RS1H - BACSU - GREMLIN Contacts

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RS1H - 30S ribosomal protein S1 homolog

UniProt:	P38494	Sequences: MTEEMNQIDVQVPEVGDVVKGIVTKVEDKHVDVEIINVKQSGIIPISELSSLHVEKASDVVKVDDELDLKVTKVEDDALILSKRAVDADRAWEDLEKKFETKEVFEAEVKDVVKGGLVVDIGVRGFIPASLVEAHFVEDFTDYKGKTLSLLVVELDRDKNRVILSHRAVVESEQANKKQELLQSLEVGSVLDGKVQRLTDFGAFVDIGGIDGLVHISQLSHSHVEKPSDVVEEGQEVKVKVLSVDRDNERISLSIKDTLPGPWNQIGEKVKPGDVLEGTVQRLVSFGAFVEILPGVEGLVHISQISNKHIGTPHEVLEEGQTVKVKVLDVNENEERISLSMRELEETPKADQEDYRQYQAKEETSTGFQLGDLIGDKLNKLK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	382 (332)
Sequences:	910 (501)
Seq/√Len:	27.5

ECOLI - RS1
BACSU - RS1H

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
192_D	236_E	7.259	1.00
192_D	238_K	5.351	1.00
219_L	231_V	5.119	1.00
232_E	235_Q	4.786	1.00
270_V	293_L	4.683	1.00
106_E	147_T	4.547	1.00
277_E	322_T	4.478	1.00
20_K	66_E	4.423	1.00
186_E	189_S	4.292	1.00
83_K	87_D	4.233	1.00
254_S	257_D	4.230	1.00
271_K	274_D	4.219	1.00
102_K	151_L	4.027	1.00
214_V	219_L	3.561	1.00
277_E	324_K	3.456	1.00
278_G	290_V	3.274	1.00
318_E	321_Q	3.184	1.00
331_N	334_E	3.088	1.00
269_K	293_L	3.027	1.00
214_V	255_I	2.922	1.00
280_V	290_V	2.910	1.00
237_V	255_I	2.909	1.00
62_K	65_D	2.852	1.00
107_A	150_L	2.641	0.99
203_A	231_V	2.569	0.99
261_G	335_E	2.377	0.99
22_I	66_E	2.297	0.98
51_S	58_S	2.245	0.98
187_V	243_S	2.201	0.98
198_L	203_A	2.189	0.98
245_D	248_N	2.106	0.97
305_I	317_L	2.097	0.97
23_V	61_V	2.082	0.97
96_E	100_E	2.029	0.96
226_K	229_D	1.992	0.96
20_K	68_D	1.941	0.95
340_S	343_E	1.932	0.95
180_E	183_Q	1.924	0.95
117_L	140_F	1.869	0.94
178_K	249_E	1.855	0.94
26_V	59_D	1.853	0.94
279_T	322_T	1.843	0.94
106_E	149_S	1.833	0.94
125_G	164_L	1.811	0.93
97_K	101_T	1.785	0.93
109_V	117_L	1.769	0.93
206_D	209_G	1.760	0.92
133_E	143_Y	1.747	0.92
218_Q	254_S	1.736	0.92
33_V	44_I	1.720	0.91
105_F	150_L	1.692	0.91
98_K	103_E	1.688	0.91
280_V	300_V	1.683	0.90
90_R	93_E	1.681	0.90
196_Q	211_D	1.671	0.90
194_K	236_E	1.671	0.90
291_E	294_P	1.643	0.89
328_L	340_S	1.579	0.87
176_N	180_E	1.525	0.85
108_E	147_T	1.473	0.83
149_S	166_H	1.471	0.83
134_A	174_Q	1.464	0.83
231_V	255_I	1.462	0.83
190_V	238_K	1.440	0.82
180_E	184_S	1.435	0.82
300_V	341_M	1.419	0.81
307_N	342_R	1.414	0.81
219_L	230_V	1.413	0.81
137_V	300_V	1.403	0.80
34_E	41_S	1.396	0.80
131_L	144_K	1.394	0.80
167_R	174_Q	1.392	0.80
310_I	315_E	1.383	0.79
166_H	170_V	1.383	0.79
327_V	330_V	1.310	0.75
117_L	143_Y	1.307	0.75
31_V	44_I	1.291	0.74
44_I	69_L	1.283	0.74
241_V	244_V	1.281	0.74
