GREMLIN Database
NPRB - Neutral protease B
UniProt: P39899 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 538 (466)
Sequences: 1690 (1220)
Seq/√Len: 56.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
376_T396_S5.3461.00
484_A524_Q5.2061.00
177_Y188_A4.8541.00
497_V501_T3.9151.00
188_A215_I3.9131.00
511_I525_S3.7661.00
497_V509_A3.7531.00
391_L506_M3.7251.00
389_G452_V3.5841.00
260_T301_H3.4671.00
310_Y315_F3.4661.00
98_F118_A3.4351.00
311_Y372_T3.2301.00
380_A493_L3.1791.00
176_L187_L3.1571.00
402_M486_E3.1001.00
394_S459_H3.0311.00
496_Y516_D3.0011.00
230_G298_V2.7881.00
484_A518_Y2.7851.00
120_K124_Q2.7481.00
429_D432_K2.7441.00
315_F487_Q2.7271.00
383_L500_S2.6991.00
186_D208_D2.6521.00
401_A410_I2.6311.00
109_P175_E2.5561.00
390_A455_N2.5361.00
175_E190_L2.5271.00
510_A532_Y2.4981.00
458_I535_V2.4851.00
332_H351_D2.4761.00
320_F374_A2.3511.00
310_Y486_E2.3431.00
325_A374_A2.3231.00
143_P187_L2.3201.00
368_A400_G2.2981.00
437_D444_T2.2641.00
502_D505_M2.2571.00
114_E499_A2.2221.00
337_W341_A2.1711.00
202_Y343_N2.1681.00
417_P422_D2.1471.00
329_S349_Y2.1311.00
207_I214_V2.1241.00
444_T458_I2.0921.00
304_A329_S2.0831.00
366_I414_V2.0711.00
463_A485_S2.0461.00
145_I189_Y2.0421.00
190_L204_H2.0301.00
491_R516_D2.0141.00
344_G377_Q2.0071.00
398_I489_Y2.0011.00
416_T422_D2.0001.00
512_E516_D1.9871.00
511_I529_E1.9851.00
311_Y375_V1.9441.00
150_A154_A1.9341.00
333_V337_W1.8971.00
491_R495_Y1.8581.00
341_A348_V1.8570.99
330_T346_Q1.8550.99
300_A331_V1.8260.99
384_Y456_S1.7940.99
191_V207_I1.7620.99
320_F325_A1.7590.99
85_V99_F1.7540.99
80_N106_K1.7070.99
201_G219_N1.7070.99
466_L528_V1.7020.99
230_G233_G1.6920.99
377_Q381_G1.6850.99
330_T348_V1.6830.99
146_S149_K1.6760.99
204_H220_A1.6720.99
262_D293_N1.6660.99
518_Y524_Q1.6550.99
444_T447_E1.6500.99
179_Y188_A1.6130.99
208_D215_I1.6120.99
85_V101_L1.5990.98
208_D211_N1.5860.98
342_W377_Q1.5840.98
388_P452_V1.5430.98
515_N525_S1.5310.98
368_A403_A1.5250.98
143_P176_L1.5190.98
117_V125_V1.5170.98
230_G234_D1.5140.98
471_V474_G1.5130.98
216_E385_Q1.5110.98
409_E432_K1.5040.98
426_S432_K1.4580.97
307_V311_Y1.4480.97
442_R445_G1.4430.97
90_K97_T1.4410.97
111_Y177_Y1.4380.97
399_M493_L1.4350.97
470_G477_V1.4350.97
433_Q436_P1.4230.97
315_F490_Y1.4130.97
91_D98_F1.3940.96
459_H532_Y1.3940.96
242_E245_G1.3850.96
241_T248_Y1.3800.96
230_G236_K1.3720.96
338_N353_D1.3580.96
83_F101_L1.3560.96
428_E432_K1.3560.96
437_D440_S1.3500.96
311_Y399_M1.3480.96
