EABN2 - BACSU - GREMLIN Contacts

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EABN2 - Extracellular endo-alpha-(1->5)-L-arabinanase 2

UniProt:	P42293	Sequences: MFNRLFRVCFLAALIMAFTLPNSVYAQKPIFKEVSVHDPSIIETNGTFYVFGSHLASAKSNDLMQWQQLTTSVSNDNPLIPNVYEELKETFEWAQSDTLWAADVTQLADGKYYMYYNACRGDSPRSAMGVAVADNIEGPYKNKGIFLKSGMEGTSSDGTPYDATKHPNVVDPHTFFDKDGKLWMVYGSYSGGIFILEMNPKTGFPLPGQGYGKKLLGGNHSRIEGPYVLYNPDTQYYYLYLSYGGLDATGGYNIRVARSKKPDGPYYDAEGNPMLDVRGKGGTFFDDRSIEPYGVKLMGSYTFETENEKGTGYVSPGHNSAYYDEKTGRSYLIFHTRFPGRGEEHEVRVHQLFMNKDGWPVAAPYRYAGETLKEVKQKDITGTYKLIQHGKDISADIKQTINIQLNKNHTISGEMTGTWRKTGKNTADITLAGKKYNGVFLRQWDSVREKNVMTFSVLNTSGEAVWGSK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	469 (359)
Sequences:	1838 (1382)
Seq/√Len:	72.9

ECOLI - YAGH
BACSU - EABN2 XYNB XYND EABN1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
181_K	197_E	8.021	1.00
47_T	59_K	7.490	1.00
236_Y	260_K	6.312	1.00
62_D	65_Q	5.794	1.00
111_K	134_D	5.552	1.00
133_A	139_P	5.166	1.00
178_K	230_Y	3.983	1.00
231_N	234_T	3.821	1.00
262_P	266_Y	3.622	1.00
46_G	61_N	3.426	1.00
47_T	61_N	3.378	1.00
321_A	352_L	3.140	1.00
236_Y	258_R	3.116	1.00
197_E	206_L	2.915	1.00
324_D	329_R	2.824	1.00
258_R	359_W	2.766	1.00
51_F	104_V	2.690	1.00
228_V	266_Y	2.690	1.00
111_K	132_V	2.665	1.00
199_N	203_G	2.636	1.00
175_F	179_D	2.420	1.00
51_F	140_Y	2.364	1.00
59_K	137_E	2.352	1.00
403_I	407_K	2.314	1.00
72_S	114_M	2.312	1.00
49_Y	137_E	2.286	1.00
195_I	263_D	2.246	1.00
59_K	67_Q	2.133	1.00
55_L	114_M	2.118	1.00
234_T	358_G	2.041	1.00
396_D	399_Q	1.992	1.00
329_R	351_Q	1.988	1.00
43_E	46_G	1.987	1.00
48_F	324_D	1.978	1.00
261_K	266_Y	1.965	1.00
199_N	204_F	1.948	1.00
354_M	360_P	1.943	1.00
175_F	228_V	1.931	1.00
59_K	69_L	1.916	1.00
323_Y	328_G	1.900	1.00
240_Y	256_V	1.878	1.00
44_T	112_Y	1.866	1.00
36_V	58_A	1.850	1.00
133_A	138_G	1.836	1.00
51_F	136_I	1.819	1.00
73_V	99_L	1.818	1.00
104_V	114_M	1.815	1.00
21_P	25_Y	1.811	1.00
213_K	263_D	1.792	1.00
400_T	403_I	1.754	1.00
183_W	263_D	1.748	1.00
394_S	397_I	1.708	1.00
22_N	26_A	1.707	1.00
260_K	267_Y	1.706	1.00
406_N	410_T	1.672	1.00
178_K	235_Q	1.655	1.00
353_F	356_K	1.638	1.00
324_D	327_T	1.638	1.00
395_A	399_Q	1.637	1.00
199_N	202_T	1.625	0.99
42_I	112_Y	1.622	0.99
259_S	265_P	1.604	0.99
394_S	399_Q	1.603	0.99
