GREMLIN Database
ISCS - Putative cysteine desulfurase NifS
UniProt: P38033 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 395 (335)
Sequences: 27180 (18606)
Seq/√Len: 1016.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
268_Y364_E3.4031.00
90_H116_T3.2611.00
146_H178_T3.2221.00
268_Y360_A3.2181.00
272_Q367_L3.2071.00
161_S171_L3.1311.00
51_K249_E2.9411.00
156_P159_H2.8161.00
175_A194_L2.7721.00
132_L163_Y2.7591.00
95_A119_E2.6231.00
246_A249_E2.4361.00
196_M209_A2.4131.00
185_N188_N2.3971.00
237_N240_G2.3651.00
90_H136_R2.3121.00
315_S372_V2.2691.00
90_H114_D2.2391.00
132_L166_N2.2141.00
164_L169_I2.2031.00
21_Q25_M2.1761.00
142_V164_L2.1241.00
268_Y272_Q2.0991.00
14_E18_T2.0691.00
57_G185_N2.0691.00
272_Q364_E2.0581.00
352_I366_L2.0281.00
130_I133_T1.9861.00
147_A292_L1.9821.00
264_A360_A1.9621.00
272_Q276_R1.9381.00
281_T299_F1.9151.00
158_Q188_N1.9121.00
315_S369_T1.9001.00
90_H139_T1.8871.00
116_T134_H1.8761.00
17_L21_Q1.8661.00
281_T301_H1.8641.00
119_E130_I1.8551.00
22_K26_D1.8381.00
67_S71_E1.8321.00
283_A299_F1.8221.00
130_I134_H1.8121.00
51_K245_T1.8111.00
144_I160_L1.7991.00
121_N125_L1.7781.00
54_N252_V1.7631.00
365_Q369_T1.7581.00
14_E25_M1.7531.00
89_R138_E1.7351.00
173_C191_V1.7231.00
162_S166_N1.7081.00
73_N100_S1.7071.00
158_Q162_S1.7031.00
127_T130_I1.6571.00
261_R265_L1.6041.00
269_F354_F1.6041.00
90_H138_E1.5801.00
164_L171_L1.5711.00
198_S244_F1.5531.00
82_N111_Q1.5431.00
66_T72_S1.5211.00
128_E163_Y1.5071.00
62_G214_P1.5001.00
51_K252_V1.4891.00
277_S371_T1.4841.00
191_V194_L1.4811.00
165_H190_G1.4731.00
69_G176_V1.4611.00
19_V246_A1.4571.00
163_Y167_K1.4541.00
297_G349_F1.4531.00
135_I163_Y1.4511.00
20_Y243_A1.4481.00
259_I263_E1.4471.00
73_N172_H1.4391.00
18_T22_K1.4341.00
95_A117_V1.4291.00
162_S190_G1.4221.00
76_A193_A1.4181.00
47_Y245_T1.4131.00
107_F223_P1.4081.00
267_T271_D1.4071.00
159_H162_S1.3911.00
304_E307_Y1.3901.00
272_Q371_T1.3841.00
18_T21_Q1.3831.00
78_Q223_P1.3771.00
27_M40_K1.3711.00
204_P243_A1.3701.00
157_I173_C1.3681.00
165_H192_D1.3681.00
266_R270_L1.3681.00
89_R140_G1.3631.00
59_E187_K1.3601.00
161_S190_G1.3571.00
256_E260_S1.3561.00
93_T105_A1.3561.00
263_E267_T1.3521.00
19_V22_K1.3451.00
311_E373_L1.3361.00
128_E159_H1.3361.00
311_E314_R1.3361.00
141_L172_H1.3301.00
48_C245_T1.3251.00
163_Y166_N1.3251.00
193_A212_I1.3041.00
15_E250_L1.3031.00
17_L25_M1.3001.00
47_Y51_K1.2871.00
270_L296_I1.2811.00
269_F363_L1.2681.00
129_E133_T1.2681.00
276_R371_T1.2641.00
50_E54_N1.2621.00
129_E132_L1.2511.00
202_H355_G1.2511.00
118_I134_H1.2501.00
360_A364_E1.2461.00
201_I244_F1.2451.00
270_L274_R1.2411.00
61_S214_P1.2371.00
121_N127_T1.2361.00
