GREMLIN Database
NASF - Uroporphyrinogen-III C-methyltransferase
UniProt: P42437 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 483 (370)
Sequences: 726 (580)
Seq/√Len: 30.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
11_V87_V5.5261.00
274_L279_A5.5201.00
26_K30_K4.6471.00
29_L35_I4.5881.00
214_G219_I4.1031.00
337_L353_F3.9251.00
54_Q79_K3.4611.00
202_Q210_R3.4441.00
357_A370_S3.4281.00
299_L322_A3.3671.00
106_A110_H3.2361.00
123_G174_Y3.1751.00
218_N222_K3.1391.00
126_A204_G3.0991.00
214_G218_N2.9881.00
79_K82_N2.9831.00
184_C220_Q2.9671.00
145_M174_Y2.9121.00
214_G222_K2.8851.00
186_Q189_S2.8751.00
19_G27_Q2.7841.00
212_V228_I2.6961.00
33_D50_S2.6131.00
39_R61_L2.5801.00
28_A117_V2.5631.00
25_G45_L2.5510.99
122_S174_Y2.5180.99
144_A168_V2.5000.99
194_P217_E2.4870.99
200_V212_V2.4680.99
34_V79_K2.4650.99
274_L281_L2.3910.99
347_E350_K2.3030.99
339_A358_I2.2310.99
95_S100_V2.2310.99
200_V228_I2.1680.98
77_V106_A2.1220.98
198_V219_I2.0680.98
54_Q84_L2.0500.98
96_V134_V2.0290.97
36_L76_L2.0200.97
264_G268_D1.9910.97
221_Q225_E1.9580.97
24_K27_Q1.9570.97
315_A340_A1.8990.96
129_Y247_W1.8780.96
67_M75_L1.8490.95
95_S116_M1.8380.95
80_A112_I1.8200.95
11_V117_V1.8110.95
126_A201_I1.8010.95
23_I26_K1.7900.94
24_K31_E1.7870.94
104_A245_H1.7770.94
349_E354_L1.7610.94
181_S224_Q1.7580.94
39_R62_P1.7440.94
74_A78_E1.7420.94
211_S243_Q1.7270.93
35_I53_C1.7150.93
146_I171_L1.7030.93
266_D314_R1.6990.93
222_K225_E1.6840.92
21_L45_L1.6800.92
162_E166_R1.6630.92
273_K277_S1.6350.91
81_L110_H1.6240.91
246_S249_E1.6070.91
96_V143_F1.6060.90
7_I113_R1.5960.90
317_C347_E1.5920.90
96_V145_M1.5760.90
257_H280_D1.5740.90
77_V110_H1.5510.89
27_Q31_E1.5410.89
44_K275_R1.5360.88
339_A355_V1.5010.87
310_F319_F1.4930.87
9_Y28_A1.4910.87
349_E355_V1.4880.87
29_L49_A1.4830.87
36_L75_L1.4750.86
262_T287_W1.4630.86
21_L26_K1.4610.86
127_P204_G1.4610.86
289_T314_R1.4600.86
23_I48_F1.4590.86
144_A167_S1.4420.85
78_E82_N1.4300.84
341_S344_A1.4210.84
105_D139_F1.4180.84
360_P370_S1.4090.84
184_C216_L1.3990.83
185_Q189_S1.3950.83
257_H282_I1.3940.83
21_L25_G1.3910.83
339_A345_K1.3900.83
96_V124_I1.3850.82
71_E74_A1.3740.82
204_G233_I1.3680.82
215_T218_N1.3670.82
127_P134_V1.3660.82
134_V172_V1.3640.82
195_S217_E1.3460.81
338_S367_V1.3380.80
74_A346_T1.3240.80
15_P205_T1.3030.78
223_V228_I1.3020.78
146_I173_F1.2960.78
192_K216_L1.2880.78
204_G247_W1.2810.77
173_F236_I1.2780.77
39_R230_N1.2780.77
56_I79_K1.2560.76
324_A349_E1.2490.75
174_Y233_I1.2430.75
348_L355_V1.2400.75
126_A247_W1.2270.74
102_E137_R1.2260.74
26_K48_F1.2150.73
200_V223_V1.1960.72
213_K240_V1.1960.72
95_S126_A1.1940.72
264_G283_E1.1740.71
262_T266_D1.1740.71
105_D138_D1.1640.70
62_P178_K1.1570.70
120_I124_I1.1430.69
27_Q30_K1.1420.68
307_D338_S1.1380.68
262_T285_P1.1360.68
24_K28_A1.1220.67
62_P230_N1.1200.67
31_E276_D1.1150.67
271_A283_E1.1140.67
122_S127_P1.1130.66
222_K226_H1.1120.66
