GREMLIN Database
SPSB - Spore coat polysaccharide biosynthesis protein SpsB
UniProt: P39622 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 474 (394)
Sequences: 548 (518)
Seq/√Len: 26.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
450_A456_L7.0201.00
414_Q417_E6.3221.00
455_A459_E5.9611.00
229_L269_H5.2301.00
300_L365_L4.0631.00
412_F421_T3.8321.00
246_F269_H3.7451.00
311_Y314_V3.6391.00
452_S455_A3.5621.00
251_A266_V3.4421.00
392_I409_D3.2671.00
245_V251_A3.1931.00
184_T187_A2.9061.00
303_T375_Q2.8461.00
142_S145_Y2.7971.00
304_Q375_Q2.6700.99
233_E240_T2.5300.99
225_Q229_L2.4000.99
306_F396_S2.3970.99
394_K411_M2.2550.98
303_T306_F2.2360.98
306_F375_Q2.2110.98
304_Q378_T2.2060.98
149_A158_D2.1720.97
270_P457_F2.1110.97
297_K370_D2.0870.96
247_G250_E2.0470.96
225_Q250_E1.9860.95
142_S201_T1.9810.95
226_H304_Q1.9760.95
148_T152_L1.9440.95
304_Q331_H1.8910.94
248_A252_E1.8800.94
179_I182_I1.8600.93
68_P82_D1.8300.93
305_P375_Q1.8060.92
378_T381_L1.8040.92
215_Q241_T1.7850.92
250_E269_H1.7730.92
304_Q376_T1.7620.91
228_A269_H1.7320.91
226_H377_S1.7230.90
306_F376_T1.6860.89
196_A220_V1.6800.89
312_S316_Q1.6790.89
142_S146_L1.6760.89
138_Q143_K1.6730.89
226_H306_F1.6610.89
50_E53_L1.6550.88
297_K324_L1.6510.88
298_I325_Q1.6500.88
227_G305_P1.6480.88
162_F299_V1.6460.88
229_L246_F1.6450.88
271_R374_T1.6420.88
229_L250_E1.6190.87
374_T409_D1.6130.87
319_S422_L1.6110.87
233_E237_P1.5840.86
141_D144_Q1.5580.85
228_A246_F1.5570.85
391_L412_F1.5400.84
128_I133_I1.5140.83
233_E239_F1.5130.83
303_T376_T1.5120.83
413_D417_E1.5040.83
64_G74_I1.4970.83
124_D128_I1.4910.82
92_H99_S1.4860.82
331_H377_S1.4850.82
236_I240_T1.4790.82
129_S132_E1.4780.82
325_Q354_R1.4690.81
375_Q409_D1.4670.81
316_Q320_D1.4660.81
242_Y265_L1.4650.81
135_D142_S1.4610.81
243_Q254_F1.4590.81
233_E236_I1.4560.81
49_E54_F1.4540.81
65_Q76_N1.4480.81
304_Q396_S1.4330.80
248_A266_V1.4320.80
250_E254_F1.4290.80
249_Y253_W1.4270.80
126_P133_I1.4250.79
260_K263_Q1.4090.79
303_T378_T1.4050.78
185_V188_L1.3960.78
209_V213_M1.3930.78
50_E57_V1.3860.77
225_Q269_H1.3790.77
375_Q378_T1.3720.77
159_H162_F1.3700.77
48_E52_E1.3690.77
371_A391_L1.3610.76
49_E53_L1.3550.76
62_D65_Q1.3470.75
298_I327_I1.3430.75
298_I302_A1.3380.75
258_G367_P1.3360.75
223_C269_H1.3260.74
51_P56_S1.3260.74
433_Q436_E1.3210.74
403_Y407_L1.3160.74
248_A447_Y1.3150.74
303_T382_E1.2930.72
381_L446_N1.2930.72
272_F382_E1.2880.72
231_G269_H1.2880.72
299_V414_Q1.2850.72
49_E52_E1.2800.72