21_G	33_V	1.279	0.74
205_V	231_V	1.279	0.74
175_A	179_Q	1.278	0.73
344_L	348_P	1.263	0.73
271_K	327_V	1.253	0.72
109_V	143_Y	1.250	0.72
224_V	244_V	1.244	0.71
270_V	292_I	1.240	0.71
153_V	198_L	1.239	0.71
266_I	330_V	1.215	0.70
307_N	348_P	1.215	0.70
124_R	153_V	1.201	0.69
34_E	77_D	1.199	0.69
342_R	348_P	1.188	0.68
151_L	172_S	1.173	0.67
107_A	127_I	1.172	0.67
181_L	210_I	1.172	0.67
154_E	201_F	1.170	0.67
23_V	26_V	1.163	0.66
97_K	100_E	1.156	0.66
199_T	211_D	1.155	0.66
119_V	150_L	1.152	0.65
94_D	103_E	1.143	0.65
177_K	180_E	1.140	0.65
185_L	189_S	1.137	0.64
305_I	340_S	1.134	0.64
95_L	181_L	1.120	0.63
205_V	214_V	1.109	0.62
193_G	205_V	1.101	0.62
127_I	150_L	1.101	0.62
198_L	235_Q	1.097	0.62
51_S	57_A	1.088	0.61
68_D	90_R	1.084	0.61
186_E	246_R	1.080	0.60
173_E	176_N	1.079	0.60
107_A	119_V	1.078	0.60
203_A	230_V	1.076	0.60
172_S	176_N	1.072	0.60
196_Q	199_T	1.068	0.59
224_V	230_V	1.058	0.59
50_S	59_D	1.054	0.58
49_L	67_L	1.042	0.57
213_L	289_F	1.038	0.57
265_Q	300_V	1.030	0.57
310_I	313_P	1.030	0.57
146_K	152_V	1.028	0.56
117_L	142_D	1.028	0.56
131_L	153_V	1.022	0.56
242_L	257_D	1.017	0.56
131_L	174_Q	1.011	0.55
162_V	231_V	1.005	0.55
179_Q	183_Q	0.996	0.54
46_I	50_S	0.995	0.54
44_I	296_V	0.990	0.54
113_V	250_R	0.984	0.53
217_S	286_F	0.983	0.53
212_G	253_L	0.979	0.53
112_V	286_F	0.975	0.52
148_L	296_V	0.974	0.52
104_V	149_S	0.969	0.52
312_T	315_E	0.967	0.52
326_K	340_S	0.963	0.52
221_H	261_G	0.960	0.51
254_S	282_R	0.960	0.51
197_R	207_I	0.956	0.51
98_K	152_V	0.952	0.51
127_I	164_L	0.948	0.50
230_V	255_I	0.948	0.50
88_A	201_F	0.947	0.50
266_I	282_R	0.940	0.50
274_D	330_V	0.938	0.50
104_V	169_V	0.935	0.49
342_R	347_T	0.933	0.49
298_G	330_V	0.932	0.49
161_R	166_H	0.932	0.49
225_E	229_D	0.932	0.49
214_V	231_V	0.929	0.49
218_Q	221_H	0.928	0.49
148_L	283_L	0.925	0.49
100_E	195_V	0.917	0.48
159_K	167_R	0.909	0.47
260_P	265_Q	0.909	0.47
99_F	152_V	0.906	0.47
283_L	313_P	0.905	0.47
290_V	300_V	0.903	0.47
198_L	227_P	0.899	0.47
137_V	280_V	0.893	0.46
194_K	268_E	0.886	0.46
218_Q	257_D	0.885	0.46
35_I	48_E	0.883	0.45
82_S	86_V	0.882	0.45
275_V	326_K	0.880	0.45
270_V	327_V	0.880	0.45
142_D	146_K	0.877	0.45
77_D	80_I	0.877	0.45
136_F	197_R	0.877	0.45
275_V	324_K	0.876	0.45
127_I	161_R	0.875	0.45
22_I	34_E	0.875	0.45
117_L	132_V	0.875	0.45
112_V	129_A	0.873	0.45
75_E	92_W	0.873	0.45
27_E	32_D	0.867	0.44
84_R	88_A	0.865	0.44
138_E	205_V	0.860	0.44
302_I	305_I	0.857	0.44
232_E	292_I	0.851	0.43
25_K	185_L	0.851	0.43
154_E	161_R	0.849	0.43
283_L	312_T	0.845	0.43
