GUDD - BACSU - GREMLIN Contacts

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GUDD - Probable glucarate dehydratase

UniProt:	P42238	Sequences: MSSPIQEQVQKEKRSNIPSISEMKVIPVAGHDSMLLNLSGAHSPFFTRNIVILTDSSGNQGVGEVPGGEHIRRTLELSEPLVVGKSIGAYQAILQTVRKQFGDQDRGGRGNQTFDLRTTVHAVTALEAALLDLLGKFLQEPVAALLGEGKQRDEVKMLGYLFYIGDRNRTTLPYQSDEQSDCAWFRLRHEEALTPEAIVRLAESAQERYGFQDFKLKGGVLRGEEEIEAVTALSKRFPEARITLDPNGAWSLEEAIALCKGKQDVLAYAEDPCGDENGYSAREVMAEFRRATGLPTATNMIATDWREMGHAIQLHAVDIPLADPHFWTMQGSVRVAQMCHDWGLTWGSHSNNHFDISLAMFTHVAAAAPGRITAIDTHWIWQDGQRLTKQPFEISSGCVKVPDKPGLGVDIDMEQVEKAHEIYRKMNLGARNDAIPMQFLISNWEFDRKRPCLVR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	455 (359)
Sequences:	5639 (3436)
Seq/√Len:	181.3

ECOLI - GUDD GUDX RHMD RSPA DGOD AEEP MENC
BACSU - GUDD YITF AEEP MENC
HALSA - Q9HS20 Q9HP25 Q9HQN7
SULSO - GAD Q97VG1 Q97YL9 ARAD Q97YR3 Q97UK8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
154_E	398_C	3.999	1.00
289_R	318_D	3.810	1.00
59_N	133_L	3.509	1.00
85_K	96_T	3.401	1.00
52_I	413_M	2.865	1.00
203_E	236_R	2.857	1.00
24_K	52_I	2.802	1.00
135_G	142_V	2.745	1.00
251_S	254_E	2.716	1.00
312_I	344_L	2.636	1.00
22_E	54_T	2.606	1.00
286_A	290_R	2.598	1.00
55_D	133_L	2.454	1.00
24_K	54_T	2.452	1.00
62_V	411_I	2.442	1.00
259_C	269_A	2.380	1.00
259_C	292_T	2.353	1.00
54_T	60_Q	2.347	1.00
263_Q	293_G	2.324	1.00
127_E	330_Q	2.300	1.00
256_I	291_A	2.296	1.00
59_N	137_F	2.243	1.00
203_E	207_E	2.216	1.00
136_K	410_D	2.202	1.00
277_N	284_V	2.200	1.00
55_D	59_N	2.198	1.00
327_W	335_V	2.172	1.00
287_E	290_R	2.171	1.00
260_K	263_Q	2.153	1.00
19_S	56_S	2.133	1.00
23_M	53_L	2.115	1.00
305_W	331_G	2.095	1.00
283_E	314_L	2.085	1.00
52_I	60_Q	2.085	1.00
156_K	212_Q	2.037	1.00
199_V	236_R	2.026	1.00
389_K	410_D	2.002	1.00
195_P	199_V	1.965	1.00
273_C	288_F	1.962	1.00
287_E	291_A	1.913	1.00
231_T	235_K	1.909	1.00
231_T	265_V	1.907	1.00
309_G	313_Q	1.896	1.00
150_K	153_D	1.877	1.00
286_A	314_L	1.842	1.00
206_Q	240_A	1.831	1.00
252_L	288_F	1.811	1.00
263_Q	294_L	1.791	1.00
21_S	56_S	1.790	1.00
26_I	52_I	1.788	1.00
89_A	92_A	1.780	1.00
194_T	197_A	1.773	1.00
26_I	420_H	1.768	1.00
227_I	261_G	1.745	1.00
258_L	262_K	1.710	1.00
389_K	412_D	1.701	1.00
313_Q	342_W	1.693	1.00
231_T	264_D	1.691	1.00
29_A	45_F	1.688	1.00
140_E	144_A	1.688	1.00
