DHE2 - BACSU - GREMLIN Contacts

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DHE2 - Catabolic NAD-specific glutamate dehydrogenase RocG

UniProt:	P39633	Sequences: MSAKQVSKDEEKEALNLFLSTQTIIKEALRKLGYPGDMYELMKEPQRMLTVRIPVKMDNGSVKVFTGYRSQHNDAVGPTKGGVRFHPEVNEEEVKALSIWMTLKCGIANLPYGGGKGGIICDPRTMSFGELERLSRGYVRAISQIVGPTKDIPAPDVYTNSQIMAWMMDEYSRLREFDSPGFITGKPLVLGGSQGRETATAQGVTICIEEAVKKKGIKLQNARIIIQGFGNAGSFLAKFMHDAGAKVIGISDANGGLYNPDGLDIPYLLDKRDSFGMVTNLFTDVITNEELLEKDCDILVPAAISNQITAKNAHNIQASIVVEAANGPTTIDATKILNERGVLLVPDILASAGGVTVSYFEWVQNNQGYYWSEEEVAEKLRSVMVSSFETIYQTAATHKVDMRLAAYMTGIRKSAEASRFRGWV	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	424 (367)
Sequences:	5506 (2596)
Seq/√Len:	135.5

ECOLI - DHE4
BACSU - DHE2 GUDB DHLE
HALSA - DHEB DHE4 DHE
SULSO - Q97X22 DHE2 Q97Y81 Q97WS2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
240_M	245_A	4.409	1.00
293_E	315_N	3.791	1.00
226_I	237_A	3.613	1.00
296_C	318_A	3.397	1.00
225_I	296_C	3.363	1.00
208_I	322_V	3.161	1.00
270_D	274_S	2.975	1.00
295_D	317_Q	2.950	1.00
335_K	339_E	2.880	1.00
112_Y	359_Y	2.618	1.00
129_G	133_R	2.584	1.00
122_D	125_T	2.518	1.00
249_G	291_L	2.441	1.00
337_L	342_V	2.434	1.00
105_C	355_V	2.431	1.00
223_R	246_K	2.421	1.00
210_E	213_K	2.412	1.00
338_N	401_D	2.353	1.00
157_V	304_I	2.352	1.00
210_E	385_V	2.349	1.00
264_D	267_Y	2.323	1.00
394_T	398_H	2.291	1.00
237_A	250_I	2.283	1.00
238_K	242_D	2.260	1.00
72_H	98_S	2.212	1.00
140_R	170_E	2.176	1.00
57_M	63_K	2.173	1.00
217_I	222_A	2.143	1.00
214_K	396_A	2.142	1.00
204_V	324_A	2.126	1.00
224_I	245_A	2.117	1.00
224_I	240_M	2.112	1.00
207_C	350_A	2.099	1.00
401_D	404_L	2.080	1.00
388_F	392_Y	2.058	1.00
412_R	416_E	2.047	1.00
160_N	163_I	2.046	1.00
223_R	244_G	2.022	1.00
54_P	137_G	2.018	1.00
310_A	332_D	2.015	1.00
322_V	350_A	2.009	1.00
393_Q	397_T	2.002	1.00
329_T	337_L	1.951	1.00
195_G	373_E	1.926	1.00
394_T	412_R	1.925	1.00
292_L	299_L	1.914	1.00
345_V	350_A	1.878	1.00
214_K	392_Y	1.866	1.00
127_S	130_E	1.860	1.00
210_E	389_E	1.856	1.00
250_I	268_L	1.846	1.00
313_A	336_I	1.845	1.00
308_I	329_T	1.841	1.00
206_I	209_E	1.837	1.00
83_V	167_M	1.822	1.00
121_C	126_M	1.800	1.00
372_S	375_E	1.770	1.00
219_L	245_A	1.767	1.00
309_T	312_N	1.761	1.00
348_I	407_Y	1.760	1.00
79_T	115_G	1.749	1.00
205_T	243_A	1.748	1.00
375_E	379_K	1.736	1.00
189_V	416_E	1.735	1.00
