GREMLIN Database
NFRA1 - FMN reductase (NADPH)
UniProt: P39605 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 249 (238)
Sequences: 1508 (1111)
Seq/√Len: 72.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
52_S185_N4.1871.00
122_S132_I3.4881.00
80_C155_V3.4101.00
60_L145_V3.3551.00
8_I123_I3.2321.00
23_T26_E3.1671.00
56_K185_N3.0131.00
58_R71_E2.9351.00
81_A110_S2.9181.00
150_Q186_T2.8611.00
141_E144_K2.6171.00
20_Q165_H2.6151.00
238_N242_K2.5861.00
78_V149_L2.4771.00
38_S132_I2.4521.00
30_L121_M2.3171.00
143_D243_E2.2761.00
122_S161_L2.2361.00
50_G75_H2.2361.00
3_N6_E2.2151.00
50_G182_Y2.1251.00
26_E165_H2.0981.00
54_P58_R2.0911.00
138_I145_V2.0851.00
17_F162_A2.0451.00
79_F121_M2.0341.00
198_M222_S2.0061.00
52_S184_E2.0061.00
202_D222_S2.0041.00
24_A52_S1.9971.00
130_L165_H1.9961.00
18_T167_A1.9831.00
14_I170_S1.9501.00
56_K186_T1.9481.00
118_A134_Y1.9101.00
28_D178_K1.8561.00
146_T244_K1.8281.00
144_K147_E1.8091.00
34_A121_M1.7571.00
146_T240_Y1.7421.00
29_T128_M1.7341.00
77_F121_M1.7051.00
59_E62_V1.6951.00
55_E58_R1.6921.00
48_I77_F1.6801.00
133_C162_A1.6651.00
9_L29_T1.6531.00
63_L141_E1.6441.00
16_S167_A1.6400.99
22_L74_G1.6400.99
26_E30_L1.6300.99
53_D56_K1.5940.99
37_A116_L1.5860.99
31_V182_Y1.5700.99
62_V66_N1.5660.99
63_L144_K1.5630.99
84_Y189_V1.5490.99
18_T165_H1.5470.99
48_I79_F1.5320.99
135_I162_A1.5280.99
143_D147_E1.5240.99
108_M111_L1.5100.99
203_K207_D1.4780.99
76_F160_G1.4270.99
59_E144_K1.3850.98
26_E128_M1.3770.98
28_D32_K1.3740.98
76_F135_I1.3610.98
32_K35_Q1.3580.98
140_N234_R1.3250.98
59_E148_V1.3190.98
24_A28_D1.3020.97
61_S71_E1.2810.97
53_D185_N1.2730.97
26_E130_L1.2680.97
179_Q197_T1.2670.97
38_S122_S1.2510.97
208_Y212_R1.2500.97
132_I163_V1.2130.96
27_I77_F1.2110.96
46_Y117_A1.2070.96
22_L25_E1.2070.96
207_D210_R1.2000.96
56_K59_E1.1990.96
153_D244_K1.1910.96
77_F163_V1.1860.95
56_K148_V1.1740.95
105_E139_R1.1490.95
27_I31_V1.1470.95
35_Q181_V1.1410.94
219_E224_Q1.1380.94
87_Q154_H1.1370.94
88_Q91_E1.1360.94
93_K202_D1.1350.94
6_E10_N1.1230.94
76_F162_A1.1230.94
202_D220_T1.1050.93
125_A132_I1.1040.93
118_A159_F1.0920.93
122_S134_Y1.0800.93
113_D136_G1.0770.93
84_Y88_Q1.0690.92
206_S220_T1.0680.92
107_F110_S1.0650.92
65_G137_G1.0600.92
30_L125_A1.0520.92
69_Y168_N1.0500.92
11_H120_N1.0490.92
49_I80_C1.0330.91
239_D243_E1.0320.91
32_K178_K1.0290.91
55_E59_E1.0270.91
36_A172_K1.0240.91
180_A197_T1.0230.90
188_N191_T1.0230.90
23_T165_H1.0170.90
48_I182_Y1.0080.90
29_T33_S1.0020.90
54_P186_T0.9990.89
5_I124_A0.9910.89
217_R224_Q0.9740.88