154_E	400_K	1.675	1.00
21_S	54_T	1.655	1.00
200_R	203_E	1.653	1.00
237_F	242_I	1.648	1.00
23_M	79_E	1.640	1.00
334_R	340_H	1.636	1.00
214_F	240_A	1.619	1.00
53_L	130_L	1.616	1.00
55_D	137_F	1.610	1.00
261_G	264_D	1.609	1.00
395_S	400_K	1.603	1.00
59_N	136_K	1.597	1.00
413_M	417_E	1.573	1.00
142_V	407_L	1.559	1.00
50_I	416_V	1.549	1.00
65_V	125_A	1.527	1.00
197_A	200_R	1.519	1.00
312_I	338_M	1.515	1.00
234_S	238_P	1.506	1.00
252_L	287_E	1.501	1.00
202_A	237_F	1.497	1.00
232_A	235_K	1.488	1.00
85_K	92_A	1.483	1.00
93_I	97_V	1.467	1.00
308_M	335_V	1.466	1.00
273_C	284_V	1.452	1.00
50_I	356_I	1.449	1.00
135_G	145_L	1.444	1.00
337_Q	340_H	1.443	1.00
86_S	89_A	1.439	1.00
217_K	247_N	1.430	1.00
310_H	314_L	1.430	1.00
56_S	87_I	1.429	1.00
91_Q	334_R	1.415	1.00
311_A	317_V	1.413	1.00
51_V	125_A	1.412	1.00
390_Q	404_K	1.403	1.00
134_L	138_L	1.396	1.00
332_S	364_V	1.389	1.00
57_S	137_F	1.389	1.00
73_R	76_E	1.388	1.00
91_Q	333_V	1.361	1.00
222_R	225_E	1.359	1.00
142_V	329_M	1.352	1.00
331_G	334_R	1.352	1.00
154_E	396_S	1.348	1.00
256_I	288_F	1.348	1.00
22_E	79_E	1.346	1.00
366_A	401_V	1.336	1.00
78_S	97_V	1.330	1.00
228_E	231_T	1.326	1.00
18_P	56_S	1.322	1.00
303_T	307_E	1.317	1.00
241_R	267_A	1.311	1.00
85_K	93_I	1.306	1.00
206_Q	214_F	1.305	1.00
23_M	82_V	1.302	1.00
323_D	349_H	1.292	1.00
93_I	96_T	1.291	1.00
333_V	337_Q	1.281	1.00
244_L	266_L	1.274	1.00
37_N	42_H	1.271	1.00
415_Q	418_K	1.266	1.00
310_H	313_Q	1.256	1.00
156_K	398_C	1.251	1.00
308_M	320_P	1.250	1.00
257_A	261_G	1.246	1.00
297_A	321_L	1.243	1.00
88_G	137_F	1.241	1.00
203_E	206_Q	1.239	1.00
127_E	131_L	1.229	1.00
312_I	342_W	1.221	1.00
88_G	134_L	1.210	1.00
243_T	267_A	1.203	1.00
234_S	239_E	1.201	1.00
415_Q	419_A	1.200	1.00
196_E	200_R	1.200	1.00
85_K	89_A	1.198	1.00
225_E	228_E	1.196	1.00
298_T	311_A	1.192	1.00
411_I	416_V	1.186	1.00
202_A	214_F	1.184	1.00
263_Q	292_T	1.181	1.00
78_S	126_L	1.177	1.00
227_I	265_V	1.170	1.00
230_V	244_L	1.161	1.00
18_P	57_S	1.158	1.00
97_V	123_V	1.151	1.00
216_L	233_L	1.142	1.00
412_D	415_Q	1.138	1.00
228_E	232_A	1.126	1.00
53_L	82_V	1.123	1.00
52_I	62_V	1.117	1.00
234_S	242_I	1.117	1.00
88_G	138_L	1.108	1.00
340_H	369_P	1.108	1.00
141_P	144_A	1.107	1.00
283_E	310_H	1.107	1.00
143_A	401_V	1.104	1.00
260_K	293_G	1.103	1.00