70_S	98_S	1.731	1.00
301_P	325_A	1.715	1.00
400_V	408_M	1.708	1.00
249_G	294_K	1.702	1.00
76_V	109_N	1.698	1.00
211_A	320_I	1.683	1.00
287_T	290_E	1.683	1.00
269_L	273_D	1.680	1.00
90_N	93_E	1.670	1.00
253_A	272_R	1.667	1.00
140_R	173_R	1.653	1.00
260_P	284_D	1.640	1.00
308_I	316_I	1.629	1.00
139_V	167_M	1.606	1.00
214_K	388_F	1.605	1.00
58_D	127_S	1.587	1.00
336_I	340_R	1.581	1.00
225_I	249_G	1.575	1.00
145_I	149_T	1.574	1.00
209_E	243_A	1.532	1.00
107_I	349_L	1.529	1.00
218_K	221_N	1.506	1.00
143_S	175_R	1.476	1.00
238_K	269_L	1.476	1.00
212_V	298_I	1.462	1.00
400_V	405_A	1.460	1.00
325_A	328_P	1.459	1.00
165_A	187_P	1.456	1.00
209_E	381_R	1.450	1.00
170_E	173_R	1.440	1.00
211_A	345_V	1.440	1.00
72_H	102_T	1.416	1.00
74_D	79_T	1.392	1.00
224_I	300_V	1.384	1.00
206_I	381_R	1.380	1.00
56_K	129_G	1.372	1.00
331_I	334_T	1.371	1.00
236_L	300_V	1.368	1.00
128_F	132_E	1.363	1.00
204_V	322_V	1.353	1.00
140_R	174_L	1.346	1.00
86_H	304_I	1.336	1.00
349_L	391_I	1.331	1.00
67_G	138_Y	1.328	1.00
127_S	131_L	1.316	1.00
322_V	345_V	1.310	1.00
386_S	390_T	1.289	1.00
121_C	134_L	1.285	1.00
217_I	319_S	1.285	1.00
310_A	336_I	1.282	1.00
377_A	381_R	1.277	1.00
137_G	140_R	1.277	1.00
214_K	389_E	1.275	1.00
232_A	324_A	1.272	1.00
132_E	136_R	1.265	1.00
314_H	336_I	1.260	1.00
267_Y	281_L	1.253	1.00
388_F	402_M	1.248	1.00
206_I	377_A	1.237	1.00
128_F	162_Q	1.234	1.00
53_I	137_G	1.233	1.00
212_V	219_L	1.230	1.00
389_E	393_Q	1.223	1.00
382_S	386_S	1.221	1.00
161_S	197_E	1.214	1.00
215_K	319_S	1.213	1.00
254_N	277_M	1.202	1.00
299_L	316_I	1.199	1.00
103_L	348_I	1.194	1.00
208_I	300_V	1.184	0.99
225_I	294_K	1.170	0.99
136_R	170_E	1.168	0.99
236_L	302_A	1.167	0.99
248_I	296_C	1.166	0.99
365_N	370_Y	1.166	0.99
205_T	240_M	1.159	0.99
373_E	377_A	1.157	0.99
224_I	298_I	1.147	0.99
208_I	298_I	1.147	0.99
103_L	414_S	1.145	0.99
68_Y	120_I	1.144	0.99
102_T	414_S	1.141	0.99
51_V	142_I	1.132	0.99
394_T	409_T	1.131	0.99
271_K	275_F	1.128	0.99
276_G	280_N	1.126	0.99
223_R	297_D	1.121	0.99
256_G	286_I	1.120	0.99
320_I	343_L	1.118	0.99
397_T	412_R	1.118	0.99
223_R	260_P	1.118	0.99
335_K	338_N	1.117	0.99
126_M	134_L	1.105	0.99
70_S	91_E	1.103	0.99
336_I	339_E	1.101	0.99
66_T	120_I	1.082	0.99
215_K	343_L	1.077	0.99
209_E	213_K	1.076	0.99
223_R	295_D	1.074	0.99
224_I	247_V	1.072	0.99
400_V	404_L	1.072	0.99
381_R	385_V	1.070	0.99
