GREMLIN Database
PCF - Positive control factor
UniProt: P39784 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 169 (151)
Sequences: 125 (103)
Seq/√Len: 8.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
51_V55_L4.2360.98
144_S148_T3.5070.95
125_H135_I3.4460.94
132_Y143_K2.9580.88
131_S143_K2.8150.85
5_L52_T2.7370.84
126_K154_S2.7020.83
71_D76_L2.5890.80
51_V145_T2.5130.78
67_I70_R2.5090.78
120_E138_L2.3690.73
71_D74_Q2.3590.73
117_R139_L2.1530.66
19_Y55_L2.0910.63
158_Q161_Q1.9620.58
124_L130_F1.8530.54
44_M47_D1.8390.53
74_Q107_I1.7770.50
115_T121_M1.7690.50
115_T148_T1.7390.49
18_Q41_I1.7020.47
12_L49_E1.6860.47
52_T131_S1.6840.46
11_T15_T1.6680.46
142_K145_T1.6530.45
37_D60_Q1.6500.45
64_R69_R1.6380.45
129_C141_V1.6290.44
104_R116_D1.6110.43
148_T151_K1.5370.40
11_T157_M1.5210.40
19_Y150_I1.5060.39
102_E109_E1.4650.38
11_T48_L1.4650.38
50_Y54_W1.4500.37
118_E156_K1.4410.37
40_I124_L1.3840.34
111_L120_E1.3590.33
68_D85_E1.3530.33
131_S147_Q1.3290.32
128_E139_L1.3060.31
4_L8_Y1.3050.31
82_R85_E1.2970.31
115_T149_T1.2600.30
15_T48_L1.2280.29
119_K127_V1.1910.27
111_L114_L1.1840.27
63_I142_K1.1820.27
141_V146_V1.1740.27
136_A161_Q1.1730.27
147_Q153_A1.1660.27
153_A156_K1.1610.26
84_I87_F1.1500.26
146_V153_A1.1230.25
18_Q161_Q1.1210.25
7_E99_V1.1140.25
63_I101_E1.1140.25
55_L59_R1.1110.25
121_M124_L1.1100.25
100_S142_K1.0890.24
65_R70_R1.0840.24
13_K117_R1.0800.24
3_D6_F1.0780.24
67_I120_E1.0740.24
13_K41_I1.0730.24
6_F119_K1.0660.24
42_R47_D1.0650.23
12_L39_K1.0600.23
125_H147_Q1.0480.23
59_R73_Y1.0350.23
54_W73_Y1.0320.22
9_K49_E1.0310.22
69_R110_A1.0160.22
16_R19_Y1.0110.22
81_P110_A1.0070.22
103_D106_R0.9990.22
132_Y150_I0.9820.21
84_I108_R0.9770.21
71_D75_R0.9730.21
65_R71_D0.9680.21
144_S151_K0.9670.21
91_M134_R0.9620.21
69_R81_P0.9540.20
16_R37_D0.9510.20
41_I123_L0.9380.20
13_K55_L0.9290.20
72_V143_K0.9250.20
70_R74_Q0.9180.19
82_R87_F0.9170.19
70_R75_R0.9170.19
85_E120_E0.9120.19
9_K77_M0.9060.19
83_I107_I0.8950.19
95_P98_Q0.8950.19
42_R103_D0.8930.19
96_D150_I0.8920.19
123_L142_K0.8870.19
132_Y147_Q0.8760.18
107_I113_L0.8690.18
68_D125_H0.8610.18
42_R63_I0.8600.18
113_L160_Q0.8590.18
141_V145_T0.8580.18
97_G141_V0.8550.18
72_V76_L0.8530.18
113_L159_R0.8450.17
55_L93_F0.8440.17
62_G69_R0.8390.17
34_E50_Y0.8360.17
2_Q101_E0.8270.17
119_K157_M0.8270.17
4_L7_E0.8260.17
53_E62_G0.8220.17
134_R157_M0.8200.17
14_Q144_S0.8120.17
67_I74_Q0.8080.17
7_E90_A0.8070.17
59_R68_D0.8060.17
47_D73_Y0.8040.17
20_K37_D0.7970.16
149_T159_R0.7950.16