260_K	291_A	1.103	1.00
92_A	96_T	1.102	1.00
199_V	235_K	1.102	1.00
271_D	321_L	1.101	1.00
230_V	265_V	1.098	1.00
202_A	233_L	1.096	1.00
360_M	407_L	1.091	1.00
195_P	232_A	1.077	1.00
285_M	298_T	1.075	1.00
131_L	330_Q	1.070	1.00
140_E	145_L	1.067	1.00
25_V	72_R	1.066	1.00
204_S	209_Y	1.065	1.00
266_L	294_L	1.053	1.00
322_A	332_S	1.053	1.00
213_D	243_T	1.049	1.00
224_E	261_G	1.044	1.00
90_Y	134_L	1.040	1.00
233_L	237_F	1.034	1.00
414_E	417_E	1.033	1.00
327_W	332_S	1.025	1.00
317_V	320_P	1.022	0.99
138_L	145_L	1.021	0.99
25_V	75_L	1.019	0.99
282_R	311_A	1.014	0.99
227_I	231_T	1.013	0.99
79_E	83_V	1.007	0.99
252_L	284_V	1.004	0.99
204_S	207_E	1.002	0.99
336_A	368_A	0.994	0.99
250_W	258_L	0.989	0.99
150_K	403_D	0.988	0.99
338_M	342_W	0.981	0.99
144_A	403_D	0.979	0.99
54_T	57_S	0.978	0.99
199_V	237_F	0.972	0.99
288_F	296_T	0.968	0.99
128_A	360_M	0.968	0.99
334_R	337_Q	0.967	0.99
218_G	246_P	0.965	0.99
232_A	236_R	0.962	0.99
134_L	145_L	0.959	0.99
230_V	262_K	0.956	0.99
312_I	320_P	0.951	0.99
237_F	240_A	0.944	0.99
135_G	138_L	0.944	0.99
70_H	73_R	0.943	0.99
94_L	97_V	0.933	0.99
246_P	269_A	0.931	0.99
302_A	307_E	0.930	0.99
139_Q	405_P	0.930	0.99
227_I	264_D	0.926	0.99
282_R	316_A	0.926	0.99
216_L	230_V	0.924	0.99
292_T	296_T	0.924	0.99
93_I	130_L	0.923	0.99
223_G	258_L	0.922	0.99
143_A	150_K	0.911	0.99
199_V	233_L	0.909	0.99
130_L	134_L	0.907	0.99
205_A	209_Y	0.907	0.99
297_A	319_I	0.904	0.99
26_I	417_E	0.903	0.99
305_W	338_M	0.901	0.99
304_D	307_E	0.892	0.99
214_F	237_F	0.891	0.99
333_V	367_A	0.889	0.99
324_P	364_V	0.889	0.99
72_R	75_L	0.887	0.99
385_Q	415_Q	0.887	0.99
243_T	268_Y	0.886	0.99
366_A	402_P	0.884	0.99
20_I	53_L	0.884	0.99
309_G	342_W	0.883	0.99
299_N	323_D	0.881	0.98
324_P	332_S	0.878	0.98
82_V	93_I	0.877	0.98
320_P	339_C	0.876	0.98
256_I	287_E	0.876	0.98
362_T	401_V	0.876	0.98
224_E	257_A	0.875	0.98
359_A	409_V	0.874	0.98
289_R	315_H	0.867	0.98
244_L	262_K	0.866	0.98
206_Q	237_F	0.866	0.98
315_H	344_L	0.864	0.98
417_E	420_H	0.862	0.98
133_L	137_F	0.861	0.98
136_K	404_K	0.858	0.98
256_I	260_K	0.857	0.98
50_I	62_V	0.853	0.98
75_L	79_E	0.853	0.98
199_V	203_E	0.850	0.98
154_E	395_S	0.849	0.98
219_G	226_E	0.849	0.98
200_R	204_S	0.846	0.98
199_V	202_A	0.845	0.98
18_P	137_F	0.839	0.98
19_S	86_S	0.838	0.98
195_P	228_E	0.838	0.98