225_I	248_I	1.068	0.99
240_M	247_V	1.062	0.99
143_S	174_L	1.061	0.99
271_K	281_L	1.060	0.99
241_H	266_P	1.056	0.99
143_S	147_G	1.054	0.99
234_S	273_D	1.053	0.99
202_Q	239_F	1.046	0.99
54_P	64_V	1.045	0.99
334_T	403_R	1.042	0.99
108_A	356_T	1.040	0.99
248_I	258_Y	1.035	0.99
267_Y	270_D	1.034	0.99
235_F	238_K	1.032	0.99
73_N	418_S	1.026	0.99
204_V	300_V	1.025	0.99
85_F	159_T	1.017	0.98
209_E	219_L	1.015	0.98
85_F	135_S	1.013	0.98
308_I	337_L	1.012	0.98
144_Q	176_E	1.012	0.98
143_S	146_V	1.010	0.98
87_P	122_D	1.009	0.98
137_G	141_A	1.006	0.98
256_G	288_N	1.004	0.98
310_A	314_H	1.003	0.98
265_I	269_L	0.998	0.98
211_A	322_V	0.992	0.98
283_T	286_I	0.991	0.98
204_V	208_I	0.990	0.98
159_T	164_M	0.989	0.98
241_H	265_I	0.980	0.98
396_A	399_K	0.978	0.98
234_S	269_L	0.972	0.98
128_F	131_L	0.968	0.98
137_G	170_E	0.967	0.98
398_H	408_M	0.966	0.98
411_I	414_S	0.965	0.98
51_V	67_G	0.961	0.98
236_L	240_M	0.958	0.98
263_L	268_L	0.952	0.98
204_V	302_A	0.949	0.98
170_E	174_L	0.948	0.98
108_A	352_A	0.946	0.97
127_S	132_E	0.942	0.97
146_V	149_T	0.942	0.97
290_E	293_E	0.941	0.97
246_K	261_D	0.940	0.97
378_E	382_S	0.936	0.97
369_Y	375_E	0.933	0.97
187_P	190_L	0.933	0.97
197_E	235_F	0.931	0.97
259_N	281_L	0.928	0.97
286_I	290_E	0.923	0.97
372_S	382_S	0.922	0.97
135_S	139_V	0.913	0.97
150_K	176_E	0.912	0.97
202_Q	377_A	0.911	0.97
299_L	307_Q	0.911	0.97
356_T	360_F	0.908	0.97
86_H	157_V	0.904	0.97
271_K	280_N	0.902	0.97
394_T	408_M	0.901	0.97
252_D	272_R	0.897	0.97
392_Y	396_A	0.896	0.96
208_I	320_I	0.893	0.96
237_A	247_V	0.892	0.96
311_K	314_H	0.891	0.96
92_E	95_K	0.890	0.96
75_A	414_S	0.888	0.96
88_E	93_E	0.885	0.96
378_E	381_R	0.884	0.96
300_V	322_V	0.882	0.96
126_M	130_E	0.881	0.96
143_S	149_T	0.871	0.96
237_A	268_L	0.870	0.96
226_I	245_A	0.867	0.96
285_V	288_N	0.866	0.96
334_T	338_N	0.865	0.96
83_V	138_Y	0.863	0.96
395_A	400_V	0.861	0.96
393_Q	396_A	0.861	0.96
283_T	288_N	0.859	0.96
316_I	321_V	0.857	0.95
175_R	178_D	0.855	0.95
229_F	250_I	0.853	0.95
308_I	313_A	0.852	0.95
369_Y	372_S	0.848	0.95
204_V	236_L	0.847	0.95
241_H	264_D	0.845	0.95
214_K	393_Q	0.845	0.95
109_N	383_V	0.841	0.95
76_V	106_G	0.840	0.95
368_G	372_S	0.840	0.95
57_M	65_F	0.837	0.95
98_S	102_T	0.835	0.95
358_S	361_E	0.835	0.95
384_M	387_S	0.831	0.95
207_C	391_I	0.830	0.95
240_M	300_V	0.828	0.95
142_I	145_I	0.810	0.94
356_T	384_M	0.808	0.94