192_K196_V1.1080.66
198_V234_I1.1060.66
200_V219_I1.1010.66
31_E85_T1.0970.65
327_K349_E1.0960.65
345_K358_I1.0950.65
268_D283_E1.0860.65
17_D129_Y1.0840.64
252_P277_S1.0810.64
144_A169_Q1.0760.64
270_L343_Q1.0750.64
95_S127_P1.0710.63
260_V267_E1.0670.63
35_I49_A1.0660.63
177_V200_V1.0620.63
230_N234_I1.0620.63
163_G166_R1.0560.62
240_V243_Q1.0510.62
126_A174_Y1.0500.62
129_Y287_W1.0480.62
184_C188_I1.0360.61
181_S220_Q1.0350.61
141_S150_D1.0280.60
223_V227_Q1.0280.60
328_I332_Q1.0230.60
7_I115_E1.0220.60
121_T124_I1.0200.60
261_V287_W1.0120.59
287_W305_F1.0110.59
132_I199_I1.0000.58
127_P247_W0.9980.58
238_D241_N0.9930.57
30_K48_F0.9910.57
364_K368_V0.9890.57
327_K354_L0.9890.57
363_E366_L0.9840.57
134_V204_G0.9800.56
143_F174_Y0.9780.56
320_F351_R0.9780.56
357_A366_L0.9780.56
180_L223_V0.9750.56
292_M322_A0.9750.56
127_P174_Y0.9740.56
292_M299_L0.9690.56
366_L370_S0.9580.55
8_V80_A0.9570.55
36_L79_K0.9560.55
295_N299_L0.9540.54
168_V192_K0.9500.54
41_A45_L0.9490.54
330_I353_F0.9460.54
25_G46_L0.9440.54
9_Y85_T0.9390.53
32_A87_V0.9360.53
219_I234_I0.9350.53
160_D163_G0.9350.53
146_I164_L0.9320.53
361_D366_L0.9310.53
327_K332_Q0.9290.52
8_V112_I0.9120.51
243_Q249_E0.9090.51
219_I222_K0.9080.51
90_K175_M0.9070.51
143_F172_V0.9060.51
214_G234_I0.9050.51
144_A192_K0.9000.50
322_A325_S0.8990.50
126_A233_I0.8960.50
146_I236_I0.8960.50
60_K69_Q0.8880.49
105_D109_E0.8830.49
139_F241_N0.8830.49
123_G145_M0.8780.48
67_M71_E0.8710.48
316_V344_A0.8700.48
100_V340_A0.8670.48
247_W287_W0.8650.47
307_D357_A0.8630.47
340_A364_K0.8620.47
196_V238_D0.8620.47
27_Q272_D0.8610.47
32_A285_P0.8600.47
317_C320_F0.8590.47
324_A330_I0.8560.47
90_K99_R0.8550.47
321_R325_S0.8540.46
39_R63_N0.8490.46
11_V25_G0.8470.46
292_M295_N0.8430.46
263_H268_D0.8420.46
223_V338_S0.8370.45
211_S249_E0.8360.45
292_M301_K0.8360.45
127_P235_V0.8360.45
177_V202_Q0.8350.45
160_D166_R0.8350.45
198_V216_L0.8340.45
338_S359_Q0.8280.44
177_V230_N0.8270.44
279_A353_F0.8260.44
53_C87_V0.8220.44
11_V29_L0.8180.44
161_W190_Y0.8170.44
69_Q90_K0.8160.44
71_E78_E0.8090.43
317_C321_R0.8080.43
23_I229_T0.8070.43
90_K97_F0.8030.42
330_I355_V0.8000.42
159_L236_I0.8000.42
25_G64_R0.7990.42
58_C72_I0.7970.42
50_S87_V0.7960.42
261_V341_S0.7940.42
310_F316_V0.7930.42
163_G192_K0.7910.42
99_R175_M0.7840.41
129_Y205_T0.7820.41
186_Q190_Y0.7800.41
313_R347_E0.7780.41
207_G249_E0.7770.40
295_N322_A0.7740.40
95_S128_L0.7740.40
40_L175_M0.7720.40
26_K29_L0.7720.40
176_G234_I0.7650.40
138_D141_S0.7640.39
177_V234_I0.7640.39
330_I337_L0.7630.39
271_A281_L0.7630.39
97_F175_M0.7590.39
101_G137_R0.7590.39
345_K349_E0.7580.39
312_S344_A0.7580.39
314_R341_S0.7570.39
69_Q175_M0.7550.39
201_I323_L0.7530.39
247_W285_P0.7530.39
140_A168_V0.7520.39
57_Y66_F0.7510.39
149_H177_V0.7500.38
9_Y319_F0.7500.38
21_L30_K0.7500.38
136_H139_F0.7490.38
341_S359_Q0.7490.38
198_V227_Q0.7470.38
205_T247_W0.7470.38
19_G24_K0.7460.38