80_L170_L1.2331.00
319_I350_I1.2281.00
55_I179_F1.2281.00
254_E257_K1.2241.00
260_S264_A1.2181.00
147_A178_T1.2141.00
144_I157_I1.2121.00
179_F196_M1.2091.00
93_T117_V1.2021.00
44_I242_G1.1941.00
131_L142_V1.1911.00
92_I134_H1.1851.00
123_Y285_D1.1801.00
88_K140_G1.1771.00
317_I365_Q1.1751.00
369_T372_V1.1711.00
132_L167_K1.1641.00
279_P301_H1.1561.00
142_V169_I1.1501.00
270_L291_C1.1461.00
365_Q368_H1.1431.00
144_I171_L1.1411.00
178_T194_L1.1291.00
59_E214_P1.1271.00
128_E132_L1.1231.00
72_S195_S1.1231.00
106_A109_E1.1211.00
245_T249_E1.1171.00
367_L371_T1.1121.00
45_L241_I1.1121.00
131_L160_L1.1111.00
265_L363_L1.1101.00
114_D136_R1.1091.00
52_I209_A1.1011.00
132_L136_R1.0991.00
43_H47_Y1.0971.00
285_D288_K1.0971.00
146_H194_L1.0921.00
186_T211_Y1.0911.00
359_T362_Q1.0901.00
57_G187_K1.0851.00
50_E53_A1.0841.00
277_S280_V1.0781.00
154_I182_I1.0751.00
96_M155_Q1.0681.00
213_R216_V1.0681.00
125_L290_E1.0621.00
183_P256_E1.0621.00
143_S172_H1.0611.00
50_E60_A1.0591.00
271_D274_R1.0591.00
63_I211_Y1.0581.00
172_H195_S1.0441.00
102_H117_V1.0431.00
51_K54_N1.0421.00
272_Q368_H1.0371.00
103_N106_A1.0301.00
91_F105_A1.0231.00
92_I139_T1.0181.00
161_S165_H1.0161.00
165_H171_L1.0091.00
311_E315_S1.0011.00
266_R296_I0.9981.00
59_E62_G0.9981.00
136_R139_T0.9971.00
246_A250_L0.9921.00
146_H189_L0.9911.00
81_L113_Y0.9881.00
55_I248_A0.9881.00
170_L193_A0.9851.00
193_A216_V0.9761.00
132_L159_H0.9631.00
101_I143_S0.9631.00
317_I366_L0.9631.00
136_R167_K0.9611.00
52_I248_A0.9591.00
78_Q107_F0.9541.00
254_E258_Q0.9531.00
368_H372_V0.9481.00
258_Q356_R0.9391.00
56_I196_M0.9371.00
270_L282_L0.9291.00
261_R264_A0.9281.00
17_L24_S0.9251.00
269_F366_L0.9241.00
135_I139_T0.9221.00
192_D213_R0.9221.00
36_D40_K0.9201.00
201_I248_A0.9181.00
309_M321_T0.9151.00
285_D290_E0.9131.00
257_K260_S0.9121.00
298_C352_I0.9071.00
207_V241_I0.9041.00
198_S207_V0.9001.00
56_I211_Y0.8981.00
257_K261_R0.8981.00
364_E368_H0.8971.00
265_L360_A0.8931.00
164_L167_K0.8931.00
249_E253_S0.8931.00
312_C319_I0.8921.00
269_F296_I0.8921.00
146_H184_I0.8871.00
310_L313_N0.8871.00
109_E117_V0.8861.00
354_F363_L0.8831.00
107_F110_Q0.8821.00
317_I369_T0.8801.00
361_E364_E0.8771.00
77_I105_A0.8741.00
258_Q262_N0.8721.00
258_Q261_R0.8701.00
78_Q82_N0.8651.00
358_T363_L0.8641.00
128_E131_L0.8581.00
361_E365_Q0.8541.00
283_A297_G0.8541.00
274_R285_D0.8541.00
16_A247_A0.8531.00
283_A349_F0.8531.00
53_A58_G0.8521.00
219_K223_P0.8491.00
198_S203_G0.8481.00
158_Q189_L0.8421.00
273_I298_C0.8401.00
172_H193_A0.8331.00
37_A318_C0.8331.00
58_G62_G0.8331.00
319_I352_I0.8301.00
307_Y311_E0.8291.00
142_V160_L0.8271.00
145_Q149_S0.8261.00
80_L193_A0.8261.00
47_Y249_E0.8171.00