236_Y239_D0.9740.88
92_E199_N0.9730.88
207_D211_E0.9720.88
4_T123_I0.9710.88
117_A121_M0.9640.88
24_A184_E0.9600.87
107_F241_V0.9590.87
64_A137_G0.9540.87
69_Y135_I0.9490.87
132_I161_L0.9450.87
48_I117_A0.9420.86
87_Q91_E0.9380.86
47_S183_H0.9360.86
9_L32_K0.9150.85
108_M112_I0.9120.85
50_G184_E0.9010.84
40_S105_E0.8910.83
12_R170_S0.8900.83
112_I159_F0.8870.83
142_L240_Y0.8870.83
9_L33_S0.8850.83
11_H126_E0.8840.83
54_P57_K0.8780.82
81_A158_L0.8770.82
61_S64_A0.8700.82
68_P72_K0.8680.82
31_V50_G0.8640.81
220_T223_D0.8580.81
68_P71_E0.8560.81
28_D179_Q0.8490.80
151_T187_Y0.8460.80
8_I33_S0.8450.80
78_V138_I0.8450.80
4_T7_T0.8450.80
8_I124_A0.8430.80
115_A159_F0.8430.80
196_H199_N0.8380.79
11_H132_I0.8370.79
16_S168_N0.8340.79
73_N162_A0.8310.79
4_T127_S0.8270.78
46_Y181_V0.8250.78
232_K235_T0.8220.78
171_G213_T0.8200.78
14_I168_N0.8070.77
119_Q134_Y0.8050.76
236_Y240_Y0.8050.76
111_L156_L0.8010.76
38_S134_Y0.8000.76
22_L26_E0.7970.76
16_S72_K0.7950.76
63_L148_V0.7940.75
5_I127_S0.7920.75
134_Y159_F0.7880.75
5_I29_T0.7870.75
202_D212_R0.7860.75
34_A117_A0.7830.74
33_S123_I0.7800.74
121_M161_L0.7730.73
57_K162_A0.7720.73
138_I157_P0.7720.73
83_L110_S0.7700.73
56_K150_Q0.7670.73
79_F114_A0.7660.73
210_R218_E0.7650.73
25_E28_D0.7640.73
26_E121_M0.7620.72
151_T155_V0.7610.72
21_L74_G0.7560.72
167_A170_S0.7550.72
6_E9_L0.7540.72
43_V106_M0.7470.71
213_T216_K0.7440.71
75_H182_Y0.7430.71
22_L163_V0.7420.70
84_Y155_V0.7390.70
52_S75_H0.7360.70
75_H163_V0.7360.70
22_L28_D0.7340.70
86_H225_I0.7320.69
239_D242_K0.7290.69
58_R61_S0.7220.68
87_Q153_D0.7040.66
112_I115_A0.7010.66
203_K211_E0.6980.66
131_G166_P0.6980.66
38_S161_L0.6920.65
5_I33_S0.6920.65
180_A198_M0.6890.65
125_A163_V0.6840.64
124_A127_S0.6840.64
31_V178_K0.6820.64
156_L240_Y0.6780.63
105_E234_R0.6760.63
17_F69_Y0.6750.63
91_E94_G0.6750.63
5_I128_M0.6720.63
45_A109_V0.6710.63
67_Q70_V0.6710.63
200_T203_K0.6630.62
77_F161_L0.6600.61
112_I134_Y0.6590.61
76_F137_G0.6500.60
146_T153_D0.6500.60
93_K223_D0.6490.60
42_Y205_I0.6490.60
200_T204_T0.6460.60
46_Y115_A0.6440.59
51_V60_L0.6420.59
139_R221_W0.6410.59
38_S159_F0.6400.59
217_R222_S0.6370.59
51_V149_L0.6360.59
74_G130_L0.6350.58
7_T10_N0.6330.58
216_K219_E0.6280.58
115_A221_W0.6270.57
57_K75_H0.6250.57
40_S140_N0.6230.57
111_L158_L0.6220.57
110_S156_L0.6200.57
178_K182_Y0.6190.56
209_Y224_Q0.6180.56
142_L157_P0.6180.56
6_E32_K0.6180.56
57_K73_N0.6170.56
173_K209_Y0.6160.56
205_I209_Y0.6140.56
229_M233_P0.6140.56
11_H38_S0.6110.56
210_R227_N0.6100.55
