GREMLIN Database
SPSF - Spore coat polysaccharide biosynthesis protein SpsF
UniProt: P39626 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 240 (201)
Sequences: 4837 (3792)
Seq/√Len: 267.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
28_N36_H4.1001.00
50_N70_K3.7881.00
6_F48_R2.8121.00
19_G59_E2.7391.00
64_L69_I2.6071.00
32_D36_H2.5781.00
23_R202_Y2.4911.00
32_D67_H2.4681.00
58_K62_D2.4211.00
61_D65_E2.4141.00
12_M19_G2.2971.00
199_K203_E2.2751.00
55_T61_D2.2541.00
47_D115_D2.1811.00
38_V102_L2.1731.00
17_L23_R2.0711.00
24_P206_K2.0471.00
202_Y206_K1.9991.00
5_L96_P1.9981.00
53_I64_L1.8671.00
108_F205_I1.8671.00
135_P139_C1.8401.00
41_S115_D1.8371.00
77_R80_E1.8351.00
196_I205_I1.8261.00
19_G57_D1.8151.00
113_L116_V1.7851.00
189_A192_Y1.7511.00
23_R199_K1.7331.00
29_R63_I1.7171.00
119_Q123_D1.7091.00
46_K115_D1.7011.00
62_D66_A1.6981.00
92_E176_Y1.6761.00
203_E206_K1.6751.00
39_R51_L1.6581.00
98_V121_H1.6471.00
29_R32_D1.6411.00
37_R40_Q1.6331.00
30_L196_I1.6251.00
82_R85_D1.6211.00
189_A193_R1.6211.00
35_V69_I1.5801.00
33_I37_R1.5561.00
102_L109_V1.5061.00
24_P27_S1.5031.00
61_D73_F1.4821.00
16_R153_R1.4631.00
6_F102_L1.4481.00
3_D97_S1.4181.00
7_I90_V1.4121.00
17_L199_K1.4081.00
89_K93_A1.4051.00
100_I169_I1.3971.00
172_H176_Y1.3911.00
41_S47_D1.3761.00
25_L30_L1.3681.00
29_R33_I1.3661.00
33_I209_Y1.3651.00
121_H129_T1.3501.00
52_V94_V1.3031.00
188_R193_R1.3011.00
117_M167_L1.2851.00
117_M169_I1.2831.00
37_R111_P1.2661.00
62_D65_E1.2601.00
4_I122_F1.2331.00
48_R115_D1.2331.00
77_R89_K1.2271.00
63_I66_A1.2061.00
88_V92_E1.1781.00
35_V39_R1.1631.00
61_D75_V1.1581.00
28_N33_I1.1521.00
112_E116_V1.1411.00
120_A129_T1.1331.00
78_G81_E1.1311.00
47_D119_Q1.1271.00
5_L52_V1.1261.00
3_D49_D1.1231.00
53_I69_I1.1211.00
203_E207_A1.1121.00
25_L209_Y1.1041.00
184_P193_R1.0951.00
25_L202_Y1.0821.00
28_N32_D1.0811.00
40_Q111_P1.0721.00
48_R100_I1.0661.00
12_M60_T1.0591.00
4_I98_V1.0551.00
26_G108_F1.0481.00
12_M59_E1.0421.00
91_I172_H1.0391.00
190_P193_R1.0391.00
10_A31_L1.0321.00
22_L63_I1.0281.00
83_V101_R1.0251.00
52_V90_V1.0231.00
207_A211_K1.0211.00
81_E85_D1.0171.00
31_L64_L1.0131.00
7_I54_A1.0091.00
120_A124_Q1.0091.00
117_M129_T1.0001.00
98_V122_F0.9991.00
207_A210_Q0.9991.00
100_I118_I0.9951.00
17_L202_Y0.9891.00
80_E89_K0.9881.00
144_N148_L0.9831.00
186_R193_R0.9811.00
6_F41_S0.9731.00
187_F190_P0.9701.00
12_M57_D0.9701.00
133_N159_D0.9691.00
58_K61_D0.9641.00
106_N195_T0.9521.00
206_K210_Q0.9431.00
3_D50_N0.9381.00
59_E62_D0.9331.00
23_R206_K0.9271.00
25_L205_I0.9271.00
83_V103_T0.9251.00
26_G210_Q0.9211.00
184_P187_F0.9191.00
38_V51_L0.9151.00
8_I38_V0.9111.00
115_D119_Q0.9091.00
88_V176_Y0.9051.00
16_R152_S0.8951.00
41_S48_R0.8931.00
64_L68_C0.8841.00
173_P177_R0.8841.00
117_M131_I0.8801.00
87_F101_R0.8791.00
50_N94_V0.8741.00
85_D89_K0.8681.00
69_I73_F0.8561.00
52_V70_K0.8561.00
204_S208_L0.8471.00
35_V67_H0.8461.00
129_T169_I0.8451.00
194_L205_I0.8390.99
116_V119_Q0.8380.99
65_E70_K0.8370.99
95_K172_H0.8290.99
36_H39_R0.8250.99
63_I67_H0.8210.99
58_K65_E0.8200.99
24_P29_R0.8180.99
4_I118_I0.8180.99
184_P190_P0.8170.99
78_G89_K0.8150.99
204_S207_A0.8110.99
76_F89_K0.8050.99
128_Y174_A0.8020.99
10_A104_G0.8000.99
47_D118_I0.7980.99
83_V168_Y0.7970.99
