YXJG - BACSU - GREMLIN Contacts

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YXJG - Uncharacterized protein YxjG

UniProt:	P42318	Sequences: MSQQTTPAEQKSLQRKKPPFRADQVGSLLRSEPVKKARLQKAAGEMTAEQLRQIENDEIIRIVEKQKETGLNVVTDGEFRRAWWHFDFLENLDGVEPFTPEQGIQFHNVQTKARGIKVTGDIDFSTHPMLEDYSFLHSIAGDATPKMTIPSPNMLFFRGKLEKDEYKNDYQLFQHDVSKAYKKAIQAFYDRGCRYLQLDDTAWAVFLSEKGLKQIEAFGTTPDELRQLFAKSINDAIADRPDDLTVTMHICRGNFQSTWTAEGGYDAAAETIFDGLNLDGLFLEYDDSRSGGFEPLRYVKRSDLQLVLGLVTSKFGELENPDDVKRRIEEASRYVSLDQLCLSPQCGFASTEEGNKLTEEQQWAKLRHVVEIANDVWK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	378 (317)
Sequences:	4931 (2870)
Seq/√Len:	161.2

ECOLI - METE
BACSU - YXJG YXJH METE
HALSA - Q9HQH4
SULSO - METE Q980B0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
47_T	50_Q	4.954	1.00
319_E	324_V	3.503	1.00
40_Q	45_E	3.448	1.00
89_L	155_L	3.402	1.00
64_E	68_E	3.151	1.00
65_K	363_W	3.069	1.00
325_K	375_D	2.953	1.00
58_E	61_R	2.945	1.00
60_I	64_E	2.859	1.00
133_Y	159_G	2.820	1.00
321_P	371_E	2.745	1.00
36_K	40_Q	2.708	1.00
76_D	145_P	2.563	1.00
41_K	48_A	2.518	1.00
48_A	52_R	2.512	1.00
85_H	150_P	2.509	1.00
328_I	342_L	2.492	1.00
321_P	375_D	2.484	1.00
165_E	172_L	2.362	1.00
63_V	145_P	2.355	1.00
93_D	123_D	2.349	1.00
320_N	323_D	2.322	1.00
182_K	239_D	2.295	1.00
53_Q	57_D	2.187	1.00
234_N	237_I	2.142	1.00
37_A	54_I	2.141	1.00
50_Q	53_Q	2.122	1.00
52_R	131_E	2.076	1.00
317_E	323_D	2.053	1.00
186_Q	190_D	2.044	1.00
185_I	196_L	2.009	1.00
92_L	180_A	2.003	1.00
57_D	61_R	1.968	1.00
233_I	236_A	1.944	1.00
144_T	194_R	1.927	1.00
324_V	372_I	1.920	1.00
287_D	290_S	1.903	1.00
124_F	187_A	1.899	1.00
370_V	374_N	1.890	1.00
255_F	272_I	1.844	1.00
80_R	87_D	1.815	1.00
300_K	304_L	1.812	1.00
150_P	154_M	1.809	1.00
64_E	67_K	1.809	1.00
230_A	234_N	1.762	1.00
33_P	57_D	1.758	1.00
25_V	344_P	1.754	1.00
32_E	36_K	1.725	1.00
270_E	298_Y	1.708	1.00
55_E	81_R	1.707	1.00
41_K	101_E	1.698	1.00
95_V	155_L	1.687	1.00
360_E	364_A	1.666	1.00
122_I	180_A	1.661	1.00
198_L	237_I	1.655	1.00
63_V	139_I	1.647	1.00
46_M	50_Q	1.630	1.00
231_K	235_D	1.611	1.00
28_L	363_W	1.611	1.00
336_S	339_Q	1.609	1.00
229_F	233_I	1.590	1.00
325_K	329_E	1.582	1.00
84_W	150_P	1.570	1.00
344_P	369_V	1.561	1.00
227_Q	231_K	1.548	1.00
33_P	58_E	1.544	1.00
230_A	233_I	1.544	1.00
332_S	340_L	1.527	1.00
60_I	135_F	1.502	1.00
131_E	134_S	1.500	1.00
281_L	304_L	1.499	1.00
235_D	238_A	1.493	1.00
335_V	339_Q	1.469	1.00
175_H	232_S	1.467	1.00