40_K43_H0.8171.00
146_H157_I0.8151.00
48_C51_K0.8131.00
179_F248_A0.8081.00
170_L216_V0.8071.00
20_Y24_S0.8071.00
119_E134_H0.8041.00
309_M319_I0.8011.00
223_P226_T0.8011.00
306_Q310_L0.7991.00
95_A120_P0.7961.00
190_G213_R0.7911.00
221_V226_T0.7831.00
164_L168_G0.7831.00
178_T182_I0.7811.00
125_L155_Q0.7801.00
64_Y75_L0.7791.00
179_F201_I0.7781.00
185_N189_L0.7761.00
207_V240_G0.7661.00
116_T136_R0.7651.00
89_R114_D0.7621.00
269_F352_I0.7611.00
179_F184_I0.7561.00
269_F367_L0.7511.00
76_A210_V0.7461.00
73_N195_S0.7451.00
36_D313_N0.7451.00
106_A117_V0.7431.00
161_S189_L0.7431.00
131_L135_I0.7421.00
108_L111_Q0.7401.00
116_T139_T0.7391.00
366_L370_F0.7361.00
94_T144_I0.7351.00
358_T362_Q0.7331.00
15_E253_S0.7321.00
102_H106_A0.7321.00
202_H356_R0.7311.00
44_I47_Y0.7291.00
175_A179_F0.7281.00
51_K248_A0.7231.00
220_P223_P0.7221.00
368_H371_T0.7181.00
51_K55_I0.7171.00
220_P224_L0.7141.00
280_V300_F0.7141.00
92_I116_T0.7121.00
303_F308_V0.7111.00
22_K27_M0.7111.00
71_E222_Y0.7111.00
218_W222_Y0.7101.00
299_F348_Q0.7091.00
55_I252_V0.7091.00
56_I186_T0.7081.00
267_T288_K0.7081.00
45_L238_V0.7021.00
91_F141_L0.7021.00
48_C241_I0.7011.00
221_V224_L0.6991.00
93_T143_S0.6961.00
284_A291_C0.6951.00
15_E18_T0.6941.00
62_G187_K0.6941.00
15_E246_A0.6931.00
175_A178_T0.6931.00
135_I167_K0.6911.00
145_Q155_Q0.6901.00
23_L44_I0.6871.00
170_L192_D0.6871.00
24_S239_P0.6851.00
264_A268_Y0.6821.00
135_I169_I0.6811.00
222_Y226_T0.6801.00
183_P259_I0.6801.00
42_K46_E0.6781.00
266_R291_C0.6781.00
290_E293_P0.6721.00
58_G211_Y0.6721.00
291_C295_I0.6721.00
292_L295_I0.6691.00
52_I65_F0.6671.00
114_D138_E0.6621.00
78_Q221_V0.6611.00
310_L314_R0.6581.00
35_H38_G0.6581.00
161_S173_C0.6581.00
22_K25_M0.6571.00
308_V370_F0.6571.00
146_H154_I0.6551.00
75_L79_S0.6541.00
105_A143_S0.6491.00
29_G32_S0.6491.00
184_I188_N0.6481.00
47_Y50_E0.6461.00
93_T97_E0.6461.00
107_F111_Q0.6461.00
94_T97_E0.6451.00
109_E113_Y0.6451.00
121_N130_I0.6421.00
93_T115_V0.6391.00
308_V373_L0.6361.00
76_A172_H0.6361.00
15_E254_E0.6361.00
14_E21_Q0.6321.00
176_V197_S0.6311.00
157_I171_L0.6311.00
161_S191_V0.6311.00
128_E160_L0.6271.00
99_Q103_N0.6261.00
272_Q277_S0.6251.00
268_Y363_L0.6241.00
90_H137_P0.6241.00
222_Y225_T0.6231.00
268_Y271_D0.6231.00
366_L369_T0.6211.00
307_Y310_L0.6201.00
146_H173_C0.6201.00
209_A244_F0.6171.00
14_E17_L0.6091.00
137_P167_K0.6091.00
227_H230_G0.6081.00
183_P255_M0.6071.00
235_T238_V0.6061.00
355_G358_T0.6041.00
295_I353_S0.6031.00
19_V242_G0.6021.00
221_V225_T0.6011.00
105_A115_V0.5991.00
233_A236_V0.5991.00
305_G309_M0.5991.00
234_G239_P0.5981.00
300_F308_V0.5961.00
68_G208_G0.5941.00
364_E367_L0.5931.00
