GREMLIN Database
ROCA - 1-pyrroline-5-carboxylate dehydrogenase
UniProt: P39634 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 515 (463)
Sequences: 35959 (21761)
Seq/√Len: 1011.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
330_Q428_R5.1151.00
268_E272_K3.8991.00
330_Q426_K3.2081.00
302_D339_K3.1211.00
333_Y426_K2.8551.00
407_D410_A2.7941.00
389_E399_Q2.7941.00
53_I66_Y2.7391.00
83_L250_K2.7361.00
297_K329_H2.7331.00
59_N62_E2.7081.00
365_E369_N2.6791.00
377_I411_R2.5501.00
337_V424_I2.5491.00
306_S343_L2.5241.00
109_R142_E2.4471.00
376_E380_S2.4431.00
216_K219_E2.4361.00
297_K331_D2.4131.00
111_R114_E2.3591.00
375_I398_I2.3321.00
83_L252_R2.2811.00
53_I63_I2.2461.00
450_T456_I2.2341.00
71_D74_L2.2071.00
42_N84_Q2.2041.00
104_A221_M2.1291.00
39_L70_A2.1081.00
222_E228_N2.0981.00
372_M376_E2.0881.00
383_K405_D2.0721.00
112_K135_A2.0641.00
377_I381_E2.0621.00
430_F455_H2.0411.00
107_I223_E2.0241.00
362_V371_V2.0211.00
372_M390_G2.0201.00
170_E509_K1.9691.00
341_V345_K1.9541.00
243_D247_E1.9491.00
209_A364_H1.9451.00
441_E489_M1.9371.00
247_E269_R1.9111.00
100_I226_L1.8911.00
372_M398_I1.8881.00
458_R462_D1.8681.00
293_V308_I1.8631.00
335_E339_K1.8461.00
114_E216_K1.8321.00
40_V222_E1.8311.00
46_I77_K1.8281.00
385_L404_A1.8041.00
403_F412_L1.8001.00
113_H131_D1.7941.00
107_I224_A1.7881.00
298_D455_H1.7871.00
430_F458_R1.7761.00
379_K384_L1.7661.00
169_Y503_I1.7601.00
265_R268_E1.7561.00
80_Q250_K1.7521.00
169_Y506_M1.7431.00
464_H488_N1.7411.00
327_V436_I1.7401.00
76_E244_F1.7361.00
327_V433_M1.7341.00
264_C489_M1.7241.00
107_I110_R1.7141.00
111_R223_E1.6881.00
107_I220_V1.6781.00
76_E80_Q1.6771.00
408_E436_I1.6761.00
329_H427_A1.6751.00
344_T402_I1.6651.00
413_M425_C1.6591.00
341_V385_L1.6351.00
383_K404_A1.6251.00
308_I336_V1.6201.00
432_H435_E1.6061.00
102_F142_E1.5991.00
118_Y216_K1.5981.00
86_F90_K1.5841.00
218_V233_Y1.5831.00
411_R415_E1.5751.00
338_E342_A1.5621.00
333_Y337_V1.5601.00
326_A422_V1.5541.00
301_L339_K1.5441.00
201_T230_V1.5431.00
141_L193_V1.5291.00
106_A110_R1.5281.00
63_I66_Y1.5231.00
313_F344_T1.5221.00
237_D240_E1.5211.00
373_K411_R1.5191.00
76_E248_H1.5121.00
384_L403_F1.5121.00
334_D426_K1.5061.00
109_R138_I1.5031.00
93_R96_H1.5001.00
428_R432_H1.4881.00
431_D462_D1.4751.00
106_A142_E1.4611.00
365_E396_Y1.4541.00
339_K342_A1.4491.00
102_F149_L1.4481.00
297_K455_H1.4471.00
103_K106_A1.4291.00
96_H99_N1.4271.00
305_A339_K1.4261.00