133_Y	193_C	1.467	1.00
73_V	146_K	1.453	1.00
57_D	60_I	1.452	1.00
308_L	331_A	1.449	1.00
233_I	237_I	1.446	1.00
175_H	235_D	1.445	1.00
185_I	198_L	1.438	1.00
93_D	125_S	1.430	1.00
65_K	68_E	1.421	1.00
40_Q	46_M	1.407	1.00
187_A	190_D	1.396	1.00
311_V	319_E	1.382	1.00
322_D	326_R	1.374	1.00
96_E	117_K	1.367	1.00
321_P	372_I	1.362	1.00
321_P	368_H	1.360	1.00
297_R	334_Y	1.359	1.00
58_E	62_I	1.358	1.00
37_A	46_M	1.350	1.00
335_V	340_L	1.346	1.00
60_I	139_I	1.336	1.00
121_D	176_D	1.335	1.00
194_R	245_T	1.333	1.00
361_Q	364_A	1.332	1.00
195_Y	247_T	1.329	1.00
329_E	333_R	1.323	1.00
53_Q	56_N	1.317	1.00
69_T	367_R	1.316	1.00
234_N	277_N	1.306	1.00
67_K	143_A	1.303	1.00
176_D	179_K	1.281	1.00
325_K	372_I	1.279	1.00
247_T	280_G	1.268	1.00
299_V	304_L	1.247	1.00
117_K	160_K	1.244	1.00
170_Y	175_H	1.241	1.00
363_W	367_R	1.241	1.00
106_F	116_I	1.240	1.00
52_R	132_D	1.236	1.00
321_P	325_K	1.235	1.00
28_L	65_K	1.234	1.00
25_V	75_T	1.227	1.00
68_E	367_R	1.217	1.00
149_I	184_A	1.210	1.00
305_Q	341_C	1.208	1.00
91_N	128_P	1.206	1.00
39_L	43_A	1.205	1.00
116_I	129_M	1.202	1.00
88_F	92_L	1.197	1.00
54_I	57_D	1.195	1.00
187_A	191_R	1.189	1.00
251_C	346_C	1.187	1.00
371_E	374_N	1.186	1.00
173_F	228_L	1.164	1.00
49_E	53_Q	1.147	1.00
186_Q	240_R	1.145	1.00
364_A	367_R	1.138	1.00
272_I	281_L	1.136	1.00
52_R	81_R	1.134	1.00
311_V	369_V	1.131	1.00
61_R	65_K	1.123	1.00
28_L	62_I	1.122	1.00
307_V	343_S	1.119	1.00
342_L	369_V	1.106	1.00
94_G	155_L	1.099	1.00
71_L	366_L	1.084	0.99
307_V	341_C	1.076	0.99
154_M	202_A	1.061	0.99
201_T	276_L	1.059	0.99
89_L	116_I	1.052	0.99
280_G	305_Q	1.052	0.99
52_R	56_N	1.052	0.99
310_L	328_I	1.046	0.99
95_V	106_F	1.046	0.99
311_V	324_V	1.032	0.99
123_D	287_D	1.024	0.99
123_D	183_K	1.024	0.99
75_T	148_T	1.023	0.99
135_F	139_I	1.018	0.99
96_E	119_T	1.018	0.99
181_Y	201_T	1.014	0.99
65_K	69_T	1.012	0.99
184_A	188_F	1.011	0.99
327_R	330_E	1.009	0.99
332_S	337_L	1.005	0.99
367_R	371_E	0.999	0.99
182_K	186_Q	0.998	0.99
180_A	183_K	0.993	0.99
196_L	244_L	0.991	0.99
186_Q	239_D	0.989	0.99
292_G	295_P	0.988	0.99
148_T	199_D	0.981	0.99
238_A	277_N	0.978	0.99
290_S	327_R	0.977	0.99
230_A	236_A	0.974	0.99
204_A	250_I	0.966	0.99
24_Q	146_K	0.963	0.99
249_H	282_F	0.961	0.99
310_L	324_V	0.961	0.99
151_S	200_D	0.956	0.99
41_K	46_M	0.955	0.99
329_E	332_S	0.955	0.99
54_I	58_E	0.946	0.99
161_L	165_E	0.940	0.99
173_F	232_S	0.937	0.98
331_A	340_L	0.936	0.98
