RSBU - BACSU - GREMLIN Contacts

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RSBU - Phosphoserine phosphatase RsbU

UniProt:	P40399	Sequences: MDFREVIEQRYHQLLSRYIAELTETSLYQAQKFSRKTIEHQIPPEEIISIHRKVLKELYPSLPEDVFHSLDFLIEVMIGYGMAYQEHQTLRGIQQEIKSEIEIAANVQQTLLGTKVPQEEALDIGAISVPAKQMSGDYYHFVKDKESINIAIADVIGKGIPAALCMSMIKYAMDSLPETGIHPSQVLKNLNRVVEQNVDASMFITMFYANYNMDKHQFTYASAGHEPGFYYSQKDNTFYDLEAKGLVLGISQDYDYKQFDQHLEKGDMIVLFSDGVTECRTENGFLERPDLQKLIEEHMCSSAQEMVKNIYDSLLKLQDFQLHDDFTLIVLRRKV	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	335 (262)
Sequences:	6684 (4467)
Seq/√Len:	275.9

BACSU - RSBU RSBP

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
217_Q	260_D	4.259	1.00
182_H	258_Q	3.409	1.00
268_M	332_R	3.361	1.00
184_S	258_Q	3.347	1.00
286_L	313_S	3.345	1.00
264_E	267_D	3.332	1.00
187_L	221_A	3.288	1.00
149_N	208_Y	3.034	1.00
232_S	239_Y	2.962	1.00
232_S	267_D	2.901	1.00
219_T	260_D	2.830	1.00
270_V	330_V	2.820	1.00
217_Q	262_H	2.810	1.00
147_S	212_N	2.648	1.00
219_T	258_Q	2.573	1.00
233_Q	267_D	2.437	1.00
169_I	204_I	2.375	1.00
123_D	332_R	2.372	1.00
147_S	210_N	2.317	1.00
286_L	290_D	2.220	1.00
229_F	240_D	2.182	1.00
76_V	79_G	2.074	1.00
205_T	225_H	2.065	1.00
126_A	139_Y	2.064	1.00
183_P	221_A	2.047	1.00
240_D	292_Q	2.004	1.00
76_V	80_Y	1.976	1.00
149_N	210_N	1.860	1.00
150_I	209_A	1.847	1.00
150_I	331_L	1.804	1.00
146_E	214_D	1.801	1.00
83_A	86_E	1.791	1.00
209_A	218_F	1.787	1.00
152_I	273_S	1.744	1.00
303_A	330_V	1.721	1.00
302_S	305_E	1.710	1.00
87_H	90_L	1.704	1.00
69_S	72_F	1.704	1.00
207_F	220_Y	1.630	1.00
129_V	315_L	1.617	1.00
307_V	330_V	1.591	1.00
240_D	288_R	1.589	1.00
232_S	235_D	1.557	1.00
294_L	313_S	1.546	1.00
322_L	326_F	1.545	1.00
144_D	147_S	1.532	1.00
127_I	304_Q	1.494	1.00
301_S	305_E	1.491	1.00
218_F	261_Q	1.483	1.00
72_F	76_V	1.478	1.00
82_M	86_E	1.470	1.00
218_F	269_I	1.470	1.00
80_Y	83_A	1.470	1.00
79_G	83_A	1.446	1.00
105_A	109_Q	1.443	1.00
140_H	174_D	1.436	1.00
270_V	295_I	1.434	1.00
297_E	313_S	1.430	1.00
206_M	248_L	1.429	1.00
216_H	333_R	1.420	1.00
298_H	309_N	1.411	1.00
205_T	247_V	1.392	1.00
177_P	186_V	1.391	1.00
220_Y	241_L	1.378	1.00
293_K	297_E	1.375	1.00
308_K	312_D	1.353	1.00
241_L	261_Q	1.353	1.00
73_L	76_V	1.349	1.00
229_F	292_Q	1.334	1.00
69_S	73_L	1.332	1.00
229_F	238_F	1.319	1.00
212_N	215_K	1.312	1.00
92_G	95_Q	1.303	1.00
270_V	328_L	1.303	1.00
172_A	193_V	1.295	1.00
298_H	306_M	1.295	1.00
314_L	326_F	1.293	1.00
215_K	262_H	1.292	1.00
123_D	303_A	1.279	1.00
151_A	173_M	1.275	1.00
179_T	189_N	1.274	1.00
301_S	309_N	1.261	1.00