210_V213_R0.5931.00
296_I299_F0.5931.00
269_F273_I0.5901.00
255_M259_I0.5881.00
69_G195_S0.5871.00
147_A154_I0.5871.00
233_A238_V0.5851.00
49_R228_E0.5831.00
194_L211_Y0.5831.00
69_G73_N0.5831.00
131_L163_Y0.5831.00
96_M120_P0.5831.00
158_Q190_G0.5821.00
132_L135_I0.5811.00
56_I179_F0.5811.00
118_I130_I0.5801.00
16_A246_A0.5801.00
209_A212_I0.5791.00
285_D289_A0.5791.00
217_P220_P0.5791.00
109_E115_V0.5771.00
92_I118_I0.5771.00
23_L242_G0.5761.00
299_F345_E0.5731.00
46_E49_R0.5731.00
104_C223_P0.5731.00
228_E232_R0.5711.00
300_F374_W0.5701.00
234_G237_N0.5701.00
251_I258_Q0.5691.00
62_G213_R0.5681.00
162_S165_H0.5671.00
159_H163_Y0.5671.00
30_N33_S0.5661.00
161_S164_L0.5661.00
18_T25_M0.5661.00
184_I187_K0.5661.00
251_I255_M0.5651.00
198_S201_I0.5651.00
53_A60_A0.5641.00
19_V23_L0.5641.00
145_Q148_N0.5621.00
28_Y31_A0.5621.00
32_S236_V0.5591.00
170_L215_D0.5591.00
249_E252_V0.5581.00
295_I299_F0.5581.00
271_D276_R0.5581.00
200_K351_R0.5541.00
58_G194_L0.5531.00
273_I367_L0.5521.00
232_R236_V0.5511.00
74_F78_Q0.5501.00
125_L285_D0.5491.00
118_I131_L0.5491.00
78_Q81_L0.5491.00
184_I194_L0.5481.00
269_F272_Q0.5471.00
75_L221_V0.5461.00
369_T373_L0.5451.00
54_N57_G0.5451.00
201_I251_I0.5451.00
20_Y239_P0.5431.00
260_S263_E0.5411.00
160_L171_L0.5391.00
303_F307_Y0.5391.00
23_L27_M0.5391.00
272_Q363_L0.5371.00
45_L236_V0.5371.00
125_L153_I0.5301.00
300_F370_F0.5301.00
268_Y367_L0.5301.00
186_T189_L0.5281.00
157_I161_S0.5271.00
261_R360_A0.5271.00
219_K222_Y0.5271.00
23_L239_P0.5271.00
250_L356_R0.5241.00
186_T191_V0.5231.00
273_I280_V0.5221.00
265_L358_T0.5211.00
245_T248_A0.5211.00
186_T190_G0.5201.00
263_E266_R0.5191.00
305_G321_T0.5161.00
277_S367_L0.5131.00
193_A210_V0.5131.00
165_H169_I0.5131.00
218_W221_V0.5121.00
187_K190_G0.5111.00
22_K44_I0.5111.00
248_A252_V0.5101.00
265_L354_F0.5091.00
236_V239_P0.5091.00
101_I105_A0.5091.00
202_H251_I0.5081.00
58_G186_T0.5081.00
70_T74_F0.5071.00
259_I262_N0.5071.00
44_I245_T0.5061.00
232_R238_V0.5061.00
264_A267_T0.5041.00
121_N124_G0.5041.00
234_G238_V0.5031.00
171_L191_V0.5031.00
313_N318_C0.5031.00
220_P225_T0.5011.00
217_P221_V0.5011.00
109_E114_D0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4isyA 2 0.9544 100 0.39 Contact Map
1eg5A 2 0.9139 100 0.393 Contact Map
4hvkA 2 0.9342 100 0.397 Contact Map
3e9kA 2 0.8785 100 0.4 Contact Map
3vaxA 2 0.8835 100 0.401 Contact Map
3a9zA 2 0.9519 100 0.402 Contact Map
3lvmA 2 0.9747 100 0.412 Contact Map
4ixoA 2 0.8987 100 0.422 Contact Map
1kmjA 2 0.9165 100 0.427 Contact Map
3caiA 2 0.8987 100 0.431 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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