_E362_S1.1630.99
196_A199_V1.1610.98
241_S245_Q1.1540.98
39_I275_M1.1520.98
36_Y53_L1.1390.98
99_L136_T1.1300.98
96_M227_N1.1290.98
53_L59_L1.1230.98
279_A282_Q1.1190.98
124_R207_L1.1160.98
120_A124_R1.1130.98
222_L256_K1.1130.98
66_E69_E1.1100.98
247_L303_M1.1070.98
45_K49_E1.0960.98
46_S179_D1.0950.98
197_A347_V1.0930.98
67_T71_K1.0900.98
166_T174_E1.0880.98
52_H56_E1.0880.98
40_E44_T1.0870.98
277_K281_Q1.0820.98
55_L344_C1.0810.98
232_Y328_I1.0790.98
128_L211_L1.0750.98
239_T242_D1.0660.97
40_E43_E1.0650.97
173_L282_Q1.0640.97
23_Y26_T1.0600.97
302_L305_K1.0560.97
276_M320_V1.0540.97
207_L347_V1.0500.97
303_M306_E1.0480.97
78_L138_A1.0450.97
211_L340_T1.0360.97
211_L344_C1.0250.97
75_A126_Y1.0220.97
122_K348_I1.0200.97
205_S210_A1.0130.96
159_A172_S1.0090.96
233_L250_K1.0070.96
134_A151_P1.0020.96
179_D183_V0.9970.96
69_E73_N0.9950.96
72_I126_Y0.9950.96
26_T29_S0.9930.96
293_Y297_E0.9930.96
240_T295_M0.9910.96
338_A341_S0.9810.96
18_E364_M0.9790.96
47_Y341_S0.9780.96
198_R350_Q0.9750.96
58_R61_E0.9690.95
94_H228_E0.9620.95
9_P353_N0.9610.95
296_C318_A0.9580.95
56_E122_K0.9580.95
234_L299_G0.9560.95
237_A243_F0.9540.95
38_L275_M0.9500.95
251_L306_E0.9450.95
64_E67_T0.9440.95
188_H265_Y0.9420.95
123_E127_Q0.9410.95
246_L250_K0.9400.95
136_T333_P0.9390.95
247_L302_L0.9390.95
143_I329_P0.9380.95
33_I50_L0.9370.94
237_A246_L0.9330.94
244_E248_Q0.9300.94
348_I352_A0.9290.94
236_E255_E0.9260.94
140_Y144_N0.9210.94
243_F298_A0.9210.94
144_N148_P0.9090.94
226_A312_N0.9060.93
160_N163_Y0.9060.93
63_S66_E0.9050.93
36_Y41_L0.9050.93
20_H364_M0.9030.93
149_I163_Y0.9030.93
17_A190_M0.9020.93
290_H294_E0.8980.93
54_L186_S0.8960.93
22_S189_E0.8910.93
100_R212_T0.8910.93
86_L139_S0.8910.93
124_R208_Y0.8870.93
87_E147_E0.8850.93
185_Y313_L0.8850.93
89_L108_G0.8830.93
294_E297_E0.8790.92
52_H68_L0.8780.92
296_C315_Y0.8730.92
78_L135_L0.8710.92
143_I147_E0.8700.92
48_L158_S0.8690.92
36_Y58_R0.8680.92
229_A232_Y0.8660.92
176_Q279_A0.8660.92
235_L292_L0.8590.91
65_M69_E0.8570.91
155_L175_E0.8570.91
215_V336_F0.8560.91
134_A164_M0.8550.91
204_H222_L0.8540.91
277_K297_E0.8460.91
115_D120_A0.8460.91
155_L160_N0.8450.91
49_E59_L0.8420.91
86_L142_I0.8370.90
182_L337_S0.8360.90
297_E301_R0.8310.90
238_K291_R0.8280.90
224_G335_F0.8240.90
85_L89_L0.8220.90
193_S223_H0.8160.89
280_L296_C0.8150.89
190_M360_R0.8140.89
223_H314_D0.8140.89
260_F311_P0.8130.89
83_L87_E0.8130.89
240_T294_E0.8110.89
86_L102_G0.8080.89
158_S178_F0.8070.89
41_L50_L0.8060.89
227_N314_D0.8050.89
203_T356_L0.8050.89
195_F357_F0.8030.89
77_S150_L0.8020.88
245_Q248_Q0.7970.88
294_E298_A0.7960.88
218_L335_F0.7960.88
71_K75_A0.7960.88
87_E142_I0.7950.88
125_A348_I0.7950.88
121_N124_R0.7920.88
193_S224_G0.7900.88
46_S49_E0.7880.88
269_M277_K0.7880.88
176_Q282_Q0.7860.87