228_A254_F1.2780.71
443_I446_N1.2730.71
114_L117_W1.2730.71
73_R76_N1.2710.71
271_R303_T1.2630.71
64_G69_F1.2590.70
327_I356_I1.2540.70
226_H331_H1.2540.70
273_D451_E1.2510.70
145_Y149_A1.2500.70
63_E66_I1.2480.70
245_V264_I1.2470.70
47_M52_E1.2420.69
47_M50_E1.2410.69
235_F240_T1.2360.69
223_C304_Q1.2320.69
237_P240_T1.2240.68
226_H305_P1.2230.68
245_V272_F1.2170.68
388_K418_L1.2170.68
372_A383_A1.2140.67
142_S224_L1.2050.67
246_F375_Q1.2020.67
325_Q355_V1.2010.67
306_F378_T1.1960.66
251_A261_P1.1960.66
365_L369_V1.1930.66
319_S352_A1.1930.66
47_M51_P1.1930.66
273_D389_P1.1910.66
142_S160_P1.1900.66
200_G250_E1.1830.65
231_G400_S1.1830.65
225_Q301_I1.1700.64
304_Q374_T1.1670.64
115_A118_S1.1650.64
151_S420_H1.1610.64
271_R306_F1.1580.64
372_A379_V1.1550.63
173_K177_L1.1500.63
303_T396_S1.1490.63
65_Q69_F1.1460.63
143_K272_F1.1410.62
384_M408_G1.1390.62
372_A409_D1.1380.62
252_E344_H1.1370.62
229_L254_F1.1300.62
302_A372_A1.1290.62
296_K325_Q1.1210.61
375_Q396_S1.1180.61
415_P419_A1.1160.61
305_P374_T1.1010.60
248_A251_A1.1000.60
69_F76_N1.0990.60
141_D146_L1.0980.59
329_K335_I1.0960.59
232_D304_Q1.0850.59
50_E58_D1.0780.58
161_A356_I1.0740.58
269_H302_A1.0690.57
226_H378_T1.0670.57
331_H378_T1.0670.57
208_R254_F1.0650.57
366_L384_M1.0640.57
123_C131_R1.0620.57
48_E53_L1.0540.56
50_E54_F1.0530.56
271_R275_I1.0500.56
226_H376_T1.0500.56
52_E56_S1.0480.56
64_G76_N1.0460.56
51_P54_F1.0450.56
305_P378_T1.0390.55
267_T457_F1.0360.55
298_I372_A1.0360.55
392_I400_S1.0330.55
55_G58_D1.0300.55
125_L132_E1.0290.54
363_Y400_S1.0280.54
53_L56_S1.0270.54
246_F250_E1.0180.54
331_H375_Q1.0130.53
50_E56_S1.0100.53
129_S133_I1.0030.53
460_L463_K1.0010.52
450_A453_T0.9960.52
384_M390_V0.9940.52
47_M53_L0.9920.52
126_P129_S0.9920.52
83_E103_Y0.9850.51
210_L382_E0.9820.51
69_F81_N0.9820.51
175_I182_I0.9810.51
143_K435_A0.9810.51
243_Q302_A0.9800.51
298_I369_V0.9770.51
123_C128_I0.9760.51
327_I368_Y0.9750.50
125_L130_K0.9690.50
225_Q272_F0.9670.50
48_E51_P0.9600.49
380_G392_I0.9590.49
143_K407_L0.9540.49
381_L386_F0.9520.49
126_P132_E0.9510.49
298_I368_Y0.9490.49
150_C153_L0.9490.49
138_Q142_S0.9480.48
130_K133_I0.9440.48
453_T456_L0.9380.48
271_R375_Q0.9360.48
62_D82_D0.9340.47
330_P384_M0.9330.47
223_C378_T0.9320.47
231_G272_F0.9250.47
297_K323_Q0.9210.46
391_L431_V0.9190.46
123_C132_E0.9170.46
133_I436_E0.9150.46
362_L379_V0.9130.46
243_Q269_H0.9110.46
234_A240_T0.9040.45
302_A355_V0.9030.45
94_D102_N0.9010.45
412_F418_L0.9010.45
113_V420_H0.9000.45
