GREMLIN Database
PKSI - Putative polyketide biosynthesis enoyl-CoA isomerase PksI
UniProt: P40802 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 249 (238)
Sequences: 30241 (19880)
Seq/√Len: 1288.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
126_V164_S3.2881.00
123_R181_P3.1221.00
178_K188_Y3.0891.00
121_L176_P2.9641.00
188_Y191_E2.8751.00
99_A116_A2.8471.00
17_Q190_V2.6561.00
45_R94_E2.5421.00
42_E89_L2.4631.00
163_G195_E2.3321.00
124_E182_R2.3051.00
218_Q221_R2.2531.00
169_D172_K2.2091.00
157_E163_G2.1891.00
166_R169_D2.1831.00
133_K199_K2.0771.00
154_L173_R2.0761.00
26_K61_Y2.0701.00
159_L207_L2.0621.00
17_Q56_T2.0611.00
38_I89_L1.9451.00
32_E36_D1.9191.00
14_A51_K1.9071.00
100_A189_A1.9071.00
106_I128_T1.8751.00
123_R168_A1.8721.00
21_Q58_Y1.8631.00
19_K58_Y1.8481.00
26_K60_N1.8281.00
11_I16_I1.7991.00
100_A186_L1.7991.00
156_Q212_V1.7931.00
120_I180_L1.7911.00
157_E177_F1.7791.00
19_K56_T1.7771.00
117_D208_K1.7581.00
9_I16_I1.7501.00
16_I44_I1.7351.00
43_Y47_N1.7331.00
184_E187_D1.7331.00
190_V194_Q1.7281.00
11_I47_N1.7231.00
53_V93_C1.6851.00
123_R182_R1.6581.00
169_D173_R1.6501.00
152_F156_Q1.5941.00
16_I50_Y1.5931.00
168_A172_K1.5761.00
120_I192_L1.5501.00
119_V149_K1.5191.00
184_E188_Y1.5131.00
157_E178_K1.5071.00
121_L127_Y1.5061.00
44_I53_V1.4951.00
113_G119_V1.4791.00
119_V153_S1.4781.00
122_S182_R1.4741.00
180_L188_Y1.4601.00
52_A193_A1.4431.00
45_R89_L1.4391.00
12_E190_V1.4351.00
18_V40_A1.4341.00
156_Q211_L1.4341.00
60_N102_Q1.4281.00
88_S92_D1.4181.00
101_M113_G1.4101.00
13_S194_Q1.4091.00
88_S220_P1.3961.00
91_L145_I1.3881.00
156_Q160_L1.3831.00
171_E179_V1.3811.00
101_M105_G1.3711.00
102_Q122_S1.3561.00
110_F129_A1.3441.00
102_Q124_E1.3421.00
12_E194_Q1.3381.00
41_F93_C1.3371.00
206_T232_K1.3341.00
56_T102_Q1.3161.00
90_A97_V1.3151.00
38_I42_E1.3141.00
158_I165_Y1.3131.00
12_E17_Q1.3071.00
154_L157_E1.3061.00
185_V189_A1.2941.00
163_G169_D1.2841.00
31_Q70_G1.2831.00
9_I44_I1.2501.00
128_T164_S1.2431.00
30_S33_L1.2341.00
22_D25_H1.2281.00
209_D213_A1.2221.00
132_M162_A1.2151.00
38_I85_N1.2131.00
153_S177_F1.2011.00
104_H126_V1.1921.00
186_L190_V1.1901.00
154_L169_D1.1901.00
36_D40_A1.1891.00
45_R93_C1.1881.00
239_V243_I1.1831.00
61_Y104_H1.1731.00
217_D221_R1.1711.00
186_L189_A1.1691.00
199_K204_L1.1611.00
20_M33_L1.1541.00
17_Q186_L1.1521.00
56_T186_L1.1291.00
25_H69_E1.1241.00
165_Y173_R1.1231.00
20_M63_A1.1151.00
217_D225_Q1.1061.00
63_A109_G1.0961.00
122_S185_V1.0901.00
213_A225_Q1.0771.00
167_G179_V1.0761.00
151_G154_L1.0721.00
36_D39_Q1.0581.00
39_Q43_Y1.0461.00