25_D28_S0.7820.87
158_S182_L0.7810.87
104_S216_A0.7810.87
143_I148_P0.7780.87
195_F199_V0.7770.87
41_L59_L0.7770.87
75_A123_E0.7740.87
218_L224_G0.7730.87
32_L368_Y0.7730.87
158_S175_E0.7730.87
55_L341_S0.7710.86
77_S153_Q0.7700.86
174_E283_L0.7690.86
129_L157_Y0.7670.86
23_Y363_Y0.7640.86
11_L14_V0.7630.86
210_A347_V0.7620.86
33_I53_L0.7620.86
238_K241_S0.7570.85
120_A123_E0.7550.85
223_H335_F0.7540.85
230_V234_L0.7520.85
189_E361_V0.7490.85
237_A299_G0.7490.85
23_Y28_S0.7470.85
227_N332_T0.7430.84
155_L170_P0.7430.84
45_K183_V0.7390.84
156_S179_D0.7380.84
85_L88_L0.7350.84
82_H105_A0.7300.83
16_A195_F0.7290.83
51_V68_L0.7270.83
42_S183_V0.7240.83
236_E250_K0.7240.83
279_A320_V0.7210.83
112_Q127_Q0.7200.82
82_H85_L0.7200.82
185_Y227_N0.7190.82
185_Y317_A0.7170.82
99_L215_V0.7160.82
173_L177_I0.7130.82
127_Q131_K0.7120.82
119_S123_E0.7050.81
51_V129_L0.7040.81
280_L320_V0.7040.81
234_L296_C0.7030.81
112_Q123_E0.7030.81
188_H224_G0.7020.81
82_H102_G0.7010.81
241_S244_E0.6970.80
14_V17_A0.6960.80
104_S212_T0.6960.80
281_Q293_Y0.6960.80
243_F302_L0.6940.80
143_I149_I0.6930.80
130_G133_P0.6910.80
336_F343_L0.6910.80
47_Y51_V0.6900.80
226_A261_G0.6890.80
231_M328_I0.6870.79
240_T244_E0.6870.79
231_M331_Y0.6860.79
32_L37_D0.6850.79
13_G16_A0.6800.79
141_R149_I0.6790.79
242_D249_T0.6780.79
155_L163_Y0.6770.78
276_M317_A0.6750.78
230_V295_M0.6750.78
292_L322_W0.6740.78
11_L195_F0.6730.78
200_I210_A0.6700.78
83_L150_L0.6700.78
26_T268_K0.6690.78
218_L227_N0.6690.78
233_L246_L0.6680.77
11_L15_I0.6660.77
170_P175_E0.6650.77
110_D127_Q0.6640.77
194_T332_T0.6620.77
250_K254_K0.6620.77
81_D109_Y0.6610.77
180_R183_V0.6590.77
181_S185_Y0.6580.76
193_S314_D0.6550.76
87_E138_A0.6540.76
38_L184_L0.6530.76
50_L186_S0.6530.76
17_A24_L0.6510.76
89_L105_A0.6500.76
230_V313_L0.6500.76
176_Q183_V0.6490.75
28_S268_K0.6490.75
289_D294_E0.6460.75
250_K253_R0.6450.75
229_A258_M0.6450.75
121_N125_A0.6430.75
194_T346_H0.6420.75
244_E247_L0.6380.74
81_D84_R0.6380.74
346_H354_N0.6380.74
17_A22_S0.6370.74
21_I34_R0.6360.74
204_H351_H0.6330.74
247_L295_M0.6320.74
75_A131_K0.6310.73
125_A207_L0.6310.73
47_Y179_D0.6310.73
294_E305_K0.6300.73
204_H221_N0.6300.73
138_A164_M0.6270.73
75_A214_A0.6230.72
67_T70_R0.6230.72
223_H261_G0.6230.72
232_Y235_L0.6220.72
45_K179_D0.6210.72
31_I38_L0.6210.72
280_L293_Y0.6180.72
13_G24_L0.6170.72
174_E287_A0.6160.72
261_G312_N0.6140.71
188_H335_F0.6120.71
179_D355_R0.6110.71
257_I307_K0.6110.71
81_D91_E0.6100.71
34_R51_V0.6070.70
291_R294_E0.6050.70
182_L338_A0.6050.70
283_L320_V0.6040.70
237_A247_L0.6030.70
112_Q115_D0.6000.70
188_H191_P0.6000.70
168_K174_E0.6000.70
32_L367_R0.5980.69
11_L16_A0.5960.69
77_S123_E0.5960.69
277_K316_Y0.5950.69
340_T344_C0.5950.69
189_E309_L0.5920.69
74_S153_Q0.5920.69
326_I330_L0.5920.69
89_L101_T0.5900.68