95_S247_W0.7450.38
151_K157_P0.7430.38
212_V226_H0.7410.38
260_V266_D0.7390.38
243_Q246_S0.7380.38
283_E320_F0.7380.38
270_L277_S0.7360.37
28_A338_S0.7360.37
178_K257_H0.7340.37
258_L350_K0.7340.37
127_P201_I0.7310.37
73_N76_L0.7280.37
148_A183_I0.7280.37
194_P275_R0.7270.37
40_L69_Q0.7270.37
181_S221_Q0.7270.37
104_A116_M0.7240.36
83_G262_T0.7220.36
80_A110_H0.7190.36
261_V315_A0.7190.36
214_G217_E0.7180.36
249_E254_I0.7150.36
129_Y285_P0.7140.36
243_Q254_I0.7140.36
213_K243_Q0.7070.35
142_S148_A0.7050.35
40_L176_G0.7050.35
285_P302_I0.7040.35
74_A110_H0.7040.35
324_A327_K0.7010.35
125_A353_F0.7010.35
148_A179_N0.7000.35
35_I120_I0.7000.35
22_T120_I0.7000.35
309_F371_R0.6990.35
97_F135_T0.6980.35
324_A355_V0.6970.35
37_Y147_T0.6950.34
162_E190_Y0.6910.34
152_S157_P0.6910.34
126_A172_V0.6870.34
160_D332_Q0.6860.34
260_V271_A0.6860.34
344_A359_Q0.6810.33
40_L98_G0.6800.33
320_F330_I0.6800.33
68_K71_E0.6790.33
116_M128_L0.6790.33
236_I250_S0.6760.33
298_I301_K0.6740.33
149_H176_G0.6740.33
312_S359_Q0.6740.33
301_K322_A0.6710.33
172_V235_V0.6710.33
10_F100_V0.6690.33
218_N367_V0.6670.32
343_Q346_T0.6660.32
185_Q288_R0.6650.32
62_P356_T0.6630.32
10_F58_C0.6630.32
25_G29_L0.6630.32
146_I226_H0.6620.32
58_C338_S0.6600.32
130_A211_S0.6600.32
91_G137_R0.6590.32
15_P20_L0.6580.32
66_F75_L0.6580.32
40_L99_R0.6570.32
264_G343_Q0.6520.31
261_V362_S0.6510.31
220_Q234_I0.6490.31
198_V220_Q0.6480.31
265_E317_C0.6470.31
177_V223_V0.6460.31
181_S185_Q0.6410.31
303_G335_A0.6410.31
311_T341_S0.6390.30
40_L90_K0.6360.30
36_L335_A0.6350.30
50_S55_F0.6340.30
140_A165_A0.6340.30
21_L48_F0.6340.30
90_K98_G0.6310.30
98_G175_M0.6310.30
169_Q192_K0.6300.30
264_G287_W0.6280.30
129_Y249_E0.6270.30
324_A354_L0.6250.30
248_F254_I0.6250.30
42_N169_Q0.6230.29
247_W262_T0.6220.29
328_I337_L0.6190.29
60_K72_I0.6190.29
262_T312_S0.6180.29
253_L279_A0.6180.29
338_S356_T0.6170.29
24_K373_A0.6160.29
266_D313_R0.6150.29
260_V373_A0.6130.29
38_D97_F0.6130.29
25_G337_L0.6130.29
114_Y283_E0.6110.29
40_L97_F0.6090.28
79_K84_L0.6070.28
121_T174_Y0.6060.28
276_D304_T0.6050.28
203_W206_W0.6050.28
45_L203_W0.6030.28
297_E301_K0.6030.28
120_I138_D0.6000.28
177_V212_V0.5990.28
72_I175_M0.5990.28
60_K99_R0.5980.28
54_Q173_F0.5970.28
134_V233_I0.5940.28
196_V216_L0.5920.27
167_S192_K0.5910.27
19_G275_R0.5910.27
35_I148_A0.5900.27
43_P321_R0.5900.27
309_F368_V0.5890.27
130_A201_I0.5880.27
230_N334_T0.5860.27
322_A326_Q0.5850.27
141_S158_N0.5850.27
69_Q99_R0.5830.27
184_C258_L0.5820.27
307_D311_T0.5820.27
262_T305_F0.5800.27
111_G269_P0.5790.27
220_Q224_Q0.5780.27
198_V228_I0.5780.27
20_L129_Y0.5770.26
302_I322_A0.5760.26
64_R120_I0.5750.26
55_F58_C0.5740.26
61_L64_R0.5730.26
61_L284_W0.5720.26
15_P247_W0.5720.26
194_P296_E0.5710.26
59_G122_S0.5710.26
177_V285_P0.5710.26
126_A145_M0.5700.26
309_F375_E0.5680.26
60_K175_M0.5680.26
60_K90_K0.5670.26
261_V312_S0.5670.26
91_G136_H0.5660.26