50_G77_F0.6090.55
150_Q179_Q0.6060.55
223_D227_N0.6000.54
153_D245_G0.5970.54
108_M237_L0.5960.54
19_D72_K0.5960.54
170_S173_K0.5950.54
183_H188_N0.5910.53
48_I121_M0.5910.53
130_L163_V0.5900.53
206_S218_E0.5890.53
120_N123_I0.5890.53
69_Y133_C0.5870.53
64_A70_V0.5850.52
88_Q189_V0.5850.52
70_V85_R0.5840.52
12_R169_L0.5810.52
51_V78_V0.5810.52
115_A119_Q0.5760.51
40_S136_G0.5760.51
94_G97_I0.5730.51
33_S124_A0.5730.51
50_G57_K0.5700.50
235_T239_D0.5700.50
197_T200_T0.5700.50
86_H142_L0.5640.50
30_L163_V0.5640.50
212_R215_G0.5620.50
73_N76_F0.5620.50
215_G218_E0.5610.49
171_G211_E0.5610.49
31_V37_A0.5600.49
144_K148_V0.5600.49
40_S109_V0.5590.49
34_A120_N0.5580.49
211_E243_E0.5580.49
116_L119_Q0.5570.49
26_E29_T0.5560.49
13_S135_I0.5560.49
27_I182_Y0.5560.49
42_Y201_Y0.5550.49
225_I229_M0.5520.48
54_P203_K0.5510.48
40_S160_G0.5510.48
22_L29_T0.5500.48
10_N172_K0.5500.48
64_A67_Q0.5500.48
32_K172_K0.5500.48
84_Y188_N0.5490.48
46_Y114_A0.5490.48
60_L138_I0.5490.48
22_L128_M0.5460.48
63_L145_V0.5460.48
63_L137_G0.5420.47
150_Q153_D0.5410.47
89_L92_E0.5410.47
57_K60_L0.5400.47
74_G165_H0.5350.46
10_N169_L0.5340.46
150_Q189_V0.5340.46
5_I9_L0.5340.46
142_L229_M0.5320.46
92_E202_D0.5310.46
236_Y243_E0.5310.46
93_K203_K0.5300.46
38_S126_E0.5300.46
81_A155_V0.5280.45
47_S85_R0.5270.45
101_L111_L0.5270.45
207_D218_E0.5260.45
191_T200_T0.5260.45
6_E81_A0.5240.45
204_T227_N0.5230.45
111_L115_A0.5230.45
87_Q98_S0.5220.45
61_S66_N0.5210.44
11_H123_I0.5200.44
4_T8_I0.5200.44
4_T9_L0.5200.44
84_Y154_H0.5200.44
41_S134_Y0.5200.44
43_V228_F0.5180.44
40_S69_Y0.5180.44
221_W229_M0.5150.44
147_E242_K0.5150.44
38_S173_K0.5130.44
225_I231_Q0.5120.43
43_V229_M0.5100.43
36_A173_K0.5100.43
93_K176_L0.5100.43
40_S173_K0.5090.43
82_D187_Y0.5080.43
79_F118_A0.5080.43
218_E223_D0.5050.43
209_Y212_R0.5040.42
77_F182_Y0.5030.42
204_T207_D0.5030.42
8_I38_S0.5020.42
85_R151_T0.5020.42
130_L166_P0.5010.42
99_E117_A0.5010.42
16_S169_L0.5010.42
15_R136_G0.5000.42
199_N203_K0.5000.42
180_A187_Y0.5000.42
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3n2sA 2 0.996 100 0.222 Contact Map
1zchA 2 0.9839 100 0.227 Contact Map
1f5vA 2 0.9639 100 0.239 Contact Map
1bkjA 2 0.9237 100 0.239 Contact Map
3eofA 2 0.9398 100 0.309 Contact Map
3gagA 4 0.739 100 0.466 Contact Map
3bemA 2 0.7349 100 0.467 Contact Map
1noxA 2 0.7349 100 0.47 Contact Map
3gbhA 3 0.7349 100 0.472 Contact Map
3ge6A 2 0.7349 100 0.473 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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