91_D491_R1.3460.96
144_S210_K1.3430.95
379_S494_T1.3420.95
472_H536_G1.3370.95
238_F249_L1.3360.95
470_G475_V1.3310.95
242_E246_R1.3270.95
491_R517_L1.3270.95
523_K527_S1.3190.95
118_A496_Y1.3170.95
480_I484_A1.3170.95
459_H503_F1.3160.95
526_A529_E1.3040.95
457_S460_N1.3020.95
280_Y285_I1.3010.95
462_A466_L1.2860.94
532_Y537_I1.2810.94
525_S529_E1.2690.94
308_Y319_S1.2680.94
501_T505_M1.2660.94
140_S210_K1.2650.94
456_S460_N1.2640.94
526_A530_K1.2630.94
229_I235_E1.2520.93
118_A129_F1.2520.93
280_Y294_E1.2500.93
462_A528_V1.2490.93
192_R202_Y1.2430.93
251_D254_R1.2420.93
398_I463_A1.2360.93
507_K533_E1.2270.93
437_D447_E1.2230.93
63_F101_L1.2230.93
338_N357_F1.2220.93
99_F119_M1.2000.92
157_E205_Y1.1970.92
399_M486_E1.1970.92
180_P185_Y1.1920.92
100_K116_A1.1840.91
333_V341_A1.1780.91
240_V247_F1.1720.91
376_T493_L1.1710.91
514_A524_Q1.1680.91
439_Y442_R1.1650.91
297_A331_V1.1630.91
473_H476_Q1.1600.90
492_A497_V1.1600.90
381_G499_A1.1600.90
372_T399_M1.1590.90
337_W351_D1.1540.90
394_S398_I1.1520.90
46_E49_P1.1520.90
370_E373_H1.1510.90
192_R204_H1.1390.90
193_L205_Y1.1330.89
230_G299_D1.1310.89
436_P440_S1.1260.89
315_F486_E1.1240.89
111_Y206_F1.1230.89
426_S433_Q1.1190.89
282_G285_I1.1180.89
437_D441_N1.1130.89
321_D378_Y1.1100.88
318_D321_D1.1080.88
439_Y472_H1.1070.88
296_A359_P1.1070.88
496_Y509_A1.1070.88
435_N457_S1.1040.88
314_T402_M1.1030.88
387_E439_Y1.1010.88
347_M360_L1.0940.88
227_T230_G1.0920.88
406_D482_R1.0920.88
228_G298_V1.0900.87
318_D324_G1.0870.87
339_N453_H1.0870.87
304_A349_Y1.0850.87
496_Y513_A1.0800.87
401_A407_D1.0800.87
179_Y206_F1.0780.87
176_L180_P1.0750.87
300_A329_S1.0740.87
471_V475_V1.0730.87
247_F251_D1.0700.87
226_G240_V1.0700.87
153_T156_R1.0690.86
57_T71_T1.0660.86
149_K152_H1.0650.86
237_S240_V1.0650.86
187_L209_A1.0640.86
120_K126_K1.0630.86
321_D325_A1.0560.86
314_T483_E1.0500.85
157_E217_S1.0500.85
392_N396_S1.0400.85
280_Y284_E1.0360.85
301_H305_Q1.0320.84
339_N452_V1.0300.84
368_A401_A1.0300.84
437_D443_Y1.0260.84
240_V243_Q1.0260.84
65_Y69_H1.0260.84
310_Y314_T1.0260.84
496_Y512_E1.0240.84
367_V371_I1.0220.84
267_N333_V1.0220.84
94_T491_R1.0190.84
214_V217_S1.0170.84
401_A404_D1.0150.83
221_I343_N1.0100.83
243_Q248_Y1.0100.83
529_E533_E1.0060.83
524_Q527_S1.0020.83
391_L503_F1.0020.83
410_I424_L0.9940.82
380_A497_V0.9930.82
227_T235_E0.9930.82
492_A510_A0.9880.82
512_E515_N0.9860.82
461_K465_L0.9830.81
108_I187_L0.9810.81
241_T252_E0.9790.81
