GLNK - BACSU - GREMLIN Contacts

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GLNK - Sensor histidine kinase GlnK

UniProt:	P40758	Sequences: MLITVPLAGELKFYPLNEEFRVSFGAPVFFFFLSLLRHVPAVLPGFLTGAAVFIFRVFLELWGGGHNGLTPILYDQASGFFFYMTYACLFSILKANRFRERPIMLGFIGFMIEVVSDCVELTVQFLIFHTVVTPEKITDIAVIAISHTFIVMSFYSVLKLYETQSREKQTRQQHEHMLMIVSNLYEETVHLKKTLKTTEKVTNDSYQLYREMKGKDVQLSGRILRLAGEIHEVKKDNQRIFAGLSKLISNESLRDYMRASDLLQLVIRMNEKYAEALGKQIDFYCSIEGEHDEYHVFIVLSIINNLTANAVEAMDEEGMVSLRLRKPNESMVEFQVEDNGPGISEKIGDIVFDPGFTSKYDEFGTPSTGIGLSYVKEIVTELEGDITFDNQQRGVVFAIRLPVRHLIQKG	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	410 (353)
Sequences:	15074 (13602)
Seq/√Len:	724.0

ECOLI - BARA TORS RCSD RCSC ARCB EVGS BAES GLRK KDPD CUSS CPXA ZRAS ATOS PHOQ YEDV CREC RSTB ENVZ PHOR UHPB QSEC BASS DCUS YPDA DPIB NARX NTRB NARQ
BACSU - GLNK KINB KINE YVRG YCLK KINA YVCQ YYCG BCES CSSS PHOR YCBM RESE KIND YKOH YFIJ YXDK NATK YDFH YXJM DCTS DESK LYTS KINC CITS YVFT MALK YRKQ COMP LIAS YBDK DEGS YHCY
HALSA - Q9HQG5 Q9HQ16 O51970 Q9HRE4 Q9HR10 Q9HRG1 Q9HQ15 Q9HQE9 Q9HNM2 BAT Q9HNA6 Q9HQB9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
209_Y	230_I	3.711	1.00
257_M	294_Y	3.628	1.00
337_E	396_V	3.611	1.00
331_M	400_R	3.264	1.00
387_T	398_A	3.197	1.00
376_K	380_T	3.166	1.00
335_Q	396_V	3.062	1.00
314_M	339_N	3.030	1.00
344_S	347_I	2.920	1.00
348_G	388_F	2.870	1.00
338_D	342_G	2.832	1.00
335_Q	398_A	2.807	1.00
213_K	231_H	2.742	1.00
294_Y	382_L	2.734	1.00
388_F	397_F	2.700	1.00
327_P	333_E	2.698	1.00
256_Y	293_E	2.696	1.00
323_R	335_Q	2.674	1.00
284_Y	323_R	2.662	1.00
333_E	400_R	2.614	1.00
166_R	170_T	2.595	1.00
375_V	386_I	2.542	1.00
348_G	390_N	2.511	1.00
302_I	379_V	2.481	1.00
206_Y	234_K	2.473	1.00
282_D	321_S	2.423	1.00
379_V	384_G	2.379	1.00
282_D	319_M	2.352	1.00
333_E	398_A	2.339	1.00
213_K	228_G	2.226	1.00
323_R	337_E	2.214	1.00
325_R	333_E	2.176	1.00
258_R	261_D	2.163	1.00
302_I	378_I	2.152	1.00
389_D	396_V	2.148	1.00
319_M	339_N	2.137	1.00
284_Y	321_S	2.131	1.00
379_V	399_I	2.123	1.00
325_R	335_Q	2.086	1.00
265_L	300_L	2.054	1.00
254_R	293_E	2.042	1.00
390_N	395_V	2.023	1.00
345_E	348_G	2.010	1.00
177_M	181_V	2.002	1.00
314_M	318_G	1.975	1.00
326_K	403_V	1.966	1.00
318_G	339_N	1.959	1.00
321_S	337_E	1.956	1.00
166_R	169_Q	1.950	1.00
334_F	399_I	1.947	1.00