413_M	416_V	0.833	0.98
202_A	236_R	0.828	0.98
54_T	62_V	0.828	0.98
200_R	236_R	0.827	0.98
203_E	237_F	0.827	0.98
49_N	72_R	0.826	0.98
199_V	232_A	0.824	0.98
246_P	250_W	0.818	0.98
198_I	229_A	0.817	0.98
212_Q	396_S	0.816	0.98
252_L	273_C	0.813	0.97
89_A	144_A	0.809	0.97
395_S	398_C	0.808	0.97
394_I	399_V	0.799	0.97
324_P	361_F	0.797	0.97
250_W	269_A	0.796	0.97
340_H	370_G	0.794	0.97
75_L	126_L	0.791	0.97
290_R	315_H	0.779	0.97
321_L	349_H	0.779	0.97
307_E	310_H	0.778	0.97
94_L	98_R	0.777	0.97
302_A	308_M	0.777	0.97
285_M	296_T	0.772	0.97
27_P	47_T	0.770	0.96
94_L	127_E	0.768	0.96
144_A	150_K	0.766	0.96
393_E	400_K	0.763	0.96
302_A	311_A	0.762	0.96
271_D	299_N	0.762	0.96
153_D	403_D	0.759	0.96
224_E	228_E	0.758	0.96
253_E	257_A	0.758	0.96
377_T	382_Q	0.757	0.96
159_G	211_F	0.757	0.96
143_A	403_D	0.757	0.96
390_Q	410_D	0.756	0.96
132_D	136_K	0.754	0.96
392_F	399_V	0.753	0.96
322_A	346_W	0.753	0.96
87_I	130_L	0.751	0.96
202_A	232_A	0.749	0.96
226_E	250_W	0.748	0.96
256_I	292_T	0.746	0.96
282_R	310_H	0.742	0.96
206_Q	236_R	0.739	0.96
82_V	130_L	0.738	0.95
27_P	72_R	0.738	0.95
400_K	403_D	0.736	0.95
329_M	364_V	0.736	0.95
159_G	205_A	0.736	0.95
252_L	256_I	0.735	0.95
81_L	96_T	0.733	0.95
287_E	315_H	0.731	0.95
213_D	241_R	0.727	0.95
21_S	57_S	0.727	0.95
259_C	294_L	0.726	0.95
70_H	75_L	0.724	0.95
95_Q	151_Q	0.722	0.95
218_G	262_K	0.722	0.95
362_T	402_P	0.721	0.95
74_T	122_A	0.720	0.95
63_G	125_A	0.719	0.95
329_M	333_V	0.718	0.95
86_S	144_A	0.716	0.95
289_R	296_T	0.714	0.95
227_I	262_K	0.712	0.95
201_L	204_S	0.711	0.94
75_L	80_P	0.710	0.94
157_M	399_V	0.709	0.94
135_G	140_E	0.704	0.94
62_V	413_M	0.701	0.94
267_A	295_P	0.701	0.94
90_Y	97_V	0.694	0.94
21_S	55_D	0.693	0.94
49_N	65_V	0.693	0.94
277_N	343_G	0.693	0.94
273_C	285_M	0.693	0.94
135_G	405_P	0.692	0.94
359_A	362_T	0.691	0.94
309_G	338_M	0.690	0.94
379_W	382_Q	0.687	0.93
82_V	126_L	0.687	0.93
76_E	79_E	0.684	0.93
133_L	136_K	0.684	0.93
23_M	126_L	0.684	0.93
79_E	126_L	0.684	0.93
362_T	399_V	0.681	0.93
134_L	137_F	0.679	0.93
355_D	388_T	0.679	0.93
257_A	260_K	0.679	0.93
404_K	410_D	0.676	0.93
87_I	133_L	0.675	0.93
321_L	348_S	0.674	0.93
94_L	330_Q	0.674	0.93
143_A	363_H	0.670	0.93
356_I	409_V	0.669	0.93
286_A	315_H	0.669	0.93
299_N	321_L	0.668	0.92
18_P	87_I	0.667	0.92
