GREMLIN Database
SPSA - Spore coat polysaccharide biosynthesis protein SpsA
UniProt: P39621 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 256 (208)
Sequences: 39373 (33773)
Seq/√Len: 2341.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
166_Y170_E3.0611.00
13_K98_D3.0141.00
14_S44_E2.5681.00
193_R197_R2.3561.00
109_K209_E2.3421.00
63_S83_Q2.3341.00
38_D62_Q2.2391.00
5_S90_G2.2231.00
83_Q87_M2.2181.00
103_M213_L2.1941.00
92_Y114_L2.0971.00
10_S45_T2.0811.00
46_L62_Q2.0721.00
28_T108_L2.0511.00
33_E59_R2.0411.00
37_M84_A1.9391.00
18_A22_S1.9281.00
8_M34_L1.9251.00
46_L50_R1.9171.00
63_S75_T1.9001.00
75_T79_A1.8891.00
7_I84_A1.8761.00
120_K205_Y1.8511.00
121_A202_Y1.8201.00
19_K215_Y1.8081.00
86_E178_S1.7771.00
18_A52_F1.7501.00
168_V202_Y1.7241.00
12_N42_N1.7161.00
44_E47_N1.7131.00
81_I97_T1.6941.00
61_Y87_M1.6791.00
113_E207_L1.6771.00
5_S33_E1.6761.00
19_K101_I1.6551.00
102_Y106_R1.6521.00
94_T163_M1.6451.00
113_E205_Y1.6441.00
14_S48_V1.6381.00
4_V111_V1.6361.00
128_K213_L1.6311.00
77_Y81_I1.6061.00
29_F111_V1.5931.00
105_D109_K1.5801.00
110_M163_M1.5771.00
50_R62_Q1.5751.00
121_A165_R1.5521.00
38_D41_S1.5391.00
106_R110_M1.5371.00
59_R87_M1.5191.00
172_V177_G1.5161.00
8_M20_S1.4961.00
83_Q86_E1.4851.00
101_I213_L1.4791.00
36_I49_I1.4761.00
92_Y163_M1.4431.00
3_K91_E1.4371.00
18_A48_V1.4301.00
22_S26_S1.4191.00
63_S79_A1.4071.00
96_A102_Y1.4031.00
22_S52_F1.4031.00
10_S36_I1.4011.00
18_A51_P1.3961.00
122_V204_F1.3951.00
114_L163_M1.3841.00
33_E90_G1.3831.00
94_T106_R1.3821.00
11_Y74_K1.3711.00
46_L60_F1.3601.00
94_T107_L1.3491.00
109_K112_R1.3451.00
61_Y83_Q1.3421.00
78_A190_G1.3291.00
29_F32_F1.3201.00
108_L112_R1.3021.00
167_S171_K1.2971.00
4_V107_L1.2791.00
81_I95_Y1.2551.00
93_I166_Y1.2461.00
5_S88_A1.2401.00
85_I169_L1.2271.00
162_V195_F1.2261.00
23_S102_Y1.2241.00
171_K202_Y1.2191.00
10_S13_K1.2161.00
50_R53_L1.1671.00
21_I34_L1.1591.00
3_K31_D1.1571.00
12_N41_S1.1531.00
10_S98_D1.1481.00
48_V52_F1.1471.00
114_L123_I1.1441.00
63_S80_L1.1281.00
91_E165_R1.1271.00
13_K16_Y1.1221.00
110_M125_S1.1211.00
77_Y190_G1.1171.00
85_I95_Y1.1101.00
130_Y213_L1.1091.00
101_I215_Y1.1021.00
39_D74_K1.1011.00
88_A166_Y1.1001.00
126_A206_P1.0981.00
168_V198_V1.0951.00
113_E116_T1.0611.00
167_S170_E1.0561.00
111_V115_D1.0551.00
20_S96_A1.0551.00
25_L55_D1.0541.00
112_R116_T1.0521.00
33_E89_E1.0441.00
105_D108_L1.0361.00
4_V163_M1.0341.00
37_M80_L1.0321.00
26_S104_P1.0221.00
38_D46_L1.0191.00
77_Y221_I0.9921.00
5_S35_F0.9881.00
94_T102_Y0.9761.00
165_R168_V0.9731.00
128_K210_E0.9601.00
119_E203_P0.9591.00
51_P54_N0.9561.00
34_L58_V0.9511.00
101_I130_Y0.9471.00
48_V51_P0.9411.00
22_S25_L0.9401.00
47_N50_R0.9401.00
9_T80_L0.9361.00
109_K207_L0.9341.00
39_D63_S0.9321.00
3_K89_E0.9301.00
23_S101_I0.9221.00
110_M123_I0.9201.00
28_T105_D0.9161.00
127_S160_C0.9151.00
121_A203_P0.9151.00
169_L194_F0.9141.00
3_K90_G0.9111.00
59_R89_E0.9101.00
49_I60_F0.9071.00
25_L52_F0.9021.00
113_E123_I0.9021.00
103_M128_K0.8991.00
24_I96_A0.8971.00
122_V198_V0.8971.00
171_K200_H0.8931.00
106_R211_L0.8911.00
106_R125_S0.8911.00
85_I93_I0.8881.00
110_M114_L0.8531.00
25_L57_R0.8491.00
6_V24_I0.8491.00
81_I190_G0.8451.00
172_V178_S0.8431.00
92_Y111_V0.8381.00
75_T80_L0.8351.00
109_K113_E0.8261.00
44_E48_V0.8201.00
79_A83_Q0.8191.00
78_A82_N0.8161.00
10_S17_V0.8011.00
