GREMLIN Database
PLY - Pectate lyase
UniProt: P39116 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 420 (355)
Sequences: 618 (439)
Seq/√Len: 23.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
168_I175_I4.0511.00
410_V414_A3.9651.00
220_C261_N3.9471.00
71_N75_K3.8771.00
54_Y65_A3.6511.00
262_Y295_K3.4931.00
91_L138_Q3.3361.00
402_A406_V3.3121.00
171_D213_T3.1631.00
406_V410_V3.1111.00
254_N286_K2.7460.99
221_T416_A2.7360.99
209_I212_G2.7210.99
408_S412_N2.6870.99
246_Q258_M2.6720.99
258_M261_N2.6340.99
59_R88_D2.6120.99
162_V182_D2.5030.98
180_F222_F2.4980.98
290_H312_Y2.4620.98
87_V137_N2.4470.98
217_I220_C2.4010.98
59_R63_V2.2980.97
363_G366_L2.2970.97
249_A258_M2.2690.97
173_V215_I2.2320.97
179_E223_N2.2270.97
53_V76_I2.1930.96
33_D290_H2.1920.96
304_V339_I2.1900.96
179_E221_T2.1730.96
303_R334_G2.1150.95
207_I222_F2.1130.95
67_G144_V2.1050.95
177_N417_G2.1030.95
274_S320_T2.0880.95
46_S53_V2.0600.95
255_Y286_K2.0500.95
247_T263_Y2.0350.94
345_V407_K2.0190.94
168_I209_I2.0160.94
314_N344_N2.0130.94
213_T255_Y1.9930.94
343_N370_G1.9810.93
221_T295_K1.9500.93
45_G48_A1.9240.92
288_T312_Y1.9230.92
260_Y291_H1.9010.92
117_T120_K1.8870.92
401_D409_N1.8720.91
168_I173_V1.8550.91
389_S392_W1.8440.91
270_S294_Y1.8310.90
397_H400_I1.8240.90
168_I215_I1.8010.90
333_I339_I1.7990.90
128_E131_R1.7930.89
214_H255_Y1.7840.89
42_T214_H1.7780.89
48_A53_V1.7700.89
297_I301_A1.7150.87
382_A403_S1.7020.87
73_T147_P1.7010.87
315_Y347_D1.6870.86
221_T262_Y1.6700.86
63_V67_G1.6530.85
403_S406_V1.6340.84
27_Q153_V1.6290.84
224_D269_S1.6230.84
175_I178_I1.6150.84
222_F240_Y1.6040.83
47_K53_V1.5990.83
331_W341_A1.5980.83
370_G403_S1.5860.83
356_T359_V1.5640.82
95_G98_D1.5580.81
310_H400_I1.5500.81
210_N251_N1.5480.81
222_F261_N1.5330.80
85_M164_G1.5320.80
146_I166_F1.5030.79
63_V144_V1.4960.79
180_F211_G1.4950.79
86_N162_V1.4920.78
181_Q223_N1.4900.78
396_L399_S1.4870.78
310_H397_H1.4810.78
406_V411_I1.4720.78
125_G129_E1.4560.77
58_N65_A1.4510.76
36_G43_T1.4370.76
95_G134_S1.4290.75
62_L66_L1.4120.74
171_D214_H1.4080.74
189_Q269_S1.4060.74
371_T377_Q1.3800.73
248_D305_R1.3610.72
368_D371_T1.3480.71
66_L77_I1.3380.70
27_Q151_T1.3010.68
184_Y204_Y1.2970.68
401_D406_V1.2940.68
105_D108_K1.2940.68
170_S345_V1.2910.68
125_G130_A1.2880.67
282_D336_S1.2780.67
58_N83_I1.2770.67
161_V418_K1.2760.67
175_I217_I1.2750.67
155_S158_N1.2710.66
408_S411_I1.2690.66
169_K210_N1.2570.65
152_I371_T1.2490.65
18_G173_V1.2450.65
222_F263_Y1.2430.65
31_S41_G1.2390.64
184_Y206_N1.2340.64
209_I215_I1.2240.63
154_G314_N1.2220.63
145_D167_Q1.2220.63
289_L311_V1.2180.63
78_Y155_S1.2100.62
386_L390_V1.1990.62
114_D118_W1.1980.62
21_A24_L1.1970.62
58_N77_I1.1970.62
66_L71_N1.1940.61
288_T414_A1.1920.61
410_V415_G1.1810.60
361_S367_Y1.1730.60
147_P173_V1.1660.59