146_L	329_M	0.662	0.92
70_H	77_L	0.661	0.92
218_G	244_L	0.660	0.92
98_R	331_G	0.660	0.92
60_Q	410_D	0.657	0.92
102_G	118_T	0.656	0.92
308_M	339_C	0.652	0.92
71_I	74_T	0.651	0.91
272_P	296_T	0.650	0.91
283_E	313_Q	0.650	0.91
141_P	363_H	0.647	0.91
93_I	126_L	0.646	0.91
131_L	142_V	0.646	0.91
233_L	236_R	0.646	0.91
212_Q	239_E	0.645	0.91
29_A	47_T	0.644	0.91
308_M	322_A	0.642	0.91
60_Q	136_K	0.642	0.91
218_G	250_W	0.640	0.91
76_E	80_P	0.638	0.91
266_L	269_A	0.635	0.90
72_R	76_E	0.633	0.90
97_V	126_L	0.632	0.90
71_I	75_L	0.632	0.90
271_D	301_I	0.631	0.90
378_H	381_W	0.631	0.90
150_K	401_V	0.631	0.90
329_M	360_M	0.630	0.90
62_V	136_K	0.630	0.90
70_H	74_T	0.628	0.90
414_E	418_K	0.628	0.90
359_A	407_L	0.627	0.90
128_A	356_I	0.626	0.90
346_W	368_A	0.625	0.90
66_P	325_H	0.623	0.90
269_A	294_L	0.623	0.90
77_L	80_P	0.622	0.90
299_N	348_S	0.621	0.89
376_D	380_I	0.615	0.89
225_E	229_A	0.614	0.89
356_I	411_I	0.609	0.89
336_A	370_G	0.609	0.89
101_F	123_V	0.608	0.88
73_R	77_L	0.606	0.88
255_A	273_C	0.606	0.88
141_P	404_K	0.605	0.88
56_S	59_N	0.604	0.88
196_E	199_V	0.604	0.88
285_M	288_F	0.603	0.88
288_F	291_A	0.598	0.88
141_P	145_L	0.597	0.88
78_S	101_F	0.597	0.88
161_L	164_I	0.596	0.87
285_M	316_A	0.595	0.87
22_E	56_S	0.595	0.87
18_P	86_S	0.595	0.87
298_T	317_V	0.594	0.87
90_Y	130_L	0.594	0.87
152_R	155_V	0.594	0.87
90_Y	93_I	0.593	0.87
299_N	349_H	0.592	0.87
138_L	141_P	0.590	0.87
31_H	419_A	0.590	0.87
378_H	382_Q	0.589	0.87
65_V	121_H	0.589	0.87
57_S	87_I	0.588	0.87
38_L	43_S	0.587	0.87
276_E	306_R	0.587	0.87
223_G	227_I	0.586	0.87
61_G	129_A	0.585	0.87
234_S	265_V	0.585	0.87
263_Q	266_L	0.583	0.86
321_L	347_G	0.582	0.86
271_D	300_M	0.581	0.86
196_E	228_E	0.581	0.86
97_V	101_F	0.580	0.86
248_G	275_D	0.579	0.86
254_E	257_A	0.578	0.86
161_L	205_A	0.577	0.86
381_W	384_G	0.577	0.86
87_I	137_F	0.576	0.86
282_R	314_L	0.576	0.86
105_D	117_R	0.575	0.86
268_Y	299_N	0.572	0.85
230_V	266_L	0.571	0.85
212_Q	398_C	0.570	0.85
268_Y	347_G	0.567	0.85
26_I	62_V	0.567	0.85
146_L	333_V	0.565	0.85
95_Q	99_K	0.565	0.85
311_A	316_A	0.565	0.85
345_T	372_I	0.562	0.84
233_L	242_I	0.562	0.84
380_I	383_D	0.561	0.84
96_T	99_K	0.560	0.84
195_P	225_E	0.560	0.84
227_I	230_V	0.559	0.84
162_F	271_D	0.557	0.84
78_S	123_V	0.556	0.84
138_L	142_V	0.556	0.84