384_Y460_N0.9750.81
466_L485_S0.9750.81
347_M371_I0.9750.81
430_P535_V0.9730.81
437_D458_I0.9730.81
174_G191_V0.9710.81
333_V348_V0.9710.81
330_T333_V0.9700.81
338_N416_T0.9660.80
465_L535_V0.9640.80
409_E426_S0.9640.80
267_N334_G0.9610.80
530_K533_E0.9610.80
386_G447_E0.9590.80
389_G411_G0.9580.80
472_H475_V0.9560.80
374_A378_Y0.9560.80
395_I489_Y0.9540.79
387_E452_V0.9530.79
340_A411_G0.9510.79
398_I430_P0.9470.79
310_Y399_M0.9450.79
243_Q247_F0.9430.79
398_I459_H0.9420.79
243_Q246_R0.9400.78
431_S441_N0.9280.78
415_Y422_D0.9280.78
151_I172_P0.9260.77
84_R104_V0.9260.77
202_Y220_A0.9250.77
528_V537_I0.9230.77
409_E412_E0.9170.77
206_F218_F0.9150.77
118_A516_D0.9150.77
510_A528_V0.9140.76
508_Q512_E0.9140.76
440_S443_Y0.9120.76
179_Y215_I0.9100.76
466_L531_A0.9100.76
82_H104_V0.9070.76
287_S291_V0.9070.76
376_T392_N0.9060.76
329_S367_V0.9020.76
389_G456_S0.9020.76
391_L501_T0.9020.76
176_L209_A0.9010.75
402_M482_R0.9000.75
507_K511_I0.8980.75
230_G235_E0.8980.75
489_Y531_A0.8960.75
382_L388_P0.8910.75
492_A509_A0.8900.75
463_A467_A0.8870.74
128_F134_P0.8810.74
145_I212_G0.8810.74
465_L470_G0.8770.73
436_P439_Y0.8760.73
314_T487_Q0.8740.73
145_I153_T0.8710.73
392_N456_S0.8700.73
423_S426_S0.8700.73
317_R490_Y0.8680.73
377_Q392_N0.8670.73
465_L534_A0.8660.72
241_T250_A0.8650.72
402_M463_A0.8630.72
300_A361_S0.8620.72
480_I524_Q0.8620.72
443_Y446_T0.8580.72
322_Q490_Y0.8560.72
149_K153_T0.8560.72
125_V128_F0.8550.72
468_E482_R0.8550.72
438_H536_G0.8520.71
384_Y389_G0.8520.71
248_Y279_G0.8480.71
240_V245_G0.8470.71
469_G527_S0.8460.71
458_I503_F0.8440.71
86_S102_T0.8330.70
394_S532_Y0.8310.69
289_T292_F0.8310.69
230_G295_P0.8270.69
108_I178_I0.8240.69
255_G261_F0.8230.69
338_N415_Y0.8210.68
228_G231_V0.8190.68
329_S371_I0.8180.68
469_G534_A0.8150.68
111_Y383_L0.8140.68
436_P455_N0.8080.67
458_I462_A0.8080.67
416_T423_S0.8050.67
245_G251_D0.8020.67
229_I233_G0.8010.67
49_P54_Q0.8010.67
384_Y388_P0.8000.66
314_T486_E0.7980.66
402_M467_A0.7980.66
347_M374_A0.7970.66
141_V178_I0.7970.66
427_L460_N0.7950.66
154_A205_Y0.7950.66
376_T379_S0.7940.66
372_T395_I0.7930.66
409_E423_S0.7930.66
415_Y423_S0.7920.66
436_P443_Y0.7920.66
389_G392_N0.7900.65
318_D322_Q0.7900.65
280_Y283_K0.7870.65
431_S461_K0.7870.65
309_D313_K0.7860.65
314_T322_Q0.7840.65
410_I427_L0.7820.65
382_L456_S0.7810.65
303_N367_V0.7810.65
178_I185_Y0.7780.64
158_L193_L0.7770.64
505_M508_Q0.7770.64
398_I464_Y0.7760.64
48_S54_Q0.7710.64