135_P141_E0.7960.99
109_V114_L0.7910.99
185_E189_A0.7890.99
32_D35_V0.7890.99
186_R189_A0.7870.99
108_F209_Y0.7870.99
118_I169_I0.7860.99
185_E188_R0.7810.99
55_T60_T0.7790.99
41_S46_K0.7750.99
131_I167_L0.7740.99
121_H169_I0.7740.99
8_I35_V0.7720.99
120_A123_D0.7720.99
142_V145_A0.7680.99
132_L163_E0.7590.99
187_F193_R0.7550.99
110_D113_L0.7540.99
27_S33_I0.7460.99
7_I52_V0.7420.99
111_P115_D0.7360.99
32_D63_I0.7360.99
5_L50_N0.7330.99
74_R90_V0.7330.99
9_Q83_V0.7320.99
82_R162_Q0.7310.99
113_L192_Y0.7290.99
6_F51_L0.7290.99
55_T73_F0.7260.99
47_D122_F0.7240.99
37_R109_V0.7240.99
57_D60_T0.7220.98
88_V179_Q0.7210.98
52_V74_R0.7120.98
9_Q101_R0.7090.98
152_S156_A0.7090.98
21_V60_T0.7080.98
200_E204_S0.7070.98
58_K75_V0.7030.98
35_V53_I0.7030.98
163_E195_T0.7020.98
126_S139_C0.6950.98
83_V86_R0.6940.98
26_G209_Y0.6910.98
33_I36_H0.6900.98
103_T165_V0.6830.98
113_L131_I0.6800.98
7_I99_I0.6760.98
68_C73_F0.6720.98
164_H188_R0.6650.97
184_P189_A0.6640.97
65_E73_F0.6640.97
194_L204_S0.6600.97
64_L67_H0.6550.97
147_L150_D0.6510.97
26_G206_K0.6470.97
10_A21_V0.6460.97
121_H171_N0.6450.97
36_H40_Q0.6440.97
81_E86_R0.6420.97
41_S118_I0.6380.97
21_V30_L0.6370.97
34_L109_V0.6340.97
128_Y170_R0.6330.97
74_R77_R0.6330.97
145_A149_I0.6330.97
3_D95_K0.6330.97
26_G33_I0.6330.97
5_L97_S0.6260.96
51_L69_I0.6220.96
135_P138_I0.6220.96
200_E203_E0.6220.96
174_A178_V0.6200.96
99_I172_H0.6150.96
12_M21_V0.6130.96
50_N96_P0.6110.96
98_V169_I0.6100.96
41_S114_L0.6080.96
144_N147_L0.6050.96
103_T106_N0.6040.95
179_Q182_E0.6020.95
98_V118_I0.5980.95
9_Q103_T0.5960.95
34_L38_V0.5950.95
50_N74_R0.5930.95
53_I68_C0.5920.95
151_I161_Y0.5910.95
176_Y180_F0.5880.95
151_I156_A0.5870.95
4_I49_D0.5870.95
96_P99_I0.5860.95
35_V64_L0.5840.95
9_Q87_F0.5820.94
191_Q200_E0.5810.94
21_V31_L0.5800.94
153_R188_R0.5800.94
114_L167_L0.5800.94
48_R118_I0.5790.94
142_V148_L0.5790.94
135_P140_G0.5760.94
90_V94_V0.5760.94
34_L37_R0.5710.94
146_N150_D0.5700.94
184_P188_R0.5680.94
85_D152_S0.5680.94
116_V120_A0.5670.94
117_M121_H0.5670.94
103_T168_Y0.5620.93
91_I99_I0.5600.93
203_E211_K0.5550.93
185_E193_R0.5530.93
59_E63_I0.5510.93
184_P191_Q0.5490.93
102_L114_L0.5480.93
7_I101_R0.5470.92
113_L167_L0.5460.92
74_R93_A0.5450.92
8_I34_L0.5430.92
150_D153_R0.5410.92
190_P198_T0.5410.92
128_Y131_I0.5390.92
4_I48_R0.5390.92
60_T63_I0.5340.92
11_R14_S0.5340.92
180_F183_A0.5290.91
126_S184_P0.5280.91
5_L94_V0.5260.91
178_V182_E0.5250.91
83_V166_T0.5210.91
46_K119_Q0.5210.91
54_A89_K0.5200.91
118_I122_F0.5170.90
121_H126_S0.5140.90
103_T164_H0.5110.90
12_M56_S0.5110.90
170_R175_L0.5100.90
108_F194_L0.5080.89
153_R156_A0.5080.89
127_D171_N0.5060.89
41_S100_I0.5040.89
91_I176_Y0.5030.89
158_E161_Y0.5000.89
115_D118_I0.5000.89
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1eziA 2 0.8333 99.9 0.567 Contact Map
1qwjA 2 0.8708 99.9 0.569 Contact Map
3k8dA 2 0.8333 99.9 0.579 Contact Map
1h7eA 2 0.8667 99.9 0.582 Contact Map
4xwiA 2 0.8458 99.9 0.584 Contact Map
3oamA 2 0.8583 99.9 0.584 Contact Map
4fcuA 2 0.8583 99.9 0.596 Contact Map
1vicA 2 0.8542 99.9 0.598 Contact Map
3tqdA 2 0.8125 99.9 0.6 Contact Map
2y6pA 2 0.8292 99.9 0.604 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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