300_V	325_V	0.844	0.43
219_L	274_D	0.841	0.42
134_A	170_V	0.838	0.42
204_F	213_L	0.834	0.42
203_A	214_V	0.833	0.42
69_L	74_V	0.832	0.42
285_S	327_V	0.830	0.42
148_L	166_H	0.830	0.42
214_V	237_V	0.829	0.41
196_Q	214_V	0.823	0.41
214_V	274_D	0.823	0.41
107_A	136_F	0.823	0.41
168_A	171_E	0.820	0.41
217_S	254_S	0.819	0.41
299_L	303_S	0.818	0.41
135_H	161_R	0.816	0.40
105_F	327_V	0.815	0.40
123_V	245_D	0.812	0.40
279_T	291_E	0.811	0.40
306_S	342_R	0.810	0.40
113_V	134_A	0.810	0.40
291_E	328_L	0.809	0.40
239_V	250_R	0.807	0.40
272_P	329_D	0.803	0.40
212_G	239_V	0.802	0.39
260_P	264_N	0.800	0.39
30_H	43_I	0.798	0.39
93_E	96_E	0.796	0.39
26_V	63_V	0.794	0.39
183_Q	296_V	0.791	0.39
181_L	206_D	0.785	0.38
200_D	228_S	0.784	0.38
28_D	241_V	0.781	0.38
167_R	248_N	0.780	0.38
288_A	317_L	0.779	0.38
283_L	310_I	0.778	0.38
221_H	257_D	0.777	0.38
76_D	243_S	0.777	0.38
218_Q	328_L	0.773	0.37
167_R	294_P	0.771	0.37
74_V	79_L	0.770	0.37
185_L	336_R	0.767	0.37
78_A	167_R	0.765	0.37
163_I	218_Q	0.761	0.37
281_Q	297_E	0.760	0.36
143_Y	149_S	0.759	0.36
127_I	166_H	0.759	0.36
46_I	59_D	0.759	0.36
181_L	184_S	0.759	0.36
283_L	317_L	0.753	0.36
96_E	157_R	0.751	0.36
156_D	159_K	0.749	0.36
274_D	327_V	0.742	0.35
43_I	47_S	0.742	0.35
23_V	31_V	0.741	0.35
252_S	336_R	0.740	0.35
130_S	339_L	0.738	0.35
329_D	338_S	0.734	0.35
208_G	211_D	0.732	0.34
195_V	219_L	0.727	0.34
283_L	286_F	0.727	0.34
115_G	289_F	0.726	0.34
84_R	241_V	0.723	0.34
204_F	211_D	0.723	0.34
121_I	179_Q	0.722	0.34
82_S	85_A	0.720	0.34
191_L	210_I	0.717	0.33
278_G	338_S	0.716	0.33
184_S	191_L	0.716	0.33
44_I	67_L	0.715	0.33
298_G	301_H	0.714	0.33
213_L	252_S	0.713	0.33
301_H	313_P	0.712	0.33
47_S	164_L	0.711	0.33
102_K	106_E	0.709	0.33
181_L	185_L	0.708	0.33
89_D	93_E	0.707	0.33
185_L	205_V	0.704	0.33
43_I	160_N	0.701	0.32
34_E	122_G	0.700	0.32
92_W	155_L	0.699	0.32
234_G	241_V	0.699	0.32
120_D	335_E	0.697	0.32
222_S	282_R	0.696	0.32
275_V	344_L	0.696	0.32
243_S	277_E	0.695	0.32
199_T	282_R	0.693	0.32
27_E	30_H	0.692	0.32
139_D	142_D	0.689	0.32
242_L	254_S	0.689	0.32
176_N	179_Q	0.688	0.32
13_P	38_V	0.686	0.31
113_V	166_H	0.685	0.31
124_R	160_N	0.685	0.31
125_G	150_L	0.685	0.31
154_E	286_F	0.684	0.31
219_L	227_P	0.682	0.31
276_L	325_V	0.681	0.31
27_E	67_L	0.678	0.31
150_L	164_L	0.678	0.31
158_D	285_S	0.677	0.31
13_P	41_S	0.677	0.31
142_D	205_V	0.676	0.31
118_V	187_V	0.676	0.31
14_E	17_D	0.676	0.31
155_L	162_V	0.675	0.31
215_H	227_P	0.674	0.31
90_R	156_D	0.674	0.31
94_D	97_K	0.674	0.31
236_E	251_I	0.673	0.31
261_G	264_N	0.672	0.31
