GREMLIN Database
NUCB - Sporulation-specific extracellular nuclease
UniProt: P42983 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 136 (116)
Sequences: 118 (90)
Seq/√Len: 8.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
50_D104_R7.0311.00
118_V133_F4.4380.99
93_V122_M3.3110.93
89_W133_F2.6140.80
63_D66_G2.1680.66
60_C91_M2.1320.65
37_P60_C2.0760.63
50_D53_A2.0000.60
108_P114_A1.9320.57
48_I101_A1.8730.54
69_K84_Y1.8150.52
36_F117_W1.7600.50
103_V123_S1.7590.50
60_C94_C1.6890.47
37_P94_C1.6190.44
73_E76_K1.5470.41
68_D80_T1.5220.40
60_C89_W1.4940.39
103_V108_P1.4860.38
84_Y105_Y1.4840.38
44_T91_M1.4450.37
31_D132_L1.4190.36
107_T118_V1.3910.35
85_D112_R1.3650.34
44_T89_W1.3640.34
51_A55_G1.3570.33
117_W122_M1.3470.33
94_C117_W1.3200.32
36_F94_C1.3080.32
43_E107_T1.3020.31
20_V23_Q1.2860.31
79_P105_Y1.2840.31
42_P51_A1.2820.31
36_F118_V1.2570.30
46_S104_R1.2450.29
89_W117_W1.2450.29
49_R56_H1.2220.28
60_C117_W1.2060.28
22_Q27_A1.1930.28
34_L107_T1.1760.27
44_T114_A1.0960.24
37_P117_W1.0900.24
120_N123_S1.0900.24
52_I91_M1.0900.24
38_L45_G1.0860.24
29_S125_Y1.0660.23
81_K84_Y1.0640.23
39_S104_R1.0280.22
54_E104_R1.0270.22
49_R102_D1.0270.22
75_L85_D1.0230.22
75_L116_S1.0210.22
75_L112_R1.0150.22
42_P128_G1.0110.22
68_D72_E1.0100.22
53_A104_R1.0000.22
123_S131_V0.9970.21
107_T135_V0.9710.21
31_D59_I0.9700.21
38_L124_S0.9670.21
53_A77_G0.9640.21
35_Y99_A0.9620.20
42_P55_G0.9530.20
24_I29_S0.9470.20
22_Q76_K0.9280.20
53_A80_T0.9240.19
77_G130_R0.9150.19
49_R53_A0.9120.19
97_G113_G0.9110.19
23_Q50_D0.9110.19
53_A135_V0.9110.19
91_M117_W0.9090.19
57_P60_C0.9090.19
69_K73_E0.8960.19
30_Y59_I0.8910.19
37_P57_P0.8880.19
24_I27_A0.8870.19
33_V121_Q0.8780.18
95_E118_V0.8680.18
46_S95_E0.8600.18
89_W122_M0.8520.18
58_D132_L0.8390.17
26_G65_D0.8340.17
30_Y82_P0.8310.17
91_M101_A0.8250.17
96_E100_G0.8240.17
20_V25_Q0.8230.17
92_A128_G0.8220.17
86_R112_R0.8220.17
70_R119_G0.8190.17
49_R104_R0.8100.17
106_V132_L0.8090.17
107_T114_A0.8080.17
24_I59_I0.8050.17
30_Y132_L0.8010.16
43_E108_P0.7920.16
56_H132_L0.7910.16
31_D56_H0.7900.16
82_P114_A0.7860.16
40_R109_S0.7830.16
34_L46_S0.7820.16
99_A133_F0.7810.16
36_F41_Y0.7740.16
32_K103_V0.7620.16
70_R80_T0.7600.16
103_V124_S0.7470.15
64_R112_R0.7360.15
121_Q131_V0.7330.15
116_S129_T0.7330.15
82_P108_P0.7320.15
75_L86_R0.7310.15
61_T96_E0.7300.15
21_P25_Q0.7290.15
102_D107_T0.7230.15
44_T52_I0.7200.15
66_G125_Y0.7190.15
36_F116_S0.7170.15
76_K121_Q0.7130.14
46_S50_D0.7090.14
38_L106_V0.7060.14
81_K105_Y0.7060.14
53_A65_D0.7010.14
23_Q38_L0.6930.14
48_I93_V0.6790.14
46_S54_E0.6790.14
67_A100_G0.6730.14
21_P106_V0.6620.13
20_V24_I0.6590.13
49_R95_E0.6550.13
104_R109_S0.6530.13
41_Y122_M0.6520.13
91_M122_M0.6470.13
54_E77_G0.6460.13
61_T97_G0.6420.13
44_T60_C0.6410.13
23_Q69_K0.6330.13
36_F60_C0.6300.13
39_S117_W0.6260.13
31_D135_V0.6220.13
21_P24_I0.6170.13
75_L78_I0.6130.13
94_C118_V0.6110.13
32_K129_T0.6080.12
69_K121_Q0.6070.12
44_T81_K0.6050.12
36_F52_I0.6040.12
39_S94_C0.6040.12
120_N127_D0.6030.12
33_V132_L0.6030.12
52_I132_L0.6010.12
38_L58_D0.6010.12
37_P55_G0.5990.12
35_Y76_K0.5960.12
55_G112_R0.5830.12
61_T110_D0.5820.12
69_K109_S0.5770.12
43_E68_D0.5740.12
28_S126_P0.5600.12
43_E62_I0.5530.11
44_T110_D0.5500.11
44_T48_I0.5500.11
74_S129_T0.5490.11
40_R60_C0.5470.11
109_S126_P0.5460.11
71_R101_A0.5410.11
26_G59_I0.5360.11
60_C79_P0.5310.11
37_P91_M0.5270.11
20_V109_S0.5240.11
43_E106_V0.5230.11
71_R86_R0.5200.11
28_S38_L0.5130.11
82_P93_V0.5010.11
24_I104_R0.5000.11
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1fwxA 2 0.8971 15.5 0.956 Contact Map
1xt8A 1 0.6029 13.5 0.958 Contact Map
4od6A 1 0.5515 13.3 0.958 Contact Map
3do6A 3 0.9118 12.2 0.958 Contact Map
3zbiC 1 0.1691 11.4 0.959 Contact Map
4zdxA 1 0.5147 8.5 0.961 Contact Map
3cvzA 1 0.6029 8 0.962 Contact Map
4yv7A 1 0.7941 7.6 0.962 Contact Map
2qjyC 2 0.3971 7.4 0.962 Contact Map
1y0gA 2 0.7426 6.3 0.963 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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