GREMLIN Database
THIE - Thiamine-phosphate synthase
UniProt: P39594 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 222 (197)
Sequences: 5319 (3962)
Seq/√Len: 282.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
145_T195_I2.9211.00
59_I87_N2.7561.00
89_K112_M2.5601.00
205_E209_R2.4731.00
62_A88_L2.3021.00
29_V64_K2.2901.00
66_Q70_R2.2621.00
51_D57_A2.1791.00
36_L74_V2.1361.00
178_I210_K2.1271.00
109_I112_M2.1061.00
33_Q37_K2.0941.00
126_Q130_D2.0761.00
181_A192_V2.0591.00
203_D206_S2.0501.00
52_A81_D2.0461.00
33_Q71_E2.0231.00
109_I114_L1.9861.00
128_E167_S1.9781.00
37_K205_E1.9051.00
141_Y180_N1.8951.00
97_Q123_E1.8501.00
36_L68_A1.8491.00
162_R190_D1.8371.00
95_I105_V1.8031.00
70_R91_D1.8001.00
104_E107_A1.7951.00
182_A214_E1.7891.00
162_R170_I1.7841.00
141_Y183_P1.7381.00
17_F199_S1.7101.00
32_V65_A1.6771.00
140_I158_I1.6721.00
69_C76_F1.6601.00
59_I63_E1.6581.00
206_S210_K1.6481.00
17_F198_I1.6001.00
36_L72_A1.5841.00
119_H123_E1.5721.00
139_P152_V1.5461.00
17_F40_A1.5341.00
38_G205_E1.5301.00
197_A211_F1.5131.00
138_G173_I1.4961.00
115_G134_Y1.4451.00
121_M125_K1.4401.00
34_K205_E1.4011.00
38_G208_A1.3941.00
128_E168_I1.3901.00
106_R110_G1.3821.00
59_I88_L1.3771.00
47_E50_G1.3711.00
125_K128_E1.3461.00
177_T180_N1.3441.00
102_A116_V1.3421.00
66_Q91_D1.3401.00
58_R81_D1.3351.00
201_A207_A1.3281.00
37_K72_A1.3241.00
66_Q76_F1.3001.00
66_Q69_C1.2991.00
93_I114_L1.2951.00
178_I211_F1.2941.00
36_L69_C1.2881.00
101_N104_E1.2851.00
96_G99_D1.2781.00
116_V132_A1.2691.00
82_V93_I1.2631.00
103_K130_D1.2611.00
42_L77_I1.2551.00
59_I84_L1.2271.00
41_T212_R1.2091.00
40_A43_Y1.2031.00
45_F76_F1.1991.00
60_K64_K1.1841.00
33_Q68_A1.1821.00
44_Q79_N1.1821.00
45_F58_R1.1781.00
183_P186_Q1.1771.00
124_V161_V1.1761.00
207_A210_K1.1741.00
29_V68_A1.1651.00
19_M43_Y1.1631.00
64_K67_A1.1601.00
153_Q157_L1.1551.00
124_V166_I1.1391.00
181_A214_E1.1251.00
45_F61_F1.1151.00
21_S47_E1.1151.00
185_I215_I1.1121.00
60_K63_E1.1031.00
120_T123_E1.0911.00
33_Q72_A1.0911.00
206_S209_R1.0901.00
57_A60_K1.0811.00
142_P152_V1.0801.00
137_L170_I1.0631.00
20_G23_N1.0571.00
198_I208_A1.0561.00
157_L160_A1.0491.00
144_E196_S1.0451.00
86_L108_A1.0381.00
121_M160_A1.0381.00
209_R213_E1.0331.00
81_D84_L1.0311.00
125_K129_E1.0291.00
66_Q88_L1.0291.00
171_V191_G1.0221.00
32_V43_Y1.0211.00
197_A207_A1.0161.00
121_M161_V1.0111.00
95_I116_V1.0081.00
126_Q129_E1.0071.00
156_S160_A0.9901.00
102_A132_A0.9731.00
47_E61_F0.9721.00
41_T74_V0.9671.00
140_I172_G0.9601.00
27_D31_V0.9571.00
105_V109_I0.9571.00
158_I162_R0.9551.00
154_G157_L0.9501.00
159_E187_A0.9481.00
30_T34_K0.9471.00
56_E60_K0.9361.00
68_A72_A0.9321.00
118_A124_V0.9291.00
28_P32_V0.9291.00
26_A30_T0.9261.00
156_S159_E0.9241.00
97_Q116_V0.9201.00
94_H134_Y0.9121.00
158_I172_G0.9081.00
67_A71_E0.9071.00
45_F62_A0.9051.00
210_K214_E0.9031.00
184_V189_A0.8921.00
208_A212_R0.8921.00
18_I173_I0.8891.00
18_I195_I0.8891.00
57_A61_F0.8851.00
125_K165_G0.8821.00
122_S125_K0.8761.00
51_D56_E0.8741.00
16_Y44_Q0.8731.00
67_A70_R0.8711.00
155_V187_A0.8651.00
62_A78_V0.8441.00
196_S200_Q0.8421.00
137_L161_V0.8361.00
50_G58_R0.8361.00
159_E164_Q0.8351.00
77_I92_G0.8341.00
181_A185_I0.8321.00
52_A100_A0.8261.00
177_T196_S0.8261.00