387_E442_R0.7710.64
241_T245_G0.7700.63
49_P68_K0.7690.63
338_N360_L0.7680.63
471_V476_Q0.7680.63
310_Y487_Q0.7680.63
366_I424_L0.7660.63
384_Y452_V0.7650.63
279_G282_G0.7640.63
507_K532_Y0.7540.62
488_I524_Q0.7530.62
296_A299_D0.7500.61
153_T157_E0.7500.61
145_I149_K0.7500.61
254_R257_G0.7490.61
239_D258_I0.7470.61
229_I298_V0.7470.61
76_K81_S0.7460.61
459_H510_A0.7440.61
382_L460_N0.7430.61
337_W348_V0.7400.60
375_V399_M0.7390.60
55_N59_K0.7390.60
232_S235_E0.7380.60
260_T297_A0.7360.60
202_Y377_Q0.7340.60
115_Q128_F0.7340.60
228_G232_S0.7320.60
441_N444_T0.7320.60
411_G414_V0.7310.59
360_L424_L0.7290.59
242_E248_Y0.7260.59
488_I514_A0.7260.59
483_E486_E0.7250.59
202_Y221_I0.7230.59
439_Y443_Y0.7230.59
58_Q61_S0.7230.59
411_G452_V0.7220.58
186_D213_K0.7210.58
491_R513_A0.7210.58
253_T257_G0.7210.58
409_E428_E0.7190.58
350_G360_L0.7190.58
238_F243_Q0.7190.58
263_A269_T0.7170.58
342_W374_A0.7140.58
105_Y110_I0.7140.58
254_R309_D0.7130.58
171_E174_G0.7130.58
364_L404_D0.7110.57
357_F424_L0.7100.57
393_E397_D0.7070.57
392_N493_L0.7040.57
462_A535_V0.7020.56
484_A488_I0.7020.56
56_T60_E0.7020.56
240_V251_D0.7010.56
356_K415_Y0.6990.56
53_V61_S0.6990.56
197_E200_P0.6980.56
262_D265_N0.6960.56
69_H73_F0.6960.56
267_N337_W0.6940.55
236_K240_V0.6940.55
238_F242_E0.6920.55
80_N152_H0.6910.55
154_A193_L0.6880.55
296_A361_S0.6860.55
56_T64_S0.6840.54
226_G230_G0.6830.54
57_T60_E0.6810.54
463_A535_V0.6800.54
447_E454_I0.6790.54
438_H442_R0.6780.54
357_F415_Y0.6770.54
130_G536_G0.6760.54
399_M490_Y0.6750.53
436_P454_I0.6740.53
106_K178_I0.6730.53
306_A363_S0.6730.53
117_V150_A0.6730.53
176_L189_Y0.6730.53
177_Y215_I0.6720.53
319_S325_A0.6720.53
364_L423_S0.6710.53
260_T349_Y0.6700.53
174_G513_A0.6690.53
440_S447_E0.6690.53
141_V187_L0.6690.53
53_V56_T0.6680.53
247_F488_I0.6680.53
338_N356_K0.6660.52
349_Y371_I0.6660.52
372_T375_V0.6650.52
241_T253_T0.6630.52
228_G277_L0.6620.52
116_A498_T0.6610.52
372_T376_T0.6610.52
475_V530_K0.6600.52
513_A516_D0.6580.52
135_Q138_D0.6580.52
497_V510_A0.6580.52
195_T198_P0.6580.52
231_V295_P0.6580.52
463_A486_E0.6570.51
437_D442_R0.6570.51
231_V234_D0.6550.51
467_A485_S0.6540.51
353_D422_D0.6530.51
513_A517_L0.6520.51
379_S493_L0.6510.51
341_A424_L0.6500.51
278_I282_G0.6500.51
240_V244_N0.6480.50
57_T73_F0.6470.50
250_A259_N0.6470.50
144_S149_K0.6460.50
152_H156_R0.6460.50
363_S412_E0.6440.50
53_V57_T0.6440.50
369_H373_H0.6410.50
60_E64_S0.6400.50
368_A464_Y0.6400.50