41_I	333_I	1.595	0.99
195_I	262_P	1.586	0.99
60_S	63_L	1.584	0.99
31_F	333_I	1.571	0.99
176_F	182_L	1.560	0.99
185_V	228_V	1.543	0.99
395_A	401_I	1.525	0.99
432_A	437_N	1.495	0.99
432_A	435_K	1.488	0.99
130_V	203_G	1.477	0.99
259_S	263_D	1.474	0.99
135_N	140_Y	1.458	0.99
323_Y	330_S	1.429	0.99
259_S	266_Y	1.424	0.99
193_I	241_L	1.413	0.99
70_T	139_P	1.402	0.98
401_I	407_K	1.400	0.98
196_L	205_P	1.397	0.98
229_L	321_A	1.381	0.98
175_F	237_Y	1.373	0.98
99_L	116_Y	1.372	0.98
215_L	218_G	1.370	0.98
186_Y	196_L	1.350	0.98
399_Q	403_I	1.349	0.98
195_I	228_V	1.342	0.98
106_Q	110_G	1.341	0.98
115_Y	202_T	1.333	0.98
181_K	206_L	1.325	0.98
21_P	24_V	1.323	0.98
177_D	230_Y	1.319	0.98
117_N	128_M	1.317	0.98
316_P	336_T	1.315	0.98
258_R	267_Y	1.313	0.98
213_K	216_L	1.310	0.98
195_I	215_L	1.289	0.97
43_E	325_E	1.284	0.97
396_D	403_I	1.281	0.97
438_G	443_Q	1.271	0.97
55_L	116_Y	1.264	0.97
395_A	403_I	1.259	0.97
435_K	438_G	1.253	0.97
431_L	436_Y	1.252	0.97
401_I	404_Q	1.250	0.97
332_L	362_A	1.240	0.97
230_Y	237_Y	1.234	0.97
334_F	350_H	1.230	0.96
177_D	237_Y	1.230	0.96
207_P	212_G	1.224	0.96
72_S	131_A	1.224	0.96
256_V	360_P	1.224	0.96
236_Y	359_W	1.221	0.96
19_T	22_N	1.221	0.96
117_N	172_P	1.211	0.96
198_M	203_G	1.206	0.96
46_G	112_Y	1.201	0.96
238_Y	256_V	1.200	0.96
56_A	71_T	1.198	0.96
435_K	439_V	1.190	0.96
231_N	358_G	1.183	0.96
352_L	362_A	1.179	0.96
52_G	225_G	1.167	0.95
17_A	20_L	1.155	0.95
223_I	241_L	1.132	0.94
132_V	143_K	1.128	0.94
259_S	264_G	1.128	0.94
243_Y	255_R	1.124	0.94
407_K	410_T	1.118	0.94
150_G	169_V	1.114	0.94
425_N	429_I	1.100	0.93
195_I	266_Y	1.082	0.93
397_I	402_N	1.075	0.93
331_Y	351_Q	1.071	0.93
51_F	72_S	1.063	0.92
258_R	354_M	1.047	0.92
109_D	180_G	1.045	0.92
55_L	131_A	1.044	0.92
37_H	345_H	1.041	0.91
438_G	444_W	1.039	0.91
114_M	131_A	1.029	0.91
185_V	226_P	1.025	0.91
116_Y	129_G	1.023	0.91
40_S	318_H	1.019	0.91
212_G	216_L	1.014	0.90
231_N	354_M	1.012	0.90
103_D	173_H	1.002	0.90
236_Y	259_S	1.002	0.90
394_S	403_I	0.994	0.89
22_N	25_Y	0.994	0.89
107_L	182_L	0.991	0.89
36_V	52_G	0.986	0.89
94_A	97_D	0.983	0.89
105_T	176_F	0.982	0.89
179_D	230_Y	0.982	0.89
397_I	401_I	0.980	0.89
102_A	114_M	0.974	0.88
62_D	66_W	0.973	0.88
187_G	190_S	0.971	0.88
335_H	338_F	0.969	0.88
55_L	99_L	0.968	0.88