46_S69_E0.5890.68
73_N153_Q0.5890.68
206_D209_G0.5880.68
121_N351_H0.5870.68
144_N327_P0.5870.68
22_S32_L0.5860.68
134_A137_A0.5850.68
107_A206_D0.5850.68
36_Y40_E0.5840.68
248_Q251_L0.5840.68
206_D219_K0.5810.67
158_S162_L0.5810.67
85_L102_G0.5810.67
184_L275_M0.5760.67
132_M215_V0.5750.66
194_T198_R0.5750.66
185_Y314_D0.5750.66
82_H86_L0.5750.66
15_I22_S0.5740.66
137_A164_M0.5740.66
313_L317_A0.5740.66
193_S335_F0.5720.66
231_M322_W0.5720.66
304_E308_G0.5720.66
103_L128_L0.5720.66
13_G17_A0.5710.66
128_L207_L0.5700.66
244_E281_Q0.5690.66
287_A323_M0.5680.65
191_P313_L0.5670.65
181_S334_I0.5660.65
243_F295_M0.5650.65
78_L83_L0.5650.65
144_N147_E0.5630.65
11_L357_F0.5620.65
44_T49_E0.5600.64
188_H193_S0.5590.64
245_Q252_K0.5580.64
364_M367_R0.5580.64
256_K356_L0.5570.64
79_P106_L0.5550.64
198_R234_L0.5550.64
312_N318_A0.5540.64
79_P82_H0.5530.63
163_Y167_G0.5530.63
287_A290_H0.5520.63
89_L93_T0.5510.63
117_S121_N0.5500.63
106_L135_L0.5500.63
152_L160_N0.5500.63
71_K74_S0.5490.63
269_M310_Y0.5470.63
136_T215_V0.5470.63
309_L315_Y0.5460.62
113_I124_R0.5460.62
134_A160_N0.5450.62
162_L175_E0.5450.62
46_S51_V0.5440.62
110_D124_R0.5430.62
132_M136_T0.5400.62
177_I279_A0.5390.61
177_I283_L0.5380.61
51_V55_L0.5380.61
138_A142_I0.5380.61
110_D351_H0.5370.61
191_P262_H0.5320.60
191_P335_F0.5320.60
141_R145_K0.5320.60
51_V198_R0.5310.60
41_L53_L0.5300.60
138_A148_P0.5290.60
235_L331_Y0.5290.60
190_M198_R0.5290.60
198_R204_H0.5280.60
303_M315_Y0.5280.60
313_L320_V0.5270.60
233_L237_A0.5260.60
17_A21_I0.5250.59
19_T346_H0.5240.59
73_N76_S0.5240.59
281_Q301_R0.5240.59
288_G294_E0.5230.59
143_I150_L0.5230.59
48_L157_Y0.5230.59
347_V361_V0.5220.59
300_E304_E0.5210.59
25_D32_L0.5200.59
86_L148_P0.5190.59
121_N348_I0.5180.58
284_C293_Y0.5180.58
256_K296_C0.5180.58
92_D111_R0.5160.58
104_S107_A0.5150.58
318_A335_F0.5140.58
250_K307_K0.5120.58
201_A351_H0.5110.57
227_N331_Y0.5110.57
13_G22_S0.5100.57
193_S218_L0.5100.57
328_I331_Y0.5100.57
17_A364_M0.5090.57
18_E22_S0.5090.57
81_D85_L0.5080.57
133_P136_T0.5080.57
308_G311_P0.5080.57
46_S176_Q0.5060.57
29_S271_P0.5060.57
144_N149_I0.5060.57
180_R282_Q0.5050.57
280_L284_C0.5030.56
31_I35_G0.5030.56
118_P121_N0.5010.56
131_K134_A0.5000.56
45_K65_M0.5000.56
129_L341_S0.5000.56
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3hwkA 5 0.9785 100 0.047 Contact Map
2h12A 4 0.9973 100 0.055 Contact Map
1a59A 2 0.9892 100 0.056 Contact Map
4xghA 5 0.914 100 0.056 Contact Map
2ibpA 2 0.9946 100 0.057 Contact Map
1iomA 2 0.9812 100 0.06 Contact Map
3msuA 2 0.9892 100 0.062 Contact Map
1o7xA 2 0.9785 100 0.064 Contact Map
1vgpA 2 0.9758 100 0.068 Contact Map
1vgmA 2 0.9785 100 0.069 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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