292_I	337_I	0.671	0.30
30_H	46_I	0.671	0.30
97_K	180_E	0.670	0.30
71_V	205_V	0.669	0.30
84_R	247_D	0.666	0.30
282_R	330_V	0.664	0.30
249_E	292_I	0.662	0.30
250_R	270_V	0.661	0.30
205_V	290_V	0.659	0.30
175_A	296_V	0.658	0.30
305_I	313_P	0.657	0.30
77_D	183_Q	0.656	0.29
98_K	105_F	0.656	0.29
80_I	236_E	0.655	0.29
23_V	46_I	0.655	0.29
250_R	258_T	0.655	0.29
206_D	211_D	0.651	0.29
144_K	163_I	0.650	0.29
308_K	342_R	0.650	0.29
304_Q	340_S	0.649	0.29
197_R	328_L	0.647	0.29
224_V	278_G	0.644	0.29
133_E	166_H	0.642	0.29
131_L	160_N	0.642	0.29
104_V	151_L	0.640	0.28
70_K	75_E	0.640	0.28
266_I	270_V	0.640	0.28
118_V	254_S	0.639	0.28
291_E	295_G	0.635	0.28
76_D	231_V	0.634	0.28
101_T	205_V	0.634	0.28
191_L	239_V	0.633	0.28
127_I	286_F	0.633	0.28
124_R	233_E	0.631	0.28
105_F	163_I	0.630	0.28
207_I	225_E	0.630	0.28
139_D	279_T	0.629	0.28
131_L	168_A	0.629	0.28
286_F	301_H	0.629	0.28
137_V	291_E	0.628	0.28
51_S	60_V	0.628	0.28
197_R	282_R	0.625	0.28
150_L	166_H	0.623	0.27
221_H	247_D	0.622	0.27
319_E	327_V	0.621	0.27
155_L	292_I	0.621	0.27
284_V	289_F	0.620	0.27
278_G	328_L	0.620	0.27
125_G	160_N	0.619	0.27
230_V	274_D	0.619	0.27
162_V	255_I	0.618	0.27
102_K	121_I	0.617	0.27
308_K	315_E	0.616	0.27
308_K	340_S	0.616	0.27
156_D	325_V	0.616	0.27
211_D	227_P	0.616	0.27
131_L	299_L	0.616	0.27
164_L	283_L	0.615	0.27
240_K	257_D	0.614	0.27
304_Q	328_L	0.614	0.27
25_K	266_I	0.613	0.27
107_A	161_R	0.612	0.27
283_L	319_E	0.611	0.27
153_V	270_V	0.609	0.27
82_S	92_W	0.608	0.27
18_V	34_E	0.604	0.26
104_V	184_S	0.604	0.26
243_S	256_K	0.602	0.26
140_F	153_V	0.599	0.26
153_V	293_L	0.598	0.26
309_H	315_E	0.597	0.26
278_G	288_A	0.597	0.26
216_I	299_L	0.597	0.26
223_H	309_H	0.597	0.26
14_E	25_K	0.596	0.26
31_V	317_L	0.596	0.26
289_F	299_L	0.595	0.26
320_G	334_E	0.595	0.26
121_I	317_L	0.594	0.26
210_I	247_D	0.594	0.26
129_A	197_R	0.594	0.26
84_R	201_F	0.592	0.26
19_V	210_I	0.592	0.26
277_E	280_V	0.591	0.26
197_R	254_S	0.590	0.25
29_K	41_S	0.590	0.25
56_K	59_D	0.588	0.25
95_L	156_D	0.584	0.25
300_V	323_V	0.584	0.25
223_H	227_P	0.583	0.25
96_E	155_L	0.580	0.25
218_Q	258_T	0.580	0.25
132_V	182_L	0.579	0.25
201_F	302_I	0.577	0.25
26_V	46_I	0.577	0.25
226_K	331_N	0.576	0.25
130_S	299_L	0.574	0.25
117_L	150_L	0.570	0.24
125_G	162_V	0.570	0.24
307_N	347_T	0.570	0.24
20_K	62_K	0.569	0.24
242_L	297_E	0.567	0.24
237_V	258_T	0.567	0.24
201_F	208_G	0.562	0.24
25_K	32_D	0.560	0.24
151_L	168_A	0.560	0.24
308_K	347_T	0.560	0.24
178_K	241_V	0.559	0.24
129_A	207_I	0.557	0.24
95_L	144_K	0.556	0.24
