GS30 - BACSU - GREMLIN Contacts

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GS30 - General stress protein 30

UniProt:	P42101	Sequences: MTVQEIKGKKLVKGIAPNVEPEALLNDKRKVFLFGSPSYTNIGDQAIAYAEEKFIKNHFPYYEYIEIMDYATDEGIELVKEIIREDDIVCFTGGGNLGNLYLDIEEDRRKVFSAFKDYKSISLPQSVYFEDTEEGQKEKKKTQDAYHQNTNLTIAARETQTLDVVKETFNSNVIFTPDMVLSLDIVPRELERDGVLFILRADKEKVTDEDFISQMKQWAEKTTYTERTDTVLDTVDTIDYADREKHFMEMLDRIGSSKLVITDRLHAMIFSIITKTPCLVFGNSYGKAKHSYRDWLESLNFIEYTDKNDVEELERMIDRLLQAEPNDVDLSKDFQPLIDFFAS	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	343 (291)
Sequences:	400 (354)
Seq/√Len:	20.8

BACSU - GS30 EPSI EPSO

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
39_Y	92_T	7.724	1.00
50_A	180_V	4.487	1.00
96_N	108_R	4.338	1.00
33_L	51_E	4.076	1.00
158_E	291_S	4.024	1.00
268_M	296_L	4.007	1.00
268_M	278_C	3.586	1.00
194_G	258_K	3.583	1.00
105_E	109_R	3.172	1.00
282_G	289_K	3.094	0.99
156_A	165_V	3.073	0.99
47_I	93_G	3.008	0.99
47_I	265_L	2.997	0.99
127_V	165_V	2.936	0.99
51_E	92_T	2.936	0.99
123_L	179_M	2.738	0.99
177_P	295_W	2.706	0.99
153_T	174_I	2.582	0.98
155_A	176_T	2.526	0.98
147_H	171_S	2.470	0.97
91_F	111_V	2.423	0.97
93_G	265_L	2.343	0.96
120_S	149_N	2.310	0.96
37_P	98_G	2.303	0.96
127_V	161_T	2.267	0.96
196_L	257_S	2.212	0.95
278_C	296_L	2.202	0.95
54_F	179_M	2.095	0.93
47_I	95_G	1.899	0.89
264_R	287_K	1.788	0.86
158_E	285_Y	1.757	0.85
41_N	44_D	1.716	0.84
159_T	163_D	1.698	0.83
41_N	287_K	1.685	0.83
313_L	317_I	1.681	0.82
105_E	108_R	1.680	0.82
33_L	123_L	1.678	0.82
35_G	92_T	1.655	0.81
163_D	167_E	1.633	0.81
113_S	148_Q	1.595	0.79
109_R	138_E	1.580	0.78
154_I	165_V	1.579	0.78
99_N	105_E	1.573	0.78
210_D	261_I	1.568	0.78
40_T	158_E	1.532	0.76
79_V	89_V	1.491	0.74
96_N	101_Y	1.476	0.73
46_A	180_V	1.475	0.73
31_V	48_A	1.465	0.73
37_P	51_E	1.455	0.72
41_N	200_R	1.452	0.72
41_N	264_R	1.424	0.70
116_K	149_N	1.411	0.70
181_L	272_I	1.403	0.69
99_N	130_E	1.379	0.68
97_L	105_E	1.367	0.67
95_G	101_Y	1.367	0.67
51_E	179_M	1.365	0.67
88_I	118_Y	1.356	0.67
303_E	319_R	1.352	0.66
38_S	123_L	1.352	0.66
98_G	108_R	1.345	0.66
124_P	204_E	1.338	0.66
50_A	183_L	1.318	0.64
280_V	288_A	1.313	0.64
95_G	126_S	1.309	0.64
155_A	174_I	1.309	0.64
92_T	96_N	1.308	0.64