376_E379_K1.4211.00
446_G465_V1.4051.00
296_D455_H1.4001.00
219_E223_E1.3981.00
44_E228_N1.3971.00
62_E394_K1.3961.00
307_S449_L1.3881.00
335_E338_E1.3861.00
343_L346_T1.3851.00
113_H117_S1.3821.00
333_Y404_A1.3751.00
327_V427_A1.3731.00
74_L77_K1.3731.00
308_I326_A1.3691.00
105_A142_E1.3661.00
91_K462_D1.3631.00
51_K66_Y1.3631.00
147_Q150_K1.3561.00
117_S121_K1.3561.00
381_E411_R1.3501.00
328_I336_V1.3481.00
60_K394_K1.3461.00
177_V202_I1.3431.00
120_V130_A1.3351.00
141_L189_A1.3341.00
374_Y417_I1.3291.00
168_H461_E1.3231.00
371_V419_G1.3111.00
432_H436_I1.3061.00
267_Y492_T1.3051.00
92_Q96_H1.3021.00
296_D427_A1.3021.00
86_F201_T1.2981.00
348_T356_D1.2941.00
117_S353_E1.2881.00
218_V231_L1.2751.00
342_A345_K1.2701.00
368_Y398_I1.2691.00
56_N59_N1.2681.00
434_L459_A1.2671.00
111_R216_K1.2661.00
55_I63_I1.2641.00
253_F284_I1.2601.00
369_N373_K1.2541.00
427_A436_I1.2531.00
50_R69_T1.2521.00
188_M204_L1.2511.00
345_K386_A1.2511.00
368_Y372_M1.2411.00
40_V45_K1.2401.00
296_D459_A1.2381.00
429_D432_H1.2341.00
334_D338_E1.2321.00
221_M231_L1.2211.00
105_A109_R1.2151.00
127_W131_D1.2121.00
115_F216_K1.2111.00
368_Y390_G1.2071.00
79_M248_H1.2001.00
365_E368_Y1.1981.00
337_V341_V1.1971.00
329_H429_D1.1861.00
247_E272_K1.1831.00
329_H332_V1.1751.00
243_D269_R1.1671.00
80_Q84_Q1.1631.00
294_L433_M1.1611.00
169_Y508_A1.1581.00
351_N354_D1.1571.00
381_E410_A1.1571.00
166_Q457_E1.1551.00
500_D503_I1.1541.00
350_G357_T1.1431.00
348_T358_Y1.1421.00
69_T237_D1.1411.00
75_A241_I1.1401.00
104_A220_V1.1381.00
414_Q439_N1.1361.00
409_N414_Q1.1351.00
378_G403_F1.1341.00
340_A422_V1.1321.00
499_P503_I1.1311.00
446_G468_L1.1311.00
332_V335_E1.1301.00
117_S127_W1.1271.00
243_D265_R1.1261.00
120_V352_P1.1241.00
408_E439_N1.1241.00
192_A231_L1.1231.00
347_L359_M1.1231.00
373_K377_I1.1221.00
92_Q100_I1.1201.00
336_V340_A1.1191.00
103_K107_I1.1161.00
208_D237_D1.1141.00
79_M250_K1.1111.00
104_A226_L1.1111.00
208_D239_A1.1081.00
104_A141_L1.1071.00
460_R511_T1.1061.00
414_Q441_E1.1041.00
56_N351_N1.1041.00
262_V265_R1.1031.00
342_A346_T1.1031.00
337_V404_A1.0981.00
244_F248_H1.0861.00
431_D458_R1.0841.00
456_I470_F1.0791.00
294_L459_A1.0771.00
339_K343_L1.0731.00
337_V385_L1.0721.00
177_V255_S1.0691.00
500_D508_A1.0681.00
58_A352_P1.0661.00
79_M251_T1.0641.00
391_D399_Q1.0621.00
265_R269_R1.0601.00
65_G212_V1.0601.00
434_L462_D1.0561.00
155_A499_P1.0541.00
300_D451_K1.0461.00
341_V386_A1.0461.00
