GREMLIN Database
SPSD - Spore coat polysaccharide biosynthesis protein SpsD
UniProt: P39624 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 289 (227)
Sequences: 249 (200)
Seq/√Len: 13.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
157_S221_A4.7561.00
51_W162_R3.6820.99
232_A266_Y3.5850.99
180_E184_N3.0140.97
202_V264_R3.0130.97
65_L95_V2.9320.96
118_G280_Y2.9250.96
140_G197_K2.7330.95
220_M262_A2.5430.92
262_A266_Y2.4870.91
51_W55_V2.4640.91
158_F174_A2.4350.90
204_G231_I2.3790.89
59_K91_D2.3280.88
182_T207_Q2.2370.86
181_W219_L2.1970.85
233_F268_R2.1880.84
232_A262_A2.1370.83
121_L233_F2.0040.79
150_I186_L1.9990.79
118_G281_H1.9230.76
56_L178_F1.9160.75
96_R279_Y1.9060.75
161_S232_A1.9030.75
161_S266_Y1.8900.74
195_V198_H1.8430.72
178_F181_W1.8330.72
140_G264_R1.8210.71
161_S262_A1.8150.71
53_S59_K1.8070.71
53_S235_L1.7980.70
217_L253_A1.7630.69
150_I182_T1.7460.68
113_S282_I1.7240.67
94_F279_Y1.7170.67
105_A280_Y1.7080.66
166_D281_H1.6980.66
55_V162_R1.6790.65
53_S58_K1.6730.65
207_Q240_I1.6690.65
128_S132_K1.6660.64
86_Q213_D1.6560.64
235_L255_T1.6490.64
218_D262_A1.6340.63
224_P227_E1.5750.60
61_M90_T1.5610.59
221_A238_N1.5500.59
94_F162_R1.5060.56
62_N222_V1.4810.55
219_L256_Q1.4670.54
207_Q218_D1.4500.53
205_Y219_L1.4410.53
170_S173_A1.4330.52
96_R120_L1.4250.52
58_K249_R1.4240.52
154_A221_A1.4040.51
55_V163_Y1.4020.51
53_S60_V1.3940.50
177_I255_T1.3930.50
162_R266_Y1.3820.50
49_S262_A1.3800.50
253_A256_Q1.3780.50
198_H201_E1.3730.49
232_A265_L1.3600.49
123_L263_I1.3470.48
125_M240_I1.3400.48
219_L255_T1.3290.47
120_L158_F1.3110.46
120_L177_I1.3110.46
55_V266_Y1.2840.45
48_D192_V1.2610.43
85_R264_R1.2430.42
167_P239_L1.2270.42
157_S231_I1.2260.42
218_D254_G1.2200.41
120_L279_Y1.2110.41
184_N187_N1.2060.41
115_Y264_R1.2010.40
119_S281_H1.1940.40
131_D172_D1.1920.40
122_K254_G1.1910.40
139_L272_T1.1720.39
203_I211_R1.1660.39
190_A281_H1.1580.38
47_E146_D1.1530.38
44_L95_V1.1440.37
55_V262_A1.1420.37
98_P103_G1.1390.37
177_I262_A1.1320.37
256_Q266_Y1.1300.37
63_V90_T1.1260.37
51_W163_Y1.1260.37
132_K245_T1.1250.37
60_V158_F1.1250.37
233_F269_M1.1220.36
185_N190_A1.1160.36
161_S217_L1.1150.36
59_K222_V1.1140.36
94_F165_Q1.1140.36
89_E144_P1.1130.36
90_T231_I1.1110.36
57_Q144_P1.1030.35
55_V177_I1.0970.35
154_A205_Y1.0770.34
221_A282_I1.0650.34
168_H281_H1.0650.34
85_R247_K1.0600.33
161_S256_Q1.0550.33
49_S255_T1.0540.33
55_V169_L1.0500.33
177_I256_Q1.0440.33
118_G168_H1.0390.32
91_D127_P1.0350.32
169_L173_A1.0330.32