177_M	180_I	1.925	1.00
302_I	375_V	1.925	1.00
389_D	398_A	1.922	1.00
343_I	347_I	1.902	1.00
255_D	296_V	1.902	1.00
385_D	400_R	1.889	1.00
205_S	234_K	1.860	1.00
352_F	386_I	1.853	1.00
206_Y	235_K	1.852	1.00
343_I	351_V	1.809	1.00
350_I	356_F	1.798	1.00
294_Y	299_V	1.797	1.00
205_S	230_I	1.748	1.00
350_I	353_D	1.745	1.00
347_I	350_I	1.735	1.00
256_Y	291_H	1.730	1.00
219_L	223_I	1.728	1.00
378_I	382_L	1.698	1.00
280_Q	319_M	1.693	1.00
220_S	223_I	1.668	1.00
274_A	311_V	1.618	1.00
212_M	223_I	1.610	1.00
343_I	395_V	1.609	1.00
209_Y	231_H	1.602	1.00
295_H	298_I	1.592	1.00
271_E	275_E	1.586	1.00
270_N	304_N	1.585	1.00
313_A	357_T	1.582	1.00
380_T	385_D	1.581	1.00
212_M	227_A	1.572	1.00
185_Y	189_V	1.569	1.00
170_T	173_Q	1.562	1.00
390_N	393_R	1.549	1.00
173_Q	177_M	1.547	1.00
176_H	179_M	1.527	1.00
347_I	356_F	1.525	1.00
184_L	187_E	1.516	1.00
208_L	226_L	1.510	1.00
308_A	312_E	1.494	1.00
314_M	320_V	1.488	1.00
303_I	334_F	1.474	1.00
176_H	180_I	1.464	1.00
215_K	223_I	1.459	1.00
231_H	235_K	1.458	1.00
213_K	224_L	1.455	1.00
169_Q	173_Q	1.447	1.00
387_T	400_R	1.437	1.00
210_R	231_H	1.425	1.00
298_I	301_S	1.405	1.00
353_D	356_F	1.395	1.00
173_Q	176_H	1.388	1.00
161_Y	165_S	1.372	1.00
338_D	394_G	1.357	1.00
327_P	400_R	1.320	1.00
172_Q	175_E	1.304	1.00
238_Q	242_A	1.300	1.00
243_G	298_I	1.289	1.00
179_M	182_S	1.288	1.00
209_Y	227_A	1.284	1.00
332_V	403_V	1.277	1.00
351_V	388_F	1.272	1.00
170_T	174_H	1.261	1.00
260_S	324_L	1.254	1.00
307_T	322_L	1.243	1.00
352_F	388_F	1.229	1.00
204_D	208_L	1.227	1.00
206_Y	238_Q	1.223	1.00
303_I	307_T	1.209	1.00
298_I	378_I	1.205	1.00
167_E	170_T	1.195	1.00
347_I	358_S	1.193	1.00
285_C	324_L	1.190	1.00
352_F	397_F	1.186	1.00
379_V	401_L	1.164	1.00
197_T	301_S	1.157	1.00
269_M	304_N	1.154	1.00
299_V	382_L	1.146	1.00
171_R	175_E	1.146	1.00
209_Y	213_K	1.142	1.00
305_N	375_V	1.139	1.00
379_V	386_I	1.134	1.00
302_I	399_I	1.126	1.00
310_A	320_V	1.123	1.00
263_L	324_L	1.121	1.00
306_L	399_I	1.120	1.00
260_S	264_Q	1.114	1.00
204_D	233_V	1.106	1.00
75_D	79_G	1.104	1.00
267_I	283_F	1.103	1.00
258_R	291_H	1.095	1.00
175_E	179_M	1.095	1.00
357_T	365_T	1.093	1.00
208_L	229_E	1.091	1.00
312_E	359_K	1.089	1.00
262_L	266_V	1.087	1.00
262_L	294_Y	1.083	1.00
221_G	224_L	1.081	1.00
384_G	401_L	1.078	1.00
321_S	339_N	1.070	1.00
174_H	177_M	1.065	1.00