232_S	264_E	1.259	1.00
177_P	208_Y	1.255	1.00
282_E	290_D	1.249	1.00
207_F	222_S	1.241	1.00
191_N	251_S	1.229	1.00
127_I	308_K	1.208	1.00
222_S	244_K	1.204	1.00
152_I	327_T	1.203	1.00
94_Q	98_K	1.196	1.00
280_R	323_H	1.179	1.00
220_Y	259_F	1.174	1.00
191_N	195_E	1.173	1.00
183_P	208_Y	1.172	1.00
132_K	158_K	1.167	1.00
111_L	167_S	1.165	1.00
280_R	285_F	1.162	1.00
185_Q	188_K	1.159	1.00
127_I	311_Y	1.156	1.00
287_E	290_D	1.154	1.00
80_Y	84_Y	1.153	1.00
156_I	203_F	1.137	1.00
209_A	271_L	1.129	1.00
98_K	133_Q	1.123	1.00
278_E	285_F	1.117	1.00
294_L	309_N	1.114	1.00
268_M	330_V	1.108	1.00
268_M	303_A	1.107	1.00
192_R	253_D	1.096	1.00
86_E	89_T	1.093	1.00
173_M	190_L	1.089	1.00
239_Y	261_Q	1.085	1.00
76_V	83_A	1.081	1.00
124_I	141_F	1.071	1.00
137_D	274_D	1.067	1.00
238_F	292_Q	1.063	1.00
294_L	298_H	1.058	1.00
77_M	80_Y	1.055	1.00
170_K	174_D	1.053	1.00
84_Y	88_Q	1.051	1.00
186_V	208_Y	1.043	1.00
294_L	310_I	1.041	1.00
73_L	79_G	1.032	1.00
80_Y	86_E	1.030	1.00
309_N	313_S	1.030	1.00
207_F	228_G	1.026	1.00
79_G	87_H	1.022	1.00
277_T	291_L	1.018	1.00
187_L	206_M	1.018	1.00
146_E	213_M	1.017	1.00
154_D	278_E	1.013	1.00
277_T	288_R	1.004	1.00
220_Y	261_Q	1.002	1.00
125_G	330_V	1.002	1.00
274_D	324_D	1.001	1.00
232_S	237_T	0.999	1.00
72_F	79_G	0.995	1.00
146_E	212_N	0.994	1.00
154_D	324_D	0.990	1.00
89_T	93_I	0.986	1.00
152_I	329_I	0.981	1.00
191_N	256_Y	0.979	1.00
129_V	311_Y	0.976	1.00
191_N	253_D	0.968	1.00
191_N	249_G	0.961	1.00
308_K	311_Y	0.961	1.00
140_H	170_K	0.960	1.00
80_Y	87_H	0.956	1.00
78_I	81_G	0.956	1.00
182_H	185_Q	0.954	1.00
83_A	87_H	0.948	1.00
289_P	293_K	0.946	1.00
149_N	186_V	0.943	1.00
90_L	94_Q	0.940	1.00
150_I	329_I	0.936	1.00
125_G	307_V	0.935	1.00
82_M	85_Q	0.931	1.00
92_G	96_E	0.931	1.00
153_A	206_M	0.926	1.00
203_F	250_I	0.919	1.00
274_D	278_E	0.917	1.00
176_L	186_V	0.917	1.00
231_Y	238_F	0.915	1.00
238_F	296_E	0.914	1.00
151_A	208_Y	0.910	1.00
183_P	258_Q	0.906	1.00
172_A	175_S	0.905	1.00
188_K	255_D	0.901	1.00
93_I	96_E	0.900	1.00
305_E	309_N	0.898	1.00
269_I	331_L	0.893	1.00
286_L	294_L	0.892	1.00
154_D	158_K	0.888	1.00
150_I	218_F	0.874	1.00
152_I	207_F	0.869	1.00
209_A	220_Y	0.861	1.00
99_S	102_E	0.858	1.00
292_Q	296_E	0.857	1.00
154_D	274_D	0.856	1.00
108_Q	166_M	0.850	1.00
155_V	225_H	0.841	1.00
223_A	256_Y	0.840	1.00
93_I	97_I	0.836	1.00
137_D	154_D	0.835	1.00
183_P	187_L	0.834	1.00
304_Q	308_K	0.831	1.00
290_D	293_K	0.820	0.99
148_I	213_M	0.816	0.99
79_G	84_Y	0.815	0.99
76_V	87_H	0.815	0.99
272_F	277_T	0.809	0.99
153_A	204_I	0.809	0.99