273_C	298_T	0.556	0.84
295_P	319_I	0.556	0.84
328_T	331_G	0.555	0.84
130_L	133_L	0.553	0.83
142_V	333_V	0.552	0.83
128_A	407_L	0.552	0.83
38_L	41_A	0.552	0.83
35_L	43_S	0.546	0.83
338_M	341_D	0.546	0.83
35_L	42_H	0.542	0.82
74_T	77_L	0.541	0.82
32_D	46_F	0.541	0.82
51_V	126_L	0.539	0.82
160_Y	163_Y	0.538	0.82
206_Q	239_E	0.538	0.82
286_A	316_A	0.536	0.81
28_V	419_A	0.535	0.81
195_P	202_A	0.535	0.81
226_E	247_N	0.533	0.81
367_A	370_G	0.532	0.81
56_S	137_F	0.532	0.81
158_L	375_I	0.531	0.81
89_A	153_D	0.529	0.81
385_Q	418_K	0.529	0.81
299_N	322_A	0.529	0.81
196_E	232_A	0.529	0.81
325_H	351_N	0.528	0.80
385_Q	419_A	0.528	0.80
333_V	368_A	0.527	0.80
332_S	346_W	0.526	0.80
103_D	106_R	0.525	0.80
34_M	201_L	0.524	0.80
23_M	51_V	0.524	0.80
28_V	411_I	0.523	0.80
348_S	365_A	0.523	0.80
306_R	310_H	0.523	0.80
49_N	71_I	0.521	0.80
32_D	36_L	0.521	0.80
296_T	316_A	0.521	0.80
196_E	417_E	0.519	0.79
66_P	349_H	0.519	0.79
216_L	244_L	0.519	0.79
39_S	352_N	0.519	0.79
359_A	363_H	0.519	0.79
350_S	358_L	0.518	0.79
158_L	321_L	0.518	0.79
218_G	226_E	0.517	0.79
325_H	357_S	0.517	0.79
18_P	21_S	0.517	0.79
135_G	407_L	0.517	0.79
98_R	123_V	0.516	0.79
19_S	83_V	0.515	0.79
242_I	266_L	0.515	0.79
20_I	23_M	0.514	0.79
255_A	269_A	0.514	0.79
378_H	383_D	0.513	0.79
67_G	121_H	0.512	0.78
320_P	335_V	0.512	0.78
142_V	363_H	0.512	0.78
50_I	411_I	0.512	0.78
276_E	282_R	0.511	0.78
268_Y	380_I	0.510	0.78
217_K	226_E	0.510	0.78
244_L	259_C	0.510	0.78
30_G	354_F	0.509	0.78
234_S	240_A	0.508	0.78
87_I	90_Y	0.508	0.78
227_I	258_L	0.507	0.78
131_L	135_G	0.507	0.78
40_G	46_F	0.506	0.78
376_D	379_W	0.505	0.77
329_M	332_S	0.503	0.77
39_S	249_A	0.503	0.77
80_P	126_L	0.502	0.77
378_H	384_G	0.502	0.77
320_P	344_L	0.501	0.77
81_L	85_K	0.500	0.77
128_A	142_V	0.500	0.77

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3mznA	2	0.9648	100	0.274	Contact Map
3pfrA	2	0.9209	100	0.287	Contact Map
3p0wA	2	0.9253	100	0.287	Contact Map
4hyrA	4	0.9538	100	0.292	Contact Map
4g8tA	3	0.9516	100	0.302	Contact Map
3vdgA	2	0.9143	100	0.323	Contact Map
3vc5A	2	0.8989	100	0.323	Contact Map
4it1A	3	0.9187	100	0.327	Contact Map
3va8A	2	0.9121	100	0.329	Contact Map
3s5sA	2	0.778	100	0.345	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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