303_T331_H0.8990.45
223_C257_K0.8990.45
52_E55_G0.8990.45
200_G223_C0.8980.45
331_H376_T0.8930.44
225_Q378_T0.8900.44
298_I370_D0.8900.44
369_V383_A0.8900.44
225_Q254_F0.8870.44
147_E151_S0.8810.44
67_Q70_F0.8810.44
65_Q68_P0.8790.43
328_I446_N0.8770.43
64_G67_Q0.8760.43
53_L57_V0.8750.43
419_A423_V0.8700.43
412_F415_P0.8690.43
117_W121_H0.8660.42
107_D110_K0.8640.42
271_R396_S0.8620.42
263_Q454_N0.8620.42
234_A238_V0.8610.42
61_I66_I0.8590.42
66_I71_E0.8590.42
201_T224_L0.8580.42
271_R304_Q0.8580.42
304_Q381_L0.8550.42
87_G91_L0.8520.41
61_I65_Q0.8520.41
143_K326_I0.8510.41
48_E65_Q0.8500.41
55_G70_F0.8480.41
414_Q425_V0.8390.40
226_H375_Q0.8360.40
71_E78_V0.8360.40
70_F90_I0.8330.40
53_L61_I0.8330.40
79_K85_V0.8320.40
109_E112_A0.8310.40
234_A237_P0.8300.40
362_L366_L0.8300.40
51_P70_F0.8300.40
48_E57_V0.8260.40
164_D167_F0.8250.39
138_Q160_P0.8250.39
219_A383_A0.8230.39
121_H128_I0.8210.39
86_K96_L0.8150.39
103_Y111_T0.8150.39
76_N79_K0.8140.39
373_V396_S0.8130.39
173_K199_V0.8120.39
376_T396_S0.8100.38
381_L408_G0.8080.38
326_I460_L0.8050.38
47_M55_G0.8050.38
186_D190_E0.8030.38
162_F167_F0.8000.38
381_L384_M0.7980.38
223_C390_V0.7950.37
329_K332_P0.7930.37
372_A375_Q0.7920.37
276_F385_L0.7850.37
244_A265_L0.7850.37
123_C130_K0.7850.37
383_A388_K0.7840.37
52_E58_D0.7810.36
225_Q231_G0.7800.36
166_A169_D0.7790.36
228_A454_N0.7780.36
174_D178_F0.7750.36
71_E98_F0.7750.36
231_G329_K0.7750.36
201_T383_A0.7720.36
70_F423_V0.7720.36
458_A462_A0.7700.36
221_S333_W0.7670.35
228_A231_G0.7650.35
439_R446_N0.7630.35
135_D160_P0.7630.35
224_L359_E0.7590.35
415_P418_L0.7590.35
230_M269_H0.7580.35
226_H270_P0.7570.35
171_L176_P0.7550.35
351_Q354_R0.7520.34
353_C411_M0.7510.34
66_I81_N0.7500.34
327_I354_R0.7480.34
52_E57_V0.7480.34
63_E103_Y0.7470.34
224_L250_E0.7460.34
226_H379_V0.7450.34
54_F57_V0.7450.34
123_C134_A0.7440.34
182_I188_L0.7440.34
305_P376_T0.7430.34
306_F408_G0.7420.34
90_I104_Y0.7410.34
117_W120_P0.7400.34
376_T381_L0.7390.34
76_N107_D0.7390.34
128_I132_E0.7380.34
47_M56_S0.7370.33
89_V101_E0.7370.33
302_A453_T0.7360.33
378_T382_E0.7360.33
124_D127_V0.7360.33
274_Q330_P0.7350.33
53_L58_D0.7300.33
106_F116_R0.7300.33
154_A243_Q0.7280.33
355_V359_E0.7250.33
181_A185_V0.7210.32
227_G378_T0.7200.32
127_V133_I0.7190.32
304_Q377_S0.7160.32
64_G91_L0.7160.32
58_D69_F0.7160.32
247_G378_T0.7150.32
235_F239_F0.7140.32
213_M362_L0.7140.32
227_G331_H0.7140.32
329_K334_E0.7130.32
59_Q65_Q0.7130.32
140_G201_T0.7120.32
373_V391_L0.7110.32