489_Y510_A0.6370.49
92_P95_K0.6370.49
320_F378_Y0.6330.49
407_D468_E0.6330.49
519_G524_Q0.6330.49
408_W468_E0.6330.49
401_A412_E0.6320.49
495_Y513_A0.6300.49
294_E298_V0.6290.48
431_S437_D0.6290.48
143_P210_K0.6290.48
463_A466_L0.6290.48
153_T207_I0.6280.48
72_Q410_I0.6260.48
477_V527_S0.6250.48
241_T251_D0.6250.48
442_R503_F0.6250.48
313_K487_Q0.6230.48
487_Q494_T0.6230.48
488_I510_A0.6220.48
369_H397_D0.6210.48
304_A371_I0.6210.48
240_V249_L0.6200.47
228_G233_G0.6190.47
319_S324_G0.6190.47
394_S460_N0.6180.47
148_K151_I0.6160.47
203_W217_S0.6160.47
353_D414_V0.6150.47
483_E487_Q0.6140.47
337_W360_L0.6130.47
55_N58_Q0.6130.47
230_G240_V0.6130.47
441_N458_I0.6100.46
70_Q75_L0.6090.46
511_I528_V0.6090.46
527_S531_A0.6080.46
311_Y490_Y0.6080.46
200_P337_W0.6070.46
312_S432_K0.6060.46
75_L83_F0.6050.46
58_Q99_F0.6050.46
241_T244_N0.6010.45
440_S446_T0.6010.45
341_A360_L0.6000.45
105_Y136_I0.6000.45
55_N64_S0.5990.45
511_I533_E0.5990.45
190_L206_F0.5990.45
304_A361_S0.5980.45
260_T263_A0.5980.45
50_Q60_E0.5970.45
332_H350_G0.5970.45
357_F361_S0.5960.45
242_E247_F0.5950.45
150_A212_G0.5940.45
370_E393_E0.5940.45
401_A406_D0.5940.45
138_D182_D0.5930.45
325_A378_Y0.5930.45
154_A191_V0.5920.44
475_V533_E0.5920.44
373_H393_E0.5910.44
300_A360_L0.5910.44
170_G193_L0.5910.44
239_D242_E0.5900.44
410_I482_R0.5890.44
259_N266_L0.5890.44
83_F519_G0.5880.44
371_I374_A0.5870.44
501_T513_A0.5870.44
97_T223_E0.5850.44
75_L132_V0.5840.44
430_P461_K0.5830.43
491_R496_Y0.5830.43
357_F366_I0.5830.43
376_T460_N0.5820.43
232_S236_K0.5820.43
132_V138_D0.5800.43
398_I486_E0.5800.43
430_P457_S0.5790.43
466_L527_S0.5790.43
309_D511_I0.5790.43
265_N293_N0.5770.43
91_D118_A0.5760.43
241_T246_R0.5760.43
83_F124_Q0.5760.43
465_L468_E0.5760.43
226_G280_Y0.5750.43
98_F129_F0.5740.42
62_L85_V0.5740.42
382_L396_S0.5740.42
122_N208_D0.5740.42
309_D323_N0.5740.42
263_A348_V0.5740.42
395_I398_I0.5710.42
459_H506_M0.5710.42
70_Q74_K0.5690.42
353_D424_L0.5680.42
408_W430_P0.5670.42
53_V64_S0.5670.42
62_L117_V0.5640.41
310_Y483_E0.5640.41
300_A304_A0.5640.41
211_N375_V0.5630.41
428_E464_Y0.5630.41
239_D257_G0.5620.41
331_V361_S0.5570.41
105_Y185_Y0.5570.41
307_V403_A0.5550.40
338_N414_V0.5550.40
267_N336_Q0.5550.40
387_E459_H0.5540.40
488_I528_V0.5530.40
244_N248_Y0.5530.40
394_S456_S0.5520.40
83_F103_E0.5520.40
176_L179_Y0.5520.40
261_F277_L0.5520.40
395_I506_M0.5520.40
102_T114_E0.5510.40
177_Y206_F0.5510.40
260_T304_A0.5500.40