34_V	79_F	0.935	0.98
189_Y	241_P	0.934	0.98
92_L	184_A	0.934	0.98
189_Y	244_L	0.933	0.98
124_F	183_K	0.930	0.98
248_M	278_L	0.928	0.98
178_S	182_K	0.920	0.98
248_M	272_I	0.920	0.98
153_N	207_L	0.906	0.98
330_E	333_R	0.900	0.98
156_F	229_F	0.899	0.98
229_F	234_N	0.898	0.98
56_N	80_R	0.892	0.98
156_F	173_F	0.891	0.98
98_F	102_Q	0.890	0.98
232_S	236_A	0.886	0.98
63_V	136_L	0.885	0.98
157_F	202_A	0.883	0.98
135_F	138_S	0.878	0.98
133_Y	172_L	0.876	0.98
237_I	240_R	0.874	0.98
228_L	233_I	0.870	0.98
82_A	112_K	0.870	0.98
322_D	325_K	0.868	0.98
163_K	166_Y	0.866	0.97
227_Q	271_T	0.857	0.97
327_R	331_A	0.856	0.97
159_G	162_E	0.856	0.97
172_L	176_D	0.856	0.97
62_I	79_F	0.854	0.97
72_N	373_A	0.852	0.97
34_V	55_E	0.852	0.97
256_Q	295_P	0.852	0.97
170_Y	231_K	0.851	0.97
202_A	205_V	0.850	0.97
184_A	187_A	0.848	0.97
154_M	201_T	0.846	0.97
48_A	101_E	0.839	0.97
86_F	114_R	0.839	0.97
371_E	375_D	0.839	0.97
26_G	249_H	0.838	0.97
287_D	312_T	0.835	0.97
182_K	236_A	0.833	0.97
25_V	366_L	0.830	0.97
323_D	326_R	0.829	0.97
157_F	205_V	0.829	0.97
193_C	196_L	0.828	0.97
178_S	236_A	0.827	0.97
146_K	341_C	0.822	0.97
37_A	55_E	0.822	0.97
161_L	169_D	0.815	0.96
74_V	143_A	0.813	0.96
178_S	235_D	0.813	0.96
66_Q	366_L	0.812	0.96
50_Q	54_I	0.808	0.96
231_K	275_G	0.807	0.96
175_H	231_K	0.807	0.96
285_Y	308_L	0.806	0.96
227_Q	275_G	0.804	0.96
169_D	173_F	0.803	0.96
179_K	235_D	0.802	0.96
291_G	294_E	0.801	0.96
100_P	103_G	0.799	0.96
324_V	327_R	0.797	0.96
48_A	98_F	0.796	0.96
194_R	243_D	0.794	0.96
92_L	177_V	0.790	0.96
270_E	275_G	0.789	0.96
288_S	291_G	0.786	0.96
268_A	272_I	0.783	0.95
70_G	143_A	0.781	0.95
294_E	297_R	0.776	0.95
186_Q	189_Y	0.775	0.95
73_V	341_C	0.775	0.95
292_G	296_L	0.769	0.95
189_Y	243_D	0.768	0.95
113_A	116_I	0.765	0.95
56_N	131_E	0.761	0.95
179_K	183_K	0.761	0.95
170_Y	228_L	0.759	0.95
62_I	65_K	0.757	0.95
299_V	306_L	0.753	0.94
118_V	122_I	0.753	0.94
56_N	59_I	0.751	0.94
369_V	373_A	0.748	0.94
203_W	248_M	0.742	0.94
134_S	137_H	0.741	0.94
183_K	186_Q	0.739	0.94
318_L	358_T	0.735	0.94
126_T	131_E	0.734	0.94
175_H	179_K	0.732	0.94
89_L	98_F	0.730	0.93
25_V	343_S	0.727	0.93
109_V	112_K	0.727	0.93
146_K	197_Q	0.726	0.93
127_H	187_A	0.723	0.93
73_V	195_Y	0.723	0.93
28_L	366_L	0.721	0.93
297_R	330_E	0.720	0.93
157_F	169_D	0.720	0.93
237_I	241_P	0.719	0.93
51_L	55_E	0.719	0.93
368_H	372_I	0.712	0.93
232_S	237_I	0.711	0.93
154_M	157_F	0.710	0.92
300_K	339_Q	0.705	0.92