202_N238_E1.0461.00
31_Q35_D1.0401.00
187_D191_E1.0331.00
53_V95_I1.0301.00
92_D220_P1.0301.00
111_V142_A1.0171.00
118_I192_L1.0021.00
193_A197_A0.9971.00
150_L154_L0.9931.00
97_V116_A0.9891.00
22_D67_T0.9881.00
221_R224_E0.9561.00
40_A43_Y0.9501.00
149_K153_S0.9491.00
143_T226_E0.9441.00
94_E205_V0.9431.00
171_E176_P0.9431.00
59_D102_Q0.9421.00
17_Q54_I0.9331.00
56_T100_A0.9271.00
54_I189_A0.9251.00
201_R205_V0.9251.00
168_A171_E0.9231.00
11_I43_Y0.9161.00
240_K244_K0.9151.00
126_V166_R0.9151.00
181_P184_E0.9101.00
100_A185_V0.9081.00
110_F127_Y0.9071.00
209_D229_M0.9071.00
53_V97_V0.9061.00
11_I49_K0.8971.00
50_Y95_I0.8921.00
190_V193_A0.8881.00
34_T86_L0.8871.00
132_M207_L0.8811.00
48_P95_I0.8791.00
15_I52_A0.8691.00
26_K64_S0.8671.00
206_T209_D0.8621.00
137_T233_T0.8511.00
241_S245_G0.8501.00
102_Q185_V0.8491.00
206_T229_M0.8461.00
10_E19_K0.8451.00
225_Q228_M0.8401.00
191_E195_E0.8361.00
144_F222_V0.8351.00
101_M127_Y0.8291.00
206_T233_T0.8291.00
111_V131_F0.8291.00
152_F215_L0.8261.00
118_I177_F0.8161.00
122_S183_A0.8111.00
41_F90_A0.8101.00
106_I164_S0.8101.00
42_E46_Q0.8091.00
10_E17_Q0.8081.00
159_L211_L0.8061.00
101_M119_V0.8061.00
171_E178_K0.8041.00
157_E161_N0.7991.00
150_L158_I0.7971.00
44_I50_Y0.7941.00
242_R245_G0.7921.00
42_E85_N0.7921.00
210_H229_M0.7891.00
158_I173_R0.7871.00
55_L116_A0.7871.00
38_I86_L0.7841.00
107_G130_N0.7821.00
123_R179_V0.7771.00
188_Y192_L0.7771.00
18_V53_V0.7731.00
30_S70_G0.7731.00
187_D190_V0.7731.00
178_K192_L0.7711.00
45_R95_I0.7681.00
69_E247_Y0.7671.00
133_K164_S0.7661.00
61_Y102_Q0.7661.00
35_D39_Q0.7621.00
189_A193_A0.7551.00
75_Q78_L0.7551.00
238_E242_R0.7541.00
146_V150_L0.7521.00
225_Q229_M0.7491.00
29_F33_L0.7441.00
87_Y115_F0.7411.00
51_K201_R0.7411.00
237_E240_K0.7411.00
94_E209_D0.7381.00
131_F139_G0.7311.00
227_L231_E0.7311.00
191_E194_Q0.7251.00
241_S244_K0.7241.00
22_D30_S0.7241.00
26_K104_H0.7221.00
200_P238_E0.7221.00
93_C97_V0.7131.00
11_I50_Y0.7111.00
167_G176_P0.7081.00
195_E199_K0.7031.00
219_L223_I0.7001.00
131_F230_H0.7001.00
151_G155_A0.7001.00
213_A217_D0.6981.00
170_L175_V0.6951.00
131_F138_P0.6931.00
11_I14_A0.6891.00
114_L175_V0.6891.00
229_M232_K0.6881.00
196_L204_L0.6861.00
224_E228_M0.6861.00
102_Q182_R0.6741.00
180_L185_V0.6701.00
55_L97_V0.6691.00
243_I247_Y0.6681.00
161_N192_L0.6681.00
33_L36_D0.6661.00
120_I178_K0.6631.00
44_I95_I0.6631.00
101_M125_S0.6631.00
57_G60_N0.6611.00
194_Q198_E0.6601.00
111_V139_G0.6601.00
93_C96_P0.6571.00
47_N50_Y0.6561.00
128_T162_A0.6561.00
22_D28_A0.6551.00
210_H225_Q0.6521.00
152_F212_V0.6511.00