201_F	304_Q	0.555	0.24
154_E	284_V	0.553	0.23
91_A	210_I	0.553	0.23
259_L	279_T	0.552	0.23
214_V	258_T	0.551	0.23
21_G	198_L	0.550	0.23
162_V	321_Q	0.549	0.23
216_I	223_H	0.548	0.23
145_G	276_L	0.548	0.23
156_D	282_R	0.547	0.23
34_E	68_D	0.547	0.23
311_G	341_M	0.546	0.23
43_I	327_V	0.546	0.23
199_T	227_P	0.546	0.23
261_G	330_V	0.544	0.23
102_K	183_Q	0.543	0.23
312_T	335_E	0.542	0.23
172_S	235_Q	0.542	0.23
191_L	255_I	0.540	0.23
48_E	198_L	0.540	0.23
90_R	222_S	0.540	0.23
91_A	184_S	0.539	0.23
183_Q	186_E	0.539	0.23
133_E	178_K	0.538	0.23
85_A	311_G	0.538	0.23
183_Q	277_E	0.536	0.22
168_A	336_R	0.536	0.22
177_K	220_S	0.536	0.22
271_K	330_V	0.535	0.22
96_E	132_V	0.535	0.22
137_V	142_D	0.534	0.22
109_V	132_V	0.532	0.22
22_I	139_D	0.532	0.22
184_S	241_V	0.531	0.22
113_V	169_V	0.529	0.22
310_I	345_E	0.529	0.22
282_R	286_F	0.529	0.22
84_R	167_R	0.527	0.22
268_E	274_D	0.527	0.22
311_G	343_E	0.526	0.22
14_E	327_V	0.526	0.22
343_E	347_T	0.526	0.22
75_E	182_L	0.526	0.22
133_E	137_V	0.525	0.22
201_F	213_L	0.524	0.22
207_I	251_I	0.524	0.22
265_Q	269_K	0.524	0.22
190_V	240_K	0.523	0.22
143_Y	152_V	0.521	0.22
124_R	203_A	0.520	0.22
203_A	227_P	0.520	0.22
139_D	164_L	0.518	0.22
129_A	217_S	0.518	0.22
132_V	139_D	0.518	0.22
153_V	179_Q	0.518	0.22
278_G	339_L	0.518	0.22
99_F	189_S	0.518	0.22
127_I	307_N	0.517	0.22
136_F	159_K	0.515	0.21
25_K	47_S	0.515	0.21
325_V	328_L	0.515	0.21
237_V	246_R	0.514	0.21
281_Q	335_E	0.514	0.21
70_K	174_Q	0.514	0.21
90_R	249_E	0.514	0.21
219_L	247_D	0.513	0.21
298_G	331_N	0.512	0.21
316_V	337_I	0.510	0.21
133_E	170_V	0.510	0.21
131_L	309_H	0.509	0.21
296_V	332_E	0.508	0.21
299_L	339_L	0.507	0.21
253_L	289_F	0.507	0.21
46_I	56_K	0.506	0.21
187_V	260_P	0.506	0.21
141_T	195_V	0.506	0.21
159_K	270_V	0.505	0.21
79_L	139_D	0.505	0.21
195_V	267_G	0.504	0.21
182_L	241_V	0.504	0.21
19_V	28_D	0.504	0.21
189_S	246_R	0.503	0.21
23_V	60_V	0.503	0.21
105_F	166_H	0.502	0.21
31_V	46_I	0.502	0.21
35_I	42_G	0.502	0.21
99_F	155_L	0.501	0.21
224_V	280_V	0.500	0.21

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4q7jD	1	0.445	100	0.55	Contact Map
3go5A	1	0.7173	100	0.64	Contact Map
1q8kA	1	0.5131	100	0.739	Contact Map
1hh2P	2	0.7775	100	0.755	Contact Map
2k4kA	1	0.3325	100	0.758	Contact Map
4aidA	3	0.3089	100	0.763	Contact Map
3cdiA	3	0.233	100	0.765	Contact Map
4nngA	1	0.4058	100	0.769	Contact Map
3aevA	1	0.3141	100	0.77	Contact Map
4nbqA	3	0.5916	100	0.77	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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