69_T	370_V	0.705	0.92
138_S	176_D	0.704	0.92
367_R	370_V	0.701	0.92
102_Q	105_Q	0.697	0.92
277_N	304_L	0.693	0.92
60_I	63_V	0.686	0.91
281_L	306_L	0.683	0.91
285_Y	292_G	0.682	0.91
68_E	363_W	0.682	0.91
130_L	184_A	0.682	0.91
29_L	362_Q	0.680	0.91
62_I	66_Q	0.677	0.91
293_F	330_E	0.676	0.91
312_T	315_F	0.675	0.90
157_F	208_S	0.674	0.90
80_R	103_G	0.674	0.90
161_L	172_L	0.672	0.90
40_Q	50_Q	0.672	0.90
130_L	191_R	0.668	0.90
250_I	255_F	0.668	0.90
254_N	291_G	0.668	0.90
46_M	54_I	0.666	0.90
234_N	238_A	0.665	0.90
344_P	366_L	0.663	0.90
88_F	91_N	0.661	0.90
120_G	125_S	0.660	0.89
61_R	359_E	0.659	0.89
55_E	59_I	0.658	0.89
324_V	342_L	0.655	0.89
358_T	361_Q	0.652	0.89
89_L	159_G	0.652	0.89
77_G	145_P	0.650	0.89
93_D	126_T	0.650	0.89
166_Y	171_Q	0.649	0.89
240_R	279_D	0.646	0.88
39_L	42_A	0.643	0.88
311_V	365_K	0.641	0.88
133_Y	137_H	0.639	0.88
297_R	333_R	0.639	0.88
202_A	230_A	0.636	0.88
269_A	299_V	0.635	0.88
157_F	207_L	0.630	0.87
188_F	191_R	0.629	0.87
245_T	279_D	0.629	0.87
199_D	346_C	0.629	0.87
71_L	370_V	0.627	0.87
157_F	201_T	0.627	0.87
241_P	244_L	0.626	0.87
67_K	140_A	0.626	0.87
153_N	202_A	0.625	0.87
71_L	74_V	0.624	0.87
110_Q	113_A	0.621	0.86
269_A	295_P	0.620	0.86
166_Y	172_L	0.620	0.86
239_D	242_D	0.619	0.86
74_V	145_P	0.619	0.86
146_K	195_Y	0.618	0.86
282_F	307_V	0.618	0.86
75_T	147_M	0.617	0.86
34_V	54_I	0.617	0.86
271_T	275_G	0.616	0.86
280_G	341_C	0.612	0.86
161_L	166_Y	0.612	0.86
252_R	347_G	0.610	0.85
358_T	362_Q	0.609	0.85
89_L	94_G	0.607	0.85
358_T	364_A	0.607	0.85
70_G	370_V	0.605	0.85
282_F	343_S	0.603	0.85
56_N	135_F	0.602	0.85
52_R	55_E	0.601	0.85
254_N	267_A	0.601	0.85
229_F	236_A	0.600	0.85
94_G	133_Y	0.597	0.84
171_Q	175_H	0.597	0.84
165_E	171_Q	0.597	0.84
86_F	104_I	0.596	0.84
90_E	98_F	0.593	0.84
195_Y	245_T	0.591	0.84
152_P	198_L	0.591	0.84
24_Q	73_V	0.591	0.84
34_V	58_E	0.590	0.84
99_T	104_I	0.590	0.84
69_T	363_W	0.586	0.83
100_P	104_I	0.583	0.83
35_K	39_L	0.582	0.83
23_D	373_A	0.580	0.83
366_L	370_V	0.580	0.83
285_Y	331_A	0.579	0.82
195_Y	280_G	0.578	0.82
39_L	317_E	0.577	0.82
250_I	281_L	0.576	0.82
245_T	305_Q	0.576	0.82
146_K	199_D	0.575	0.82
305_Q	339_Q	0.574	0.82
203_W	250_I	0.574	0.82
24_Q	71_L	0.573	0.82
153_N	173_F	0.572	0.82
199_D	249_H	0.571	0.82
328_I	372_I	0.569	0.81
336_S	340_L	0.569	0.81
21_R	341_C	0.569	0.81
105_Q	110_Q	0.567	0.81
175_H	178_S	0.566	0.81
155_L	159_G	0.565	0.81
85_H	88_F	0.562	0.81