89_V114_L0.7110.32
225_Q307_S0.7110.32
378_T400_S0.7100.32
268_G377_S0.7100.32
124_D133_I0.7080.32
307_S329_K0.7070.32
227_G304_Q0.7030.31
394_K403_Y0.7020.31
65_Q72_E0.7010.31
96_L100_N0.6980.31
326_I441_A0.6970.31
76_N115_A0.6960.31
62_D71_E0.6960.31
127_V130_K0.6950.31
448_P461_K0.6940.31
70_F74_I0.6930.31
53_L59_Q0.6920.31
329_K361_E0.6890.30
333_W392_I0.6890.30
108_P115_A0.6880.30
100_N104_Y0.6880.30
305_P385_L0.6880.30
108_P422_L0.6870.30
116_R122_Y0.6850.30
67_Q72_E0.6850.30
225_Q228_A0.6850.30
223_C363_Y0.6840.30
227_G269_H0.6810.30
76_N95_Y0.6810.30
308_E399_R0.6800.30
436_E439_R0.6770.30
251_A366_L0.6750.30
416_D419_A0.6740.29
331_H374_T0.6720.29
271_R382_E0.6710.29
190_E193_K0.6690.29
188_L191_Q0.6690.29
198_I209_V0.6680.29
394_K407_L0.6670.29
212_L330_P0.6660.29
254_F269_H0.6660.29
306_F409_D0.6650.29
94_D117_W0.6640.29
225_Q232_D0.6610.29
323_Q352_A0.6590.29
148_T155_P0.6590.29
95_Y99_S0.6590.29
280_P364_D0.6580.28
417_E434_K0.6560.28
424_E428_S0.6530.28
231_G382_E0.6520.28
442_F446_N0.6520.28
300_L307_S0.6490.28
119_L122_Y0.6450.28
51_P55_G0.6440.28
198_I221_S0.6420.28
76_N80_T0.6420.28
334_E381_L0.6360.27
175_I178_F0.6340.27
414_Q418_L0.6330.27
64_G102_N0.6330.27
51_P64_G0.6330.27
54_F68_P0.6320.27
227_G382_E0.6320.27
87_G95_Y0.6310.27
396_S400_S0.6300.27
228_A250_E0.6290.27
173_K178_F0.6270.27
126_P130_K0.6270.27
246_F304_Q0.6270.27
435_A442_F0.6260.27
307_S363_Y0.6260.27
61_I122_Y0.6250.27
47_M54_F0.6240.26
62_D76_N0.6230.26
125_L131_R0.6220.26
233_E238_V0.6220.26
198_I213_M0.6220.26
62_D66_I0.6220.26
64_G128_I0.6210.26
218_G270_P0.6210.26
69_F110_K0.6200.26
230_M245_V0.6190.26
225_Q304_Q0.6170.26
86_K90_I0.6160.26
269_H395_S0.6160.26
223_C272_F0.6150.26
55_G69_F0.6140.26
138_Q307_S0.6140.26
223_C250_E0.6140.26
266_V456_L0.6130.26
229_L394_K0.6120.26
62_D67_Q0.6120.26
369_V372_A0.6120.26
299_V324_L0.6110.26
126_P461_K0.6080.26
128_I131_R0.6080.26
200_G305_P0.6070.25
343_Y347_A0.6070.25
176_P182_I0.6050.25
201_T335_I0.6050.25
190_E194_I0.6020.25
439_R442_F0.6020.25
82_D91_L0.6020.25
121_H127_V0.6010.25
273_D450_A0.6010.25
348_K351_Q0.6000.25
48_E55_G0.6000.25
201_T372_A0.6000.25
75_Q78_V0.5990.25
127_V320_D0.5990.25
343_Y346_A0.5970.25
62_D73_R0.5940.25
54_F67_Q0.5940.25
187_A191_Q0.5920.25
272_F362_L0.5910.25
301_I395_S0.5880.24
378_T396_S0.5870.24
234_A239_F0.5860.24
55_G83_E0.5850.24
228_A243_Q0.5850.24
66_I92_H0.5840.24
208_R211_A0.5820.24
363_Y381_L0.5800.24
247_G305_P0.5800.24
84_P152_L0.5780.24