5_N113_R0.5650.26
100_V137_R0.5650.26
40_L60_K0.5640.26
309_F338_S0.5630.26
192_K208_R0.5630.26
95_S245_H0.5620.26
340_A365_R0.5610.25
102_E148_A0.5610.25
258_L332_Q0.5600.25
289_T302_I0.5590.25
289_T318_E0.5580.25
11_V89_L0.5580.25
340_A358_I0.5580.25
306_E335_A0.5570.25
83_G258_L0.5570.25
321_R351_R0.5570.25
162_E224_Q0.5560.25
294_V322_A0.5550.25
90_K135_T0.5530.25
33_D85_T0.5520.25
62_P126_A0.5520.25
6_G200_V0.5520.25
77_V373_A0.5510.25
11_V48_F0.5500.25
30_K217_E0.5500.25
62_P284_W0.5490.25
266_D365_R0.5480.25
95_S104_A0.5480.25
351_R356_T0.5460.25
51_P55_F0.5450.25
39_R57_Y0.5450.25
259_M348_L0.5450.25
69_Q135_T0.5440.24
206_W342_E0.5440.24
69_Q98_G0.5440.24
159_L164_L0.5440.24
50_S140_A0.5430.24
219_I240_V0.5430.24
324_A328_I0.5410.24
114_Y148_A0.5410.24
312_S367_V0.5400.24
33_D49_A0.5380.24
283_E287_W0.5370.24
42_N122_S0.5350.24
171_L183_I0.5350.24
287_W341_S0.5350.24
303_G358_I0.5340.24
19_G116_M0.5340.24
53_C143_F0.5340.24
356_T359_Q0.5330.24
207_G330_I0.5330.24
43_P308_V0.5330.24
199_I240_V0.5330.24
182_Y185_Q0.5320.24
60_K98_G0.5310.24
19_G269_P0.5310.24
336_K356_T0.5290.24
29_L87_V0.5280.24
311_T343_Q0.5280.24
129_Y209_Q0.5280.24
173_F183_I0.5280.24
324_A351_R0.5270.23
240_V266_D0.5260.23
148_A177_V0.5240.23
144_A177_V0.5240.23
197_P215_T0.5230.23
144_A163_G0.5220.23
271_A275_R0.5220.23
5_N81_L0.5210.23
110_H114_Y0.5210.23
72_I99_R0.5210.23
347_E351_R0.5200.23
185_Q346_T0.5200.23
34_V105_D0.5180.23
324_A347_E0.5170.23
290_E293_P0.5160.23
338_S364_K0.5160.23
106_A238_D0.5160.23
213_K244_T0.5160.23
303_G332_Q0.5160.23
40_L72_I0.5160.23
266_D287_W0.5150.23
184_C271_A0.5150.23
60_K65_H0.5140.23
258_L289_T0.5140.23
38_D175_M0.5140.23
75_L79_K0.5140.23
36_L66_F0.5130.23
312_S341_S0.5130.23
72_I90_K0.5130.23
272_D350_K0.5130.23
90_K147_T0.5130.23
112_I184_C0.5120.23
69_Q97_F0.5120.23
114_Y129_Y0.5110.23
134_V371_R0.5110.23
172_V309_F0.5100.23
45_L76_L0.5100.23
65_H91_G0.5090.22
22_T216_L0.5090.22
59_G239_I0.5080.22
39_R145_M0.5080.22
142_S168_V0.5080.22
62_P161_W0.5070.22
42_N270_L0.5060.22
135_T175_M0.5060.22
287_W312_S0.5060.22
56_I238_D0.5060.22
204_G220_Q0.5060.22
212_V223_V0.5060.22
76_L98_G0.5060.22
223_V284_W0.5050.22
38_D90_K0.5040.22
339_A348_L0.5030.22
198_V214_G0.5030.22
140_A167_S0.5030.22
33_D48_F0.5030.22
69_Q231_P0.5030.22
91_G147_T0.5020.22
95_S368_V0.5000.22
114_Y246_S0.5000.22
366_L369_G0.5000.22
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1pjqA 2 0.5052 100 0.649 Contact Map
1s4dA 4 0.5197 100 0.655 Contact Map
2yboA 2 0.4969 100 0.661 Contact Map
4e16A 2 0.472 100 0.692 Contact Map
1cbfA 2 0.4865 100 0.692 Contact Map
1ve2A 2 0.47 100 0.696 Contact Map
1va0A 2 0.4824 100 0.699 Contact Map
3ndcA 2 0.4928 100 0.709 Contact Map
2zvbA 2 0.4472 100 0.727 Contact Map
1wyzA 2 0.4513 100 0.728 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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