112_K131_D1.0441.00
267_Y283_V1.0441.00
72_Q244_F1.0441.00
345_K399_Q1.0441.00
111_R220_V1.0431.00
102_F146_R1.0411.00
110_R223_E1.0371.00
431_D435_E1.0371.00
52_I69_T1.0341.00
215_A235_P1.0331.00
115_F220_V1.0301.00
430_F459_A1.0291.00
293_V323_G1.0261.00
294_L437_A1.0251.00
172_L253_F1.0231.00
60_K396_Y1.0211.00
379_K403_F1.0201.00
261_A265_R1.0191.00
192_A202_I1.0161.00
85_A201_T1.0141.00
176_I251_T1.0111.00
87_D435_E1.0091.00
452_N455_H1.0091.00
69_T240_E1.0081.00
371_V421_V1.0081.00
176_I245_L1.0071.00
264_C280_L1.0021.00
183_F319_K1.0001.00
229_G232_N1.0001.00
137_A189_A0.9911.00
215_A233_Y0.9901.00
368_Y396_Y0.9891.00
387_G399_Q0.9831.00
387_G401_T0.9801.00
44_E77_K0.9791.00
290_K324_S0.9791.00
406_V410_A0.9761.00
300_D303_L0.9721.00
377_I380_S0.9671.00
292_T446_G0.9641.00
81_A232_N0.9571.00
40_V218_V0.9571.00
494_S507_Q0.9571.00
87_D464_H0.9561.00
42_N81_A0.9521.00
168_H511_T0.9521.00
208_D238_G0.9491.00
87_D91_K0.9471.00
267_Y271_A0.9461.00
374_Y377_I0.9371.00
98_A149_L0.9341.00
56_N121_K0.9331.00
427_A433_M0.9331.00
334_D337_V0.9281.00
154_G157_V0.9261.00
174_V251_T0.9261.00
148_M197_V0.9251.00
485_G494_S0.9251.00
252_R464_H0.9211.00
447_A473_G0.9191.00
174_V201_T0.9171.00
72_Q240_E0.9171.00
48_T71_D0.9151.00
111_R219_E0.9151.00
170_E461_E0.9121.00
487_F507_Q0.9111.00
302_D335_E0.9091.00
219_E222_E0.9061.00
172_L282_R0.9031.00
90_K464_H0.9021.00
259_S262_V0.9011.00
311_S323_G0.8981.00
121_K353_E0.8981.00
114_E117_S0.8971.00
83_L87_D0.8921.00
374_Y411_R0.8871.00
312_A324_S0.8861.00
296_D430_F0.8861.00
165_N482_Q0.8861.00
56_N62_E0.8811.00
433_M436_I0.8771.00
408_E425_C0.8771.00
105_A145_A0.8771.00
309_V343_L0.8741.00
305_A340_A0.8741.00
108_L137_A0.8741.00
371_V417_I0.8741.00
435_E439_N0.8731.00
39_L235_P0.8731.00
75_A176_I0.8721.00
125_K133_D0.8711.00
102_F145_A0.8701.00
98_A145_A0.8701.00
333_Y424_I0.8671.00
448_L459_A0.8671.00
72_Q76_E0.8671.00
123_A210_A0.8631.00
425_C436_I0.8631.00
373_K376_E0.8591.00
175_G253_F0.8591.00
94_P98_A0.8571.00
301_L305_A0.8561.00
305_A343_L0.8531.00
457_E461_E0.8531.00
378_G417_I0.8511.00
295_V336_V0.8481.00
388_G401_T0.8481.00
98_A102_F0.8471.00
282_R507_Q0.8461.00
85_A230_V0.8461.00
99_N103_K0.8451.00
427_A432_H0.8451.00
137_A185_L0.8441.00
170_E487_F0.8441.00
446_G463_F0.8431.00
303_L307_S0.8411.00
107_I111_R0.8401.00
254_V266_I0.8391.00
293_V449_L0.8381.00
369_N372_M0.8361.00
304_A308_I0.8341.00
153_E157_V0.8331.00