25_L58_V0.8011.00
39_D80_L0.7991.00
24_I34_L0.7881.00
77_Y159_H0.7881.00
217_T220_S0.7881.00
21_I52_F0.7861.00
6_V94_T0.7811.00
16_Y215_Y0.7681.00
3_K115_D0.7651.00
10_S49_I0.7541.00
47_N51_P0.7541.00
77_Y97_T0.7481.00
39_D76_R0.7471.00
63_S73_E0.7441.00
136_R139_V0.7431.00
20_S23_S0.7411.00
53_L60_F0.7391.00
37_M83_Q0.7331.00
39_D75_T0.7331.00
121_A168_V0.7321.00
21_I36_I0.7291.00
29_F108_L0.7281.00
186_F189_I0.7241.00
13_K217_T0.7231.00
32_F107_L0.7191.00
79_A184_P0.7171.00
19_K22_S0.7101.00
130_Y215_Y0.7091.00
125_S211_L0.7081.00
24_I102_Y0.7081.00
159_H162_V0.7071.00
129_T216_I0.7001.00
184_P187_Y0.6991.00
82_N178_S0.6991.00
220_S223_F0.6971.00
168_V172_V0.6941.00
6_V107_L0.6921.00
77_Y80_L0.6881.00
28_T104_P0.6821.00
40_N63_S0.6811.00
164_H195_F0.6761.00
124_Y206_P0.6761.00
172_V198_V0.6701.00
53_L58_V0.6661.00
9_T98_D0.6641.00
160_C212_D0.6631.00
22_S54_N0.6621.00
172_V200_H0.6621.00
95_Y179_Y0.6601.00
85_I88_A0.6601.00
52_F55_D0.6601.00
35_F84_A0.6581.00
14_S18_A0.6581.00
80_L83_Q0.6581.00
113_E120_K0.6581.00
103_M211_L0.6571.00
4_V94_T0.6541.00
8_M24_I0.6521.00
194_F198_V0.6491.00
126_A208_D0.6491.00
103_M106_R0.6441.00
168_V171_K0.6391.00
159_H179_Y0.6371.00
29_F107_L0.6291.00
88_A93_I0.6281.00
111_V163_M0.6281.00
35_F87_M0.6281.00
171_K201_F0.6281.00
114_L120_K0.6241.00
17_V98_D0.6241.00
21_I25_L0.6181.00
63_S74_K0.6181.00
50_R60_F0.6151.00
59_R62_Q0.6151.00
91_E167_S0.6121.00
46_L49_I0.6101.00
11_Y39_D0.6091.00
79_A185_A0.6071.00
17_V48_V0.6041.00
164_H194_F0.6041.00
125_S161_S0.6031.00
75_T83_Q0.6021.00
16_Y217_T0.6011.00
20_S99_D0.5991.00
82_N86_E0.5981.00
49_I53_L0.5961.00
112_R115_D0.5931.00
55_D58_V0.5921.00
225_L229_E0.5891.00
178_S194_F0.5881.00
106_R161_S0.5841.00
20_S24_I0.5841.00
94_T161_S0.5821.00
9_T81_I0.5801.00
207_L210_E0.5801.00
164_H169_L0.5751.00
37_M63_S0.5751.00
40_N74_K0.5731.00
74_K80_L0.5721.00
14_S47_N0.5721.00
189_I192_A0.5711.00
100_N214_N0.5711.00
85_I178_S0.5701.00
151_W155_C0.5701.00
9_T39_D0.5681.00
6_V32_F0.5681.00
3_K32_F0.5621.00
114_L165_R0.5591.00
97_T190_G0.5561.00
124_Y157_I0.5551.00
192_A196_W0.5531.00
92_Y165_R0.5521.00
152_N155_C0.5511.00
109_K211_L0.5511.00
14_S17_V0.5501.00
169_L178_S0.5491.00
27_Q108_L0.5441.00
151_W154_P0.5441.00
18_A54_N0.5421.00
35_F88_A0.5411.00
25_L54_N0.5411.00
17_V49_I0.5401.00
35_F59_R0.5401.00
157_I195_F0.5391.00
226_F229_E0.5391.00
28_T32_F0.5381.00
8_M21_I0.5361.00
170_E178_S0.5351.00
102_Y107_L0.5321.00
143_V147_A0.5321.00
149_V152_N0.5301.00
9_T77_Y0.5251.00
5_S89_E0.5241.00
10_S38_D0.5211.00
113_E118_P0.5191.00
118_P205_Y0.5171.00
142_T145_P0.5161.00
145_P149_V0.5131.00
100_N160_C0.5101.00
216_I223_F0.5091.00
7_I37_M0.5081.00
3_K29_F0.5081.00
210_E213_L0.5071.00
160_C214_N0.5051.00
199_N204_F0.5041.00
144_R147_A0.5031.00
121_A167_S0.5021.00
121_A124_Y0.5011.00
96_A100_N0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1qg8A 1 0.9102 100 0.215 Contact Map
3bcvA 2 0.6914 100 0.297 Contact Map
3l7iA 4 0 100 0.303 Contact Map
2z86A 2 0.918 100 0.309 Contact Map
2d7iA 1 0.9336 100 0.326 Contact Map
5ajoA 1 0.9258 100 0.343 Contact Map
1xhbA 1 0.9023 100 0.344 Contact Map
4fixA 3 0.9219 100 0.386 Contact Map
3ckjA 2 0.8984 100 0.412 Contact Map
4p02A 1 0.9102 100 0.415 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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