63_E73_R0.5770.24
75_Q414_Q0.5770.24
269_H272_F0.5770.24
324_L357_K0.5770.24
178_F182_I0.5770.24
57_V61_I0.5760.24
201_T231_G0.5750.24
92_H261_P0.5750.24
328_I379_V0.5750.24
144_Q400_S0.5710.24
106_F111_T0.5690.23
175_I181_A0.5670.23
363_Y392_I0.5650.23
95_Y100_N0.5650.23
269_H385_L0.5650.23
227_G249_Y0.5640.23
384_M391_L0.5630.23
224_L376_T0.5610.23
208_R357_K0.5600.23
230_M272_F0.5580.23
221_S225_Q0.5580.23
103_Y106_F0.5580.23
54_F66_I0.5570.23
302_A329_K0.5560.23
48_E63_E0.5540.23
117_W128_I0.5520.23
270_P378_T0.5520.23
391_L421_T0.5520.23
272_F381_L0.5510.22
76_N81_N0.5500.22
56_S59_Q0.5500.22
54_F59_Q0.5500.22
125_L320_D0.5490.22
380_G390_V0.5490.22
305_P331_H0.5480.22
247_G253_W0.5480.22
95_Y102_N0.5470.22
225_Q361_E0.5460.22
135_D144_Q0.5450.22
85_V103_Y0.5440.22
67_Q80_T0.5430.22
384_M392_I0.5420.22
228_A395_S0.5420.22
148_T154_A0.5420.22
375_Q381_L0.5410.22
68_P75_Q0.5400.22
323_Q423_V0.5380.22
77_I98_F0.5380.22
269_H305_P0.5370.22
200_G406_S0.5370.22
383_A386_F0.5370.22
66_I80_T0.5360.22
254_F380_G0.5360.22
77_I113_V0.5350.22
50_E59_Q0.5350.22
361_E364_D0.5350.22
72_E152_L0.5340.22
56_S69_F0.5340.22
271_R388_K0.5340.22
381_L392_I0.5330.22
70_F78_V0.5330.22
453_T458_A0.5310.21
51_P57_V0.5310.21
51_P66_I0.5310.21
357_K379_V0.5310.21
135_D332_P0.5300.21
112_A120_P0.5290.21
209_V212_L0.5290.21
275_I386_F0.5290.21
291_A300_L0.5280.21
94_D455_A0.5260.21
306_F374_T0.5260.21
315_L348_K0.5240.21
255_I261_P0.5230.21
183_D186_D0.5210.21
436_E455_A0.5210.21
275_I385_L0.5190.21
187_A190_E0.5180.21
361_E366_L0.5170.21
76_N88_K0.5160.21
145_Y458_A0.5160.21
68_P89_V0.5150.21
277_N363_Y0.5150.21
419_A450_A0.5150.21
71_E77_I0.5150.21
392_I407_L0.5130.21
196_A381_L0.5130.21
97_R297_K0.5120.21
379_V386_F0.5090.20
47_M58_D0.5090.20
198_I461_K0.5090.20
144_Q363_Y0.5080.20
110_K115_A0.5070.20
246_F378_T0.5070.20
194_I217_K0.5050.20
318_L388_K0.5030.20
270_P381_L0.5030.20
227_G377_S0.5000.20
199_V366_L0.5000.20
57_V69_F0.5000.20
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3l7iA 4 0.7553 100 0.463 Contact Map
1vgvA 2 0.7342 99.8 0.778 Contact Map
3dzcA 2 0.7236 99.8 0.791 Contact Map
4nesA 2 0.7068 99.7 0.795 Contact Map
1v4vA 2 0.7321 99.7 0.799 Contact Map
4hwgA 5 0.7363 99.7 0.809 Contact Map
3ot5A 2 0.7089 99.6 0.81 Contact Map
4fkzA 2 0.7384 99.6 0.816 Contact Map
3okpA 1 0.6983 99.5 0.828 Contact Map
2jjmA 4 0.6709 99.4 0.84 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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