9_K48_L0.7900.16
44_M66_A0.7890.16
83_I150_I0.7890.16
58_G121_M0.7830.16
19_Y39_K0.7800.16
45_I49_E0.7790.16
33_E55_L0.7760.16
39_K157_M0.7640.16
9_K56_E0.7620.16
104_R110_A0.7580.15
59_R66_A0.7560.15
68_D73_Y0.7540.15
126_K147_Q0.7510.15
4_L61_P0.7430.15
55_L154_S0.7310.15
150_I153_A0.7300.15
78_I132_Y0.7270.15
54_W59_R0.7250.15
143_K147_Q0.7240.15
37_D40_I0.7120.14
39_K130_F0.7070.14
68_D76_L0.7060.14
51_V144_S0.7050.14
5_L21_P0.7030.14
133_E145_T0.6960.14
12_L121_M0.6960.14
65_R69_R0.6930.14
74_Q138_L0.6930.14
53_E110_A0.6870.14
15_T41_I0.6860.14
55_L157_M0.6860.14
10_R46_T0.6840.14
121_M153_A0.6820.14
66_A85_E0.6770.14
147_Q151_K0.6720.14
79_K131_S0.6700.14
52_T113_L0.6550.13
102_E112_A0.6550.13
19_Y111_L0.6540.13
63_I141_V0.6470.13
66_A135_I0.6410.13
13_K43_N0.6380.13
107_I133_E0.6360.13
81_P85_E0.6310.13
115_T138_L0.6270.13
89_S145_T0.6260.13
18_Q37_D0.6240.13
146_V150_I0.6180.13
59_R145_T0.6180.13
100_S110_A0.6160.13
12_L45_I0.6130.13
43_N64_R0.6090.12
86_S138_L0.6080.12
81_P107_I0.6060.12
13_K49_E0.6010.12
20_K129_C0.5990.12
14_Q20_K0.5940.12
58_G134_R0.5930.12
8_Y152_R0.5930.12
52_T56_E0.5850.12
7_E104_R0.5810.12
150_I158_Q0.5810.12
116_D157_M0.5800.12
64_R122_F0.5770.12
33_E43_N0.5760.12
121_M149_T0.5760.12
144_S147_Q0.5750.12
44_M123_L0.5700.12
120_E130_F0.5700.12
76_L84_I0.5690.12
15_T85_E0.5660.12
5_L73_Y0.5630.12
125_H131_S0.5590.12
45_I54_W0.5540.12
12_L16_R0.5530.11
42_R101_E0.5510.11
84_I88_S0.5510.11
18_Q150_I0.5480.11
4_L131_S0.5470.11
86_S90_A0.5450.11
140_G149_T0.5450.11
59_R150_I0.5430.11
101_E158_Q0.5420.11
57_K64_R0.5410.11
48_L87_F0.5390.11
94_E155_L0.5390.11
12_L113_L0.5380.11
87_F114_L0.5370.11
60_Q68_D0.5360.11
121_M139_L0.5320.11
19_Y38_K0.5320.11
118_E153_A0.5300.11
115_T119_K0.5270.11
74_Q85_E0.5240.11
95_P158_Q0.5200.11
17_I99_V0.5170.11
43_N47_D0.5160.11
106_R110_A0.5160.11
65_R143_K0.5150.11
2_Q37_D0.5100.11
114_L120_E0.5080.11
99_V145_T0.5060.11
68_D82_R0.5050.11
62_G68_D0.5030.11
109_E157_M0.5000.11
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1xsvA 2 0.432 98.9 0.805 Contact Map
3hugA 3 0.4734 98.9 0.809 Contact Map
1ku3A 1 0.3373 98.8 0.811 Contact Map
2q1zA 1 0.858 98.8 0.811 Contact Map
2lfwA 1 0.858 98.8 0.813 Contact Map
4cxfA 1 0.787 98.8 0.814 Contact Map
4nqwA 1 0.7633 98.8 0.814 Contact Map
3t72q 1 0.432 98.8 0.814 Contact Map
2rnjA 1 0.355 98.8 0.815 Contact Map
1or7A 2 0.8935 98.8 0.815 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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