199_E	242_A	1.051	1.00
175_E	178_L	1.050	1.00
343_I	348_G	1.050	1.00
388_F	395_V	1.049	1.00
266_V	307_T	1.045	1.00
182_S	185_Y	1.038	1.00
157_V	161_Y	1.036	1.00
183_N	187_E	1.033	1.00
209_Y	234_K	1.029	1.00
377_E	380_T	1.021	1.00
331_M	385_D	1.007	1.00
187_E	191_L	1.000	1.00
169_Q	172_Q	1.000	1.00
338_D	395_V	1.000	1.00
265_L	296_V	0.995	1.00
216_D	223_I	0.992	1.00
254_R	382_L	0.990	1.00
198_T	237_N	0.989	1.00
273_Y	276_A	0.987	1.00
216_D	219_L	0.987	1.00
260_S	285_C	0.985	1.00
219_L	222_R	0.985	1.00
174_H	178_L	0.980	1.00
184_L	188_T	0.974	1.00
179_M	183_N	0.973	1.00
229_E	233_V	0.971	1.00
180_I	184_L	0.970	1.00
224_L	228_G	0.969	1.00
259_A	334_F	0.969	1.00
178_L	182_S	0.966	1.00
269_M	300_L	0.965	1.00
228_G	232_E	0.959	1.00
303_I	336_V	0.956	1.00
206_Y	231_H	0.953	1.00
239_R	297_F	0.953	1.00
190_H	194_T	0.950	1.00
351_V	397_F	0.947	1.00
257_M	296_V	0.947	1.00
198_T	241_F	0.947	1.00
286_S	289_G	0.946	1.00
207_Q	211_E	0.944	1.00
270_N	311_V	0.933	1.00
259_A	332_V	0.926	1.00
247_L	373_S	0.923	1.00
167_E	171_R	0.920	1.00
343_I	349_D	0.918	1.00
375_V	379_V	0.916	1.00
322_L	334_F	0.908	1.00
370_I	374_Y	0.907	1.00
233_V	236_D	0.904	1.00
266_V	300_L	0.887	1.00
270_N	307_T	0.887	1.00
306_L	397_F	0.887	1.00
267_I	271_E	0.884	1.00
387_T	399_I	0.882	1.00
343_I	390_N	0.878	1.00
290_E	326_K	0.877	1.00
257_M	262_L	0.873	1.00
343_I	388_F	0.871	1.00
234_K	238_Q	0.871	1.00
310_A	336_V	0.870	1.00
270_N	308_A	0.870	1.00
354_P	377_E	0.867	1.00
313_A	359_K	0.867	1.00
296_V	300_L	0.863	1.00
327_P	331_M	0.860	1.00
337_E	391_Q	0.857	1.00
225_R	229_E	0.856	1.00
289_G	324_L	0.856	1.00
199_E	238_Q	0.856	1.00
313_A	341_P	0.854	1.00
384_G	399_I	0.853	1.00
206_Y	210_R	0.852	1.00
264_Q	268_R	0.849	1.00
193_K	354_P	0.845	1.00
384_G	402_P	0.844	1.00
218_Q	223_I	0.842	1.00
322_L	336_V	0.839	1.00
158_L	161_Y	0.839	1.00
60_E	64_G	0.835	1.00
290_E	332_V	0.835	1.00
267_I	281_I	0.835	1.00
242_A	246_K	0.835	1.00
286_S	325_R	0.834	1.00
357_T	367_S	0.833	1.00
134_P	138_T	0.821	1.00
352_F	376_K	0.818	1.00
263_L	283_F	0.817	1.00
307_T	320_V	0.817	1.00
277_L	311_V	0.815	1.00
279_K	283_F	0.814	1.00
264_Q	283_F	0.814	1.00
56_R	60_E	0.811	1.00
220_S	224_L	0.811	1.00
211_E	226_L	0.809	1.00
194_T	244_L	0.807	1.00
197_T	240_I	0.806	1.00
300_L	304_N	0.804	1.00
249_S	368_T	0.803	1.00
266_V	270_N	0.803	1.00