373_K	384_Y	0.966	0.88
399_Q	407_K	0.965	0.88
430_T	433_G	0.962	0.88
68_Q	71_T	0.959	0.88
227_Y	320_S	0.954	0.87
55_L	102_A	0.951	0.87
235_Q	260_K	0.951	0.87
27_Q	331_Y	0.949	0.87
115_Y	184_M	0.948	0.87
399_Q	404_Q	0.940	0.87
135_N	139_P	0.935	0.86
211_Y	215_L	0.934	0.86
52_G	58_A	0.933	0.86
26_A	29_P	0.927	0.86
33_E	346_E	0.921	0.86
188_S	194_F	0.918	0.85
38_D	101_A	0.914	0.85
443_Q	446_S	0.910	0.85
145_I	148_K	0.910	0.85
256_V	321_A	0.907	0.85
173_H	185_V	0.907	0.85
403_I	411_I	0.907	0.85
44_T	106_Q	0.900	0.84
240_Y	254_I	0.900	0.84
216_L	219_N	0.896	0.84
170_V	226_P	0.891	0.84
252_Y	317_G	0.890	0.84
170_V	244_G	0.889	0.83
39_P	50_V	0.886	0.83
18_F	22_N	0.886	0.83
18_F	24_V	0.882	0.83
18_F	21_P	0.880	0.83
445_D	448_R	0.878	0.83
402_N	407_K	0.878	0.83
262_P	265_P	0.875	0.82
118_A	126_S	0.874	0.82
230_Y	235_Q	0.868	0.82
386_L	389_H	0.866	0.82
395_A	407_K	0.863	0.82
331_Y	349_V	0.858	0.81
215_L	219_N	0.857	0.81
69_L	137_E	0.853	0.81
435_K	441_L	0.848	0.80
66_W	237_Y	0.847	0.80
436_Y	440_F	0.846	0.80
231_N	330_S	0.844	0.80
429_I	438_G	0.842	0.80
20_L	24_V	0.840	0.80
226_P	239_L	0.832	0.79
105_T	174_T	0.828	0.79
431_L	438_G	0.826	0.79
399_Q	402_N	0.826	0.79
57_S	104_V	0.823	0.78
185_V	266_Y	0.822	0.78
433_G	436_Y	0.819	0.78
373_K	377_Q	0.818	0.78
423_G	446_S	0.816	0.78
196_L	207_P	0.815	0.78
397_I	400_T	0.814	0.78
317_G	324_D	0.814	0.78
191_G	220_H	0.813	0.78
275_L	362_A	0.812	0.78
228_V	262_P	0.812	0.78
386_L	390_G	0.811	0.77
187_G	191_G	0.810	0.77
51_F	102_A	0.805	0.77
109_D	204_F	0.802	0.77
427_A	440_F	0.801	0.77
38_D	318_H	0.800	0.76
372_L	375_V	0.798	0.76
395_A	400_T	0.797	0.76
43_E	322_Y	0.797	0.76
358_G	361_V	0.796	0.76
38_D	345_H	0.795	0.76
226_P	241_L	0.795	0.76
18_F	25_Y	0.795	0.76
371_T	374_E	0.794	0.76
44_T	47_T	0.793	0.76
63_L	320_S	0.792	0.76
174_T	198_M	0.790	0.76
236_Y	267_Y	0.789	0.75
53_S	245_G	0.786	0.75
70_T	74_S	0.785	0.75
254_I	257_A	0.784	0.75
53_S	252_Y	0.781	0.75
213_K	217_G	0.780	0.75
57_S	72_S	0.780	0.75
396_D	407_K	0.778	0.74
325_E	329_R	0.778	0.74
107_L	113_Y	0.774	0.74
198_M	201_K	0.771	0.74
417_G	421_K	0.770	0.74
394_S	401_I	0.763	0.73
17_A	21_P	0.759	0.73
71_T	74_S	0.759	0.73
339_P	342_G	0.759	0.73
231_N	323_Y	0.754	0.72
241_L	257_A	0.754	0.72
113_Y	130_V	0.754	0.72
317_G	335_H	0.753	0.72