385_V	389_E	0.808	0.94
257_L	268_L	0.807	0.94
391_I	394_T	0.807	0.94
83_V	153_P	0.805	0.94
85_F	119_I	0.804	0.94
355_V	358_S	0.800	0.94
116_K	200_T	0.800	0.94
116_K	326_N	0.799	0.93
56_K	63_K	0.797	0.93
321_V	337_L	0.796	0.93
167_M	170_E	0.796	0.93
258_Y	294_K	0.793	0.93
241_H	247_V	0.786	0.93
297_D	319_S	0.786	0.93
135_S	138_Y	0.782	0.93
314_H	340_R	0.778	0.93
267_Y	271_K	0.778	0.93
117_G	138_Y	0.776	0.92
168_M	179_S	0.774	0.92
235_F	239_F	0.770	0.92
126_M	131_L	0.769	0.92
76_V	113_G	0.768	0.92
215_K	320_I	0.767	0.92
329_T	344_L	0.764	0.92
257_L	278_V	0.762	0.92
368_G	375_E	0.753	0.91
219_L	224_I	0.749	0.91
278_V	285_V	0.747	0.91
94_V	98_S	0.740	0.91
376_V	379_K	0.740	0.91
308_I	347_D	0.737	0.90
343_L	402_M	0.737	0.90
258_Y	284_D	0.733	0.90
321_V	329_T	0.733	0.90
238_K	266_P	0.732	0.90
277_M	280_N	0.731	0.90
256_G	293_E	0.731	0.90
68_Y	91_E	0.730	0.90
295_D	318_A	0.728	0.90
124_R	253_A	0.728	0.90
80_K	355_V	0.727	0.90
352_A	356_T	0.722	0.89
200_T	354_G	0.720	0.89
385_V	388_F	0.718	0.89
337_L	344_L	0.717	0.89
390_T	393_Q	0.717	0.89
166_W	169_D	0.715	0.89
310_A	334_T	0.713	0.89
363_V	369_Y	0.712	0.89
310_A	333_A	0.711	0.89
229_F	265_I	0.706	0.88
240_M	298_I	0.706	0.88
266_P	269_L	0.706	0.88
201_A	236_L	0.705	0.88
104_K	326_N	0.705	0.88
397_T	400_V	0.702	0.88
268_L	281_L	0.702	0.88
237_A	265_I	0.699	0.88
398_H	412_R	0.697	0.88
139_V	183_I	0.696	0.88
148_P	179_S	0.695	0.88
237_A	281_L	0.695	0.88
293_E	312_N	0.691	0.87
89_V	118_G	0.690	0.87
106_G	410_G	0.689	0.87
239_F	243_A	0.687	0.87
124_R	272_R	0.686	0.87
63_K	125_T	0.683	0.87
318_A	342_V	0.680	0.87
409_T	413_K	0.678	0.86
149_T	366_N	0.677	0.86
139_V	170_E	0.676	0.86
289_E	293_E	0.674	0.86
329_T	333_A	0.673	0.86
286_I	294_K	0.672	0.86
117_G	135_S	0.669	0.86
202_Q	381_R	0.669	0.86
392_Y	395_A	0.667	0.85
246_K	264_D	0.666	0.85
239_F	377_A	0.665	0.85
379_K	384_M	0.663	0.85
252_D	277_M	0.662	0.85
321_V	333_A	0.662	0.85
55_V	65_F	0.662	0.85
249_G	256_G	0.659	0.85
318_A	321_V	0.659	0.85
155_P	196_R	0.658	0.85
144_Q	148_P	0.656	0.85
344_L	347_D	0.656	0.85
137_G	173_R	0.656	0.85
71_Q	150_K	0.653	0.84
75_A	102_T	0.652	0.84
164_M	167_M	0.652	0.84
212_V	320_I	0.649	0.84
298_I	320_I	0.648	0.84
259_N	283_T	0.648	0.84
57_M	61_S	0.647	0.84
316_I	337_L	0.647	0.84
226_I	250_I	0.647	0.84
58_D	133_R	0.647	0.84
395_A	402_M	0.642	0.83