49_E	52_R	0.562	0.81
291_G	331_A	0.560	0.80
251_C	284_E	0.559	0.80
24_Q	28_L	0.557	0.80
69_T	366_L	0.556	0.80
296_L	331_A	0.556	0.80
78_E	81_R	0.555	0.80
295_P	298_Y	0.555	0.80
270_E	274_D	0.555	0.80
226_R	271_T	0.555	0.80
36_K	39_L	0.553	0.79
316_G	356_K	0.553	0.79
32_E	39_L	0.553	0.79
56_N	60_I	0.551	0.79
99_T	102_Q	0.551	0.79
102_Q	106_F	0.550	0.79
153_N	229_F	0.549	0.79
28_L	362_Q	0.548	0.79
70_G	374_N	0.545	0.78
149_I	198_L	0.544	0.78
75_T	146_K	0.542	0.78
103_G	107_H	0.542	0.78
182_K	238_A	0.541	0.78
188_F	196_L	0.540	0.78
22_A	370_V	0.539	0.78
301_R	304_L	0.537	0.77
318_L	322_D	0.537	0.77
153_N	233_I	0.536	0.77
90_E	97_P	0.536	0.77
293_F	296_L	0.535	0.77
151_S	154_M	0.535	0.77
226_R	230_A	0.535	0.77
98_F	113_A	0.534	0.77
104_I	107_H	0.534	0.77
326_R	330_E	0.533	0.77
24_Q	366_L	0.533	0.77
97_P	134_S	0.533	0.77
55_E	80_R	0.533	0.77
40_Q	54_I	0.532	0.77
229_F	232_S	0.531	0.77
63_V	67_K	0.531	0.77
119_T	123_D	0.531	0.77
88_F	150_P	0.530	0.77
85_H	155_L	0.530	0.77
106_F	110_Q	0.530	0.77
63_V	74_V	0.530	0.77
124_F	184_A	0.529	0.76
320_N	372_I	0.529	0.76
127_H	130_L	0.528	0.76
324_V	369_V	0.527	0.76
136_L	139_I	0.526	0.76
129_M	132_D	0.526	0.76
31_S	58_E	0.525	0.76
65_K	359_E	0.524	0.76
42_A	86_F	0.523	0.76
162_E	165_E	0.523	0.76
255_F	283_L	0.522	0.75
254_N	295_P	0.521	0.75
90_E	103_G	0.521	0.75
204_A	253_G	0.520	0.75
41_K	51_L	0.520	0.75
236_A	239_D	0.520	0.75
203_W	253_G	0.520	0.75
94_G	139_I	0.519	0.75
124_F	130_L	0.519	0.75
88_F	184_A	0.518	0.75
119_T	160_K	0.518	0.75
293_F	327_R	0.517	0.75
34_V	39_L	0.517	0.75
172_L	177_V	0.515	0.75
274_D	338_D	0.515	0.75
33_P	61_R	0.513	0.74
124_F	127_H	0.512	0.74
104_I	158_R	0.512	0.74
189_Y	196_L	0.510	0.74
81_R	84_W	0.509	0.74
251_C	347_G	0.507	0.73
136_L	140_A	0.506	0.73
83_W	86_F	0.505	0.73
137_H	142_D	0.505	0.73
274_D	304_L	0.502	0.73
81_R	283_L	0.501	0.73
177_V	205_V	0.500	0.72
166_Y	170_Y	0.500	0.72

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1ypxA	1	0.8333	100	0.233	Contact Map
1t7lA	1	0.9127	100	0.302	Contact Map
2nq5A	1	0.8201	100	0.304	Contact Map
4l6hA	1	0.8995	100	0.311	Contact Map
1u1jA	1	0.8757	100	0.313	Contact Map
3rpdA	1	0.8757	100	0.324	Contact Map
1j93A	2	0.836	99.8	0.798	Contact Map
2ejaA	2	0.8333	99.8	0.802	Contact Map
4exqA	2	0.8466	99.8	0.804	Contact Map
4ay7A	1	0.8386	99.8	0.81	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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