78_V90_A0.8221.00
31_V199_S0.8221.00
50_G57_A0.8201.00
140_I155_V0.8181.00
194_M211_F0.8150.99
102_A114_L0.8140.99
65_A76_F0.8130.99
40_A208_A0.8060.99
19_M32_V0.8020.99
182_A186_Q0.7990.99
176_I184_V0.7970.99
121_M165_G0.7970.99
91_D113_I0.7900.99
124_V135_V0.7900.99
177_T197_A0.7900.99
83_E87_N0.7860.99
40_A198_I0.7830.99
155_V184_V0.7810.99
16_Y171_V0.7800.99
100_A108_A0.7690.99
17_F35_A0.7680.99
137_L157_L0.7670.99
52_A87_N0.7660.99
15_V215_I0.7590.99
116_V130_D0.7580.99
128_E131_G0.7580.99
77_I113_I0.7520.99
194_M198_I0.7500.99
80_D96_G0.7500.99
84_L88_L0.7450.99
58_R84_L0.7450.99
198_I207_A0.7430.99
118_A127_A0.7420.99
155_V183_P0.7400.99
35_A198_I0.7380.99
58_R79_N0.7330.99
83_E86_L0.7320.99
135_V170_I0.7270.99
122_S126_Q0.7250.99
14_S212_R0.7170.99
62_A90_A0.7150.99
178_I207_A0.7060.99
176_I180_N0.7010.98
101_N126_Q0.6970.98
80_D99_D0.6970.98
63_E88_L0.6960.98
95_I114_L0.6960.98
21_S49_G0.6950.98
170_I190_D0.6890.98
48_K146_K0.6850.98
30_T33_Q0.6820.98
180_N184_V0.6820.98
35_A38_G0.6800.98
69_C72_A0.6780.98
93_I105_V0.6680.98
29_V61_F0.6670.98
52_A83_E0.6660.98
210_K213_E0.6590.98
27_D30_T0.6570.98
36_L43_Y0.6560.98
18_I193_S0.6550.98
34_K37_K0.6540.98
106_R114_L0.6540.98
128_E166_I0.6530.98
22_N49_G0.6490.97
106_R133_D0.6460.97
136_G173_I0.6450.97
15_V212_R0.6410.97
33_Q64_K0.6370.97
24_T31_V0.6350.97
29_V65_A0.6260.97
125_K166_I0.6190.97
34_K38_G0.6160.97
137_L153_Q0.6150.97
172_G189_A0.6100.96
19_M28_P0.6080.96
204_P207_A0.6070.96
63_E66_Q0.6060.96
44_Q136_G0.6040.96
76_F91_D0.5990.96
100_A104_E0.5970.96
171_V190_D0.5960.96
118_A123_E0.5960.96
21_S61_F0.5920.96
17_F43_Y0.5920.96
143_T196_S0.5880.96
23_N200_Q0.5880.96
207_A211_F0.5870.95
41_T191_G0.5850.95
96_G146_K0.5830.95
41_T75_P0.5810.95
113_I134_Y0.5810.95
87_N104_E0.5800.95
162_R187_A0.5790.95
131_G168_I0.5780.95
178_I181_A0.5770.95
148_D151_A0.5750.95
192_V215_I0.5730.95
48_K96_G0.5690.95
20_G47_E0.5680.95
158_I189_A0.5680.95
49_G147_K0.5620.94
69_C74_V0.5600.94
102_A130_D0.5590.94
160_A165_G0.5590.94
34_K203_D0.5560.94
84_L87_N0.5560.94
66_Q90_A0.5530.94
46_R146_K0.5520.94
14_S41_T0.5520.94
140_I184_V0.5510.94
48_K80_D0.5510.94
42_L75_P0.5490.94
139_P151_A0.5480.94
43_Y69_C0.5480.94
26_A31_V0.5460.93
77_I112_M0.5460.93
34_K204_P0.5460.93
140_I154_G0.5440.93
47_E58_R0.5400.93
66_Q89_K0.5400.93
160_A164_Q0.5360.93
82_V105_V0.5310.92
45_F65_A0.5300.92
48_K79_N0.5260.92
28_P47_E0.5250.92
45_F78_V0.5250.92
127_A131_G0.5240.92
103_K107_A0.5240.92
21_S24_T0.5200.92
158_I187_A0.5190.92
148_D152_V0.5160.91
143_T154_G0.5160.91
97_Q119_H0.5130.91
135_V168_I0.5080.91
16_Y42_L0.5070.91
139_P149_T0.5060.91
29_V32_V0.5050.90
95_I102_A0.5030.90
25_K200_Q0.5000.90
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3nl6A 4 0.9505 100 0.325 Contact Map
3o63A 2 0.9189 100 0.421 Contact Map
2tpsA 1 0.9865 100 0.435 Contact Map
1twdA 2 0.8829 100 0.442 Contact Map
3ceuA 1 0.8468 100 0.447 Contact Map
1yadA 2 0.8694 100 0.456 Contact Map
2yw3A 4 0.8784 100 0.469 Contact Map
1xi3A 2 0.9099 100 0.472 Contact Map
4wuiA 1 0.8694 100 0.478 Contact Map
2bdqA 2 0.7883 100 0.515 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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