55_I396_Y0.8311.00
305_A336_V0.8291.00
296_D450_T0.8241.00
172_L200_N0.8211.00
172_L507_Q0.8181.00
267_Y270_A0.8141.00
209_A366_A0.8141.00
434_L438_N0.8131.00
204_L214_A0.8111.00
264_C268_E0.8101.00
375_I421_V0.8101.00
482_Q510_T0.8101.00
267_Y280_L0.8081.00
91_K461_E0.8061.00
73_E76_E0.8021.00
52_I237_D0.8001.00
305_A309_V0.7981.00
226_L231_L0.7921.00
77_K81_A0.7921.00
42_N228_N0.7921.00
108_L138_I0.7921.00
382_G410_A0.7881.00
188_M192_A0.7871.00
254_V270_A0.7861.00
453_R456_I0.7851.00
333_Y405_D0.7851.00
84_Q87_D0.7851.00
101_L197_V0.7831.00
349_V391_D0.7821.00
58_A397_F0.7791.00
73_E77_K0.7771.00
39_L68_S0.7771.00
304_A336_V0.7751.00
371_V398_I0.7751.00
116_S131_D0.7731.00
239_A243_D0.7661.00
123_A363_I0.7641.00
100_I224_A0.7621.00
120_V127_W0.7611.00
403_F406_V0.7591.00
103_K224_A0.7591.00
194_A501_Y0.7581.00
189_A217_F0.7571.00
263_G287_M0.7551.00
198_T506_M0.7551.00
46_I74_L0.7551.00
102_F106_A0.7541.00
82_A232_N0.7541.00
347_L358_Y0.7521.00
309_V340_A0.7521.00
292_T437_A0.7511.00
128_K358_Y0.7501.00
338_E341_V0.7491.00
77_K80_Q0.7491.00
81_A229_G0.7481.00
379_K389_E0.7451.00
176_I254_V0.7421.00
236_G241_I0.7401.00
89_W230_V0.7391.00
215_A219_E0.7381.00
116_S127_W0.7351.00
45_K222_E0.7351.00
206_P235_P0.7341.00
177_V191_T0.7341.00
404_A426_K0.7341.00
291_D324_S0.7331.00
465_V468_L0.7321.00
116_S120_V0.7311.00
95_E149_L0.7311.00
87_D90_K0.7291.00
329_H430_F0.7291.00
263_G285_A0.7281.00
70_A74_L0.7281.00
191_T495_K0.7271.00
256_F262_V0.7271.00
246_V269_R0.7261.00
340_A402_I0.7241.00
374_Y415_E0.7231.00
296_D329_H0.7221.00
246_V266_I0.7221.00
218_V222_E0.7191.00
195_A202_I0.7191.00
127_W353_E0.7171.00
200_N506_M0.7171.00
84_Q88_S0.7151.00
226_L230_V0.7101.00
88_S92_Q0.7101.00
60_K365_E0.7091.00
64_I121_K0.7081.00
429_D435_E0.7061.00
165_N510_T0.7041.00
302_D343_L0.7031.00
56_N353_E0.7021.00
366_A369_N0.7021.00
109_R135_A0.7021.00
75_A79_M0.7011.00
242_G246_V0.7001.00
375_I403_F0.7001.00
354_D357_T0.6991.00
115_F185_L0.6981.00
270_A283_V0.6951.00
154_G158_K0.6951.00
109_R112_K0.6941.00
341_V344_T0.6931.00
185_L213_V0.6921.00
328_I332_V0.6921.00
260_R489_M0.6911.00
55_I60_K0.6911.00
301_L332_V0.6911.00
131_D135_A0.6901.00
159_S165_N0.6891.00
188_M214_A0.6881.00
76_E250_K0.6871.00
386_A402_I0.6861.00
344_T386_A0.6861.00
327_V408_E0.6851.00
368_Y391_D0.6841.00
264_C272_K0.6831.00
365_E390_G0.6821.00
176_I242_G0.6811.00
70_A78_A0.6801.00
351_N357_T0.6781.00