56_V62_L1.1660.59
209_I256_I1.1650.59
370_G376_T1.1640.59
164_G342_Q1.1610.59
304_V309_V1.1570.59
64_S72_T1.1550.59
392_W395_S1.1550.59
311_V331_W1.1520.58
80_K155_S1.1250.57
259_S410_V1.1200.56
387_S391_G1.1140.56
293_R317_E1.1140.56
407_K411_I1.1130.56
37_A42_T1.1100.56
389_S393_T1.1060.55
168_I207_I1.1050.55
15_T19_A1.1040.55
281_D305_R1.1010.55
317_E347_D1.0800.54
329_Y332_G1.0800.54
240_Y263_Y1.0770.53
19_A23_D1.0680.53
60_N128_E1.0620.52
110_L114_D1.0610.52
84_D162_V1.0490.51
52_N76_I1.0380.51
55_T65_A1.0370.51
166_F175_I1.0340.50
44_G47_K1.0330.50
175_I207_I1.0150.49
86_N99_Y1.0090.49
42_T46_S1.0080.48
387_S390_V1.0060.48
126_T129_E1.0010.48
208_T249_A0.9990.48
141_R371_T0.9930.47
156_G177_N0.9880.47
224_D334_G0.9860.47
305_R334_G0.9840.47
386_L391_G0.9820.47
303_R332_G0.9820.47
209_I253_A0.9740.46
270_S289_L0.9730.46
163_G206_N0.9720.46
295_K317_E0.9710.46
309_V333_I0.9670.46
386_L389_S0.9650.46
112_A118_W0.9650.46
179_E416_A0.9650.46
44_G172_N0.9650.46
152_I166_F0.9630.45
47_K304_V0.9600.45
118_W126_T0.9580.45
112_A119_G0.9510.45
93_P132_A0.9490.44
304_V368_D0.9470.44
256_I414_A0.9470.44
363_G368_D0.9460.44
87_V330_A0.9440.44
341_A344_N0.9350.43
117_T121_K0.9350.43
218_D259_S0.9330.43
131_R134_S0.9320.43
180_F220_C0.9320.43
375_G378_I0.9310.43
161_V171_D0.9300.43
185_D204_Y0.9280.43
310_H313_N0.9230.43
180_F206_N0.9190.42
183_A206_N0.9150.42
92_K96_L0.9030.41
140_A143_M0.9020.41
221_T315_Y0.9000.41
208_T250_S0.8970.41
60_N364_T0.8940.41
160_K179_E0.8880.40
59_R83_I0.8850.40
213_T254_N0.8830.40
32_N37_A0.8820.40
23_D26_H0.8800.40
46_S76_I0.8770.39
356_T360_F0.8740.39
390_V393_T0.8720.39
223_N416_A0.8700.39
304_V334_G0.8650.39
18_G21_A0.8640.39
75_K150_T0.8570.38
137_N140_A0.8550.38
113_Y118_W0.8540.38
135_Q204_Y0.8520.38
43_T174_I0.8480.38
370_G374_N0.8460.37
337_S406_V0.8380.37
77_I143_M0.8370.37
379_N385_G0.8350.37
246_Q305_R0.8340.37
281_D334_G0.8330.37
138_Q204_Y0.8310.36
316_Y344_N0.8290.36
268_K305_R0.8280.36
95_G136_K0.8270.36
182_D223_N0.8270.36
156_G163_G0.8270.36
43_T46_S0.8260.36
253_A258_M0.8250.36
315_Y345_V0.8250.36
315_Y411_I0.8250.36
41_G214_H0.8250.36
343_N403_S0.8220.36
215_I256_I0.8200.36
91_L98_D0.8170.36
386_L392_W0.8160.35
276_D307_G0.8140.35
266_H296_N0.8090.35
173_V256_I0.8060.35
380_A383_A0.8050.35
267_D299_Q0.8050.35
60_N73_T0.7970.34
67_G71_N0.7960.34
152_I412_N0.7940.34
348_V416_A0.7930.34
141_R152_I0.7920.34
370_G375_G0.7920.34
315_Y407_K0.7910.34
175_I251_N0.7900.34
151_T254_N0.7880.34
26_H152_I0.7870.34
110_L117_T0.7870.34
155_S178_I0.7840.33
173_V213_T0.7830.33
291_H312_Y0.7830.33
401_D418_K0.7800.33
331_W371_T0.7710.33
71_N344_N0.7710.33
37_A214_H0.7700.33
304_V315_Y0.7700.33
224_D268_K0.7670.32
169_K211_G0.7660.32
167_Q208_T0.7660.32
159_A221_T0.7640.32
149_N347_D0.7630.32
173_V182_D0.7620.32