133_R	136_R	0.642	0.83
168_M	172_S	0.640	0.83
158_Y	232_A	0.639	0.83
193_S	371_W	0.638	0.83
411_I	415_A	0.637	0.83
269_L	272_R	0.637	0.83
379_K	382_S	0.637	0.83
300_V	324_A	0.635	0.83
233_G	252_D	0.635	0.83
100_W	327_G	0.635	0.83
109_N	386_S	0.633	0.82
207_C	322_V	0.632	0.82
268_L	278_V	0.632	0.82
271_K	282_F	0.632	0.82
271_K	276_G	0.630	0.82
259_N	263_L	0.628	0.82
146_V	167_M	0.625	0.82
124_R	127_S	0.624	0.81
232_A	253_A	0.624	0.81
199_A	231_N	0.624	0.81
229_F	268_L	0.622	0.81
359_Y	363_V	0.620	0.81
70_S	115_G	0.618	0.81
248_I	294_K	0.616	0.81
63_K	126_M	0.616	0.81
75_A	418_S	0.615	0.81
308_I	321_V	0.614	0.80
394_T	405_A	0.614	0.80
162_Q	165_A	0.613	0.80
374_E	378_E	0.613	0.80
164_M	190_L	0.612	0.80
209_E	385_V	0.612	0.80
123_P	131_L	0.612	0.80
164_M	183_I	0.609	0.80
309_T	329_T	0.608	0.80
321_V	342_V	0.608	0.80
379_K	383_V	0.607	0.80
188_L	194_Q	0.606	0.80
267_Y	272_R	0.606	0.80
274_S	280_N	0.605	0.79
283_T	287_T	0.604	0.79
270_D	273_D	0.604	0.79
229_F	325_A	0.604	0.79
388_F	391_I	0.603	0.79
167_M	183_I	0.603	0.79
383_V	386_S	0.601	0.79
229_F	301_P	0.601	0.79
53_I	134_L	0.599	0.79
275_F	280_N	0.598	0.79
384_M	388_F	0.597	0.79
393_Q	399_K	0.597	0.79
251_S	292_L	0.596	0.78
148_P	180_P	0.596	0.78
310_A	337_L	0.596	0.78
207_C	345_V	0.594	0.78
292_L	315_N	0.593	0.78
305_S	327_G	0.593	0.78
268_L	272_R	0.592	0.78
309_T	330_T	0.592	0.78
376_V	381_R	0.592	0.78
204_V	350_A	0.592	0.78
357_V	376_V	0.592	0.78
395_A	398_H	0.590	0.78
149_T	368_G	0.590	0.78
324_A	350_A	0.589	0.78
211_A	343_L	0.588	0.78
71_Q	79_T	0.587	0.77
386_S	389_E	0.587	0.77
199_A	380_L	0.585	0.77
196_R	357_V	0.584	0.77
395_A	399_K	0.583	0.77
99_I	103_L	0.583	0.77
334_T	344_L	0.581	0.77
376_V	384_M	0.580	0.77
356_T	383_V	0.580	0.77
52_R	64_V	0.578	0.76
345_V	402_M	0.577	0.76
76_V	413_K	0.576	0.76
403_R	407_Y	0.575	0.76
210_E	214_K	0.575	0.76
198_T	235_F	0.573	0.76
155_P	231_N	0.570	0.75
139_V	142_I	0.570	0.75
308_I	344_L	0.569	0.75
205_T	236_L	0.568	0.75
329_T	334_T	0.567	0.75
135_S	163_I	0.567	0.75
162_Q	166_W	0.563	0.74
364_Q	371_W	0.562	0.74
223_R	248_I	0.561	0.74
392_Y	402_M	0.561	0.74
77_G	147_G	0.561	0.74
266_P	270_D	0.560	0.74
80_K	362_W	0.559	0.74
193_S	357_V	0.559	0.74
284_D	287_T	0.558	0.74
112_Y	362_W	0.558	0.74
121_C	127_S	0.557	0.74
408_M	411_I	0.557	0.74
376_V	387_S	0.557	0.74
107_I	390_T	0.557	0.74
96_A	327_G	0.556	0.74