127_V	154_I	1.307	0.64
156_A	169_F	1.292	0.63
48_A	250_M	1.291	0.63
250_M	253_R	1.285	0.62
113_S	256_S	1.284	0.62
125_Q	177_P	1.281	0.62
105_E	146_Y	1.272	0.62
247_F	251_L	1.265	0.61
278_C	302_I	1.256	0.61
198_I	254_I	1.256	0.61
36_S	66_E	1.244	0.60
54_F	93_G	1.243	0.60
55_I	90_C	1.238	0.59
35_G	282_G	1.227	0.59
34_F	91_F	1.226	0.59
95_G	98_G	1.221	0.58
98_G	129_F	1.202	0.57
117_D	144_D	1.194	0.57
280_V	292_Y	1.193	0.56
54_F	283_N	1.184	0.56
217_Q	221_K	1.170	0.55
31_V	59_F	1.170	0.55
181_L	291_S	1.167	0.55
108_R	291_S	1.165	0.55
259_L	279_L	1.162	0.54
37_P	48_A	1.139	0.53
148_Q	271_S	1.139	0.53
37_P	45_Q	1.127	0.52
188_R	255_G	1.125	0.52
47_I	101_Y	1.120	0.52
200_R	287_K	1.119	0.52
94_G	287_K	1.112	0.51
37_P	124_P	1.110	0.51
158_E	272_I	1.098	0.50
282_G	286_G	1.094	0.50
48_A	126_S	1.090	0.50
157_R	266_H	1.076	0.49
31_V	90_C	1.069	0.48
36_S	45_Q	1.066	0.48
30_K	87_D	1.057	0.48
205_K	219_A	1.057	0.48
129_F	274_T	1.055	0.47
198_I	263_D	1.054	0.47
119_K	151_N	1.054	0.47
239_D	243_R	1.040	0.46
309_D	314_E	1.039	0.46
51_E	123_L	1.037	0.46
175_F	294_D	1.028	0.46
39_Y	140_K	1.028	0.46
41_N	94_G	1.026	0.45
99_N	109_R	1.026	0.45
44_D	94_G	1.026	0.45
189_E	260_V	1.017	0.45
305_T	313_L	1.017	0.45
276_T	301_F	1.016	0.45
47_I	54_F	1.014	0.45
46_A	280_V	1.012	0.45
163_D	166_K	1.011	0.45
177_P	267_A	1.009	0.44
305_T	312_E	1.001	0.44
29_R	87_D	0.998	0.44
126_S	198_I	0.998	0.44
300_N	311_E	0.997	0.44
123_L	165_V	0.997	0.44
280_V	285_Y	0.997	0.44
101_Y	291_S	0.987	0.43
50_A	182_S	0.985	0.43
260_V	271_S	0.983	0.43
202_D	262_T	0.982	0.43
90_C	123_L	0.982	0.43
81_E	220_E	0.978	0.42
33_L	90_C	0.973	0.42
98_G	105_E	0.971	0.42
212_I	216_K	0.966	0.42
45_Q	101_Y	0.964	0.41
311_E	316_M	0.960	0.41
123_L	281_F	0.960	0.41
138_E	146_Y	0.957	0.41
309_D	320_L	0.953	0.41
44_D	287_K	0.951	0.41
166_K	281_F	0.949	0.41
175_F	295_W	0.944	0.40
170_N	173_V	0.944	0.40
174_I	193_D	0.943	0.40
56_K	159_T	0.940	0.40
54_F	95_G	0.938	0.40
140_K	144_D	0.938	0.40
285_Y	291_S	0.936	0.40
62_Y	243_R	0.929	0.39
95_G	283_N	0.927	0.39
164_V	168_T	0.925	0.39
201_A	209_E	0.925	0.39
131_D	189_E	0.925	0.39
288_A	292_Y	0.925	0.39
99_N	132_T	0.922	0.39
101_Y	108_R	0.920	0.39
289_K	304_Y	0.917	0.38
202_D	295_W	0.916	0.38
