SPSF - BACSU - GREMLIN Contacts

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SPSF - Spore coat polysaccharide biosynthesis protein SpsF

UniProt:	P39626	Sequences: MNDILFIIQARMGSTRLPGKVLRPLGSNRLLDILVHRVRQSAFYQKDRDNLVIATSDKETDDILEAHCIKQGFRVFRGSEERVLDRFVKVIEAVKPSVIIRLTGDNPFVDPELLDVMIQAHFDQGSDYTYILNAPLGICGEVVNANLLIDISRIQALEDQYQEHVTLYIRNHPALYRVQFLEAPERFRAPQYRLTIDTKEDYESIKALYQKAGERPDVSASELITLLNRNPDAATEREAD	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	240 (201)
Sequences:	4837 (3792)
Seq/√Len:	267.5

ECOLI - KDSB
BACSU - SPSF

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
28_N	36_H	4.100	1.00
50_N	70_K	3.788	1.00
6_F	48_R	2.812	1.00
19_G	59_E	2.739	1.00
64_L	69_I	2.607	1.00
32_D	36_H	2.578	1.00
23_R	202_Y	2.491	1.00
32_D	67_H	2.468	1.00
58_K	62_D	2.421	1.00
61_D	65_E	2.414	1.00
12_M	19_G	2.297	1.00
199_K	203_E	2.275	1.00
55_T	61_D	2.254	1.00
47_D	115_D	2.181	1.00
38_V	102_L	2.173	1.00
17_L	23_R	2.071	1.00
24_P	206_K	2.047	1.00
202_Y	206_K	1.999	1.00
5_L	96_P	1.998	1.00
53_I	64_L	1.867	1.00
108_F	205_I	1.867	1.00
135_P	139_C	1.840	1.00
41_S	115_D	1.837	1.00
77_R	80_E	1.835	1.00
196_I	205_I	1.826	1.00
19_G	57_D	1.815	1.00
113_L	116_V	1.785	1.00
189_A	192_Y	1.751	1.00
23_R	199_K	1.733	1.00
29_R	63_I	1.717	1.00
119_Q	123_D	1.709	1.00
46_K	115_D	1.701	1.00
62_D	66_A	1.698	1.00
92_E	176_Y	1.676	1.00
203_E	206_K	1.675	1.00
39_R	51_L	1.658	1.00
98_V	121_H	1.647	1.00
29_R	32_D	1.641	1.00
37_R	40_Q	1.633	1.00
30_L	196_I	1.625	1.00
82_R	85_D	1.621	1.00
189_A	193_R	1.621	1.00
35_V	69_I	1.580	1.00
33_I	37_R	1.556	1.00
102_L	109_V	1.506	1.00
24_P	27_S	1.503	1.00
61_D	73_F	1.482	1.00
16_R	153_R	1.463	1.00
6_F	102_L	1.448	1.00
3_D	97_S	1.418	1.00
7_I	90_V	1.412	1.00
17_L	199_K	1.408	1.00
89_K	93_A	1.405	1.00
100_I	169_I	1.397	1.00
172_H	176_Y	1.391	1.00
41_S	47_D	1.376	1.00
25_L	30_L	1.368	1.00
29_R	33_I	1.366	1.00
33_I	209_Y	1.365	1.00
121_H	129_T	1.350	1.00
52_V	94_V	1.303	1.00
188_R	193_R	1.301	1.00
117_M	167_L	1.285	1.00
117_M	169_I	1.283	1.00
37_R	111_P	1.266	1.00
62_D	65_E	1.260	1.00
4_I	122_F	1.233	1.00
48_R	115_D	1.233	1.00
77_R	89_K	1.227	1.00
63_I	66_A	1.206	1.00
88_V	92_E	1.178	1.00
35_V	39_R	1.163	1.00
61_D	75_V	1.158	1.00
28_N	33_I	1.152	1.00
112_E	116_V	1.141	1.00
120_A	129_T	1.133	1.00
78_G	81_E	1.131	1.00
47_D	119_Q	1.127	1.00
5_L	52_V	1.126	1.00
3_D	49_D	1.123	1.00
53_I	69_I	1.121	1.00
203_E	207_A	1.112	1.00
25_L	209_Y	1.104	1.00
184_P	193_R	1.095	1.00
25_L	202_Y	1.082	1.00
28_N	32_D	1.081	1.00
40_Q	111_P	1.072	1.00
48_R	100_I	1.066	1.00
12_M	60_T	1.059	1.00
4_I	98_V	1.055	1.00
26_G	108_F	1.048	1.00