189_V	354_P	0.802	1.00
279_K	314_M	0.801	1.00
183_N	186_E	0.801	1.00
236_D	300_L	0.799	1.00
171_R	174_H	0.799	1.00
266_V	304_N	0.794	1.00
262_L	299_V	0.794	1.00
181_V	184_L	0.790	1.00
377_E	381_E	0.788	1.00
70_T	74_Y	0.785	1.00
279_K	311_V	0.785	1.00
331_M	402_P	0.785	1.00
205_S	209_Y	0.784	1.00
199_E	245_S	0.782	1.00
168_K	171_R	0.778	1.00
172_Q	176_H	0.775	1.00
79_G	82_F	0.774	1.00
208_L	230_I	0.774	1.00
333_E	387_T	0.769	1.00
324_L	332_V	0.767	1.00
188_T	251_E	0.767	1.00
348_G	352_F	0.765	1.00
168_K	172_Q	0.763	1.00
146_S	150_I	0.762	1.00
324_L	334_F	0.758	1.00
212_M	230_I	0.758	1.00
263_L	322_L	0.757	1.00
274_A	320_V	0.755	1.00
197_T	201_V	0.754	1.00
181_V	185_Y	0.749	1.00
263_L	303_I	0.748	1.00
254_R	294_Y	0.742	1.00
342_G	395_V	0.742	1.00
156_S	160_L	0.740	1.00
348_G	397_F	0.739	1.00
345_E	391_Q	0.732	1.00
330_S	403_V	0.729	1.00
60_E	63_G	0.728	1.00
180_I	183_N	0.727	1.00
160_L	163_T	0.727	1.00
57_V	61_L	0.726	1.00
153_S	156_S	0.723	1.00
267_I	322_L	0.716	1.00
200_K	204_D	0.715	1.00
86_Y	91_S	0.714	1.00
306_L	375_V	0.713	1.00
191_L	195_L	0.712	1.00
159_K	163_T	0.709	1.00
204_D	229_E	0.709	1.00
343_I	397_F	0.708	1.00
72_I	76_Q	0.707	1.00
213_K	227_A	0.707	1.00
243_G	301_S	0.706	1.00
223_I	227_A	0.704	1.00
57_V	60_E	0.702	1.00
301_S	304_N	0.698	1.00
342_G	394_G	0.697	1.00
150_I	157_V	0.695	1.00
313_A	365_T	0.694	1.00
299_V	401_L	0.694	1.00
237_N	240_I	0.691	1.00
228_G	231_H	0.691	1.00
141_A	145_I	0.689	1.00
246_K	301_S	0.688	1.00
262_L	300_L	0.688	1.00
196_K	200_K	0.681	1.00
274_A	281_I	0.681	1.00
232_E	265_L	0.681	1.00
120_E	123_V	0.677	1.00
156_S	159_K	0.677	1.00
159_K	162_E	0.676	1.00
216_D	220_S	0.675	1.00
287_I	290_E	0.675	1.00
223_I	226_L	0.674	1.00
263_L	266_V	0.674	1.00
261_D	265_L	0.673	1.00
162_E	165_S	0.673	1.00
202_T	241_F	0.671	1.00
301_S	378_I	0.669	1.00
268_R	271_E	0.669	1.00
161_Y	164_Q	0.668	1.00
372_L	375_V	0.666	1.00
87_A	91_S	0.665	1.00
182_S	186_E	0.665	1.00
230_I	234_K	0.665	1.00
98_F	101_R	0.664	1.00
136_K	140_I	0.664	1.00
157_V	162_E	0.664	1.00
341_P	365_T	0.664	1.00
72_I	75_D	0.664	1.00
208_L	211_E	0.661	1.00
131_V	135_E	0.661	1.00
386_I	397_F	0.661	1.00
189_V	377_E	0.659	1.00
91_S	95_A	0.659	1.00
267_I	307_T	0.658	1.00
143_I	147_H	0.655	1.00
59_L	63_G	0.651	1.00
57_V	64_G	0.649	1.00
298_I	382_L	0.649	1.00