170_V	252_Y	0.751	0.72
48_F	60_S	0.750	0.72
46_G	325_E	0.749	0.72
405_L	410_T	0.749	0.72
254_I	319_N	0.748	0.72
31_F	41_I	0.747	0.71
43_E	324_D	0.737	0.70
193_I	226_P	0.735	0.70
421_K	424_K	0.734	0.70
411_I	431_L	0.733	0.70
242_S	317_G	0.733	0.70
40_S	227_Y	0.732	0.70
43_E	323_Y	0.731	0.70
254_I	332_L	0.726	0.69
432_A	438_G	0.725	0.69
246_L	252_Y	0.725	0.69
118_A	127_A	0.721	0.69
423_G	427_A	0.721	0.69
374_E	392_D	0.719	0.69
324_D	328_G	0.719	0.69
323_Y	329_R	0.718	0.68
407_K	414_E	0.715	0.68
38_D	53_S	0.715	0.68
131_A	143_K	0.712	0.68
189_Y	194_F	0.712	0.68
60_S	225_G	0.711	0.68
238_Y	258_R	0.709	0.67
315_S	337_R	0.707	0.67
380_I	384_Y	0.707	0.67
27_Q	64_M	0.706	0.67
436_Y	442_R	0.705	0.67
434_K	437_N	0.704	0.67
375_V	384_Y	0.704	0.67
273_P	276_D	0.701	0.67
131_A	140_Y	0.700	0.66
434_K	441_L	0.692	0.66
425_N	440_F	0.692	0.66
397_I	405_L	0.689	0.65
409_H	412_S	0.688	0.65
416_T	420_R	0.687	0.65
59_K	68_Q	0.685	0.65
17_A	22_N	0.685	0.65
136_I	140_Y	0.683	0.65
106_Q	112_Y	0.682	0.64
90_T	93_W	0.682	0.64
101_A	318_H	0.682	0.64
230_Y	234_T	0.682	0.64
30_I	345_H	0.680	0.64
378_K	412_S	0.680	0.64
133_A	137_E	0.680	0.64
133_A	140_Y	0.680	0.64
427_A	431_L	0.678	0.64
398_K	402_N	0.677	0.64
403_I	406_N	0.676	0.64
261_K	265_P	0.676	0.64
259_S	267_Y	0.675	0.64
334_F	348_R	0.675	0.64
148_K	169_V	0.675	0.64
146_F	149_S	0.674	0.64
135_N	138_G	0.674	0.64
89_E	92_E	0.673	0.63
216_L	257_A	0.673	0.63
242_S	252_Y	0.672	0.63
335_H	346_E	0.672	0.63
442_R	448_R	0.671	0.63
51_F	114_M	0.671	0.63
149_S	169_V	0.671	0.63
214_K	217_G	0.671	0.63
377_Q	382_G	0.671	0.63
231_N	236_Y	0.669	0.63
57_S	110_G	0.668	0.63
32_K	104_V	0.667	0.63
424_K	427_A	0.667	0.63
433_G	437_N	0.667	0.63
403_I	409_H	0.666	0.63
394_S	398_K	0.665	0.62
183_W	197_E	0.664	0.62
116_Y	142_N	0.664	0.62
197_E	207_P	0.663	0.62
365_Y	369_G	0.662	0.62
115_Y	172_P	0.662	0.62
242_S	254_I	0.661	0.62
425_N	442_R	0.659	0.62
252_Y	335_H	0.657	0.62
193_I	223_I	0.656	0.61
372_L	434_K	0.656	0.61
117_N	170_V	0.655	0.61
144_G	203_G	0.654	0.61
352_L	360_P	0.653	0.61
364_P	367_Y	0.651	0.61
73_V	131_A	0.650	0.61
374_E	378_K	0.647	0.60
401_I	410_T	0.647	0.60
41_I	320_S	0.646	0.60
321_A	330_S	0.643	0.60
243_Y	246_L	0.641	0.60
313_Y	336_T	0.640	0.60
45_N	59_K	0.639	0.59
53_S	315_S	0.639	0.59