12_M	59_E	1.042	1.00
91_I	172_H	1.039	1.00
190_P	193_R	1.039	1.00
10_A	31_L	1.032	1.00
22_L	63_I	1.028	1.00
83_V	101_R	1.025	1.00
52_V	90_V	1.023	1.00
207_A	211_K	1.021	1.00
81_E	85_D	1.017	1.00
31_L	64_L	1.013	1.00
7_I	54_A	1.009	1.00
120_A	124_Q	1.009	1.00
117_M	129_T	1.000	1.00
98_V	122_F	0.999	1.00
207_A	210_Q	0.999	1.00
100_I	118_I	0.995	1.00
17_L	202_Y	0.989	1.00
80_E	89_K	0.988	1.00
144_N	148_L	0.983	1.00
186_R	193_R	0.981	1.00
6_F	41_S	0.973	1.00
187_F	190_P	0.970	1.00
12_M	57_D	0.970	1.00
133_N	159_D	0.969	1.00
58_K	61_D	0.964	1.00
106_N	195_T	0.952	1.00
206_K	210_Q	0.943	1.00
3_D	50_N	0.938	1.00
59_E	62_D	0.933	1.00
23_R	206_K	0.927	1.00
25_L	205_I	0.927	1.00
83_V	103_T	0.925	1.00
26_G	210_Q	0.921	1.00
184_P	187_F	0.919	1.00
38_V	51_L	0.915	1.00
8_I	38_V	0.911	1.00
115_D	119_Q	0.909	1.00
88_V	176_Y	0.905	1.00
16_R	152_S	0.895	1.00
41_S	48_R	0.893	1.00
64_L	68_C	0.884	1.00
173_P	177_R	0.884	1.00
117_M	131_I	0.880	1.00
87_F	101_R	0.879	1.00
50_N	94_V	0.874	1.00
85_D	89_K	0.868	1.00
69_I	73_F	0.856	1.00
52_V	70_K	0.856	1.00
204_S	208_L	0.847	1.00
35_V	67_H	0.846	1.00
129_T	169_I	0.845	1.00
194_L	205_I	0.839	0.99
116_V	119_Q	0.838	0.99
65_E	70_K	0.837	0.99
95_K	172_H	0.829	0.99
36_H	39_R	0.825	0.99
63_I	67_H	0.821	0.99
58_K	65_E	0.820	0.99
24_P	29_R	0.818	0.99
4_I	118_I	0.818	0.99
184_P	190_P	0.817	0.99
78_G	89_K	0.815	0.99
204_S	207_A	0.811	0.99
76_F	89_K	0.805	0.99
128_Y	174_A	0.802	0.99
10_A	104_G	0.800	0.99
47_D	118_I	0.798	0.99
83_V	168_Y	0.797	0.99
135_P	141_E	0.796	0.99
109_V	114_L	0.791	0.99
185_E	189_A	0.789	0.99
32_D	35_V	0.789	0.99
186_R	189_A	0.787	0.99
108_F	209_Y	0.787	0.99
118_I	169_I	0.786	0.99
185_E	188_R	0.781	0.99
55_T	60_T	0.779	0.99
41_S	46_K	0.775	0.99
131_I	167_L	0.774	0.99
121_H	169_I	0.774	0.99
8_I	35_V	0.772	0.99
120_A	123_D	0.772	0.99
142_V	145_A	0.768	0.99
132_L	163_E	0.759	0.99
187_F	193_R	0.755	0.99
110_D	113_L	0.754	0.99
27_S	33_I	0.746	0.99
7_I	52_V	0.742	0.99
111_P	115_D	0.736	0.99
32_D	63_I	0.736	0.99
5_L	50_N	0.733	0.99
74_R	90_V	0.733	0.99
9_Q	83_V	0.732	0.99
82_R	162_Q	0.731	0.99
113_L	192_Y	0.729	0.99
6_F	51_L	0.729	0.99
55_T	73_F	0.726	0.99
47_D	122_F	0.724	0.99
37_R	109_V	0.724	0.99
57_D	60_T	0.722	0.98
88_V	179_Q	0.721	0.98
52_V	74_R	0.712	0.98
9_Q	101_R	0.709	0.98
152_S	156_A	0.709	0.98
21_V	60_T	0.708	0.98
200_E	204_S	0.707	0.98
58_K	75_V	0.703	0.98
35_V	53_I	0.703	0.98
163_E	195_T	0.702	0.98
126_S	139_C	0.695	0.98
83_V	86_R	0.694	0.98
26_G	209_Y	0.691	0.98
33_I	36_H	0.690	0.98
103_T	165_V	0.683	0.98
113_L	131_I	0.680	0.98
7_I	99_I	0.676	0.98
68_C	73_F	0.672	0.98
164_H	188_R	0.665	0.97