312_E	357_T	0.649	1.00
237_N	241_F	0.649	1.00
357_T	366_P	0.648	1.00
178_L	181_V	0.646	1.00
226_L	229_E	0.645	1.00
335_Q	389_D	0.642	1.00
199_E	237_N	0.639	1.00
69_L	73_L	0.637	1.00
306_L	379_V	0.637	1.00
373_S	377_E	0.637	1.00
120_E	124_Q	0.637	1.00
247_L	251_E	0.636	1.00
58_F	62_W	0.636	1.00
198_T	244_L	0.635	1.00
200_K	207_Q	0.633	1.00
203_N	207_Q	0.633	1.00
298_I	381_E	0.631	1.00
73_L	77_A	0.630	1.00
203_N	208_L	0.630	1.00
155_Y	159_K	0.630	1.00
180_I	188_T	0.626	1.00
302_I	306_L	0.623	1.00
315_D	318_G	0.619	1.00
69_L	79_G	0.619	1.00
229_E	232_E	0.619	1.00
239_R	243_G	0.619	1.00
102_P	105_L	0.618	1.00
262_L	265_L	0.618	1.00
189_V	193_K	0.617	1.00
306_L	386_I	0.617	1.00
280_Q	283_F	0.617	1.00
361_D	366_P	0.616	1.00
90_F	94_K	0.616	1.00
198_T	201_V	0.614	1.00
351_V	373_S	0.614	1.00
227_A	230_I	0.613	1.00
195_L	201_V	0.612	1.00
169_Q	177_M	0.612	1.00
374_Y	378_I	0.612	1.00
122_T	125_F	0.611	1.00
121_L	124_Q	0.610	1.00
304_N	308_A	0.608	1.00
314_M	317_E	0.604	1.00
51_A	54_I	0.604	1.00
302_I	382_L	0.603	1.00
54_I	58_F	0.602	1.00
75_D	78_S	0.602	1.00
71_P	75_D	0.601	1.00
116_S	120_E	0.599	1.00
49_G	60_E	0.599	1.00
70_T	75_D	0.597	1.00
117_D	120_E	0.595	1.00
345_E	390_N	0.593	1.00
91_S	94_K	0.592	1.00
101_R	105_L	0.588	1.00
61_L	65_G	0.588	1.00
254_R	383_E	0.587	1.00
68_G	71_P	0.586	1.00
309_N	371_G	0.584	1.00
49_G	52_V	0.584	1.00
191_L	194_T	0.582	1.00
136_K	139_D	0.581	1.00
62_W	65_G	0.581	1.00
257_M	299_V	0.580	1.00
253_L	295_H	0.579	1.00
153_S	157_V	0.577	1.00
147_H	151_V	0.574	1.00
152_M	159_K	0.574	1.00
75_D	82_F	0.573	1.00
354_P	373_S	0.573	1.00
119_V	123_V	0.572	1.00
111_M	115_V	0.571	1.00
274_A	279_K	0.571	1.00
286_S	290_E	0.570	1.00
258_R	289_G	0.570	1.00
166_R	173_Q	0.570	1.00
251_E	354_P	0.568	1.00
104_M	107_F	0.565	1.00
326_K	330_S	0.563	1.00
249_S	305_N	0.562	1.00
113_E	117_D	0.562	1.00
351_V	356_F	0.559	1.00
144_A	147_H	0.558	1.00
299_V	303_I	0.557	1.00
74_Y	79_G	0.556	1.00
158_L	162_E	0.552	1.00
203_N	206_Y	0.551	1.00
274_A	277_L	0.551	1.00
264_Q	267_I	0.551	1.00
260_S	289_G	0.550	1.00
288_E	326_K	0.550	1.00
245_S	248_I	0.549	1.00
341_P	395_V	0.549	1.00
160_L	164_Q	0.548	1.00
61_L	66_H	0.548	1.00
123_V	126_L	0.548	1.00
55_F	58_F	0.548	1.00
156_S	163_T	0.547	1.00
244_L	248_I	0.547	1.00
231_H	234_K	0.546	1.00
215_K	219_L	0.546	1.00