386_G454_I0.5500.40
258_I327_I0.5500.40
319_S323_N0.5470.40
57_T181_H0.5460.40
338_N417_P0.5450.39
333_V351_D0.5440.39
396_S493_L0.5430.39
443_Y447_E0.5430.39
388_P460_N0.5430.39
329_S347_M0.5420.39
318_D325_A0.5420.39
485_S532_Y0.5420.39
308_Y325_A0.5390.39
404_D412_E0.5380.39
238_F244_N0.5380.39
370_E397_D0.5380.39
384_Y396_S0.5370.39
240_V285_I0.5370.39
285_I292_F0.5360.38
373_H397_D0.5360.38
62_L99_F0.5350.38
111_Y190_L0.5340.38
88_T99_F0.5330.38
430_P463_A0.5330.38
335_K341_A0.5330.38
157_E372_T0.5320.38
384_Y455_N0.5320.38
229_I232_S0.5310.38
312_S316_G0.5310.38
358_K416_T0.5300.38
292_F295_P0.5280.38
144_S168_L0.5280.38
317_R379_S0.5270.38
368_A404_D0.5270.38
408_W464_Y0.5260.37
320_F396_S0.5260.37
116_A129_F0.5260.37
269_T272_T0.5250.37
244_N247_F0.5250.37
143_P161_S0.5250.37
132_V136_I0.5250.37
462_A489_Y0.5240.37
138_D141_V0.5240.37
108_I183_G0.5240.37
67_D72_Q0.5230.37
442_R446_T0.5220.37
353_D357_F0.5220.37
103_E374_A0.5210.37
261_F330_T0.5210.37
435_N454_I0.5210.37
501_T506_M0.5200.37
278_I281_T0.5190.37
151_I174_G0.5190.37
465_L469_G0.5180.37
457_S461_K0.5170.37
281_T284_E0.5170.37
387_E504_S0.5170.37
306_A364_L0.5160.36
260_T329_S0.5150.36
228_G238_F0.5150.36
146_S156_R0.5150.36
177_Y302_A0.5140.36
426_S454_I0.5140.36
308_Y317_R0.5140.36
279_G283_K0.5140.36
443_Y454_I0.5130.36
259_N326_R0.5130.36
361_S367_V0.5130.36
514_A520_E0.5130.36
255_G309_D0.5120.36
70_Q132_V0.5110.36
54_Q61_S0.5110.36
483_E518_Y0.5110.36
386_G455_N0.5100.36
208_D212_G0.5090.36
439_Y536_G0.5090.36
353_D415_Y0.5090.36
461_K535_V0.5080.36
426_S455_N0.5080.36
309_D312_S0.5080.36
140_S187_L0.5070.36
194_S202_Y0.5070.36
86_S152_H0.5070.36
83_F158_L0.5060.35
490_Y494_T0.5060.35
363_S376_T0.5050.35
457_S535_V0.5050.35
101_L104_V0.5050.35
340_A425_R0.5040.35
382_L497_V0.5030.35
226_G241_T0.5020.35
238_F247_F0.5020.35
231_V361_S0.5020.35
286_V289_T0.5020.35
59_K63_F0.5020.35
497_V505_M0.5000.35
79_A84_R0.5000.35
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4gerA 1 0.5651 100 0.396 Contact Map
4n4eE 1 0.5781 100 0.404 Contact Map
1bqbA 1 0.5502 100 0.423 Contact Map
3nqxA 1 0.5242 100 0.483 Contact Map
2vqxA 1 0.5632 100 0.488 Contact Map
4k89A 1 0.5279 100 0.497 Contact Map
4k90A 1 0.5929 100 0.508 Contact Map
3nqzA 1 0.3197 100 0.788 Contact Map
4k90B 1 0.3253 99.9 0.809 Contact Map
2o3oA 2 0.368 98.4 0.921 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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