156_A	265_L	0.913	0.38
195_V	258_K	0.912	0.38
79_V	279_L	0.910	0.38
290_H	294_D	0.905	0.38
48_A	105_E	0.899	0.37
70_Y	302_I	0.899	0.37
55_I	112_F	0.898	0.37
44_D	95_G	0.895	0.37
314_E	317_I	0.890	0.37
139_K	143_Q	0.888	0.37
271_S	300_N	0.885	0.37
166_K	248_M	0.884	0.36
98_G	101_Y	0.884	0.36
31_V	174_I	0.879	0.36
238_I	242_D	0.878	0.36
196_L	285_Y	0.875	0.36
47_I	50_A	0.875	0.36
139_K	164_V	0.873	0.36
194_G	257_S	0.867	0.35
212_I	219_A	0.865	0.35
109_R	142_T	0.863	0.35
268_M	276_T	0.863	0.35
35_G	96_N	0.860	0.35
243_R	268_M	0.857	0.35
43_G	157_R	0.856	0.35
43_G	266_H	0.856	0.35
195_V	300_N	0.856	0.35
42_I	247_F	0.854	0.35
260_V	275_K	0.854	0.35
50_A	176_T	0.852	0.35
117_D	173_V	0.851	0.34
99_N	149_N	0.849	0.34
37_P	179_M	0.846	0.34
50_A	274_T	0.842	0.34
31_V	51_E	0.840	0.34
36_S	142_T	0.839	0.34
62_Y	65_I	0.833	0.33
179_M	286_G	0.833	0.33
94_G	264_R	0.830	0.33
245_K	275_K	0.830	0.33
100_L	284_S	0.826	0.33
45_Q	246_H	0.823	0.33
61_Y	79_V	0.820	0.33
33_L	254_I	0.816	0.32
261_I	302_I	0.816	0.32
97_L	230_T	0.814	0.32
129_F	169_F	0.813	0.32
30_K	63_E	0.812	0.32
40_T	269_I	0.806	0.32
99_N	269_I	0.806	0.32
268_M	291_S	0.804	0.32
24_L	31_V	0.803	0.32
102_L	109_R	0.801	0.32
126_S	233_D	0.797	0.31
101_Y	267_A	0.796	0.31
95_G	204_E	0.793	0.31
265_L	288_A	0.789	0.31
200_R	264_R	0.788	0.31
142_T	146_Y	0.787	0.31
50_A	95_G	0.784	0.31
33_L	64_Y	0.779	0.30
111_V	160_Q	0.778	0.30
93_G	283_N	0.774	0.30
35_G	108_R	0.771	0.30
108_R	146_Y	0.771	0.30
78_L	269_I	0.770	0.30
117_D	166_K	0.768	0.30
180_V	296_L	0.768	0.30
91_F	108_R	0.764	0.29
124_P	202_D	0.763	0.29
313_L	318_D	0.761	0.29
163_D	225_T	0.761	0.29
47_I	177_P	0.760	0.29
262_T	280_V	0.757	0.29
226_E	229_D	0.756	0.29
47_I	202_D	0.756	0.29
51_E	285_Y	0.754	0.29
28_K	280_V	0.753	0.29
34_F	89_V	0.750	0.29
154_I	167_E	0.750	0.29
197_F	288_A	0.748	0.29
267_A	295_W	0.747	0.29
119_K	145_A	0.747	0.29
205_K	288_A	0.746	0.28
162_L	292_Y	0.746	0.28
129_F	142_T	0.737	0.28
38_S	283_N	0.737	0.28
84_R	87_D	0.736	0.28
47_I	179_M	0.734	0.28
46_A	276_T	0.733	0.28
124_P	262_T	0.732	0.28
237_T	271_S	0.732	0.28
178_D	265_L	0.730	0.28
131_D	279_L	0.727	0.27
131_D	228_T	0.726	0.27
267_A	301_F	0.722	0.27
203_K	275_K	0.721	0.27
94_G	266_H	0.720	0.27