139_D	143_I	0.545	1.00
306_L	336_V	0.545	1.00
361_D	365_T	0.545	1.00
55_F	60_E	0.543	1.00
149_F	152_M	0.542	1.00
125_F	128_F	0.542	1.00
297_F	300_L	0.542	1.00
215_K	220_S	0.541	1.00
272_K	276_A	0.540	1.00
152_M	156_S	0.540	1.00
399_I	402_P	0.540	1.00
379_V	385_D	0.539	1.00
334_F	401_L	0.538	1.00
92_I	95_A	0.538	1.00
212_M	226_L	0.537	1.00
76_Q	79_G	0.536	1.00
210_R	214_G	0.536	1.00
74_Y	78_S	0.536	1.00
286_S	324_L	0.535	1.00
338_D	341_P	0.534	1.00
216_D	224_L	0.533	1.00
208_L	233_V	0.533	1.00
281_I	322_L	0.533	1.00
274_A	278_G	0.531	1.00
62_W	66_H	0.531	1.00
148_T	151_V	0.531	1.00
148_T	152_M	0.531	1.00
221_G	225_R	0.530	1.00
339_N	393_R	0.530	1.00
195_L	199_E	0.529	1.00
250_N	377_E	0.528	1.00
355_G	370_I	0.528	1.00
63_G	67_N	0.527	1.00
307_T	311_V	0.527	1.00
101_R	104_M	0.526	1.00
190_H	193_K	0.526	1.00
94_K	98_F	0.526	1.00
245_S	249_S	0.525	1.00
359_K	365_T	0.523	1.00
222_R	226_L	0.523	1.00
69_L	72_I	0.521	1.00
151_V	155_Y	0.520	1.00
85_T	88_C	0.520	1.00
245_S	368_T	0.519	1.00
197_T	200_K	0.518	1.00
266_V	269_M	0.517	1.00
88_C	91_S	0.517	1.00
203_N	233_V	0.515	1.00
204_D	207_Q	0.515	1.00
112_I	116_S	0.515	1.00
295_H	382_L	0.515	1.00
131_V	134_P	0.515	1.00
52_V	60_E	0.515	1.00
193_K	197_T	0.515	1.00
140_I	144_A	0.514	1.00
71_P	74_Y	0.513	1.00
60_E	65_G	0.512	1.00
148_T	156_S	0.511	1.00
225_R	228_G	0.510	1.00
270_N	273_Y	0.509	1.00
238_Q	241_F	0.509	1.00
379_V	382_L	0.509	1.00
330_S	402_P	0.508	1.00
123_V	127_I	0.507	1.00
285_C	289_G	0.507	1.00
122_T	127_I	0.506	1.00
120_E	125_F	0.506	1.00
311_V	320_V	0.506	1.00
235_K	238_Q	0.505	0.99
102_P	106_G	0.505	0.99
57_V	62_W	0.504	0.99
62_W	67_N	0.504	0.99
86_Y	89_L	0.504	0.99
236_D	240_I	0.503	0.99
340_G	395_V	0.502	0.99
284_Y	325_R	0.502	0.99
52_V	59_L	0.500	0.99
97_R	100_E	0.500	0.99
119_V	125_F	0.500	0.99

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4i5sA	2	0.6537	100	0.425	Contact Map
4q20A	2	0.5488	100	0.436	Contact Map
3d36A	2	0.5341	100	0.449	Contact Map
3jz3A	2	0.3805	100	0.45	Contact Map
3a0rA	2	0.8049	100	0.452	Contact Map
2c2aA	2	0.5585	100	0.454	Contact Map
4bixA	2	0.5463	100	0.455	Contact Map
4mpcA	2	0.7049	100	0.457	Contact Map
2q8gA	2	0.7439	100	0.468	Contact Map
4gczA	2	0.8829	100	0.473	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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