217_I413_Q0.7600.32
362_G366_L0.7590.32
57_S61_Q0.7570.32
404_A408_S0.7560.32
128_E134_S0.7560.32
396_L400_I0.7560.32
303_R309_V0.7510.31
291_H401_D0.7490.31
106_L110_L0.7490.31
100_K109_Y0.7480.31
304_V341_A0.7410.31
223_N264_H0.7400.31
414_A418_K0.7390.31
34_G45_G0.7350.30
379_N383_A0.7340.30
156_G161_V0.7340.30
262_Y296_N0.7330.30
113_Y125_G0.7330.30
347_D403_S0.7320.30
189_Q220_C0.7320.30
369_S390_V0.7280.30
54_Y73_T0.7250.30
100_K178_I0.7240.30
16_P316_Y0.7240.30
110_L119_G0.7240.30
109_Y222_F0.7230.30
301_A319_S0.7220.30
26_H29_L0.7180.29
148_A152_I0.7170.29
106_L114_D0.7170.29
210_N250_S0.7160.29
356_T364_T0.7150.29
110_L118_W0.7150.29
213_T256_I0.7140.29
79_I83_I0.7100.29
406_V413_Q0.7100.29
25_G78_Y0.7100.29
136_K139_K0.7080.29
60_N268_K0.7050.29
48_A175_I0.7050.29
26_H153_V0.7020.29
263_Y352_S0.7010.29
345_V379_N0.7000.28
82_T160_K0.7000.28
151_T224_D0.6990.28
170_S356_T0.6980.28
182_D224_D0.6970.28
125_G210_N0.6960.28
183_A272_F0.6950.28
48_A75_K0.6950.28
374_N378_I0.6940.28
364_T371_T0.6940.28
59_R62_L0.6880.28
174_I238_R0.6860.28
116_S119_G0.6850.28
42_T45_G0.6840.28
147_P151_T0.6830.27
99_Y125_G0.6830.27
17_A21_A0.6820.27
91_L95_G0.6820.27
374_N379_N0.6820.27
67_G145_D0.6810.27
174_I340_Y0.6790.27
60_N64_S0.6780.27
97_N105_D0.6740.27
191_D332_G0.6720.27
283_G337_S0.6710.27
387_S392_W0.6690.27
319_S349_P0.6690.27
88_D139_K0.6670.27
216_W257_T0.6640.26
119_G123_P0.6640.26
30_G176_R0.6630.26
203_Q299_Q0.6620.26
351_L407_K0.6610.26
351_L354_A0.6580.26
108_K112_A0.6550.26
281_D304_V0.6520.26
249_A287_I0.6510.26
61_Q277_S0.6490.26
189_Q338_K0.6480.26
163_G275_S0.6460.25
154_G344_N0.6450.25
121_K256_I0.6430.25
378_I381_S0.6430.25
124_S189_Q0.6430.25
112_A117_T0.6420.25
67_G73_T0.6420.25
296_N318_G0.6420.25
271_I305_R0.6420.25
58_N76_I0.6420.25
390_V395_S0.6390.25
330_A349_P0.6390.25
99_Y110_L0.6390.25
362_G402_A0.6390.25
391_G418_K0.6360.25
41_G48_A0.6340.25
211_G280_S0.6340.25
250_S274_S0.6330.25
160_K223_N0.6320.25
20_N23_D0.6310.25
33_D312_Y0.6300.25
92_K135_Q0.6240.24
412_N418_K0.6240.24
116_S126_T0.6230.24
166_F217_I0.6230.24
253_A316_Y0.6230.24
139_K238_R0.6190.24
267_D305_R0.6180.24
139_K203_Q0.6170.24
276_D296_N0.6170.24
172_N218_D0.6150.24
94_L98_D0.6150.24
133_R256_I0.6130.24
77_I345_V0.6120.24
366_L371_T0.6120.24
34_G44_G0.6100.24
313_N342_Q0.6090.24
86_N182_D0.6060.23
258_M289_L0.6050.23
82_T342_Q0.6050.23
399_S405_N0.6050.23
375_G379_N0.6040.23
359_V380_A0.6040.23
149_N173_V0.6020.23
391_G394_P0.6020.23
160_K178_I0.6010.23
36_G169_K0.6010.23
185_D267_D0.6000.23
58_N351_L0.5990.23
54_Y64_S0.5980.23
156_G417_G0.5980.23
146_I289_L0.5960.23
119_G125_G0.5960.23
140_A183_A0.5960.23
283_G306_F0.5950.23
279_T343_N0.5940.23
113_Y117_T0.5940.23
262_Y293_R0.5900.23
320_T375_G0.5900.23
349_P375_G0.5890.23