94_G	157_R	0.720	0.27
296_L	302_I	0.719	0.27
37_P	129_F	0.719	0.27
44_D	200_R	0.718	0.27
264_R	271_S	0.718	0.27
45_Q	278_C	0.716	0.27
53_K	279_L	0.712	0.27
67_I	268_M	0.711	0.27
89_V	115_F	0.710	0.27
266_H	287_K	0.707	0.26
157_R	287_K	0.707	0.26
197_F	297_E	0.706	0.26
96_N	291_S	0.706	0.26
126_S	262_T	0.702	0.26
112_F	142_T	0.700	0.26
113_S	117_D	0.700	0.26
69_D	103_D	0.699	0.26
156_A	161_T	0.699	0.26
37_P	92_T	0.698	0.26
47_I	283_N	0.698	0.26
72_T	75_G	0.697	0.26
205_K	263_D	0.697	0.26
36_S	55_I	0.696	0.26
165_V	268_M	0.696	0.26
106_E	109_R	0.694	0.26
271_S	320_L	0.691	0.26
54_F	58_H	0.689	0.26
243_R	296_L	0.688	0.25
151_N	183_L	0.686	0.25
265_L	291_S	0.683	0.25
180_V	270_F	0.683	0.25
95_G	108_R	0.683	0.25
144_D	218_W	0.682	0.25
195_V	201_A	0.682	0.25
124_P	178_D	0.676	0.25
44_D	47_I	0.675	0.25
32_F	83_I	0.674	0.25
56_K	97_L	0.673	0.25
95_G	200_R	0.671	0.25
85_E	195_V	0.669	0.25
93_G	101_Y	0.668	0.24
72_T	76_I	0.667	0.24
239_D	242_D	0.667	0.24
35_G	50_A	0.664	0.24
98_G	118_Y	0.664	0.24
188_R	252_D	0.664	0.24
128_Y	158_E	0.661	0.24
44_D	264_R	0.660	0.24
127_V	147_H	0.654	0.24
62_Y	145_A	0.653	0.24
199_L	317_I	0.650	0.24
314_E	318_D	0.649	0.24
138_E	142_T	0.648	0.24
64_Y	121_I	0.648	0.24
257_S	281_F	0.647	0.23
83_I	126_S	0.644	0.23
191_E	259_L	0.639	0.23
314_E	319_R	0.638	0.23
157_R	178_D	0.637	0.23
178_D	266_H	0.637	0.23
98_G	124_P	0.636	0.23
50_A	291_S	0.635	0.23
165_V	173_V	0.633	0.23
125_Q	282_G	0.633	0.23
35_G	176_T	0.632	0.23
112_F	166_K	0.631	0.23
47_I	200_R	0.628	0.23
146_Y	288_A	0.627	0.23
204_E	295_W	0.626	0.22
99_N	158_E	0.626	0.22
50_A	93_G	0.623	0.22
127_V	269_I	0.621	0.22
66_E	243_R	0.620	0.22
122_S	154_I	0.619	0.22
88_I	313_L	0.619	0.22
260_V	279_L	0.619	0.22
248_M	269_I	0.618	0.22
48_A	90_C	0.616	0.22
122_S	247_F	0.613	0.22
112_F	149_N	0.612	0.22
126_S	254_I	0.608	0.22
204_E	262_T	0.607	0.22
126_S	290_H	0.607	0.22
282_G	285_Y	0.605	0.22
89_V	123_L	0.602	0.21
200_R	204_E	0.602	0.21
45_Q	254_I	0.602	0.21
97_L	282_G	0.602	0.21
117_D	225_T	0.602	0.21
47_I	204_E	0.601	0.21
57_N	114_A	0.597	0.21
196_L	260_V	0.594	0.21
315_R	320_L	0.594	0.21
67_I	296_L	0.593	0.21
108_R	295_W	0.593	0.21
173_V	281_F	0.592	0.21
255_G	271_S	0.591	0.21
278_C	291_S	0.590	0.21