162_R177_I1.0180.31
206_L262_A1.0160.31
263_I273_A1.0140.31
95_V280_Y1.0140.31
225_G229_K1.0050.31
99_A229_K0.9990.31
101_D104_A0.9960.30
217_L266_Y0.9910.30
154_A161_S0.9810.30
93_I240_I0.9730.29
256_Q262_A0.9700.29
162_R232_A0.9700.29
229_K258_H0.9690.29
253_A262_A0.9590.29
139_L197_K0.9570.29
210_S246_Q0.9560.29
133_T212_D0.9550.29
161_S253_A0.9550.29
158_F177_I0.9540.29
255_T262_A0.9480.28
49_S177_I0.9480.28
55_V279_Y0.9410.28
139_L264_R0.9400.28
157_S213_D0.9390.28
54_K102_I0.9370.28
103_G110_Q0.9320.28
49_S55_V0.9280.28
150_I268_R0.9270.27
60_V282_I0.9130.27
82_Y251_V0.9120.27
151_C183_R0.9030.26
182_T193_N0.8900.26
57_Q160_K0.8830.26
217_L256_Q0.8820.26
50_L199_N0.8710.25
132_K172_D0.8700.25
185_N209_L0.8670.25
120_L254_G0.8650.25
49_S256_Q0.8630.25
62_N182_T0.8580.25
98_P101_D0.8570.25
61_M149_A0.8560.25
55_V256_Q0.8530.24
172_D202_V0.8520.24
52_E207_Q0.8520.24
83_T248_H0.8510.24
63_V162_R0.8510.24
123_L267_E0.8460.24
254_G262_A0.8450.24
85_R115_Y0.8360.24
217_L251_V0.8310.24
196_A234_H0.8290.24
121_L223_K0.8230.23
217_L220_M0.8190.23
161_S218_D0.8190.23
203_I226_F0.8180.23
130_Y139_L0.8170.23
153_L157_S0.8150.23
59_K83_T0.8110.23
70_S280_Y0.8040.23
144_P152_E0.7990.22
120_L163_Y0.7980.22
202_V261_R0.7980.22
52_E151_C0.7960.22
166_D279_Y0.7770.22
206_L217_L0.7770.22
101_D149_A0.7710.21
220_M242_Q0.7690.21
142_P158_F0.7690.21
62_N117_V0.7650.21
177_I186_L0.7630.21
162_R279_Y0.7620.21
139_L145_G0.7560.21
149_A195_V0.7530.21
49_S216_I0.7500.21
91_D211_R0.7470.21
150_I267_E0.7400.20
134_P260_V0.7390.20
204_G260_V0.7350.20
92_F265_L0.7340.20
241_E246_Q0.7330.20
51_W232_A0.7260.20
133_T141_P0.7210.20
249_R275_Q0.7190.20
120_L262_A0.7180.20
94_F158_F0.7170.20
169_L254_G0.7140.19
56_L185_N0.7070.19
191_A218_D0.7060.19
161_S281_H0.7050.19
153_L223_K0.7040.19
168_H279_Y0.7000.19
55_V255_T0.6970.19
54_K193_N0.6960.19
142_P167_P0.6920.19
63_V152_E0.6900.19
57_Q86_Q0.6880.19
234_H247_K0.6880.19
232_A235_L0.6790.18
68_A156_D0.6760.18
167_P256_Q0.6730.18
131_D274_E0.6730.18
162_R166_D0.6720.18
51_W266_Y0.6720.18
115_Y121_L0.6630.18
140_G234_H0.6630.18
110_Q197_K0.6630.18
156_D196_A0.6620.18
59_K124_A0.6600.18
204_G264_R0.6580.18
254_G279_Y0.6580.18
204_G266_Y0.6580.18
96_R113_S0.6570.18
211_R267_E0.6570.18
69_N225_G0.6570.18
236_L256_Q0.6570.18
158_F181_W0.6570.18
186_L190_A0.6560.18
152_E199_N0.6530.18
49_S163_Y0.6510.17
182_T238_N0.6500.17
193_N207_Q0.6460.17
204_G262_A0.6440.17
64_K109_Q0.6430.17
194_I216_I0.6400.17
151_C246_Q0.6380.17
258_H280_Y0.6380.17