144_D	213_M	0.803	0.99
230_Y	267_D	0.803	0.99
276_V	326_F	0.801	0.99
166_M	171_Y	0.798	0.99
122_L	333_R	0.797	0.99
176_L	190_L	0.794	0.99
127_I	307_V	0.787	0.99
245_G	256_Y	0.786	0.99
75_E	79_G	0.783	0.99
98_K	102_E	0.779	0.99
293_K	296_E	0.778	0.99
211_Y	216_H	0.777	0.99
303_A	332_R	0.776	0.99
69_S	75_E	0.775	0.99
81_G	85_Q	0.772	0.99
230_Y	241_L	0.771	0.99
263_L	333_R	0.771	0.99
191_N	250_I	0.766	0.99
211_Y	218_F	0.763	0.99
138_Y	153_A	0.759	0.99
125_G	303_A	0.758	0.99
216_H	265_K	0.750	0.99
74_I	78_I	0.749	0.99
123_D	302_S	0.748	0.99
273_S	327_T	0.747	0.99
79_G	86_E	0.746	0.99
272_F	276_V	0.743	0.99
81_G	84_Y	0.741	0.99
268_M	306_M	0.740	0.99
116_V	141_F	0.740	0.99
237_T	296_E	0.739	0.99
276_V	314_L	0.733	0.99
169_I	194_V	0.731	0.99
246_L	254_Y	0.727	0.99
222_S	225_H	0.725	0.99
158_K	274_D	0.724	0.99
76_V	84_Y	0.722	0.99
222_S	228_G	0.721	0.99
291_L	310_I	0.721	0.99
309_N	312_D	0.721	0.99
86_E	90_L	0.719	0.99
94_Q	97_I	0.718	0.99
231_Y	295_I	0.718	0.99
295_I	306_M	0.717	0.99
281_T	284_G	0.714	0.99
279_C	282_E	0.714	0.99
184_S	188_K	0.712	0.98
307_V	328_L	0.710	0.98
138_Y	206_M	0.706	0.98
153_A	169_I	0.705	0.98
137_D	325_D	0.705	0.98
69_S	79_G	0.696	0.98
224_G	228_G	0.694	0.98
138_Y	166_M	0.693	0.98
230_Y	269_I	0.692	0.98
125_G	304_Q	0.691	0.98
272_F	291_L	0.689	0.98
104_A	134_M	0.687	0.98
151_A	206_M	0.687	0.98
87_H	91_R	0.682	0.98
226_E	272_F	0.682	0.98
173_M	206_M	0.681	0.98
70_L	73_L	0.681	0.98
158_K	204_I	0.679	0.98
305_E	308_K	0.678	0.98
123_D	304_Q	0.675	0.98
276_V	328_L	0.675	0.98
90_L	158_K	0.675	0.98
143_K	148_I	0.671	0.98
244_K	257_K	0.671	0.98
164_L	167_S	0.669	0.98
210_N	219_T	0.669	0.98
247_V	250_I	0.668	0.98
74_I	81_G	0.667	0.98
230_Y	263_L	0.666	0.98
279_C	314_L	0.664	0.98
245_G	257_K	0.664	0.98
229_F	295_I	0.663	0.98
298_H	301_S	0.662	0.98
116_V	126_A	0.662	0.98
170_K	197_N	0.661	0.98
85_Q	89_T	0.660	0.98
294_L	297_E	0.659	0.98
212_N	217_Q	0.657	0.97
225_H	228_G	0.656	0.97
141_F	148_I	0.654	0.97
158_K	278_E	0.653	0.97
80_Y	91_R	0.652	0.97
124_I	331_L	0.651	0.97
191_N	252_Q	0.649	0.97
155_V	166_M	0.646	0.97
138_Y	204_I	0.646	0.97
231_Y	299_M	0.644	0.97
279_C	286_L	0.642	0.97
226_E	277_T	0.641	0.97
69_S	76_V	0.641	0.97
246_L	250_I	0.639	0.97
80_Y	90_L	0.638	0.97
107_V	164_L	0.637	0.97
221_A	245_G	0.636	0.97
280_R	284_G	0.635	0.97
184_S	221_A	0.634	0.97
226_E	288_R	0.634	0.97
233_Q	266_G	0.631	0.97
175_S	193_V	0.629	0.97
315_L	319_D	0.629	0.97
76_V	81_G	0.627	0.97
147_S	219_T	0.627	0.97
89_T	92_G	0.627	0.97
158_K	162_A	0.626	0.97
230_Y	239_Y	0.626	0.97