268_M	274_T	0.588	0.21
271_S	278_C	0.587	0.21
193_D	255_G	0.582	0.21
152_L	298_S	0.582	0.21
225_T	258_K	0.579	0.20
39_Y	177_P	0.577	0.20
46_A	251_L	0.577	0.20
179_M	285_Y	0.576	0.20
167_E	260_V	0.574	0.20
105_E	251_L	0.573	0.20
156_A	229_D	0.571	0.20
42_I	250_M	0.571	0.20
125_Q	270_F	0.567	0.20
90_C	116_K	0.567	0.20
94_G	200_R	0.566	0.20
50_A	90_C	0.564	0.20
49_Y	273_I	0.562	0.20
155_A	254_I	0.560	0.20
30_K	79_V	0.560	0.20
98_G	295_W	0.560	0.20
172_N	222_T	0.558	0.20
119_K	288_A	0.557	0.19
215_M	228_T	0.556	0.19
286_G	289_K	0.555	0.19
45_Q	243_R	0.553	0.19
269_I	291_S	0.553	0.19
123_L	279_L	0.552	0.19
161_T	266_H	0.552	0.19
157_R	161_T	0.552	0.19
101_Y	204_E	0.551	0.19
64_Y	73_D	0.550	0.19
195_V	259_L	0.550	0.19
160_Q	271_S	0.549	0.19
183_L	273_I	0.549	0.19
157_R	264_R	0.545	0.19
53_K	56_K	0.541	0.19
253_R	269_I	0.540	0.19
124_P	263_D	0.537	0.19
95_G	282_G	0.536	0.19
283_N	286_G	0.536	0.19
269_I	288_A	0.535	0.19
272_I	301_F	0.534	0.19
202_D	287_K	0.533	0.18
51_E	283_N	0.533	0.18
226_E	232_L	0.526	0.18
177_P	202_D	0.525	0.18
76_I	156_A	0.524	0.18
31_V	155_A	0.524	0.18
126_S	149_N	0.523	0.18
35_G	283_N	0.523	0.18
68_M	160_Q	0.522	0.18
47_I	178_D	0.521	0.18
92_T	108_R	0.518	0.18
48_A	101_Y	0.517	0.18
161_T	264_R	0.517	0.18
45_Q	48_A	0.517	0.18
95_G	202_D	0.515	0.18
105_E	196_L	0.514	0.18
120_S	145_A	0.514	0.18
205_K	227_R	0.514	0.18
311_E	315_R	0.514	0.18
105_E	295_W	0.514	0.18
41_N	157_R	0.510	0.18
41_N	266_H	0.510	0.18
71_A	278_C	0.508	0.18
229_D	254_I	0.507	0.17
178_D	204_E	0.506	0.17
112_F	118_Y	0.506	0.17
173_V	224_Y	0.505	0.17
59_F	243_R	0.503	0.17
52_E	66_E	0.503	0.17
48_A	55_I	0.502	0.17
177_P	204_E	0.500	0.17
163_D	253_R	0.500	0.17

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2gt1A	1	0.8163	96.3	0.926	Contact Map
3l7iA	4	0.9329	95.5	0.931	Contact Map
3tovA	1	0.8105	94.9	0.933	Contact Map
3rhzA	4	0.8484	91	0.942	Contact Map
4nesA	2	0.8251	81.5	0.95	Contact Map
3oy2A	2	0.8513	80.3	0.95	Contact Map
2iw1A	1	0.8601	78.8	0.951	Contact Map
1pswA	1	0.8076	73.9	0.953	Contact Map
2x6qA	1	0.9125	73.4	0.953	Contact Map
4w6qA	4	0.8426	72.5	0.953	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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