89_W100_I0.6761.00
204_L231_L0.6751.00
460_R470_F0.6751.00
209_A363_I0.6751.00
94_P148_M0.6731.00
398_I401_T0.6721.00
172_L252_R0.6711.00
246_V254_V0.6701.00
451_K454_A0.6681.00
119_L122_E0.6671.00
344_T347_L0.6671.00
240_E243_D0.6661.00
118_Y212_V0.6651.00
381_E409_N0.6651.00
408_E413_M0.6641.00
268_E271_A0.6631.00
90_K462_D0.6621.00
50_R240_E0.6611.00
406_V413_M0.6611.00
121_K212_V0.6611.00
125_K129_E0.6611.00
75_A244_F0.6601.00
349_V359_M0.6591.00
238_G242_G0.6591.00
430_F434_L0.6581.00
282_R504_L0.6581.00
388_G399_Q0.6571.00
364_H367_S0.6561.00
113_H127_W0.6551.00
327_V413_M0.6541.00
203_L232_N0.6531.00
326_A336_V0.6531.00
106_A109_R0.6501.00
298_D450_T0.6491.00
83_L86_F0.6491.00
316_S359_M0.6471.00
221_M226_L0.6471.00
90_K170_E0.6461.00
370_K373_K0.6421.00
308_I340_A0.6421.00
42_N232_N0.6411.00
367_S370_K0.6411.00
372_M375_I0.6391.00
164_V457_E0.6391.00
464_H487_F0.6391.00
436_I439_N0.6381.00
42_N77_K0.6381.00
208_D370_K0.6371.00
295_V304_A0.6371.00
309_V347_L0.6361.00
384_L401_T0.6341.00
453_R457_E0.6331.00
340_A424_I0.6321.00
450_T455_H0.6311.00
90_K171_A0.6301.00
70_A234_I0.6281.00
267_Y285_A0.6271.00
201_T232_N0.6261.00
366_A370_K0.6261.00
114_E219_E0.6251.00
80_Q83_L0.6251.00
241_I244_F0.6231.00
88_S91_K0.6211.00
188_M217_F0.6211.00
67_A211_P0.6201.00
68_S235_P0.6191.00
456_I460_R0.6181.00
82_A201_T0.6181.00
108_L217_F0.6161.00
362_V367_S0.6141.00
284_I504_L0.6131.00
179_S188_M0.6111.00
134_T138_I0.6101.00
372_M379_K0.6101.00
41_I78_A0.6081.00
503_I508_A0.6081.00
503_I506_M0.6071.00
269_R272_K0.6061.00
105_A138_I0.6051.00
87_D462_D0.6041.00
301_L335_E0.6041.00
185_L217_F0.6031.00
155_A500_D0.6031.00
168_H509_K0.6021.00
406_V412_L0.6021.00
151_L506_M0.6011.00
125_K183_F0.6011.00
236_G242_G0.6011.00
45_K219_E0.5991.00
108_L141_L0.5991.00
417_I421_V0.5971.00
85_A88_S0.5961.00
54_S65_G0.5961.00
119_L213_V0.5961.00
293_V326_A0.5961.00
94_P197_V0.5951.00
192_A204_L0.5941.00
165_N512_S0.5931.00
43_G222_E0.5931.00
341_V402_I0.5931.00
208_D265_R0.5921.00
216_K220_V0.5921.00
54_S122_E0.5911.00
131_D355_P0.5901.00
390_G399_Q0.5881.00
303_L306_S0.5881.00
117_S120_V0.5851.00
239_A265_R0.5851.00
303_L451_K0.5841.00
175_G200_N0.5841.00
405_D426_K0.5841.00
328_I424_I0.5841.00
119_L130_A0.5841.00
51_K68_S0.5831.00
120_V353_E0.5821.00
448_L456_I0.5821.00
108_L185_L0.5791.00
54_S211_P0.5751.00
89_W196_I0.5751.00
298_D451_K0.5751.00
434_L458_R0.5741.00
191_T255_S0.5741.00