275_F	278_V	0.555	0.73
314_H	339_E	0.555	0.73
255_G	279_T	0.555	0.73
104_K	116_K	0.555	0.73
225_I	291_L	0.553	0.73
86_H	89_V	0.553	0.73
56_K	62_V	0.553	0.73
123_P	126_M	0.552	0.73
394_T	397_T	0.552	0.73
290_E	312_N	0.551	0.73
205_T	239_F	0.551	0.73
217_I	297_D	0.550	0.73
299_L	321_V	0.548	0.72
313_A	337_L	0.548	0.72
196_R	231_N	0.548	0.72
200_T	231_N	0.548	0.72
227_Q	307_Q	0.547	0.72
226_I	240_M	0.547	0.72
94_V	118_G	0.547	0.72
75_A	417_A	0.547	0.72
225_I	318_A	0.546	0.72
344_L	403_R	0.546	0.72
318_A	337_L	0.546	0.72
207_C	349_L	0.545	0.72
113_G	353_G	0.545	0.72
387_S	391_I	0.545	0.72
238_K	280_N	0.544	0.72
243_A	265_I	0.544	0.72
174_L	183_I	0.543	0.72
254_N	285_V	0.542	0.72
330_T	334_T	0.542	0.72
387_S	390_T	0.540	0.71
223_R	258_Y	0.540	0.71
131_L	135_S	0.538	0.71
199_A	357_V	0.538	0.71
410_G	414_S	0.536	0.71
345_V	388_F	0.536	0.71
122_D	127_S	0.533	0.70
251_S	288_N	0.532	0.70
196_R	358_S	0.531	0.70
255_G	278_V	0.531	0.70
225_I	295_D	0.531	0.70
116_K	154_A	0.530	0.70
394_T	399_K	0.529	0.70
61_S	64_V	0.527	0.70
200_T	351_S	0.526	0.69
135_S	167_M	0.525	0.69
55_V	58_D	0.524	0.69
292_L	307_Q	0.524	0.69
80_K	112_Y	0.524	0.69
95_K	98_S	0.522	0.69
401_D	405_A	0.520	0.69
89_V	93_E	0.520	0.69
65_F	121_C	0.520	0.69
205_T	208_I	0.520	0.69
383_V	387_S	0.518	0.68
405_A	408_M	0.518	0.68
367_Q	375_E	0.515	0.68
213_K	385_V	0.514	0.68
130_E	136_R	0.513	0.67
204_V	232_A	0.513	0.67
308_I	314_H	0.511	0.67
302_A	324_A	0.511	0.67
309_T	315_N	0.511	0.67
136_R	169_D	0.511	0.67
249_G	263_L	0.509	0.67
208_I	324_A	0.508	0.67
202_Q	238_K	0.508	0.67
109_N	390_T	0.508	0.67
252_D	255_G	0.507	0.67
239_F	242_D	0.507	0.67
256_G	294_K	0.505	0.66
371_W	374_E	0.504	0.66
141_A	175_R	0.502	0.66
333_A	338_N	0.502	0.66
211_A	388_F	0.502	0.66
212_V	222_A	0.500	0.66
381_R	384_M	0.500	0.66
176_E	179_S	0.500	0.66

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3r3jA	5	0.9976	100	0.088	Contact Map
4xgiA	6	0.9717	100	0.089	Contact Map
2yfqA	5	0.9222	100	0.089	Contact Map
4fccA	5	0.9976	100	0.09	Contact Map
2bmaA	5	0.9976	100	0.092	Contact Map
1bgvA	5	1	100	0.098	Contact Map
3k92A	5	0.9858	100	0.1	Contact Map
2tmgA	5	0.9575	100	0.101	Contact Map
1v9lA	4	0.967	100	0.103	Contact Map
3aoeE	3	0.9811	100	0.103	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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