15_T20_N0.5890.23
75_K344_N0.5880.23
114_D151_T0.5880.23
75_K85_M0.5870.22
167_Q210_N0.5870.22
399_S408_S0.5860.22
138_Q337_S0.5850.22
36_G42_T0.5850.22
209_I339_I0.5840.22
268_K307_G0.5840.22
161_V164_G0.5840.22
269_S334_G0.5820.22
151_T171_D0.5820.22
23_D29_L0.5820.22
98_D112_A0.5820.22
182_D330_A0.5810.22
179_E261_N0.5810.22
104_Y110_L0.5810.22
213_T405_N0.5790.22
106_L158_N0.5780.22
368_D381_S0.5770.22
310_H315_Y0.5770.22
299_Q328_S0.5760.22
85_M163_G0.5760.22
150_T184_Y0.5740.22
25_G63_V0.5740.22
116_S121_K0.5740.22
175_I209_I0.5730.22
125_G128_E0.5730.22
59_R394_P0.5720.22
215_I405_N0.5720.22
281_D286_K0.5710.22
78_Y153_V0.5700.22
127_Q131_R0.5680.22
379_N382_A0.5680.22
342_Q370_G0.5680.22
136_K296_N0.5670.22
78_Y81_G0.5670.22
163_G184_Y0.5650.21
152_I402_A0.5640.21
341_A371_T0.5640.21
59_R150_T0.5610.21
31_S296_N0.5610.21
62_L172_N0.5600.21
84_D161_V0.5600.21
139_K274_S0.5600.21
139_K207_I0.5590.21
400_I403_S0.5590.21
77_I330_A0.5590.21
289_L294_Y0.5580.21
100_K105_D0.5580.21
118_W124_S0.5580.21
333_I363_G0.5560.21
135_Q165_N0.5560.21
46_S75_K0.5550.21
29_L153_V0.5540.21
413_Q417_G0.5520.21
254_N283_G0.5510.21
250_S281_D0.5510.21
317_E399_S0.5500.21
99_Y112_A0.5490.21
137_N182_D0.5480.21
18_G22_A0.5470.21
56_V273_G0.5440.20
224_D332_G0.5440.20
393_T397_H0.5430.20
380_A385_G0.5430.20
110_L125_G0.5430.20
248_D281_D0.5410.20
32_N141_R0.5410.20
129_E132_A0.5370.20
66_L79_I0.5360.20
279_T310_H0.5350.20
340_Y376_T0.5330.20
61_Q373_L0.5310.20
32_N36_G0.5300.20
116_S120_K0.5300.20
190_W248_D0.5300.20
71_N314_N0.5300.20
212_G254_N0.5290.20
153_V218_D0.5290.20
174_I218_D0.5290.20
180_F393_T0.5280.20
20_N52_N0.5270.20
77_I256_I0.5250.20
254_N287_I0.5250.20
79_I161_V0.5240.20
28_T153_V0.5240.20
32_N219_H0.5240.20
154_G205_D0.5230.20
82_T162_V0.5220.19
217_I406_V0.5190.19
275_S306_F0.5190.19
312_Y400_I0.5170.19
114_D126_T0.5150.19
249_A253_A0.5130.19
134_S138_Q0.5120.19
276_D320_T0.5110.19
248_D290_H0.5100.19
222_F316_Y0.5100.19
182_D334_G0.5090.19
263_Y295_K0.5090.19
114_D117_T0.5080.19
52_N72_T0.5060.19
287_I399_S0.5060.19
133_R137_N0.5050.19
47_K72_T0.5050.19
262_Y341_A0.5040.19
335_K412_N0.5040.19
94_L129_E0.5020.19
37_A52_N0.5020.19
139_K150_T0.5010.19
85_M184_Y0.5010.19
248_D273_G0.5000.19
72_T283_G0.5000.19
222_F253_A0.5000.19
208_T225_G0.5000.19
67_G168_I0.5000.19
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1vblA 1 0.9476 100 0.289 Contact Map
2o04A 1 0.9476 100 0.29 Contact Map
4hwvA 2 0.9714 100 0.317 Contact Map
1pe9A 1 0.8381 100 0.379 Contact Map
1pclA 1 0.8214 100 0.396 Contact Map
1idkA 1 0.8119 100 0.466 Contact Map
1qcxA 1 0.8119 100 0.473 Contact Map
3vmvA 1 0.7286 100 0.499 Contact Map
2qy1A 1 0.7381 100 0.505 Contact Map
1airA 1 0.7571 100 0.505 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.011 seconds.