119_L133_D0.5731.00
96_H100_I0.5731.00
72_Q247_E0.5731.00
100_I104_A0.5731.00
186_A190_G0.5721.00
112_K116_S0.5721.00
246_V279_W0.5721.00
330_Q333_Y0.5711.00
350_G354_D0.5701.00
349_V358_Y0.5681.00
166_Q511_T0.5671.00
362_V398_I0.5671.00
298_D452_N0.5651.00
78_A234_I0.5631.00
154_G500_D0.5621.00
151_L502_L0.5621.00
434_L463_F0.5621.00
134_T185_L0.5601.00
104_A107_I0.5601.00
454_A458_R0.5591.00
454_A457_E0.5591.00
64_I212_V0.5591.00
413_M436_I0.5571.00
105_A141_L0.5571.00
243_D246_V0.5571.00
299_A304_A0.5571.00
253_F504_L0.5561.00
381_E407_D0.5561.00
311_S473_G0.5551.00
375_I401_T0.5541.00
198_T502_L0.5541.00
177_V192_A0.5491.00
319_K322_A0.5481.00
146_R149_L0.5471.00
326_A424_I0.5451.00
272_K279_W0.5451.00
261_A366_A0.5441.00
40_V43_G0.5441.00
115_F134_T0.5431.00
133_D183_F0.5431.00
176_I203_L0.5421.00
312_A422_V0.5401.00
50_R71_D0.5401.00
297_K427_A0.5401.00
463_F468_L0.5391.00
48_T74_L0.5391.00
468_L509_K0.5381.00
293_V447_A0.5381.00
217_F221_M0.5381.00
108_L115_F0.5381.00
143_F147_Q0.5371.00
175_G202_I0.5361.00
260_R264_C0.5351.00
464_H507_Q0.5341.00
39_L74_L0.5331.00
291_D445_T0.5301.00
286_E444_L0.5291.00
292_T465_V0.5291.00
313_F347_L0.5281.00
81_A228_N0.5261.00
244_F247_E0.5251.00
301_L304_A0.5251.00
144_Y194_A0.5241.00
83_L174_V0.5241.00
430_F462_D0.5241.00
112_K138_I0.5241.00
174_V252_R0.5231.00
412_L417_I0.5231.00
41_I46_I0.5231.00
67_A212_V0.5221.00
203_L234_I0.5221.00
408_E435_E0.5221.00
123_A315_Y0.5211.00
384_L387_G0.5191.00
323_G447_A0.5191.00
81_A85_A0.5181.00
136_E186_A0.5141.00
469_Y472_R0.5141.00
72_Q243_D0.5141.00
135_A138_I0.5111.00
116_S134_T0.5101.00
408_E414_Q0.5091.00
82_A203_L0.5091.00
82_A174_V0.5091.00
305_A332_V0.5091.00
153_E158_K0.5071.00
154_G159_S0.5061.00
379_K382_G0.5061.00
338_E345_K0.5061.00
459_A463_F0.5051.00
328_I333_Y0.5051.00
170_E464_H0.5051.00
389_E392_D0.5041.00
332_V427_A0.5031.00
116_S130_A0.5011.00
448_L470_F0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4nm9A 2 0.9981 100 0.068 Contact Map
1uzbA 5 0.9981 100 0.075 Contact Map
3qanA 2 0.9961 100 0.077 Contact Map
4jdcA 5 0.9786 100 0.092 Contact Map
4oe6A 5 0.9029 100 0.096 Contact Map
4pz2A 4 0.9165 100 0.097 Contact Map
4o5hA 4 0.967 100 0.097 Contact Map
4pxlA 4 0.9204 100 0.098 Contact Map
4yweA 5 0.9068 100 0.099 Contact Map
4lihA 5 0.9398 100 0.101 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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