143_K	213_M	0.623	0.97
113_G	140_H	0.620	0.96
108_Q	112_L	0.619	0.96
137_D	324_D	0.619	0.96
172_A	176_L	0.612	0.96
228_G	241_L	0.612	0.96
73_L	83_A	0.610	0.96
161_P	202_M	0.610	0.96
240_D	295_I	0.609	0.96
160_I	164_L	0.609	0.96
152_I	271_L	0.606	0.96
128_S	327_T	0.604	0.96
72_F	77_M	0.601	0.96
231_Y	268_M	0.596	0.96
158_K	324_D	0.595	0.96
141_F	329_I	0.590	0.95
75_E	83_A	0.588	0.95
142_V	177_P	0.585	0.95
288_R	292_Q	0.584	0.95
189_N	192_R	0.582	0.95
122_L	211_Y	0.582	0.95
263_L	267_D	0.581	0.95
216_H	262_H	0.581	0.95
73_L	77_M	0.579	0.95
282_E	318_Q	0.578	0.95
188_K	192_R	0.578	0.95
251_S	254_Y	0.576	0.95
72_F	89_T	0.575	0.95
216_H	263_L	0.572	0.94
72_F	80_Y	0.571	0.94
243_A	259_F	0.570	0.94
264_E	333_R	0.570	0.94
119_E	148_I	0.565	0.94
225_H	272_F	0.562	0.94
153_A	166_M	0.560	0.94
233_Q	264_E	0.558	0.94
71_D	74_I	0.557	0.94
77_M	83_A	0.554	0.93
225_H	273_S	0.553	0.93
270_V	310_I	0.553	0.93
104_A	108_Q	0.551	0.93
221_A	258_Q	0.547	0.93
167_S	171_Y	0.546	0.93
169_I	190_L	0.544	0.93
108_Q	134_M	0.544	0.93
140_H	151_A	0.542	0.93
166_M	204_I	0.542	0.93
232_S	238_F	0.540	0.93
124_I	150_I	0.540	0.93
76_V	91_R	0.539	0.93
144_D	210_N	0.537	0.92
131_A	322_L	0.537	0.92
72_F	75_E	0.536	0.92
79_G	82_M	0.536	0.92
117_P	141_F	0.535	0.92
69_S	77_M	0.532	0.92
315_L	322_L	0.531	0.92
270_V	307_V	0.531	0.92
173_M	186_V	0.528	0.92
152_I	166_M	0.527	0.92
93_I	98_K	0.526	0.92
192_R	254_Y	0.524	0.91
241_L	269_I	0.523	0.91
277_T	285_F	0.522	0.91
225_H	278_E	0.521	0.91
211_Y	263_L	0.520	0.91
236_N	296_E	0.520	0.91
138_Y	170_K	0.520	0.91
76_V	86_E	0.519	0.91
111_L	170_K	0.516	0.91
104_A	164_L	0.514	0.91
158_K	323_H	0.514	0.91
75_E	80_Y	0.513	0.91
97_I	101_I	0.512	0.91
91_R	95_Q	0.512	0.91
95_Q	98_K	0.512	0.91
84_Y	87_H	0.512	0.91
101_I	105_A	0.512	0.91
156_I	198_V	0.509	0.90
159_G	202_M	0.508	0.90
72_F	83_A	0.508	0.90
154_D	205_T	0.508	0.90
209_A	331_L	0.505	0.90
244_K	247_V	0.505	0.90
238_F	295_I	0.505	0.90
276_V	310_I	0.502	0.90
176_L	179_T	0.502	0.90
111_L	164_L	0.502	0.90
289_P	292_Q	0.500	0.89

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3eq2A	2	0.9313	100	0.375	Contact Map
3ke6A	2	0.6657	100	0.382	Contact Map
3f79A	2	0.7015	100	0.393	Contact Map
3pu9A	2	0.6478	100	0.493	Contact Map
3t91A	3	0.6358	100	0.509	Contact Map
3zt9A	1	0.5672	100	0.549	Contact Map
3w42A	1	0.5881	100	0.567	Contact Map
2j82A	1	0.5761	100	0.633	Contact Map
2jfrA	1	0.5821	100	0.637	Contact Map
3rnrA	2	0.5403	100	0.646	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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