112_Y	135_N	0.639	0.59
313_Y	317_G	0.638	0.59
42_I	135_N	0.637	0.59
377_Q	392_D	0.635	0.59
318_H	335_H	0.634	0.59
45_N	141_K	0.634	0.59
414_E	417_G	0.634	0.59
429_I	433_G	0.634	0.59
115_Y	128_M	0.633	0.59
363_A	368_A	0.631	0.58
404_Q	407_K	0.630	0.58
55_L	170_V	0.630	0.58
133_A	141_K	0.630	0.58
48_F	61_N	0.629	0.58
433_G	439_V	0.628	0.58
42_I	136_I	0.628	0.58
401_I	405_L	0.627	0.58
66_W	264_G	0.626	0.58
336_T	348_R	0.626	0.58
399_Q	416_T	0.625	0.58
220_H	223_I	0.624	0.58
60_S	66_W	0.624	0.58
174_T	184_M	0.623	0.57
275_L	278_R	0.623	0.57
70_T	137_E	0.622	0.57
410_T	429_I	0.620	0.57
97_D	119_C	0.618	0.57
128_M	184_M	0.617	0.57
22_N	28_K	0.617	0.57
324_D	331_Y	0.616	0.57
174_T	182_L	0.615	0.57
51_F	57_S	0.615	0.57
438_G	441_L	0.615	0.57
59_K	136_I	0.615	0.57
181_K	260_K	0.615	0.57
330_S	360_P	0.614	0.56
199_N	206_L	0.614	0.56
413_G	417_G	0.614	0.56
50_V	347_V	0.613	0.56
396_D	409_H	0.612	0.56
437_N	442_R	0.611	0.56
99_L	102_A	0.610	0.56
399_Q	406_N	0.610	0.56
142_N	145_I	0.609	0.56
193_I	266_Y	0.609	0.56
132_V	203_G	0.609	0.56
252_Y	318_H	0.609	0.56
173_H	227_Y	0.608	0.56
36_V	40_S	0.608	0.56
417_G	420_R	0.607	0.56
55_L	73_V	0.605	0.55
423_G	429_I	0.604	0.55
425_N	428_D	0.603	0.55
101_A	172_P	0.602	0.55
225_G	319_N	0.601	0.55
185_V	195_I	0.600	0.55
128_M	170_V	0.599	0.55
330_S	354_M	0.598	0.54
182_L	198_M	0.598	0.54
89_E	94_A	0.596	0.54
400_T	404_Q	0.596	0.54
316_P	350_H	0.595	0.54
21_P	26_A	0.594	0.54
93_W	97_D	0.593	0.54
109_D	113_Y	0.592	0.54
441_L	445_D	0.590	0.53
396_D	402_N	0.590	0.53
399_Q	410_T	0.590	0.53
134_D	393_I	0.589	0.53
27_Q	30_I	0.588	0.53
49_Y	59_K	0.588	0.53
119_C	128_M	0.587	0.53
442_R	445_D	0.586	0.53
110_G	179_D	0.585	0.53
256_V	274_M	0.584	0.53
413_G	418_T	0.584	0.53
53_S	101_A	0.584	0.53
400_T	407_K	0.583	0.53
267_Y	359_W	0.582	0.52
390_G	393_I	0.582	0.52
20_L	23_S	0.582	0.52
375_V	381_T	0.581	0.52
313_Y	348_R	0.581	0.52
91_F	94_A	0.580	0.52
49_Y	138_G	0.579	0.52
367_Y	370_E	0.579	0.52
399_Q	409_H	0.578	0.52
256_V	269_A	0.575	0.52
53_S	317_G	0.573	0.51
244_G	317_G	0.572	0.51
101_A	345_H	0.570	0.51
19_T	26_A	0.569	0.51
440_F	445_D	0.569	0.51
226_P	244_G	0.569	0.51
135_N	376_K	0.568	0.51
357_D	425_N	0.564	0.50
19_T	24_V	0.562	0.50
48_F	325_E	0.562	0.50
436_Y	444_W	0.562	0.50
44_T	136_I	0.561	0.50