176_E262_A0.6370.17
192_V208_G0.6360.17
117_V263_I0.6340.17
174_A177_I0.6290.17
56_L163_Y0.6280.17
264_R272_T0.6270.17
118_G169_L0.6270.17
205_Y230_R0.6270.17
112_P282_I0.6260.17
173_A176_E0.6210.17
141_P241_E0.6210.17
153_L158_F0.6210.17
119_S279_Y0.6170.16
191_A255_T0.6150.16
57_Q184_N0.6130.16
177_I254_G0.6120.16
197_K204_G0.6120.16
201_E268_R0.6100.16
194_I198_H0.6100.16
260_V268_R0.6090.16
217_L262_A0.6050.16
176_E194_I0.6020.16
177_I190_A0.6020.16
110_Q198_H0.5990.16
127_P193_N0.5990.16
111_Q125_M0.5980.16
203_I233_F0.5980.16
94_F281_H0.5970.16
254_G281_H0.5950.16
113_S142_P0.5940.16
114_S248_H0.5880.16
44_L65_L0.5860.16
59_K231_I0.5860.16
67_A247_K0.5850.16
94_F174_A0.5830.15
105_A136_F0.5820.15
68_A196_A0.5780.15
206_L255_T0.5770.15
234_H280_Y0.5750.15
117_V212_D0.5740.15
88_D144_P0.5730.15
91_D156_D0.5730.15
160_K269_M0.5720.15
131_D149_A0.5720.15
133_T143_E0.5710.15
209_L243_P0.5690.15
163_Y209_L0.5660.15
148_E249_R0.5600.15
84_V247_K0.5590.15
271_F281_H0.5570.15
167_P255_T0.5540.15
206_L256_Q0.5540.15
98_P104_A0.5510.15
64_K225_G0.5500.15
120_L232_A0.5490.15
60_V114_S0.5450.14
176_E189_R0.5440.14
118_G171_R0.5430.14
92_F254_G0.5420.14
56_L175_N0.5410.14
60_V92_F0.5390.14
139_L207_Q0.5380.14
119_S209_L0.5380.14
120_L218_D0.5370.14
99_A276_S0.5350.14
101_D171_R0.5300.14
123_L266_Y0.5290.14
141_P193_N0.5290.14
97_V278_Y0.5280.14
58_K231_I0.5270.14
238_N270_G0.5260.14
181_W205_Y0.5260.14
236_L255_T0.5240.14
46_Y102_I0.5220.14
178_F218_D0.5220.14
200_G209_L0.5190.14
202_V233_F0.5180.14
195_V245_T0.5180.14
124_A143_E0.5170.14
242_Q271_F0.5160.14
105_A109_Q0.5150.14
252_T274_E0.5150.14
55_V117_V0.5140.14
82_Y154_A0.5130.14
150_I276_S0.5120.14
196_A217_L0.5120.14
178_F219_L0.5110.14
59_K84_V0.5110.14
233_F260_V0.5100.14
197_K247_K0.5090.14
96_R165_Q0.5070.13
162_R263_I0.5070.13
93_I179_Q0.5070.13
56_L259_N0.5060.13
108_I182_T0.5050.13
109_Q260_V0.5050.13
188_G191_A0.5040.13
122_K235_L0.5040.13
50_L152_E0.5000.13
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2ft0A 2 0.7578 100 0.648 Contact Map
3tt2A 1 0.9308 99.9 0.718 Contact Map
1p0hA 1 0.8997 99.8 0.74 Contact Map
2wpxA 1 0.91 99.8 0.742 Contact Map
1ufhA 3 0.5087 99.8 0.756 Contact Map
1s3zA 2 0.481 99.8 0.757 Contact Map
1vkcA 2 0.4913 99.8 0.759 Contact Map
3frmA 3 0.8512 99.8 0.759 Contact Map
1on0A 4 0.5156 99.8 0.761 Contact Map
3owcA 1 0.5294 99.8 0.761 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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