228_M232_K0.6501.00
127_Y176_P0.6501.00
54_I100_A0.6501.00
176_P179_V0.6461.00
15_I54_I0.6431.00
202_N236_H0.6421.00
144_F147_P0.6411.00
37_L86_L0.6411.00
138_P207_L0.6371.00
32_E35_D0.6321.00
137_T210_H0.6291.00
66_G71_L0.6291.00
192_L195_E0.6281.00
40_A44_I0.6281.00
54_I190_V0.6271.00
106_I130_N0.6271.00
121_L125_S0.6261.00
152_F211_L0.6231.00
120_I189_A0.6191.00
99_A119_V0.6171.00
42_E45_R0.6101.00
14_A49_K0.6081.00
52_A98_I0.6061.00
39_Q42_E0.6061.00
25_H67_T0.6061.00
237_E241_S0.6041.00
72_L244_K0.6011.00
143_T222_V0.6011.00
207_L210_H0.6001.00
47_N95_I0.5981.00
98_I193_A0.5981.00
154_L171_E0.5971.00
15_I190_V0.5951.00
120_I185_V0.5951.00
160_L204_L0.5871.00
168_A179_V0.5841.00
129_A142_A0.5811.00
202_N233_T0.5811.00
52_A197_A0.5741.00
120_I188_Y0.5741.00
220_P224_E0.5741.00
157_E169_D0.5741.00
124_E167_G0.5741.00
137_T207_L0.5731.00
136_F239_V0.5731.00
111_V141_G0.5701.00
51_K197_A0.5631.00
184_E191_E0.5621.00
165_Y170_L0.5611.00
150_L155_A0.5611.00
85_N88_S0.5591.00
166_R172_K0.5561.00
239_V242_R0.5541.00
86_L89_L0.5541.00
163_G178_K0.5521.00
9_I43_Y0.5501.00
108_G140_M0.5501.00
132_M199_K0.5491.00
104_H164_S0.5481.00
244_K247_Y0.5481.00
18_V55_L0.5471.00
15_I193_A0.5461.00
154_L172_K0.5451.00
118_I196_L0.5441.00
156_Q208_K0.5431.00
85_N89_L0.5401.00
66_G108_G0.5391.00
41_F89_L0.5391.00
30_S67_T0.5381.00
122_S125_S0.5371.00
34_T38_I0.5371.00
75_Q79_T0.5361.00
113_G116_A0.5361.00
68_Q71_L0.5351.00
149_K177_F0.5351.00
111_V129_A0.5341.00
107_G129_A0.5341.00
129_A159_L0.5331.00
150_L173_R0.5321.00
167_G171_E0.5291.00
139_G142_A0.5291.00
110_F114_L0.5271.00
161_N196_L0.5261.00
129_A146_V0.5261.00
29_F34_T0.5201.00
76_Q79_T0.5191.00
135_G200_P0.5161.00
50_Y53_V0.5161.00
18_V41_F0.5151.00
224_E227_L0.5141.00
111_V145_I0.5141.00
161_N195_E0.5141.00
108_G111_V0.5131.00
29_F108_G0.5121.00
32_E70_G0.5111.00
217_D222_V0.5111.00
105_G110_F0.5101.00
110_F175_V0.5081.00
115_F145_I0.5081.00
41_F97_V0.5071.00
68_Q134_Y0.5061.00
19_K186_L0.5041.00
131_F141_G0.5021.00
138_P159_L0.5011.00
170_L176_P0.5011.00
144_F148_K0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4q1kA 3 1 100 0.064 Contact Map
4jfcA 3 0.9839 100 0.088 Contact Map
3laoA 3 0.9157 100 0.088 Contact Map
4lk5A 3 0.9679 100 0.09 Contact Map
4mi2A 3 0.9839 100 0.092 Contact Map
3swxA 3 0.992 100 0.092 Contact Map
4u19A 3 0.996 100 0.092 Contact Map
1nzyA 4 0.992 100 0.093 Contact Map
2pbpA 5 0.988 100 0.093 Contact Map
4og1A 3 0.9518 100 0.094 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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