184_P	189_A	0.664	0.97
65_E	73_F	0.664	0.97
194_L	204_S	0.660	0.97
64_L	67_H	0.655	0.97
147_L	150_D	0.651	0.97
26_G	206_K	0.647	0.97
10_A	21_V	0.646	0.97
121_H	171_N	0.645	0.97
36_H	40_Q	0.644	0.97
81_E	86_R	0.642	0.97
41_S	118_I	0.638	0.97
21_V	30_L	0.637	0.97
34_L	109_V	0.634	0.97
128_Y	170_R	0.633	0.97
74_R	77_R	0.633	0.97
145_A	149_I	0.633	0.97
3_D	95_K	0.633	0.97
26_G	33_I	0.633	0.97
5_L	97_S	0.626	0.96
51_L	69_I	0.622	0.96
135_P	138_I	0.622	0.96
200_E	203_E	0.622	0.96
174_A	178_V	0.620	0.96
99_I	172_H	0.615	0.96
12_M	21_V	0.613	0.96
50_N	96_P	0.611	0.96
98_V	169_I	0.610	0.96
41_S	114_L	0.608	0.96
144_N	147_L	0.605	0.96
103_T	106_N	0.604	0.95
179_Q	182_E	0.602	0.95
98_V	118_I	0.598	0.95
9_Q	103_T	0.596	0.95
34_L	38_V	0.595	0.95
50_N	74_R	0.593	0.95
53_I	68_C	0.592	0.95
151_I	161_Y	0.591	0.95
176_Y	180_F	0.588	0.95
151_I	156_A	0.587	0.95
4_I	49_D	0.587	0.95
96_P	99_I	0.586	0.95
35_V	64_L	0.584	0.95
9_Q	87_F	0.582	0.94
191_Q	200_E	0.581	0.94
21_V	31_L	0.580	0.94
153_R	188_R	0.580	0.94
114_L	167_L	0.580	0.94
48_R	118_I	0.579	0.94
142_V	148_L	0.579	0.94
135_P	140_G	0.576	0.94
90_V	94_V	0.576	0.94
34_L	37_R	0.571	0.94
146_N	150_D	0.570	0.94
184_P	188_R	0.568	0.94
85_D	152_S	0.568	0.94
116_V	120_A	0.567	0.94
117_M	121_H	0.567	0.94
103_T	168_Y	0.562	0.93
91_I	99_I	0.560	0.93
203_E	211_K	0.555	0.93
185_E	193_R	0.553	0.93
59_E	63_I	0.551	0.93
184_P	191_Q	0.549	0.93
102_L	114_L	0.548	0.93
7_I	101_R	0.547	0.92
113_L	167_L	0.546	0.92
74_R	93_A	0.545	0.92
8_I	34_L	0.543	0.92
150_D	153_R	0.541	0.92
190_P	198_T	0.541	0.92
128_Y	131_I	0.539	0.92
4_I	48_R	0.539	0.92
60_T	63_I	0.534	0.92
11_R	14_S	0.534	0.92
180_F	183_A	0.529	0.91
126_S	184_P	0.528	0.91
5_L	94_V	0.526	0.91
178_V	182_E	0.525	0.91
83_V	166_T	0.521	0.91
46_K	119_Q	0.521	0.91
54_A	89_K	0.520	0.91
118_I	122_F	0.517	0.90
121_H	126_S	0.514	0.90
103_T	164_H	0.511	0.90
12_M	56_S	0.511	0.90
170_R	175_L	0.510	0.90
108_F	194_L	0.508	0.89
153_R	156_A	0.508	0.89
127_D	171_N	0.506	0.89
41_S	100_I	0.504	0.89
91_I	176_Y	0.503	0.89
158_E	161_Y	0.500	0.89
115_D	118_I	0.500	0.89

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1eziA	2	0.8333	99.9	0.567	Contact Map
1qwjA	2	0.8708	99.9	0.569	Contact Map
3k8dA	2	0.8333	99.9	0.579	Contact Map
1h7eA	2	0.8667	99.9	0.582	Contact Map
4xwiA	2	0.8458	99.9	0.584	Contact Map
3oamA	2	0.8583	99.9	0.584	Contact Map
4fcuA	2	0.8583	99.9	0.596	Contact Map
1vicA	2	0.8542	99.9	0.598	Contact Map
3tqdA	2	0.8125	99.9	0.6	Contact Map
2y6pA	2	0.8292	99.9	0.604	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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