443_Q	447_V	0.561	0.50
396_D	401_I	0.558	0.49
433_G	440_F	0.558	0.49
331_Y	399_Q	0.557	0.49
231_N	321_A	0.557	0.49
99_L	114_M	0.556	0.49
102_A	106_Q	0.552	0.49
53_S	345_H	0.551	0.49
113_Y	132_V	0.551	0.49
329_R	371_T	0.550	0.48
115_Y	174_T	0.550	0.48
90_T	95_Q	0.548	0.48
338_F	348_R	0.546	0.48
433_G	442_R	0.544	0.48
379_D	387_I	0.542	0.48
42_I	51_F	0.542	0.48
39_P	101_A	0.541	0.47
100_W	254_I	0.537	0.47
187_G	194_F	0.537	0.47
317_G	320_S	0.536	0.47
398_K	408_N	0.536	0.47
371_T	378_K	0.535	0.47
52_G	316_P	0.534	0.47
375_V	380_I	0.534	0.47
439_V	444_W	0.534	0.47
238_Y	359_W	0.533	0.46
29_P	34_V	0.532	0.46
377_Q	405_L	0.530	0.46
103_D	185_V	0.529	0.46
38_D	100_W	0.529	0.46
34_V	140_Y	0.529	0.46
198_M	206_L	0.529	0.46
434_K	444_W	0.529	0.46
398_K	401_I	0.527	0.46
148_K	194_F	0.524	0.45
37_H	52_G	0.524	0.45
215_L	220_H	0.523	0.45
440_F	444_W	0.523	0.45
396_D	416_T	0.522	0.45
271_G	410_T	0.522	0.45
196_L	212_G	0.522	0.45
412_S	433_G	0.521	0.45
392_D	395_A	0.521	0.45
407_K	412_S	0.521	0.45
58_A	68_Q	0.521	0.45
412_S	435_K	0.520	0.45
245_G	317_G	0.519	0.45
99_L	226_P	0.519	0.45
426_T	441_L	0.518	0.45
53_S	246_L	0.518	0.45
54_H	71_T	0.518	0.45
66_W	177_D	0.517	0.44
245_G	252_Y	0.514	0.44
440_F	448_R	0.511	0.44
130_V	144_G	0.510	0.44
226_P	240_Y	0.510	0.44
191_G	222_R	0.510	0.44
239_L	257_A	0.509	0.43
63_L	349_V	0.508	0.43
380_I	386_L	0.508	0.43
35_S	335_H	0.508	0.43
373_K	376_K	0.507	0.43
102_A	117_N	0.507	0.43
115_Y	335_H	0.506	0.43
36_V	50_V	0.506	0.43
56_A	68_Q	0.506	0.43
429_I	436_Y	0.505	0.43
376_K	426_T	0.505	0.43
242_S	319_N	0.504	0.43
365_Y	368_A	0.504	0.43
400_T	411_I	0.503	0.43
109_D	234_T	0.502	0.43
116_Y	131_A	0.502	0.43
375_V	390_G	0.502	0.43
18_F	23_S	0.501	0.42
379_D	393_I	0.500	0.42
396_D	400_T	0.500	0.42

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2x8sA	1	0.936	100	0.271	Contact Map
4kc7A	1	0.9211	100	0.3	Contact Map
4kcaA	2	0.9744	100	0.33	Contact Map
1yrzA	1	0.8081	100	0.427	Contact Map
3c2uA	3	0.8081	100	0.428	Contact Map
1yifA	3	0.806	100	0.43	Contact Map
2exhA	3	0.806	100	0.432	Contact Map
3zxkA	1	0.7825	100	0.46	Contact Map
1gyhA	1	0.6354	100	0.492	Contact Map
4kcbA	1	0.6205	100	0.493	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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